FastQCFastQC Report
Wed 25 May 2016
SRR1032925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032925_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCC160.7850834151128557No Hit
GTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTA160.7850834151128557No Hit
GTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGC160.7850834151128557No Hit
ATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAA130.6378802747791953No Hit
ACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGG130.6378802747791953No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG120.5888125613346418RNA PCR Primer, Index 33 (96% over 29bp)
CAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCA110.5397448478900884No Hit
CTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTG110.5397448478900884No Hit
GTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTT100.49067713444553485No Hit
GTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCG90.4416094210009814No Hit
CTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCA90.4416094210009814No Hit
TGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACT80.39254170755642787No Hit
TTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTAC80.39254170755642787No Hit
CCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCT80.39254170755642787No Hit
CTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCT80.39254170755642787No Hit
GGGGTAGGAAATCCTCCAGGATCTTAGGAAATTTCACTCCGCTTCTCCTG80.39254170755642787No Hit
CTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGG70.34347399411187435No Hit
CCATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGC70.34347399411187435No Hit
CGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGC70.34347399411187435No Hit
GGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTG60.2944062806673209No Hit
GTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTT60.2944062806673209No Hit
GATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGA60.2944062806673209No Hit
CCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTC60.2944062806673209No Hit
ATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAA50.24533856722276742No Hit
GGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGC50.24533856722276742No Hit
CTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAA50.24533856722276742No Hit
ATTTATTATAGGCCAGTGATGTCTCAAAGAGTAGAGGAGCGTCTACTGGT40.19627085377821393No Hit
GAGTAGAGGAGCGTCTACTGGTCTTTCAACTCCTTCAGTCTTCTGATGGC40.19627085377821393No Hit
ATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAG40.19627085377821393No Hit
CTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACCTGACCA40.19627085377821393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.19627085377821393No Hit
GTACATGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAA40.19627085377821393No Hit
ATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCT40.19627085377821393No Hit
GTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATC40.19627085377821393No Hit
GCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGT40.19627085377821393No Hit
GGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGT40.19627085377821393No Hit
GTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATG40.19627085377821393No Hit
GAGCACGCCATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGG30.14720314033366044No Hit
TTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAAC30.14720314033366044No Hit
CCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACC30.14720314033366044No Hit
GTATCAACGCAGAGTACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGA30.14720314033366044No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA30.14720314033366044RNA PCR Primer, Index 33 (96% over 29bp)
GGCTGCGCTATGACTCCCGGAGCGGCACCCACAACATGTACCGGGAATAC30.14720314033366044No Hit
TCTTGCAGGTGGCTCTTCCTCCACATCACGACTGGGGTCTCGAAGAGGAC30.14720314033366044No Hit
AGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTC30.14720314033366044No Hit
CCCCTGGATGTTCCAAGACACACCTGCCAGGGTTTGTGGAGCAGGCTGAG30.14720314033366044No Hit
CGCCATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCT30.14720314033366044No Hit
TTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGA30.14720314033366044No Hit
CATCACGACTGGGGTCTCGAAGAGGACGGGGGTAGGAAATCCTCCAGGAT30.14720314033366044No Hit
GAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTG30.14720314033366044No Hit
ACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCC30.14720314033366044No Hit
TTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTA30.14720314033366044No Hit
GTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTG30.14720314033366044No Hit
CCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGC30.14720314033366044No Hit
GTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCC30.14720314033366044No Hit
ACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTC30.14720314033366044No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)