##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032926_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1963452 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.85839022293389 34.0 31.0 34.0 31.0 34.0 2 32.90515072433652 34.0 31.0 34.0 31.0 34.0 3 32.978496036572324 34.0 31.0 34.0 31.0 34.0 4 36.384096479058314 37.0 37.0 37.0 35.0 37.0 5 36.36725522192547 37.0 37.0 37.0 35.0 37.0 6 36.42583062891275 37.0 37.0 37.0 35.0 37.0 7 36.39227085765275 37.0 37.0 37.0 35.0 37.0 8 36.33457298676005 37.0 37.0 37.0 35.0 37.0 9 38.18002120754671 39.0 39.0 39.0 37.0 39.0 10-11 38.189062681440646 39.0 39.0 39.0 37.0 39.0 12-13 38.18233193375748 39.0 39.0 39.0 37.0 39.0 14-15 39.74199776719777 41.0 40.0 41.0 37.0 41.0 16-17 39.75250502686086 41.0 40.0 41.0 37.0 41.0 18-19 39.774482391217106 41.0 40.0 41.0 37.0 41.0 20-21 39.78049909037756 41.0 40.0 41.0 37.0 41.0 22-23 39.74147751001807 41.0 40.0 41.0 37.0 41.0 24-25 39.720057582258185 41.0 40.0 41.0 37.0 41.0 26-27 39.63857939995477 41.0 40.0 41.0 37.0 41.0 28-29 39.579986931180386 41.0 40.0 41.0 37.0 41.0 30-31 39.46704579485518 41.0 39.5 41.0 36.0 41.0 32-33 39.33830977278792 41.0 39.0 41.0 35.5 41.0 34-35 39.24037664276998 41.0 39.0 41.0 35.0 41.0 36-37 39.14726970661875 41.0 39.0 41.0 35.0 41.0 38-39 39.049445313661856 41.0 39.0 41.0 35.0 41.0 40-41 38.95193669109304 41.0 39.0 41.0 35.0 41.0 42-43 38.81252559268064 41.0 39.0 41.0 35.0 41.0 44-45 38.63066196678095 41.0 39.0 41.0 35.0 41.0 46-47 38.39585128640782 41.0 38.0 41.0 35.0 41.0 48-49 38.183973939775456 41.0 38.0 41.0 34.0 41.0 50-51 38.0181947406914 40.0 38.0 41.0 34.0 41.0 52-53 37.87233657863803 40.0 37.5 41.0 34.0 41.0 54-55 37.71535438605069 40.0 37.0 41.0 34.0 41.0 56-57 37.496207954154215 40.0 37.0 41.0 33.0 41.0 58-59 37.31820996897301 40.0 36.0 41.0 33.0 41.0 60-61 37.11707594583417 39.5 36.0 41.0 33.0 41.0 62-63 36.86855522824088 39.0 35.0 41.0 33.0 41.0 64-65 36.574029056987385 39.0 35.0 41.0 32.0 41.0 66-67 36.29017999930734 38.0 35.0 41.0 32.0 41.0 68-69 35.991181347952484 37.0 35.0 40.0 32.0 41.0 70-71 35.66352169546289 37.0 35.0 40.0 31.0 41.0 72-73 35.324585474969595 36.5 35.0 39.0 31.0 41.0 74-75 34.980206035085146 36.0 35.0 39.0 31.0 41.0 76-77 34.645642979813104 35.5 35.0 38.5 31.0 40.0 78-79 34.310254847075456 35.0 35.0 37.0 31.0 39.0 80-81 33.98986784499952 35.0 34.0 37.0 30.0 39.0 82-83 33.69597066798679 35.0 34.0 36.0 30.0 39.0 84-85 33.432291953151896 35.0 34.0 36.0 30.0 37.0 86-87 33.29924464667331 35.0 34.0 36.0 30.0 37.0 88-89 33.12309086241986 35.0 34.0 35.0 30.0 37.0 90-91 32.95895494262146 35.0 34.0 35.0 30.0 36.0 92-93 32.803981711801455 35.0 34.0 35.0 30.0 36.0 94-95 32.708532217747106 35.0 34.0 35.0 29.5 36.0 96-97 32.65328793370044 35.0 34.0 35.0 30.0 36.0 98-99 32.54865053996736 35.0 34.0 35.0 30.0 35.5 100 32.47042810315709 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12055981364346735 1101 2 0.15476812658727113 1101 3 0.18530715383305818 1101 4 0.10240892706895721 1101 5 0.031779218907985296 1101 6 0.0934767700879604 1101 7 0.06908809808690819 1101 8 -0.018935642579862133 1101 9 0.01513380866992975 1101 10-11 0.10033956130002508 1101 12-13 0.16253775583355434 1101 14-15 0.2023239768924796 1101 16-17 0.1604219330455905 1101 18-19 0.167845444539509 1101 20-21 0.17461157681709238 1101 22-23 0.18286588857053232 1101 24-25 0.17078265546307847 1101 26-27 0.14936212753094225 1101 28-29 0.12116885767364494 1101 30-31 0.16914043706013615 1101 32-33 0.2085358934903141 1101 34-35 0.22371147705775485 1101 36-37 0.20985055433730793 1101 38-39 0.19749009196098655 1101 40-41 0.19403827392299888 1101 42-43 0.20885909479067521 1101 44-45 0.19140627173045743 1101 46-47 0.20320168349702783 1101 48-49 0.10046628352473164 1101 50-51 0.12571725568489 1101 52-53 0.20445819037892932 1101 54-55 0.15623561177662992 1101 56-57 0.21007148949296095 1101 58-59 0.18328588122731304 1101 60-61 0.2188607764590813 1101 62-63 0.15903477112316722 1101 64-65 0.10759187459099451 1101 66-67 0.15725881647954054 1101 68-69 0.16276861435398615 1101 70-71 0.11953812145345921 1101 72-73 0.17762538952241158 1101 74-75 0.13886661581937432 1101 76-77 0.20838540369294378 1101 78-79 0.2511914753881257 1101 80-81 0.23230645968305197 1101 82-83 0.12593108458629132 1101 84-85 0.18478654779983827 1101 86-87 0.1738193677991191 1101 88-89 0.16464673211123682 1101 90-91 0.21167164733372346 1101 92-93 0.13068916401475406 1101 94-95 0.1560831158597793 1101 96-97 0.14110391646386233 1101 98-99 0.18717250992278878 1101 100 0.219809861358236 1102 1 0.22279114802665845 1102 2 0.25680560627569804 1102 3 0.21944591257253165 1102 4 0.17480084332818535 1102 5 0.14417037757415585 1102 6 0.1628151963777782 1102 7 0.201130719381176 1102 8 0.10050781618897275 1102 9 0.1661828243838528 1102 10-11 0.22318759314567416 1102 12-13 0.19967722370241603 1102 14-15 0.29103241441806915 1102 16-17 0.2859638766840149 1102 18-19 0.3135698622651759 1102 20-21 0.3187670527387283 1102 22-23 0.3205511855275134 1102 24-25 0.27882378162516375 1102 26-27 0.30123878581023433 1102 28-29 0.2653009373390134 1102 30-31 0.26711142458826487 1102 32-33 0.2866564109486589 1102 34-35 0.32583864239519045 1102 36-37 0.34743962026406905 1102 38-39 0.3374572202209336 1102 40-41 0.243776486527004 1102 42-43 0.22530694147166486 1102 44-45 0.2956141719777321 1102 46-47 0.32228612114294464 1102 48-49 0.2553029860118059 1102 50-51 0.3120248473801155 1102 52-53 0.3770508409463673 1102 54-55 0.4158546761603432 1102 56-57 0.40162791987043533 1102 58-59 0.36225108709487586 1102 60-61 0.3867115072300109 1102 62-63 0.36885977826737815 1102 64-65 0.38958611576963165 1102 66-67 0.37449890496195337 1102 68-69 0.3685670979423463 1102 70-71 0.4443334459901678 1102 72-73 0.4318549591594234 1102 74-75 0.39275039435933223 1102 76-77 0.4533772122179869 1102 78-79 0.4546004770420211 1102 80-81 0.39855496469012763 1102 82-83 0.4346977453667549 1102 84-85 0.3990626926984291 1102 86-87 0.4018387243017969 1102 88-89 0.3520712165162365 1102 90-91 0.2746565974133546 1102 92-93 0.3062705470565916 1102 94-95 0.28849269629248653 1102 96-97 0.28703124100725574 1102 98-99 0.4077379305902582 1102 100 0.4213112325459676 1103 1 0.15528803330921903 1103 2 0.17301784365444206 1103 3 0.12608931203371299 1103 4 0.07759956336567342 1103 5 0.09609644429832542 1103 6 0.07779432510878337 1103 7 0.06311519371271146 1103 8 0.01655979203793123 1103 9 0.0736610746048143 1103 10-11 0.13622148766057052 1103 12-13 0.14470831763377845 1103 14-15 0.17614925625431255 1103 16-17 0.16913255593733112 1103 18-19 0.20310574274520832 1103 20-21 0.223859881967023 1103 22-23 0.16000134472417926 1103 24-25 0.18083583584786567 1103 26-27 0.24412924359383226 1103 28-29 0.2348764040141944 1103 30-31 0.20540940454758072 1103 32-33 0.2371399907972318 1103 34-35 0.1760268565173746 1103 36-37 0.17706124864899664 1103 38-39 0.21533793452946526 1103 40-41 0.1881431074046631 1103 42-43 0.19446152196980648 1103 44-45 0.16552107653426873 1103 46-47 0.18640577421133742 1103 48-49 0.033751271053709786 1103 50-51 0.0356929729058848 1103 52-53 0.08498897786616055 1103 54-55 0.08866891654198383 1103 56-57 0.14709627221153454 1103 58-59 0.16035403782377244 1103 60-61 0.11050821468083427 1103 62-63 0.10978756474151652 1103 64-65 0.12158331518608634 1103 66-67 0.13745187006647086 1103 68-69 0.24424887498182102 1103 70-71 0.19872346718089773 1103 72-73 0.18616391783217523 1103 74-75 0.2040311289002119 1103 76-77 0.2156354888474823 1103 78-79 0.17739435196348552 1103 80-81 0.183110758064565 1103 82-83 0.21914825064314414 1103 84-85 0.22343682084376582 1103 86-87 0.2636640019642158 1103 88-89 0.16544759684187227 1103 90-91 0.22794275741932069 1103 92-93 0.23203391904436899 1103 94-95 0.15967224244887035 1103 96-97 0.10680064543069534 1103 98-99 0.08014286515457059 1103 100 0.09465191341787715 1104 1 0.15364900220965438 1104 2 0.1748217966867074 1104 3 0.16397741982515868 1104 4 0.13196585561497898 1104 5 0.08135344233708963 1104 6 0.09837263437243138 1104 7 0.0812018445876248 1104 8 0.029490563032048556 1104 9 0.10493702274673922 1104 10-11 0.06628949407915741 1104 12-13 0.14286615874836883 1104 14-15 0.15725168614657292 1104 16-17 0.17126221386759255 1104 18-19 0.20230023600824865 1104 20-21 0.20127481026737115 1104 22-23 0.2087857618302138 1104 24-25 0.1763823069005639 1104 26-27 0.2545174150617058 1104 28-29 0.2287083874073872 1104 30-31 0.27287775736590447 1104 32-33 0.2918517276353185 1104 34-35 0.2816743183752024 1104 36-37 0.2896924220780903 1104 38-39 0.22226847628846258 1104 40-41 0.2583457257791295 1104 42-43 0.28425705697910786 1104 44-45 0.3307311450500947 1104 46-47 0.28358331681086213 1104 48-49 0.3532044179424716 1104 50-51 0.380907053036303 1104 52-53 0.35014619495181165 1104 54-55 0.3788458733184257 1104 56-57 0.3778752258805653 1104 58-59 0.3630233534295897 1104 60-61 0.369650238572234 1104 62-63 0.3708777687607707 1104 64-65 0.39617578608596205 1104 66-67 0.4140953585155671 1104 68-69 0.4441100603820871 1104 70-71 0.452527178242768 1104 72-73 0.4616561365734242 1104 74-75 0.4013627684407268 1104 76-77 0.46287161375515495 1104 78-79 0.3330628270405356 1104 80-81 0.41596779176212095 1104 82-83 0.44523106819745806 1104 84-85 0.4200415940283406 1104 86-87 0.41569910040797 1104 88-89 0.3847093960828474 1104 90-91 0.4242087720394281 1104 92-93 0.46924482808896784 1104 94-95 0.3829523422934997 1104 96-97 0.4675447680969427 1104 98-99 0.49181921063736667 1104 100 0.5025312338631025 1105 1 0.051464840883241436 1105 2 0.055812975214870164 1105 3 0.03578649187652161 1105 4 0.04522353570682469 1105 5 0.040870711117754865 1105 6 0.03500904299256291 1105 7 0.07986728923221875 1105 8 0.0037047597759780615 1105 9 0.012938748527908217 1105 10-11 0.05143887896492316 1105 12-13 0.05535375774312712 1105 14-15 0.056904397061167344 1105 16-17 0.11750614133602966 1105 18-19 0.14295780007299896 1105 20-21 0.08772925622579919 1105 22-23 0.14227134010097586 1105 24-25 0.12161002226368112 1105 26-27 0.10394501878528928 1105 28-29 0.084325890436034 1105 30-31 0.11345780753055834 1105 32-33 0.2225022593855499 1105 34-35 0.2664744032017339 1105 36-37 0.22363881804736963 1105 38-39 0.18778411435217635 1105 40-41 0.1706799423159211 1105 42-43 0.09997293928473283 1105 44-45 0.17439889317380164 1105 46-47 0.22628622406962506 1105 48-49 0.28602418106195415 1105 50-51 0.2846185794507434 1105 52-53 0.242034596873566 1105 54-55 0.2643445582634243 1105 56-57 0.4082233802941104 1105 58-59 0.30028298111911766 1105 60-61 0.3189691887707724 1105 62-63 0.35047004077119226 1105 64-65 0.3809503376388008 1105 66-67 0.3780441432779895 1105 68-69 0.40287467650546915 1105 70-71 0.40283284626389815 1105 72-73 0.3541542291952169 1105 74-75 0.28170982595944594 1105 76-77 0.2939579174390161 1105 78-79 0.31498807244027915 1105 80-81 0.3297322981960207 1105 82-83 0.31694745263715873 1105 84-85 0.29282464773475425 1105 86-87 0.23829866798765664 1105 88-89 0.257683370425255 1105 90-91 0.26019667207086883 1105 92-93 0.2931429363110425 1105 94-95 0.17326347041325363 1105 96-97 0.1473522143064443 1105 98-99 0.17978054631399232 1105 100 0.15798986995971376 1106 1 0.11241119438894742 1106 2 0.15667051937348475 1106 3 0.09866101812444583 1106 4 0.028699686084564746 1106 5 0.06189053987994697 1106 6 0.07461866319685129 1106 7 0.05453133000520438 1106 8 0.05433721374873812 1106 9 0.05080468055295739 1106 10-11 0.09574810076814799 1106 12-13 0.03443657229286856 1106 14-15 0.09285101207849067 1106 16-17 0.09911801101575435 1106 18-19 0.12264922349027074 1106 20-21 0.11008077349246292 1106 22-23 0.16904888214847347 1106 24-25 0.16216542302548476 1106 26-27 0.16158687576506026 1106 28-29 0.18503657204468027 1106 30-31 0.15699444079189107 1106 32-33 0.18792269637621928 1106 34-35 0.2086652382178542 1106 36-37 0.15314627992491836 1106 38-39 0.1904821416511382 1106 40-41 0.21162030007332788 1106 42-43 0.26031492754334096 1106 44-45 0.2490035232608534 1106 46-47 0.2548294450464752 1106 48-49 0.33975679588813534 1106 50-51 0.38314320219645737 1106 52-53 0.2902439673580375 1106 54-55 0.2914715154996159 1106 56-57 0.30683484867235933 1106 58-59 0.2736101625896339 1106 60-61 0.3208398289559824 1106 62-63 0.3635852052596178 1106 64-65 0.35281341062859894 1106 66-67 0.29526091549233513 1106 68-69 0.2955868587153532 1106 70-71 0.29640938382148363 1106 72-73 0.3972761882310891 1106 74-75 0.3722786707960424 1106 76-77 0.365998366630393 1106 78-79 0.24193753460596668 1106 80-81 0.28504097225125946 1106 82-83 0.26592992279986305 1106 84-85 0.2763758638351206 1106 86-87 0.2594282127624865 1106 88-89 0.28115903106305495 1106 90-91 0.2549816548372519 1106 92-93 0.239212166996154 1106 94-95 0.2556528260343498 1106 96-97 0.25515723076503605 1106 98-99 0.23711685073634214 1106 100 0.29602889890524864 1107 1 0.05892871799705546 1107 2 0.0596922378456739 1107 3 0.07241727984055046 1107 4 0.056903648747528734 1107 5 0.05977051772438813 1107 6 0.04476498967589748 1107 7 0.05966842615081447 1107 8 0.1027749088256158 1107 9 0.10769498823150059 1107 10-11 0.08329669251288152 1107 12-13 0.05374296027220993 1107 14-15 0.0417723425984633 1107 16-17 0.0858054192038864 1107 18-19 0.06925262403294141 1107 20-21 0.07111989129837326 1107 22-23 0.0595150938858211 1107 24-25 0.06804022158256373 1107 26-27 0.06359531538691243 1107 28-29 0.03133887448921513 1107 30-31 0.04691860467588782 1107 32-33 -0.0107418655582876 1107 34-35 0.03211191760856735 1107 36-37 0.03249275070480451 1107 38-39 0.06567036388822345 1107 40-41 0.05663157443875377 1107 42-43 0.1159254504683318 1107 44-45 0.09816804530340306 1107 46-47 0.10637012553668512 1107 48-49 0.04547815409106448 1107 50-51 0.12113606624855322 1107 52-53 0.08676886005785889 1107 54-55 0.11993440804170774 1107 56-57 0.1599505719228418 1107 58-59 0.050853556676010214 1107 60-61 0.04777894048494602 1107 62-63 0.07665726118102612 1107 64-65 0.10101424861274211 1107 66-67 0.080674976822948 1107 68-69 0.06535522321615872 1107 70-71 0.06153912926937721 1107 72-73 0.01597466638143885 1107 74-75 0.09117828721240073 1107 76-77 0.1381484652566627 1107 78-79 0.12866137438788883 1107 80-81 0.09406343062163813 1107 82-83 0.10603879681874417 1107 84-85 0.11334729820065803 1107 86-87 0.0743017414445859 1107 88-89 0.1303628127751537 1107 90-91 0.11066517890475325 1107 92-93 0.10406428967394277 1107 94-95 0.22755198467031335 1107 96-97 0.12579650464394376 1107 98-99 0.1517792049323674 1107 100 0.0975023493669056 1108 1 0.05494470843357391 1108 2 0.04606182364268818 1108 3 0.006216249059761481 1108 4 0.011885103582706336 1108 5 0.02313560770376455 1108 6 0.05452815156845503 1108 7 0.06509199957288558 1108 8 0.05149757784334241 1108 9 0.010778507480857513 1108 10-11 0.036849363633571386 1108 12-13 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-0.20592024169215506 2214 40-41 -0.270966165392565 2214 42-43 -0.1288855987176376 2214 44-45 -0.12416566195489054 2214 46-47 -0.13920183127795127 2214 48-49 -0.14205352566298046 2214 50-51 -0.15090443635408946 2214 52-53 -0.16914241882487602 2214 54-55 -0.03325137331308525 2214 56-57 -0.054511718041126755 2214 58-59 -0.048489106975509344 2214 60-61 -0.07011574325169789 2214 62-63 -0.04320557364503941 2214 64-65 -0.14284210484726145 2214 66-67 -0.13026648314630052 2214 68-69 -0.14585629367279296 2214 70-71 -0.09196686243305408 2214 72-73 -0.14164806113409156 2214 74-75 -0.14427751545017742 2214 76-77 -0.1278187243114246 2214 78-79 -0.10237350746962903 2214 80-81 -0.14050088744011902 2214 82-83 -0.11816898675585463 2214 84-85 -0.10684405641672612 2214 86-87 -0.10273333034307797 2214 88-89 -0.13248269686083347 2214 90-91 -0.08191359758901484 2214 92-93 -0.0493387242799912 2214 94-95 -0.16949282103708185 2214 96-97 -0.03730926863882189 2214 98-99 0.0144413132756398 2214 100 -0.02466962050542776 2215 1 -0.08630914755240582 2215 2 -0.14465140391355646 2215 3 -0.11580566513524104 2215 4 -0.066588445369689 2215 5 -0.12383109193356034 2215 6 -0.08526404296745227 2215 7 -0.10929397841355382 2215 8 0.007015420388135851 2215 9 -0.0880795448115066 2215 10-11 -0.09353828998484914 2215 12-13 -0.15728691762530644 2215 14-15 -0.15045263971551037 2215 16-17 -0.14888784310972625 2215 18-19 -0.11090681433240945 2215 20-21 -0.09052492049476513 2215 22-23 -0.12789819865481888 2215 24-25 -0.19982725726310946 2215 26-27 -0.16461728211591264 2215 28-29 -0.20023378061952002 2215 30-31 -0.1592111533241649 2215 32-33 -0.1417958837312554 2215 34-35 -0.1365860579158138 2215 36-37 -0.13886961297499312 2215 38-39 -0.1675933737889821 2215 40-41 -0.22101288822765497 2215 42-43 -0.22435424138139126 2215 44-45 -0.27351381526641205 2215 46-47 -0.13049168459350824 2215 48-49 -0.14409392449919523 2215 50-51 -0.25985249066369676 2215 52-53 -0.21266587621364863 2215 54-55 -0.25297169294778854 2215 56-57 -0.27569383102451894 2215 58-59 -0.2582160435738885 2215 60-61 -0.19879295665130314 2215 62-63 -0.21747568719415966 2215 64-65 -0.27465328398007927 2215 66-67 -0.24647829669946475 2215 68-69 -0.2241127490007102 2215 70-71 -0.2319532749274913 2215 72-73 -0.25336109705764187 2215 74-75 -0.2813706479510856 2215 76-77 -0.31353365945507505 2215 78-79 -0.26811885033947647 2215 80-81 -0.27446939877483345 2215 82-83 -0.21740500526315287 2215 84-85 -0.22885000663291066 2215 86-87 -0.31736389162323064 2215 88-89 -0.3403892107817157 2215 90-91 -0.2623193033311324 2215 92-93 -0.20320282901995768 2215 94-95 -0.27534984411059327 2215 96-97 -0.268569118317572 2215 98-99 -0.2972457093937706 2215 100 -0.3188754838423975 2216 1 -0.19806169951251462 2216 2 -0.20799582520144355 2216 3 -0.24077136293470858 2216 4 -0.19775078934102908 2216 5 -0.25498972755890037 2216 6 -0.2323005800645106 2216 7 -0.16414875958405872 2216 8 -0.13827943020260136 2216 9 -0.21761129814221647 2216 10-11 -0.2742243587178095 2216 12-13 -0.312335027234127 2216 14-15 -0.39679953261600787 2216 16-17 -0.41205795668400214 2216 18-19 -0.3682848004780297 2216 20-21 -0.2663379908074788 2216 22-23 -0.31167795504120477 2216 24-25 -0.3440584306085128 2216 26-27 -0.3770155034695577 2216 28-29 -0.3703493635838626 2216 30-31 -0.4690342683100823 2216 32-33 -0.46094062171188455 2216 34-35 -0.4542747292896152 2216 36-37 -0.5014366876833307 2216 38-39 -0.4284993103564858 2216 40-41 -0.45894755273359067 2216 42-43 -0.45533181701311065 2216 44-45 -0.5106958400480437 2216 46-47 -0.5723219511246924 2216 48-49 -0.6047133758864973 2216 50-51 -0.5597234093198935 2216 52-53 -0.4924188632863604 2216 54-55 -0.43355817713911193 2216 56-57 -0.5168539839814272 2216 58-59 -0.5470061153427466 2216 60-61 -0.6229563537929081 2216 62-63 -0.6726172162193862 2216 64-65 -0.6439638313813987 2216 66-67 -0.6938966543719118 2216 68-69 -0.7181523553721405 2216 70-71 -0.6933564234368674 2216 72-73 -0.623514134316629 2216 74-75 -0.680597195134844 2216 76-77 -0.6552019639745623 2216 78-79 -0.6490413879359238 2216 80-81 -0.5677166706682826 2216 82-83 -0.5540816442902781 2216 84-85 -0.5945331735851198 2216 86-87 -0.5224701913614211 2216 88-89 -0.5513633500127995 2216 90-91 -0.6171177794481437 2216 92-93 -0.6707625272100373 2216 94-95 -0.6562627245559867 2216 96-97 -0.6394727866134389 2216 98-99 -0.6383028321375512 2216 100 -0.5360555401103682 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 5.0 4 4.0 5 20.0 6 40.0 7 120.0 8 233.0 9 460.0 10 731.0 11 1128.0 12 2008.0 13 3186.0 14 3566.0 15 4486.0 16 5932.0 17 8776.0 18 10981.0 19 6195.0 20 4259.0 21 4053.0 22 4159.0 23 4268.0 24 4678.0 25 5546.0 26 6928.0 27 8951.0 28 11417.0 29 14751.0 30 18114.0 31 23478.0 32 30051.0 33 39565.0 34 55635.0 35 89002.0 36 171740.0 37 394161.0 38 686033.0 39 337508.0 40 1278.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.387390530419744 19.014843807695147 13.055929756117438 23.54183590576767 2 15.977052531754202 19.749001879862956 39.90815104706482 24.365794541318024 3 18.911119622215615 24.98579030358838 29.64697817309142 26.456111901104588 4 12.505462980602216 16.287271790034097 37.85593419769827 33.351331031665424 5 12.821616324801038 37.70663966646328 35.13172276033619 14.340021248399495 6 31.39442020039035 37.32346661451139 17.30353148263596 13.9785817024623 7 28.11340521748342 32.37827340525823 21.68844735023329 17.819874027025058 8 26.055002636805312 35.651545922744155 19.566778497133175 18.726672943317357 9 25.745355312601543 16.19480438784044 20.61569929297055 37.444141006587465 10-11 24.631210745157 26.329062284181127 28.045325274058136 20.994401696603738 12-13 25.50829495915738 23.660019104113346 28.600273548901008 22.23141238782827 14-15 22.389804270494352 25.376435706647914 26.17803513663071 26.055724886227022 16-17 21.64804639991199 29.023245793632846 26.700168886226912 22.62853892022825 18-19 21.60713112487261 27.42116041720462 29.418207450257178 21.55350100766559 20-21 22.50773128143698 26.6357924716265 29.358140662465903 21.498335584470617 22-23 22.203077900948255 26.39004791824181 29.458806788158693 21.948067392651247 24-25 21.969534145104213 26.53836829570897 29.323856448159457 22.16824111102736 26-27 21.770928462321663 26.58205927339237 29.463445765716944 22.18356649856902 28-29 21.85303144982117 26.723077973104715 29.47764957077763 21.946241006296482 30-31 21.938166469326333 26.909742075463384 29.198287175900656 21.953804279309626 32-33 21.79082018853527 26.69822844492531 29.5429145437856 21.968036822753824 34-35 21.923686288494356 26.512086400570574 29.472222929774066 22.092004381161008 36-37 22.144201304158535 26.76648215246025 29.071368505414547 22.01794803796667 38-39 22.010191535858645 26.990500909758385 29.100778606436634 21.898528947946332 40-41 21.873964119982585 26.791637694936536 29.189082866069967 22.145315319010916 42-43 21.906279003037085 26.840993660296043 28.96815061982723 22.284576716839638 44-45 21.951807099913808 26.979988584134396 28.875919636181102 22.192284679770694 46-47 22.220046630743912 27.148118262093334 28.484169385093715 22.14766572206904 48-49 22.146546710996116 27.14355240820212 28.35386769847103 22.356033182330734 50-51 22.10541585900905 27.24758697016152 28.232582958536856 22.414414212292577 52-53 22.176742032745224 27.268534770548108 28.155092544792502 22.39963065191416 54-55 22.17355189852505 27.46382996949755 28.063961320732588 22.298656811244808 56-57 22.037976425964068 27.2195230132349 28.223688235414073 22.518812325386953 58-59 22.106232792111634 26.861434092226382 28.455914925678233 22.576418189983755 60-61 22.247445100307246 27.209407720035912 28.186226405379628 22.35692077427722 62-63 21.94945022061779 27.153741199056608 28.311031960076477 22.58577662024912 64-65 22.031922365264016 27.124180842811967 28.33982105996024 22.50407573196377 66-67 21.939089929369292 27.07522261812359 28.229159663694354 22.756527788812765 68-69 22.166297461383692 27.188906881580728 28.214804389107652 22.42999126792793 70-71 22.255191367041313 26.996203625044057 28.101807428956754 22.646797578957877 72-73 22.100667599717234 27.104176725481448 28.170385626946825 22.624770047854494 74-75 22.090354131397152 27.290914165459608 27.979904779948782 22.638826923194454 76-77 22.139456971562577 27.168127147525674 27.849147279675613 22.84326860123614 78-79 22.159147338028806 27.133704890012535 28.053284752204156 22.653863019754503 80-81 22.143958521936543 27.159447858931802 27.984985592943023 22.711608026188628 82-83 21.993687648081035 27.149530520735926 28.12531195058499 22.73146988059805 84-85 22.00965442496175 27.111154232443674 27.97091551003029 22.90827583256428 86-87 22.108045875734586 27.137294368617848 27.899886652699646 22.85477310294792 88-89 21.76820873986918 27.150830761739986 27.956279772378974 23.12468072601186 90-91 21.826144619057903 27.08979305792667 27.955464061182166 23.128598261833265 92-93 22.007465483978088 27.16021810744022 27.872133341753948 22.960183066827746 94-95 21.718306222237725 26.953242877846385 28.04996960681378 23.27848129310211 96-97 21.72318464577075 26.930585307668252 28.05210838495176 23.294121661609232 98-99 21.673981168870522 27.076968052678673 27.94388553623187 23.30516524221893 100 21.805065771915995 26.73373222263646 27.81784326787719 23.64335873757036 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 100.0 1 105.0 2 353.0 3 619.0 4 402.0 5 219.5 6 558.0 7 1229.5 8 2058.5 9 2349.0 10 1714.0 11 1085.5 12 1006.0 13 1140.0 14 1313.5 15 1453.5 16 1570.5 17 1766.0 18 1978.5 19 2215.0 20 2468.0 21 2897.0 22 3731.0 23 5056.5 24 6748.0 25 8743.0 26 11529.0 27 15267.0 28 19689.0 29 24441.0 30 28778.0 31 33232.5 32 37611.5 33 41574.5 34 45704.5 35 49246.5 36 52827.5 37 56359.5 38 59198.5 39 61062.0 40 62987.5 41 65398.0 42 66550.5 43 67179.5 44 66978.5 45 66603.0 46 67439.0 47 67602.0 48 67873.5 49 66454.5 50 63736.5 51 62117.5 52 58361.5 53 55325.0 54 54068.0 55 51811.0 56 48008.5 57 43733.0 58 40340.5 59 37458.0 60 33800.0 61 29394.5 62 25943.5 63 22446.0 64 18637.0 65 15181.5 66 12117.5 67 9377.0 68 7167.0 69 5531.0 70 4038.0 71 2898.5 72 2199.5 73 1647.0 74 1223.5 75 896.5 76 564.5 77 391.0 78 280.0 79 156.0 80 86.0 81 44.5 82 29.0 83 14.0 84 3.5 85 4.0 86 3.0 87 0.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.5651495427441057E-4 2 5.602377852883595E-4 3 2.037228310139489E-4 4 0.01212150844532996 5 5.093070775348723E-4 6 0.0042781794512929275 7 7.639606163023084E-4 8 0.13985572349107592 9 0.0013241984015906678 10-11 0.0 12-13 2.5465353876743614E-5 14-15 1.2732676938371807E-4 16-17 0.0 18-19 1.5279212326046166E-4 20-21 0.0 22-23 1.7825747713720529E-4 24-25 4.074456620278978E-4 26-27 0.005551447145130108 28-29 0.006824714838967288 30-31 0.01331838007753691 32-33 0.016195965065608938 34-35 0.025567215292250586 36-37 0.03720488201392242 38-39 0.04293458663618973 40-41 0.05350270849503833 42-43 0.06992786174553796 44-45 0.10932276419286034 46-47 0.11711516247914387 48-49 0.1134481515208928 50-51 0.11594375620081368 52-53 0.10993393268590218 54-55 0.11703876641751365 56-57 0.10043535568987681 58-59 0.09060572909345378 60-61 0.09315226448112814 62-63 0.09175167001790724 64-65 5.093070775348723E-5 66-67 0.0 68-69 2.5465353876743614E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 2.5465353876743614E-5 78-79 5.093070775348723E-5 80-81 2.291881848906925E-4 82-83 0.0 84-85 0.0 86-87 8.912873856860265E-4 88-89 6.620992007953339E-4 90-91 2.037228310139489E-4 92-93 8.148913240557956E-4 94-95 9.422180934395137E-4 96-97 3.055842465209233E-4 98-99 5.093070775348723E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1963452.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.449014496249156 #Duplication Level Percentage of deduplicated Percentage of total 1 78.192210606475 48.83026493657525 2 13.085121551108184 16.343058908606743 3 3.8358566952370934 7.186364110991869 4 1.6154693628914556 4.035378786458196 5 0.8708251024093571 2.7191084722029797 6 0.5594416115162396 2.0961946384429586 7 0.36444390852892034 1.5931414048754575 8 0.24895073311352536 1.243738235284672 9 0.19137552037933214 1.0756091381756525 >10 0.9768677146757233 10.69754526409607 >50 0.04646248855563282 1.976602798669385 >100 0.012159256076230088 1.2633947937752135 >500 4.0772451665191584E-4 0.18806228953693818 >1k 3.261796133215326E-4 0.48072245145645714 >5k 8.154490333038316E-5 0.2708137708522711 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5318 0.27084950383304507 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4480 0.22816957073562277 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2458 0.1251876796580716 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.093070775348723E-5 0.0 10-11 0.0 0.0 0.0 5.093070775348723E-5 0.0 12-13 0.0 0.0 0.0 5.093070775348723E-5 0.0 14-15 0.0 0.0 0.0 5.093070775348723E-5 0.0 16-17 0.0 0.0 0.0 5.093070775348723E-5 0.0 18-19 0.0 5.093070775348723E-5 0.0 5.093070775348723E-5 0.0 20-21 0.0 5.093070775348723E-5 0.0 5.093070775348723E-5 0.0 22-23 0.0 5.093070775348723E-5 0.0 5.093070775348723E-5 0.0 24-25 0.0 5.093070775348723E-5 0.0 5.093070775348723E-5 0.0 26-27 0.0 5.093070775348723E-5 0.0 1.2732676938371807E-4 0.0 28-29 0.0 5.093070775348723E-5 0.0 7.384952624255647E-4 0.0 30-31 0.0 5.093070775348723E-5 0.0 0.004278179451292927 0.0 32-33 2.5465353876743614E-5 5.093070775348723E-5 0.0 0.011535805306164857 0.0 34-35 5.093070775348723E-5 7.639606163023084E-5 0.0 0.02149275867197161 0.0 36-37 5.093070775348723E-5 1.0186141550697446E-4 0.0 0.040693635495036296 0.0 38-39 5.093070775348723E-5 1.0186141550697446E-4 0.0 0.07682897264613547 0.0 40-41 5.093070775348723E-5 1.0186141550697446E-4 0.0 0.12289579780916468 0.0 42-43 5.093070775348723E-5 1.0186141550697446E-4 0.0 0.16830052377139854 0.0 44-45 1.527921232604617E-4 1.0186141550697446E-4 0.0 0.21918030081713227 0.0 46-47 1.527921232604617E-4 1.0186141550697446E-4 0.0 0.2819014674155518 0.0 48-49 1.527921232604617E-4 1.0186141550697446E-4 0.0 0.35511435981118966 0.0 50-51 2.037228310139489E-4 1.0186141550697446E-4 0.0 0.4407798102525552 0.0 52-53 2.037228310139489E-4 1.0186141550697446E-4 0.0 0.5268527063559486 0.0 54-55 2.037228310139489E-4 1.0186141550697446E-4 0.0 0.6178149504036767 0.0 56-57 2.037228310139489E-4 1.0186141550697446E-4 0.0 0.7238017532386837 0.0 58-59 2.037228310139489E-4 1.0186141550697446E-4 0.0 0.8460609172009298 0.0 60-61 2.037228310139489E-4 1.0186141550697446E-4 0.0 0.9818421840717269 0.0 62-63 2.037228310139489E-4 1.0186141550697446E-4 0.0 1.120271847745705 0.0 64-65 2.037228310139489E-4 1.0186141550697446E-4 0.0 1.2639219089644156 0.0 66-67 2.037228310139489E-4 1.0186141550697446E-4 0.0 1.4271293619604655 0.0 68-69 2.037228310139489E-4 1.0186141550697446E-4 0.0 1.6204368632388264 0.0 70-71 2.037228310139489E-4 1.0186141550697446E-4 0.0 1.8318502311235518 0.0 72-73 2.037228310139489E-4 1.0186141550697446E-4 0.0 2.050317502032135 0.0 74-75 2.037228310139489E-4 1.0186141550697446E-4 0.0 2.2641500785351516 0.0 76-77 2.291881848906925E-4 1.0186141550697446E-4 0.0 2.4964450365988062 0.0 78-79 2.5465353876743613E-4 1.0186141550697446E-4 0.0 2.7608263405471587 0.0 80-81 2.5465353876743613E-4 1.0186141550697446E-4 0.0 3.0501891566485964 0.0 82-83 2.5465353876743613E-4 1.0186141550697446E-4 0.0 3.3628273061933776 0.0 84-85 2.5465353876743613E-4 1.0186141550697446E-4 0.0 3.665432106310722 0.0 86-87 2.5465353876743613E-4 1.0186141550697446E-4 0.0 3.977637344839599 0.0 88 2.5465353876743613E-4 1.0186141550697446E-4 0.0 4.226688505754152 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4820 0.0 38.600327 1 GTATCAA 7460 0.0 32.812683 1 ATCAACG 8750 0.0 27.692486 3 TCAACGC 8735 0.0 27.686176 4 GTACATG 6835 0.0 27.426931 1 CAACGCA 8845 0.0 27.337675 5 AACGCAG 9065 0.0 26.725426 6 TATCAAC 9090 0.0 26.707764 2 TACGACC 160 6.8497684E-8 26.465816 4 TACATGG 6740 0.0 26.38661 2 ACACCGT 480 0.0 25.48105 6 ACATGGG 6970 0.0 25.246525 3 TACACCG 375 0.0 23.835192 5 ACGCAGA 10360 0.0 23.383556 7 ACCGTTC 285 3.6379788E-12 23.107143 8 CGCAGAG 10525 0.0 23.016972 8 ACCGTCC 210 3.8242433E-8 22.39978 8 GCAGAGT 11455 0.0 21.159134 9 TATACTG 1140 0.0 20.632957 5 CATGGGG 4890 0.0 20.59054 4 >>END_MODULE