FastQCFastQC Report
Wed 25 May 2016
SRR1032924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032924_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091704
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71870.34359546092563764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56930.2721704409419306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31870.1523638143829146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40600.058.6657451
GTATCAA79550.044.705231
ATCAACG98100.036.105123
TCAACGC99050.035.7588354
CAACGCA100900.035.0540585
TATCAAC102300.034.714762
AACGCAG103300.034.421776
ACGCAGA120400.029.37677
CGCAGAG123400.028.510068
GTGGTAT15600.028.006641
GCAGAGT132650.026.311959
GTACATG86400.026.0993791
TACATGG85600.025.1671812
TGGTATC17750.024.6449222
ACATGGG88000.023.8928383
GAGTACT86700.022.7021212-13
CAGAGTA129350.022.33470310-11
AGAGTAC124300.021.12575110-11
GTACTTT93300.020.74374214-15
TAGCGCG1605.3825577E-520.578844
Produced by FastQC (version 0.11.5)