FastQCFastQC Report
Wed 25 May 2016
SRR1032924_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032924_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091704
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21800.043.98091
GTATCAA36750.039.5176931
TCAACGC39550.034.9382934
ATCAACG39850.034.675273
CAACGCA41350.033.41745
AACGCAG42500.032.5131656
TATCAAC45850.030.7519282
GTACATG88100.026.7804741
TACATGG87550.025.714012
ACGCAGA54550.025.2449047
CGCAGAG55700.024.7236888
ACATGGG88600.024.295883
GCAGAGT63750.021.4537539
GAGTACA66350.020.5422271
CATGGGG58550.019.9079074
TACCGTC2151.1948741E-619.6745557
AGTACAT65350.019.4901052
CATGGGA26950.018.3118134
TATAACG1600.001279215117.62472
CAGAGTA61050.017.59101710-11
Produced by FastQC (version 0.11.5)