##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032924_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2091704
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60718103517515	33.0	31.0	34.0	30.0	34.0
2	31.63694671903864	34.0	31.0	34.0	30.0	34.0
3	31.786376083805358	34.0	31.0	34.0	30.0	34.0
4	35.04403730164498	37.0	35.0	37.0	33.0	37.0
5	35.14827097906778	37.0	35.0	37.0	33.0	37.0
6	35.20604110333011	37.0	37.0	37.0	33.0	37.0
7	35.12363412796457	37.0	36.0	37.0	33.0	37.0
8	35.12506071604778	37.0	37.0	37.0	33.0	37.0
9	36.72742940683768	39.0	38.0	39.0	33.0	39.0
10-11	36.72722765745058	39.0	38.0	39.0	33.5	39.0
12-13	36.668406476250944	39.0	38.0	39.0	33.0	39.0
14-15	38.14296143240152	41.0	39.0	41.0	33.5	41.0
16-17	38.175466031522625	41.0	39.0	41.0	34.0	41.0
18-19	38.203245296657656	41.0	39.0	41.0	33.5	41.0
20-21	38.26022993693181	41.0	39.0	41.0	34.0	41.0
22-23	38.26736670198078	41.0	39.0	41.0	34.0	41.0
24-25	38.317325969640066	41.0	39.0	41.0	34.0	41.0
26-27	38.30863114475088	41.0	39.0	41.0	33.5	41.0
28-29	38.29161176724814	41.0	39.0	41.0	33.0	41.0
30-31	38.248802889892644	41.0	39.0	41.0	33.0	41.0
32-33	38.18780071176418	41.0	39.0	41.0	33.0	41.0
34-35	38.109113670003026	41.0	39.0	41.0	33.0	41.0
36-37	38.01213125757755	41.0	38.0	41.0	33.0	41.0
38-39	37.910542313826426	41.0	38.0	41.0	33.0	41.0
40-41	37.81953445611808	40.5	38.0	41.0	32.5	41.0
42-43	37.696957839158884	40.0	38.0	41.0	32.0	41.0
44-45	37.568329457705296	40.0	38.0	41.0	32.0	41.0
46-47	37.442704847339776	40.0	38.0	41.0	31.5	41.0
48-49	37.301917957799	40.0	37.0	41.0	31.0	41.0
50-51	37.159815394530014	40.0	37.0	41.0	31.0	41.0
52-53	36.997775258832036	40.0	36.5	41.0	31.0	41.0
54-55	36.823273751926656	40.0	36.0	41.0	30.0	41.0
56-57	36.63337929267239	40.0	35.5	41.0	30.0	41.0
58-59	36.41800417267453	39.0	35.0	41.0	30.0	41.0
60-61	36.16625727158336	39.0	35.0	41.0	30.0	41.0
62-63	35.90360347353163	39.0	35.0	41.0	30.0	41.0
64-65	35.61694173745425	38.0	35.0	40.0	29.5	41.0
66-67	35.309763714177535	37.5	35.0	40.0	29.0	41.0
68-69	34.985441295709144	37.0	34.5	40.0	29.0	41.0
70-71	34.638075941911474	36.5	34.0	39.0	29.0	41.0
72-73	34.34352637849332	36.0	34.0	39.0	28.5	41.0
74-75	34.06999078263463	35.5	34.0	39.0	28.0	40.5
76-77	33.75582921866574	35.0	34.0	37.5	28.0	40.0
78-79	33.40301687045586	35.0	34.0	37.0	28.0	39.0
80-81	33.09635780205995	35.0	34.0	37.0	27.0	39.0
82-83	32.79656371073536	35.0	34.0	36.0	27.0	38.5
84-85	32.64642607175776	35.0	34.0	36.0	27.0	37.0
86-87	32.431462816918646	35.0	34.0	36.0	27.0	37.0
88-89	32.21015927683841	35.0	34.0	35.0	27.0	36.5
90-91	32.01813808263502	35.0	33.0	35.0	26.5	36.0
92-93	31.832288172705127	35.0	33.0	35.0	26.0	36.0
94-95	31.658733023410576	35.0	33.0	35.0	25.0	36.0
96-97	31.490111889636392	35.0	33.0	35.0	25.0	36.0
98-99	31.310286732730827	35.0	33.0	35.0	25.0	35.0
100	31.18638679277756	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.44699614229870477
1101	2	0.544014059042432
1101	3	0.5118066832432682
1101	4	0.5552907971010868
1101	5	0.5035607397866215
1101	6	0.48985323300991723
1101	7	0.4238615302695621
1101	8	0.5285542150117948
1101	9	0.5759542368749422
1101	10-11	0.5763597025742939
1101	12-13	0.3856092258620123
1101	14-15	0.45480911120603196
1101	16-17	0.5106151906338212
1101	18-19	0.5098389188443662
1101	20-21	0.4484061823553773
1101	22-23	0.3965347757706823
1101	24-25	0.48985923205505344
1101	26-27	0.504673237389575
1101	28-29	0.494055862771674
1101	30-31	0.5326137678536398
1101	32-33	0.49988466399177156
1101	34-35	0.5330730652209326
1101	36-37	0.558915407464859
1101	38-39	0.5302092991418661
1101	40-41	0.5567560379569088
1101	42-43	0.5958186183149934
1101	44-45	0.5480821706990113
1101	46-47	0.5455446264130046
1101	48-49	0.5604399328100271
1101	50-51	0.5999018763789508
1101	52-53	0.5421349079005324
1101	54-55	0.5444790725623463
1101	56-57	0.544109876779288
1101	58-59	0.575708387845637
1101	60-61	0.6080858583464774
1101	62-63	0.6829693528630756
1101	64-65	0.5980109499220276
1101	66-67	0.5806223442309033
1101	68-69	0.6132377480834705
1101	70-71	0.5637510071022547
1101	72-73	0.5560386580974992
1101	74-75	0.5254856349452766
1101	76-77	0.49549390113144653
1101	78-79	0.550923877315185
1101	80-81	0.5501969204555479
1101	82-83	0.5478611603421442
1101	84-85	0.4231151490960059
1101	86-87	0.47302939765821606
1101	88-89	0.4959480195802968
1101	90-91	0.5415929712836203
1101	92-93	0.5500520006648983
1101	94-95	0.583539040236559
1101	96-97	0.525568990984258
1101	98-99	0.4645012553126726
1101	100	0.5765356230243448
1102	1	0.3601684107466596
1102	2	0.42014881779757474
1102	3	0.38870264738408977
1102	4	0.38938667276075023
1102	5	0.31717018782460116
1102	6	0.26803338940766963
1102	7	0.324915647195958
1102	8	0.3125951545650594
1102	9	0.3860858161421561
1102	10-11	0.41438381369107447
1102	12-13	0.4393174738134462
1102	14-15	0.5660960502682784
1102	16-17	0.5150607613494671
1102	18-19	0.48486182914536613
1102	20-21	0.49745336906764237
1102	22-23	0.41178507536692877
1102	24-25	0.4632053757105439
1102	26-27	0.5234626011759858
1102	28-29	0.5185904251180773
1102	30-31	0.5010902267597359
1102	32-33	0.5286006297369283
1102	34-35	0.5929889716545347
1102	36-37	0.5528836174471863
1102	38-39	0.6605329360093393
1102	40-41	0.5934751734667785
1102	42-43	0.5432739948825542
1102	44-45	0.5289338991201831
1102	46-47	0.5003816776567263
1102	48-49	0.539313414470854
1102	50-51	0.5979453245190172
1102	52-53	0.5457147487802914
1102	54-55	0.5140050298726138
1102	56-57	0.5997223535955243
1102	58-59	0.5495748444910831
1102	60-61	0.6134080367332473
1102	62-63	0.5956147451579241
1102	64-65	0.591697490819044
1102	66-67	0.565954464594455
1102	68-69	0.5859935109053467
1102	70-71	0.6201782276998955
1102	72-73	0.5886343040464439
1102	74-75	0.5980936050561496
1102	76-77	0.5166032215523657
1102	78-79	0.5347405146906965
1102	80-81	0.5791840420709562
1102	82-83	0.5466983866035449
1102	84-85	0.5238226337758789
1102	86-87	0.5469602097645776
1102	88-89	0.5691841087129106
1102	90-91	0.5134024232108771
1102	92-93	0.5681632924655631
1102	94-95	0.5833241574133297
1102	96-97	0.48024913401259184
1102	98-99	0.5637617448274135
1102	100	0.580568929861947
1103	1	0.14476171393602755
1103	2	0.3105036650122166
1103	3	0.3047690430652388
1103	4	0.33475390607194555
1103	5	0.26274362109265326
1103	6	0.16098565112158525
1103	7	0.21505125274707382
1103	8	0.2442210112642016
1103	9	0.33046187974620267
1103	10-11	0.4128736193061968
1103	12-13	0.33526456960161255
1103	14-15	0.46863925356521463
1103	16-17	0.4877983072245513
1103	18-19	0.4155733564631561
1103	20-21	0.34350712486768487
1103	22-23	0.2818390962308328
1103	24-25	0.3366313827065426
1103	26-27	0.3884125562702181
1103	28-29	0.40271027910735313
1103	30-31	0.44867296405183055
1103	32-33	0.4678581759503899
1103	34-35	0.37197768952082555
1103	36-37	0.42053073847281297
1103	38-39	0.3937535466995925
1103	40-41	0.44302269674093253
1103	42-43	0.4880404284904003
1103	44-45	0.457257736681143
1103	46-47	0.41718087626147593
1103	48-49	0.4660681937276365
1103	50-51	0.49305968971218306
1103	52-53	0.49845089884028226
1103	54-55	0.48134454286004313
1103	56-57	0.4681269510438568
1103	58-59	0.3952103844811887
1103	60-61	0.5010803458766873
1103	62-63	0.5254105331801284
1103	64-65	0.5317604505392026
1103	66-67	0.4453889973418299
1103	68-69	0.5233385084591404
1103	70-71	0.6289752113293829
1103	72-73	0.6261554703838996
1103	74-75	0.6564726901064049
1103	76-77	0.6370141269569913
1103	78-79	0.5619661348062266
1103	80-81	0.47237369809418084
1103	82-83	0.5234971904323018
1103	84-85	0.5459931826413893
1103	86-87	0.533491475317696
1103	88-89	0.5702699189130769
1103	90-91	0.5375650046510501
1103	92-93	0.5064326650396325
1103	94-95	0.43800147739195694
1103	96-97	0.47262784261575064
1103	98-99	0.49555591003834465
1103	100	0.500134380195604
1104	1	-0.0948032344877241
1104	2	-0.06077494304365416
1104	3	-0.061748136501464046
1104	4	-0.09304187144787335
1104	5	-0.018475323459043125
1104	6	-0.02608167734289424
1104	7	-0.10348154433201984
1104	8	-0.041708848001057675
1104	9	-0.038010182936673687
1104	10-11	-0.06960348869259292
1104	12-13	-0.0199850071771408
1104	14-15	-0.02385305234486168
1104	16-17	-0.03622284525060593
1104	18-19	-0.06928261360843635
1104	20-21	-0.08488151599857474
1104	22-23	-0.022179746080986718
1104	24-25	-0.016551843891186024
1104	26-27	0.04344253282845756
1104	28-29	0.05159559162093785
1104	30-31	0.05512586315527557
1104	32-33	0.08394708288953012
1104	34-35	0.04169319930416293
1104	36-37	0.02399355881855314
1104	38-39	0.09971892051668618
1104	40-41	0.011221850332113092
1104	42-43	0.006315302231215014
1104	44-45	-0.0702505501037507
1104	46-47	-0.03055942366238895
1104	48-49	0.05562288785493763
1104	50-51	0.07514019722071907
1104	52-53	0.057765352818357485
1104	54-55	-0.060314167886964754
1104	56-57	-0.08192395000403963
1104	58-59	-0.023643781238348538
1104	60-61	0.03222461423010259
1104	62-63	0.01717375692887657
1104	64-65	0.012580875077176756
1104	66-67	0.039261609008427456
1104	68-69	0.043227128951507154
1104	70-71	0.025637496767913603
1104	72-73	0.07208939769187594
1104	74-75	0.059061251699702666
1104	76-77	0.03657287228909922
1104	78-79	0.007031561932194563
1104	80-81	-0.03128114195057918
1104	82-83	0.029613903521131135
1104	84-85	0.04618328281694062
1104	86-87	0.04612008759988129
1104	88-89	0.02188041594505563
1104	90-91	0.05211229851022381
1104	92-93	0.041612142168172994
1104	94-95	0.031383600297360204
1104	96-97	0.05381594008848012
1104	98-99	0.11616579989474118
1104	100	0.10855529599688651
1105	1	0.17262245776756302
1105	2	0.20243183572713264
1105	3	0.2936917127593013
1105	4	0.23450614714603546
1105	5	0.2962945979126843
1105	6	0.23734116784407888
1105	7	0.27107488544174174
1105	8	0.27767391477888737
1105	9	0.3738984476163978
1105	10-11	0.3523896324975482
1105	12-13	0.361499674099214
1105	14-15	0.42140011617442497
1105	16-17	0.4360354147951213
1105	18-19	0.3372306170082666
1105	20-21	0.3592047975584265
1105	22-23	0.36776716193796943
1105	24-25	0.3804775799884226
1105	26-27	0.39076383899971034
1105	28-29	0.383626105464117
1105	30-31	0.383297199649661
1105	32-33	0.4646564292549158
1105	34-35	0.4212278249907655
1105	36-37	0.40913194566604716
1105	38-39	0.39243401495504315
1105	40-41	0.39370371182936736
1105	42-43	0.40727854750384296
1105	44-45	0.3617090759761439
1105	46-47	0.3728373779767722
1105	48-49	0.3182355642436221
1105	50-51	0.2685926785011219
1105	52-53	0.3742683002146947
1105	54-55	0.3397965300332402
1105	56-57	0.3409323131770634
1105	58-59	0.33535297712330703
1105	60-61	0.33836038677028313
1105	62-63	0.3146792332333277
1105	64-65	0.2515679891072722
1105	66-67	0.22558839324989322
1105	68-69	0.22667666620658622
1105	70-71	0.21384081622868223
1105	72-73	0.2516635445907198
1105	74-75	0.3204611630257901
1105	76-77	0.2821190307213257
1105	78-79	0.18152541912866837
1105	80-81	0.2124700278740974
1105	82-83	0.16758916536119983
1105	84-85	0.1526573132710709
1105	86-87	0.11381549366830512
1105	88-89	0.00905929806943817
1105	90-91	0.14099023960596213
1105	92-93	0.1647398207255577
1105	94-95	0.1429860779017389
1105	96-97	0.13048227607400165
1105	98-99	0.1730066777343815
1105	100	0.1495064633815808
1106	1	0.09602783890639088
1106	2	0.060742227714271735
1106	3	0.11480681321688024
1106	4	0.10019328863867827
1106	5	0.06464720614323483
1106	6	0.1143227428963911
1106	7	0.13046927556649734
1106	8	0.15623633346861254
1106	9	0.1669075085367382
1106	10-11	0.07436489021662851
1106	12-13	0.06624428822108541
1106	14-15	0.0931041399980117
1106	16-17	0.1057304433194659
1106	18-19	0.1365787286308091
1106	20-21	0.07812351800939865
1106	22-23	0.14053508092612077
1106	24-25	0.17489390110269198
1106	26-27	0.2574699304088668
1106	28-29	0.22861697579785556
1106	30-31	0.20821743876046384
1106	32-33	0.1692993306659787
1106	34-35	0.13193297062274212
1106	36-37	0.1666143659806707
1106	38-39	0.14383335895232818
1106	40-41	0.12490826530900279
1106	42-43	0.1510136069846908
1106	44-45	0.1641740337859403
1106	46-47	0.17707311606314846
1106	48-49	0.17039449855690236
1106	50-51	0.1979113022911676
1106	52-53	0.19832645509507785
1106	54-55	0.1924766313188968
1106	56-57	0.20305008326779728
1106	58-59	0.23141253400378758
1106	60-61	0.21353712106834877
1106	62-63	0.1937662041111068
1106	64-65	0.1645772166843784
1106	66-67	0.10265032811417996
1106	68-69	0.14936391290131468
1106	70-71	0.1512223987805612
1106	72-73	0.17203134566918976
1106	74-75	0.1857394225457938
1106	76-77	0.13021211269458632
1106	78-79	0.19634287267570016
1106	80-81	0.18233176525010464
1106	82-83	0.1314957875721774
1106	84-85	0.17746078848139746
1106	86-87	0.23298763657231092
1106	88-89	0.11073336533835487
1106	90-91	0.1318223464496242
1106	92-93	0.21843902816844718
1106	94-95	0.20583375257105274
1106	96-97	0.2009248643786563
1106	98-99	0.16597730343681505
1106	100	0.20595373682471774
1107	1	0.0766363763679827
1107	2	0.0436015690343865
1107	3	0.040476270669703496
1107	4	0.02251023565790433
1107	5	0.10712915435996706
1107	6	0.0419999357358094
1107	7	0.026876244548510897
1107	8	0.06544185117387968
1107	9	0.0023909192409234947
1107	10-11	-0.007476315239927089
1107	12-13	0.05668869076693284
1107	14-15	0.10130043944613476
1107	16-17	0.0314065252799125
1107	18-19	0.0835013900522199
1107	20-21	0.05766729326533948
1107	22-23	-0.003996642821725516
1107	24-25	0.026948517620134282
1107	26-27	0.004896054409456951
1107	28-29	0.011665370373911799
1107	30-31	0.022966904978027003
1107	32-33	0.07289567393777219
1107	34-35	0.08063558628410306
1107	36-37	0.12807196148823863
1107	38-39	0.07005316011518659
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2216	50-51	-0.33883626616163554
2216	52-53	-0.48044061233583335
2216	54-55	-0.4295359441572586
2216	56-57	-0.44289467517521786
2216	58-59	-0.38237547036334263
2216	60-61	-0.42565614116594475
2216	62-63	-0.48957528430867825
2216	64-65	-0.4404451515527228
2216	66-67	-0.48421498980104616
2216	68-69	-0.5010322745125606
2216	70-71	-0.5916381558984654
2216	72-73	-0.527252421381597
2216	74-75	-0.48399405591479194
2216	76-77	-0.5168785186089693
2216	78-79	-0.4458495474210977
2216	80-81	-0.4521623364588052
2216	82-83	-0.5341623944759775
2216	84-85	-0.5496408937964361
2216	86-87	-0.5017224917121865
2216	88-89	-0.41571640931496745
2216	90-91	-0.4316134913274041
2216	92-93	-0.5044470569240573
2216	94-95	-0.49362027271304143
2216	96-97	-0.5047807836012126
2216	98-99	-0.47986792966551306
2216	100	-0.4608528977541795
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28481.0
3	4685.0
4	3148.0
5	2793.0
6	2050.0
7	2367.0
8	2774.0
9	2804.0
10	3057.0
11	3259.0
12	3624.0
13	4095.0
14	4625.0
15	4793.0
16	4901.0
17	4735.0
18	4612.0
19	3998.0
20	3921.0
21	4275.0
22	5185.0
23	8045.0
24	13267.0
25	17780.0
26	16970.0
27	13469.0
28	14414.0
29	18194.0
30	21735.0
31	25969.0
32	33971.0
33	46107.0
34	67299.0
35	107198.0
36	207485.0
37	424744.0
38	658284.0
39	291972.0
40	619.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.088324676865604	19.316442057802494	13.191959245069256	23.40327402026264
2	15.700563179603627	20.03993116108965	40.089670479408376	24.16983517989835
3	18.548095780569923	25.67905652875575	29.302334037775786	26.470513652898543
4	12.485299225942644	16.64591732351655	38.352824318931916	32.51595913160889
5	13.171000574622177	38.43983141226438	34.600149564440215	13.789018448673227
6	30.91308383491383	37.63437413280001	17.827382945944137	13.62515908634202
7	28.009092466228598	32.810530878692795	21.620730669897164	17.559645985181444
8	25.76093868042561	36.087083622994875	20.038295129906324	18.11368256667319
9	25.60479481974213	17.450596601388746	20.50242836549066	36.44218021337847
10-11	24.198702115479524	27.294448789013504	27.89781892532559	20.60903017018138
12-13	24.805842348173083	24.75960095085518	27.953582536109796	22.480974164861944
14-15	22.17952195798294	26.42737295056673	25.230497700883813	26.162607390566517
16-17	22.00580524720861	29.759690323025122	25.659465366107575	22.575039063658693
18-19	22.000881260020027	28.64118986369175	27.62776476194258	21.73016411434564
20-21	23.214250686895664	27.81402362739277	27.116004633047254	21.85572105266431
22-23	22.93400084108273	27.614473129607596	27.094848583579367	22.35667744573031
24-25	22.58050548943199	27.665923897897404	27.17713181215246	22.576438800518144
26-27	22.33591606404279	27.686330983439106	27.328679793222065	22.649073159296037
28-29	22.452391944484976	27.729141799167085	27.288380070278844	22.5300861860691
30-31	22.552195828639654	28.14394391554415	26.909883985879368	22.393976269936825
32-33	22.28926081459784	27.830167127354322	27.437639716339202	22.442932341708637
34-35	22.55843826546234	27.5318623698714	27.336765156154318	22.572934208511942
36-37	22.751742805366483	27.958894095989685	26.836858491667257	22.45250460697658
38-39	22.56587623240982	28.045565525319883	27.08549563942745	22.303062602842846
40-41	22.34235626394745	27.738341311393604	27.34610827660263	22.57319414805631
42-43	22.371082723314675	27.68118750299029	27.318549351705663	22.62918042198938
44-45	22.410991700819967	27.82050595047285	27.268782241496282	22.499720107210898
46-47	22.483394317525292	27.941182451385092	27.10815314189201	22.467270089197605
48-49	22.330148186940285	27.76029606287006	27.236362966494838	22.67319278369482
50-51	22.328604963400828	27.74905284271591	27.243702923244612	22.67863927063865
52-53	22.43820980078328	27.6052843748729	27.299485390693967	22.657020433649848
54-55	22.436564399506302	28.030711010321134	27.102595799054946	22.430128791117614
56-57	22.316199752198525	27.786444795271915	27.173902901918417	22.723452550611142
58-59	22.289856459330142	27.370526315789473	27.451363636363634	22.888253588516747
60-61	22.573596395872247	27.77017561319166	27.05923500321524	22.596992987720856
62-63	22.24087960565112	27.817744024899195	27.198852646675704	22.74252372277398
64-65	22.31234512908083	27.529404841135154	27.34024641551123	22.81800361427279
66-67	22.158725814654815	27.664604362464758	27.242845530050136	22.93382429283029
68-69	22.633115286201214	27.770986398528656	27.0632821515855	22.532616163684626
70-71	22.502411349417926	27.540672383733728	27.215140212313578	22.741776054534775
72-73	22.29962614550949	27.655376395016933	27.338647865814274	22.706349593659304
74-75	22.307357951779476	27.767266718067063	27.202851119298284	22.72252421085518
76-77	22.391757618208295	27.73354595229757	27.039687597482253	22.83500883201188
78-79	22.426960110041268	27.75374678547934	27.181292984869327	22.638000119610073
80-81	22.376335587141618	27.683087634419923	27.18810942293449	22.75246735550397
82-83	22.298337429217124	27.677728383697193	27.321406827833894	22.70252735925179
84-85	22.29887922622569	27.694625926711815	27.18044455374181	22.82605029332069
86-87	22.525322286363057	27.708027178532372	27.119229148959228	22.647421386145343
88-89	22.28266381905413	27.68061671479668	27.11949064478904	22.917228821360155
90-91	22.27518557172789	27.601634174620536	27.231052858071326	22.892127395580246
92-93	22.50929323567263	27.707907742210185	27.078144510412727	22.704654511704454
94-95	22.21668853033117	27.53228598247271	27.225453132819133	23.02557235437699
96-97	22.379073010089517	27.5057128648238	27.20388127957647	22.911332845510213
98-99	22.311985498392296	27.599986795844135	27.113849006650398	22.974178699113168
100	22.405101745472464	27.556123266667143	27.028630341128995	23.010144646731394
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2685.0
1	1833.5
2	867.5
3	655.0
4	494.5
5	396.0
6	333.0
7	317.0
8	332.5
9	324.5
10	308.0
11	297.5
12	306.0
13	339.0
14	378.0
15	402.0
16	472.5
17	606.0
18	835.5
19	1123.5
20	1520.5
21	2205.5
22	3132.5
23	4604.5
24	6517.5
25	8692.5
26	11695.0
27	15921.0
28	21081.0
29	26137.5
30	30802.5
31	36030.0
32	41207.0
33	46078.0
34	50633.0
35	54568.5
36	58548.5
37	62075.0
38	65109.0
39	66442.0
40	67668.0
41	69759.5
42	70119.0
43	70473.0
44	70532.0
45	69663.5
46	70005.0
47	69468.0
48	68299.5
49	67701.5
50	66857.0
51	65045.5
52	61331.5
53	58689.5
54	57232.0
55	54940.5
56	51522.5
57	47279.5
58	43283.0
59	40242.0
60	36711.0
61	32526.5
62	28805.5
63	25069.0
64	21160.0
65	17433.5
66	14430.0
67	11640.5
68	8897.0
69	6778.0
70	5223.0
71	4061.0
72	3256.5
73	2639.0
74	2152.5
75	1582.5
76	1149.0
77	916.0
78	673.0
79	466.5
80	324.5
81	212.0
82	145.5
83	97.0
84	66.0
85	44.5
86	33.5
87	27.5
88	22.0
89	16.0
90	14.0
91	14.0
92	8.5
93	7.0
94	6.0
95	4.0
96	3.5
97	2.0
98	1.5
99	1.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07419787885857655
2	0.076827313998539
3	0.07816593552433805
4	0.08022167572467233
5	0.07816593552433805
6	0.07859620672905918
7	0.0775444326730742
8	0.07749662476143852
9	0.07720977729162444
10-11	0.07699464168926387
12-13	0.07579944389837186
14-15	0.07424568677021222
16-17	0.07295487315604886
18-19	0.07242898612805636
20-21	0.07185529118842819
22-23	0.07367199183058407
24-25	0.0741739749027587
26-27	0.0735763760073127
28-29	0.06975174307645823
30-31	0.07508232522383665
32-33	0.0732178166700451
34-35	0.06999078263463664
36-37	0.06673984464341035
38-39	0.06881948879956247
40-41	0.07135330811625354
42-43	0.07668389026363194
44-45	0.07735320102653148
46-47	0.08038900341539722
48-49	0.08330528602517374
50-51	0.08442877194861223
52-53	0.08457219568351927
54-55	0.0845960996393371
56-57	0.08244474361573148
58-59	0.08146468142720002
60-61	0.07916990166868734
62-63	0.08014996385721881
64-65	0.07921770958032302
66-67	0.07873963046396622
68-69	0.07821374343597373
70-71	0.07591896367746105
72-73	0.07582334785418969
74-75	0.0754169806052864
76-77	0.07553650038437561
78-79	0.07548869247273993
80-81	0.0770663535567174
82-83	0.07637313883800002
84-85	0.07579944389837186
86-87	0.0764209467496357
88-89	0.07324172062586293
90-91	0.07049276570681129
92-93	0.07046886175099344
94-95	0.07066009339753618
96-97	0.07006249450209016
98-99	0.06946489560664415
100	0.06879558484374462
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2091704.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.24699140434677
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.05235878261455	51.579285739825885
2	12.920106372501433	16.85996138859694
3	3.7765336800096763	7.392223816734522
4	1.4975444586526616	3.908410816853495
5	0.7633897257897146	2.4904441438384084
6	0.45239273343285263	1.7710358873809366
7	0.3052339735490856	1.394091891393026
8	0.21763940575294194	1.1360253149127475
9	0.15994881513784084	0.9392561069790738
>10	0.7988381515260949	9.20559537875584
>50	0.04382040386660158	1.9427217036765025
>100	0.01204679451613551	1.235071954810085
>500	0.0	0.0
>1k	1.467026505218475E-4	0.14587585624253063
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.780791163568077E-5	0.0
8	0.0	0.0	0.0	4.780791163568077E-5	0.0
9	0.0	0.0	0.0	1.9123164654272307E-4	0.0
10-11	0.0	0.0	0.0	2.390395581784038E-4	0.0
12-13	0.0	0.0	0.0	3.1075142563192495E-4	0.0
14-15	0.0	0.0	0.0	3.8246329308544614E-4	0.0
16-17	0.0	0.0	0.0	4.063672489032865E-4	0.0
18-19	0.0	0.0	0.0	4.7807911635680767E-4	0.0
20-21	0.0	2.3903955817840384E-5	0.0	5.01983072174648E-4	0.0
22-23	0.0	4.780791163568077E-5	0.0	7.171186745352115E-4	0.0
24-25	0.0	9.561582327136152E-5	0.0	9.32254276895775E-4	0.0
26-27	0.0	1.4342373490704228E-4	0.0	0.001123485923438498	0.0
28-29	0.0	1.4342373490704228E-4	0.0	0.002007932288698592	0.0
30-31	0.0	1.4342373490704228E-4	0.0	0.006215028512638499	0.0
32-33	0.0	1.4342373490704228E-4	0.0	0.01608736226540658	0.0
34-35	0.0	1.4342373490704228E-4	0.0	0.029640905214122076	0.0
36-37	0.0	1.4342373490704228E-4	0.0	0.05428588366231551	0.0
38-39	0.0	1.6732769072488266E-4	0.0	0.09738471600188173	0.0
40-41	0.0	1.9123164654272307E-4	0.0	0.15202915900146485	0.0
42-43	0.0	1.9123164654272307E-4	0.0	0.20820345517338973	0.0
44-45	0.0	1.9123164654272307E-4	0.0	0.2728397517048301	0.0
46-47	0.0	1.9123164654272307E-4	0.0	0.34808940461939164	0.0
48-49	0.0	1.9123164654272307E-4	0.0	0.4378726626712001	0.0
50-51	0.0	1.9123164654272307E-4	0.0	0.5384366047968546	0.0
52-53	0.0	1.9123164654272307E-4	0.0	0.636036456401097	0.0
54-55	0.0	1.9123164654272307E-4	0.0	0.7387995624619927	0.0
56-57	0.0	1.9123164654272307E-4	0.0	0.86379334743348	0.0
58-59	0.0	1.9123164654272307E-4	0.0	1.0081254326616003	0.0
60-61	0.0	1.9123164654272307E-4	0.0	1.1720826656161676	0.0
62-63	0.0	1.9123164654272307E-4	0.0	1.3342710058402145	0.0
64-65	0.0	2.3903955817840383E-4	0.0	1.5019333519465468	0.0
66-67	0.0	2.3903955817840383E-4	0.0	1.6899857723654974	0.0
68-69	0.0	2.3903955817840383E-4	0.0	1.9050257589027892	0.0
70-71	0.0	2.3903955817840383E-4	0.0	2.1408382830457846	0.0
72-73	0.0	2.3903955817840383E-4	0.0	2.3942441186707106	0.0
74-75	0.0	2.3903955817840383E-4	0.0	2.645904965520934	0.0
76-77	0.0	2.3903955817840383E-4	0.0	2.905764869216677	0.0
78-79	0.0	2.3903955817840383E-4	0.0	3.2043252773815034	0.0
80-81	0.0	2.3903955817840383E-4	0.0	3.5388850430079977	0.0
82-83	0.0	2.3903955817840383E-4	0.0	3.893356803830752	0.0
84-85	0.0	2.3903955817840383E-4	0.0	4.231071891625201	0.0
86-87	0.0	2.3903955817840383E-4	0.0	4.577464115381526	0.0
88	0.0	2.3903955817840383E-4	0.0	4.8571882063618945	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2180	0.0	43.9809	1
GTATCAA	3675	0.0	39.517693	1
TCAACGC	3955	0.0	34.938293	4
ATCAACG	3985	0.0	34.67527	3
CAACGCA	4135	0.0	33.4174	5
AACGCAG	4250	0.0	32.513165	6
TATCAAC	4585	0.0	30.751928	2
GTACATG	8810	0.0	26.780474	1
TACATGG	8755	0.0	25.71401	2
ACGCAGA	5455	0.0	25.244904	7
CGCAGAG	5570	0.0	24.723688	8
ACATGGG	8860	0.0	24.29588	3
GCAGAGT	6375	0.0	21.453753	9
GAGTACA	6635	0.0	20.542227	1
CATGGGG	5855	0.0	19.907907	4
TACCGTC	215	1.1948741E-6	19.674555	7
AGTACAT	6535	0.0	19.490105	2
CATGGGA	2695	0.0	18.311813	4
TATAACG	160	0.0012792151	17.6247	2
CAGAGTA	6105	0.0	17.591017	10-11
>>END_MODULE