FastQCFastQC Report
Wed 25 May 2016
SRR1032926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032926_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963452
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53180.27084950383304507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44800.22816957073562277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24580.1251876796580716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48200.038.6003271
GTATCAA74600.032.8126831
ATCAACG87500.027.6924863
TCAACGC87350.027.6861764
GTACATG68350.027.4269311
CAACGCA88450.027.3376755
AACGCAG90650.026.7254266
TATCAAC90900.026.7077642
TACGACC1606.8497684E-826.4658164
TACATGG67400.026.386612
ACACCGT4800.025.481056
ACATGGG69700.025.2465253
TACACCG3750.023.8351925
ACGCAGA103600.023.3835567
ACCGTTC2853.6379788E-1223.1071438
CGCAGAG105250.023.0169728
ACCGTCC2103.8242433E-822.399788
GCAGAGT114550.021.1591349
TATACTG11400.020.6329575
CATGGGG48900.020.590544
Produced by FastQC (version 0.11.5)