FastQCFastQC Report
Wed 25 May 2016
SRR1032923_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032923_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4183703
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33100.035.073941
GTATCAA56050.034.9684221
TCAACGC54450.034.4434
ATCAACG55050.033.981813
CAACGCA57350.032.6189845
AACGCAG60450.031.1009836
GTACATG145800.029.4648531
TATCAAC65200.029.2680662
TACATGG146400.027.9635752
ACATGGG152050.026.1519873
ACGCAGA75300.024.9672227
CGCAGAG78700.023.88838
CATGGGG108300.022.5250034
GAGTACA99650.021.0364841
GCGGGTA2501.0741132E-820.679619
AGTACAT102450.019.6357462
GCAGAGT96500.018.9944579
GTATAGG19400.018.8976781
GTACTAG9450.017.4081171
CATGGGA56350.017.0995984
Produced by FastQC (version 0.11.5)