##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032923_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4183703
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.896405170252287	34.0	31.0	34.0	30.0	34.0
2	32.00725314392537	34.0	31.0	34.0	30.0	34.0
3	32.08343637203693	34.0	31.0	34.0	30.0	34.0
4	35.42146705920568	37.0	35.0	37.0	35.0	37.0
5	35.48662297491003	37.0	37.0	37.0	35.0	37.0
6	35.524864456200646	37.0	37.0	37.0	35.0	37.0
7	35.49128391762035	37.0	37.0	37.0	35.0	37.0
8	35.49429679879284	37.0	37.0	37.0	35.0	37.0
9	37.17855641282376	39.0	38.0	39.0	35.0	39.0
10-11	37.17849163767122	39.0	38.5	39.0	35.0	39.0
12-13	37.13435980039692	39.0	38.0	39.0	35.0	39.0
14-15	38.64931353874785	41.0	39.5	41.0	36.0	41.0
16-17	38.671225825542585	41.0	40.0	41.0	36.0	41.0
18-19	38.677254097625955	41.0	39.5	41.0	36.0	41.0
20-21	38.716040431168274	41.0	40.0	41.0	35.5	41.0
22-23	38.702052464049196	41.0	39.5	41.0	35.0	41.0
24-25	38.727020417080276	41.0	39.0	41.0	35.0	41.0
26-27	38.703814778439096	41.0	39.0	41.0	35.0	41.0
28-29	38.68704470656736	41.0	39.0	41.0	35.0	41.0
30-31	38.64443412928691	41.0	39.0	41.0	35.0	41.0
32-33	38.60289042984169	41.0	39.0	41.0	35.0	41.0
34-35	38.540312493501574	41.0	39.0	41.0	34.5	41.0
36-37	38.4598898392166	41.0	39.0	41.0	34.0	41.0
38-39	38.375394357582266	41.0	39.0	41.0	34.0	41.0
40-41	38.29754143637825	41.0	39.0	41.0	34.0	41.0
42-43	38.191172389627084	41.0	38.5	41.0	34.0	41.0
44-45	38.062487944292414	40.0	38.0	41.0	33.0	41.0
46-47	37.94854187785319	40.0	38.0	41.0	33.0	41.0
48-49	37.82631522839934	40.0	38.0	41.0	33.0	41.0
50-51	37.691847867786024	40.0	38.0	41.0	33.0	41.0
52-53	37.54881656274358	40.0	37.0	41.0	33.0	41.0
54-55	37.39271334509166	40.0	37.0	41.0	32.0	41.0
56-57	37.22061652081899	40.0	36.5	41.0	32.0	41.0
58-59	37.02013037254318	40.0	36.0	41.0	31.5	41.0
60-61	36.784030319551846	39.0	35.5	41.0	31.0	41.0
62-63	36.538163081844004	39.0	35.0	41.0	31.0	41.0
64-65	36.26298580468068	39.0	35.0	41.0	31.0	41.0
66-67	35.96472586605694	38.0	35.0	40.0	31.0	41.0
68-69	35.64983663993357	37.5	35.0	40.0	30.0	41.0
70-71	35.3056509986488	37.0	35.0	40.0	30.0	41.0
72-73	35.006357645368226	36.5	35.0	39.0	30.0	41.0
74-75	34.716757618788904	36.0	35.0	39.0	30.0	41.0
76-77	34.38793050080276	35.5	34.0	38.5	29.5	40.0
78-79	34.01674234523818	35.0	34.0	37.0	29.0	39.0
80-81	33.69496950428842	35.0	34.0	37.0	29.0	39.0
82-83	33.38007932207425	35.0	34.0	36.5	29.0	39.0
84-85	33.21099824724652	35.0	34.0	36.0	29.0	37.0
86-87	32.980454396499944	35.0	34.0	36.0	29.0	37.0
88-89	32.75369833852929	35.0	34.0	35.5	29.0	37.0
90-91	32.56372918918957	35.0	34.0	35.0	29.0	36.0
92-93	32.386624480753056	35.0	34.0	35.0	28.0	36.0
94-95	32.21835799529747	35.0	34.0	35.0	28.0	36.0
96-97	32.06045362206638	35.0	34.0	35.0	27.0	36.0
98-99	31.89542386254474	35.0	34.0	35.0	27.0	35.5
100	31.779322289369013	35.0	34.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23405692949264534
1101	2	0.29806843425386376
1101	3	0.28941840693376264
1101	4	0.24571487197157893
1101	5	0.1803276469519659
1101	6	0.15917398150482853
1101	7	0.2777365329532486
1101	8	0.1805095596461328
1101	9	0.282746487769181
1101	10-11	0.25179176343426946
1101	12-13	0.08054321087155358
1101	14-15	0.13351137722197848
1101	16-17	0.18261745657508754
1101	18-19	0.15809699538619526
1101	20-21	0.11155647027163695
1101	22-23	0.12938329492521916
1101	24-25	0.1335185270075172
1101	26-27	0.17037501245197717
1101	28-29	0.19466273246783317
1101	30-31	0.22109169357266012
1101	32-33	0.24354807340546358
1101	34-35	0.2150919794208903
1101	36-37	0.1949710672693925
1101	38-39	0.16065029969165323
1101	40-41	0.17310550259383461
1101	42-43	0.19921801026747943
1101	44-45	0.1959477294964458
1101	46-47	0.2418304812064065
1101	48-49	0.23059288641819364
1101	50-51	0.27226084958109453
1101	52-53	0.2841982888509236
1101	54-55	0.2951279624601355
1101	56-57	0.28409207335272413
1101	58-59	0.24419996693603707
1101	60-61	0.29180501665284453
1101	62-63	0.27782407701992184
1101	64-65	0.26293917907553066
1101	66-67	0.2728777208178599
1101	68-69	0.25944439471192027
1101	70-71	0.28345092864677923
1101	72-73	0.25056540251400605
1101	74-75	0.22671402406847818
1101	76-77	0.21998948324999645
1101	78-79	0.2416022112216396
1101	80-81	0.22592199073880437
1101	82-83	0.231912130668249
1101	84-85	0.20384817148537593
1101	86-87	0.16767421506762048
1101	88-89	0.1581123423171391
1101	90-91	0.1510110330799037
1101	92-93	0.18172012225757328
1101	94-95	0.210891356588796
1101	96-97	0.21732589634250132
1101	98-99	0.18809728388561098
1101	100	0.19174410792744112
1102	1	0.17051032750242
1102	2	0.17030232093669184
1102	3	0.22165448717525038
1102	4	0.2196662371033753
1102	5	0.17989572720200186
1102	6	0.17411143678308605
1102	7	0.15937385595136533
1102	8	0.16923675603792532
1102	9	0.2402372956389769
1102	10-11	0.2123653136196637
1102	12-13	0.18913178542443632
1102	14-15	0.2552931254303772
1102	16-17	0.22871775201372202
1102	18-19	0.2035465183186247
1102	20-21	0.2139566372537729
1102	22-23	0.23733606448799094
1102	24-25	0.24110440375995523
1102	26-27	0.2486779187536925
1102	28-29	0.2629442264171189
1102	30-31	0.2377977055207623
1102	32-33	0.21446534064894962
1102	34-35	0.23780408453160362
1102	36-37	0.24936478161698972
1102	38-39	0.25851656707362025
1102	40-41	0.23453464127868529
1102	42-43	0.303548074938476
1102	44-45	0.254826604683835
1102	46-47	0.2908997218162668
1102	48-49	0.2523918472700899
1102	50-51	0.2813487731728017
1102	52-53	0.24368688278610762
1102	54-55	0.2739543985146966
1102	56-57	0.27522800217073495
1102	58-59	0.2747903310504185
1102	60-61	0.3453240117514156
1102	62-63	0.3099184563906121
1102	64-65	0.34268742545493325
1102	66-67	0.38339679050245934
1102	68-69	0.3665915911766717
1102	70-71	0.33304667364608775
1102	72-73	0.3125043486323307
1102	74-75	0.28035719295073136
1102	76-77	0.2789537927676591
1102	78-79	0.26606501260484094
1102	80-81	0.2388132041905493
1102	82-83	0.2883248560252696
1102	84-85	0.25121451125226457
1102	86-87	0.2500442294225991
1102	88-89	0.24174009968892562
1102	90-91	0.21979868691672522
1102	92-93	0.24591696219987114
1102	94-95	0.26845192691907727
1102	96-97	0.2590567147474161
1102	98-99	0.1961046848820942
1102	100	0.22246487180908758
1103	1	0.2261831364944129
1103	2	0.26287107985226754
1103	3	0.2822935989515116
1103	4	0.23593921540523866
1103	5	0.15976181304677084
1103	6	0.1771312153622091
1103	7	0.2270987591793343
1103	8	0.2137465572576076
1103	9	0.25440420544642706
1103	10-11	0.25536528546125936
1103	12-13	0.26202908695115923
1103	14-15	0.3184977668930742
1103	16-17	0.3518880701856588
1103	18-19	0.3330035884432263
1103	20-21	0.30932140534539343
1103	22-23	0.30433040704629377
1103	24-25	0.3360317281309264
1103	26-27	0.3606080032468668
1103	28-29	0.37890665352730935
1103	30-31	0.3872907644754662
1103	32-33	0.3884465008828215
1103	34-35	0.3560446159704469
1103	36-37	0.3722243688627884
1103	38-39	0.3359778957066979
1103	40-41	0.3344593025840723
1103	42-43	0.3361807234635421
1103	44-45	0.34380241182636695
1103	46-47	0.3497165658109509
1103	48-49	0.3664325235749146
1103	50-51	0.3740901370817582
1103	52-53	0.3728024873918656
1103	54-55	0.3793892091254918
1103	56-57	0.37662411954691777
1103	58-59	0.3396926843137038
1103	60-61	0.3559959849781791
1103	62-63	0.3558618795771977
1103	64-65	0.32796193090058523
1103	66-67	0.2867750494626691
1103	68-69	0.29764182755744173
1103	70-71	0.3010303345854126
1103	72-73	0.2796619200363395
1103	74-75	0.2859728463843467
1103	76-77	0.2603498682058145
1103	78-79	0.24969629574712826
1103	80-81	0.2302689574606731
1103	82-83	0.2334930844845502
1103	84-85	0.22192690076798982
1103	86-87	0.24182515228715573
1103	88-89	0.2692628675516815
1103	90-91	0.23758245318044402
1103	92-93	0.2675463868878296
1103	94-95	0.22536475390848665
1103	96-97	0.18522886800818839
1103	98-99	0.20464431842749775
1103	100	0.22465164107617142
1104	1	0.184353427072125
1104	2	0.21754730390865262
1104	3	0.2341337664659804
1104	4	0.22046551701568973
1104	5	0.24953485105606887
1104	6	0.24071248468207784
1104	7	0.19538200755873447
1104	8	0.23360889662124862
1104	9	0.23167412886359529
1104	10-11	0.24259444602159164
1104	12-13	0.22173753800306883
1104	14-15	0.3462884269900499
1104	16-17	0.3079502949670001
1104	18-19	0.3287520184286379
1104	20-21	0.3067192250441835
1104	22-23	0.32490836550413604
1104	24-25	0.35466230773123186
1104	26-27	0.38679803759265496
1104	28-29	0.34174805184765233
1104	30-31	0.3118774270259621
1104	32-33	0.3242766531408492
1104	34-35	0.3252130155354749
1104	36-37	0.32436356612761585
1104	38-39	0.30025891487640166
1104	40-41	0.34274521607154185
1104	42-43	0.3410433027447226
1104	44-45	0.3108525418732313
1104	46-47	0.28977137685131993
1104	48-49	0.3382890979802795
1104	50-51	0.3379487568563988
1104	52-53	0.3346858631877083
1104	54-55	0.32861733948570304
1104	56-57	0.2679443366173757
1104	58-59	0.30668474275415747
1104	60-61	0.3540761532788821
1104	62-63	0.36901861047817164
1104	64-65	0.29588943649240207
1104	66-67	0.2990338817522229
1104	68-69	0.2739130869973323
1104	70-71	0.33114062149788737
1104	72-73	0.2912804326516678
1104	74-75	0.31102428412632577
1104	76-77	0.2790512379326131
1104	78-79	0.2537024850414866
1104	80-81	0.27173772106442584
1104	82-83	0.2297912435943914
1104	84-85	0.192823284719303
1104	86-87	0.2004428746198954
1104	88-89	0.2300979482362422
1104	90-91	0.25870308776521966
1104	92-93	0.15124205351460773
1104	94-95	0.19912323846212843
1104	96-97	0.1987546094256416
1104	98-99	0.16687855194298606
1104	100	0.11250493410335949
1105	1	0.0014147873970209446
1105	2	0.01669493009251255
1105	3	0.0457388024234433
1105	4	0.05228504090717223
1105	5	0.09761523854353271
1105	6	0.03060469906212404
1105	7	0.018771421869402616
1105	8	0.06277542649291235
1105	9	0.07175303111657172
1105	10-11	0.07947908820400329
1105	12-13	0.05839653751701945
1105	14-15	0.11701275957689461
1105	16-17	0.12066980949562378
1105	18-19	0.12558797485453965
1105	20-21	0.10110011185828682
1105	22-23	0.1578627352229489
1105	24-25	0.1371052741632326
1105	26-27	0.1591143324035187
1105	28-29	0.13202594523333033
1105	30-31	0.18673835210507406
1105	32-33	0.21345520660249662
1105	34-35	0.22866384776311577
1105	36-37	0.19050083889177216
1105	38-39	0.1703808464815495
1105	40-41	0.17759070072546734
1105	42-43	0.12794255297545476
1105	44-45	0.12490880612823929
1105	46-47	0.09403129487601092
1105	48-49	0.11343528071430597
1105	50-51	0.14119840923127924
1105	52-53	0.12504892873556628
1105	54-55	0.13584921879039058
1105	56-57	0.12292687494236532
1105	58-59	0.10502096729825894
1105	60-61	0.13697220134582722
1105	62-63	0.1515819565138159
1105	64-65	0.12495200216091007
1105	66-67	0.11539062344665751
1105	68-69	0.1342179734917437
1105	70-71	0.1414142413523578
1105	72-73	0.10240812313304559
1105	74-75	0.10064663927091999
1105	76-77	0.04979693264838403
1105	78-79	0.10330701759333749
1105	80-81	0.043370151395969
1105	82-83	0.08039893950998334
1105	84-85	0.013756772722189226
1105	86-87	-0.0028716158234090017
1105	88-89	0.060962477186649267
1105	90-91	0.06768185309054076
1105	92-93	0.06029026095840351
1105	94-95	0.08239512182148445
1105	96-97	0.022707342799854757
1105	98-99	0.01916523381352775
1105	100	0.04673035878414211
1106	1	-0.040464343568949346
1106	2	-0.056967154299503164
1106	3	0.013087438487275449
1106	4	-0.01166804498112839
1106	5	0.028362072107086078
1106	6	-0.005136355682431315
1106	7	0.027062417172501796
1106	8	-0.0025624398320118758
1106	9	0.055512636654768244
1106	10-11	0.09294279700547747
1106	12-13	0.09502816440217998
1106	14-15	0.10016929059758439
1106	16-17	0.01670519221163147
1106	18-19	0.10688606487101282
1106	20-21	0.05113483305814981
1106	22-23	0.07675310960151194
1106	24-25	0.08814524639677046
1106	26-27	0.0748614947832067
1106	28-29	0.03320804844003078
1106	30-31	0.04696685124282851
1106	32-33	0.02683872940267662
1106	34-35	0.03109873783749606
1106	36-37	0.09080239452013927
1106	38-39	0.0769691541365205
1106	40-41	0.06836926334074178
1106	42-43	0.04209221475669267
1106	44-45	-0.009279892663109024
1106	46-47	0.01728802218393355
1106	48-49	0.014348045976163348
1106	50-51	-0.016954701104353376
1106	52-53	-0.032875676689002375
1106	54-55	-0.031560288934841196
1106	56-57	0.02096560869632924
1106	58-59	0.0011359560605157526
1106	60-61	0.05985496262042744
1106	62-63	-0.0131538010105956
1106	64-65	0.05077939987744173
1106	66-67	-0.007568663027043954
1106	68-69	0.04869836710119557
1106	70-71	0.06288237156312704
1106	72-73	0.014233516671907864
1106	74-75	0.036736879435814274
1106	76-77	-0.07748406858599566
1106	78-79	0.008010353269057191
1106	80-81	-0.0016186145337044877
1106	82-83	-0.034997654479653306
1106	84-85	0.016505326626592876
1106	86-87	0.016428841879204015
1106	88-89	-0.0401928614884568
1106	90-91	-0.022485764938572572
1106	92-93	-0.04364773350247475
1106	94-95	-0.046564888625056255
1106	96-97	-0.03709182165550118
1106	98-99	-0.07776896375253983
1106	100	-0.07667890457270587
1107	1	-0.04106152772601135
1107	2	0.027251358637251144
1107	3	0.025580450027348434
1107	4	-0.008358882603751283
1107	5	-0.0208925934495241
1107	6	-0.01701326864673547
1107	7	0.019653636830938126
1107	8	-0.004609501539100336
1107	9	-0.09257019583893822
1107	10-11	-0.059573947445791475
1107	12-13	-0.025259625948066855
1107	14-15	0.006834230405679875
1107	16-17	0.05202634992598121
1107	18-19	0.056229428163568684
1107	20-21	0.06710917376279468
1107	22-23	0.05500090773023203
1107	24-25	0.051039625953194445
1107	26-27	0.06738398192545958
1107	28-29	0.020163682860527388
1107	30-31	0.04088535921675884
1107	32-33	0.019711563739711835
1107	34-35	0.03387779008079406
1107	36-37	0.0454504799859663
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2216	34-35	-0.31796127980695843
2216	36-37	-0.30285373679923566
2216	38-39	-0.28569867599082954
2216	40-41	-0.27593031247055677
2216	42-43	-0.23515793280340347
2216	44-45	-0.21933006116729103
2216	46-47	-0.15499169639766563
2216	48-49	-0.19591105556384747
2216	50-51	-0.1678935257673757
2216	52-53	-0.1642917533945436
2216	54-55	-0.1505361063830577
2216	56-57	-0.1530903961217831
2216	58-59	-0.11237602994658857
2216	60-61	-0.14514785940743735
2216	62-63	-0.121415092864261
2216	64-65	-0.11947313815524296
2216	66-67	-0.16531915758008608
2216	68-69	-0.13882597871570113
2216	70-71	-0.17435221498455178
2216	72-73	-0.17901441023082043
2216	74-75	-0.14744489323964416
2216	76-77	-0.2031795498338056
2216	78-79	-0.13447509194628537
2216	80-81	-0.173253193720889
2216	82-83	-0.17523559197851313
2216	84-85	-0.20532257604217108
2216	86-87	-0.15352706283100304
2216	88-89	-0.13267044759636093
2216	90-91	-0.18045508368994234
2216	92-93	-0.23045972267191672
2216	94-95	-0.2008533646022599
2216	96-97	-0.21408561343792343
2216	98-99	-0.19610282038321003
2216	100	-0.21240056165479615
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45796.0
3	6538.0
4	4371.0
5	3802.0
6	3031.0
7	3600.0
8	4104.0
9	4437.0
10	4637.0
11	5245.0
12	5665.0
13	6344.0
14	7018.0
15	7478.0
16	7524.0
17	7550.0
18	7466.0
19	6875.0
20	6883.0
21	7258.0
22	8504.0
23	11618.0
24	18042.0
25	24567.0
26	25938.0
27	22538.0
28	24919.0
29	32109.0
30	39257.0
31	47570.0
32	62828.0
33	84945.0
34	125294.0
35	198573.0
36	382178.0
37	834900.0
38	1430346.0
39	652897.0
40	1058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.665620606781715	19.217050608678637	13.249844693715914	23.867484090823737
2	15.270850526869758	19.46179147679022	41.14880332986473	24.118554666475294
3	18.544337181945295	25.08026347422849	29.39187306159015	26.983526282236063
4	12.32185981309529	16.047131659810944	38.62683767901335	33.00417084808041
5	12.968068490925633	38.44012294837847	35.68024331320475	12.911565247491144
6	31.578667860843908	37.81745450787554	17.54947749961486	13.054400131665695
7	28.40386755913615	32.17593140797419	21.88495066515386	17.535250367735795
8	25.636075707122902	37.050752095533355	19.644022467179546	17.6691497301642
9	25.404949990802162	17.258202759542247	20.492587787904835	36.84425946175075
10-11	24.426646311058303	27.079972112306216	28.394853670342723	20.098527906292755
12-13	24.785088548672977	24.22131265676638	28.66101192366396	22.332586870896684
14-15	21.88257022767226	26.363092488611844	25.452673750292732	26.301663533423163
16-17	21.902871607984455	29.829093307674505	26.187313830471133	22.080721253869903
18-19	21.848314857368873	28.801012026848806	28.02737648879452	21.323296626987805
20-21	22.719163725060334	28.034530954022912	27.750668035868102	21.495637285048648
22-23	22.56459159149749	27.726524766778006	27.73754030358867	21.97134333813584
24-25	22.27722162413396	27.82636173454553	27.704843701616298	22.19157293970421
26-27	22.017504484841005	27.94296050098655	27.766102459973713	22.27343255419873
28-29	22.11096739316983	27.94484340624677	27.914441380720785	22.029747819862617
30-31	22.255974783416082	28.210287785436094	27.547508045536535	21.986229385611292
32-33	22.015739521786355	28.03434425416796	27.932908909960553	22.017007314085134
34-35	22.21243229893299	27.876089630621426	27.8761135506186	22.035364519826985
36-37	22.312650310119174	28.01661623091557	27.51953814780978	22.151195311155476
38-39	22.111795745169424	28.33189018001942	27.670858189855192	21.885455884955967
40-41	22.029756257884696	27.930009955589746	27.929507631263327	22.11072615526223
42-43	21.99518105144936	27.941074629114986	27.857876301693214	22.20586801774244
44-45	21.981949461506343	28.036284795046118	27.976600347557252	22.005165395890288
46-47	22.031897342502134	28.1281371918009	27.802362642955476	22.037602822741494
48-49	21.983629856970577	28.027494414539163	27.829362765843175	22.15951296264709
50-51	21.993685648954234	27.959579923645972	27.87271393650051	22.174020490899288
52-53	22.029594515307725	28.01048325985841	27.875016671660692	22.08490555317318
54-55	22.038797138468215	28.3116933760032	27.673942027258857	21.975567458269726
56-57	21.943106720561843	28.056851414305843	27.790816440652765	22.20922542447955
58-59	21.910565546463342	27.715845951349884	28.120761200478263	22.25282730170851
60-61	22.114120535029194	28.12360017850733	27.723142834211735	22.039136452251743
62-63	21.86759306483607	28.07199122339484	27.872670807453414	22.18774490431567
64-65	21.939334018143484	27.961042372269112	27.859469111059333	22.24015449852807
66-67	21.79163557803798	27.958367591626633	27.888228296229915	22.361768534105472
68-69	22.010934927837873	28.10332192299812	27.80536302101692	22.080380128147088
70-71	21.99188298795605	27.90630750108364	27.89047152921904	22.21133798174127
72-73	21.817891499904913	28.036295873685667	27.85003223402741	22.295780392382007
74-75	21.94293139431146	28.100216498964986	27.768130218488015	22.188721888235534
76-77	21.952088845437267	28.01825478451917	27.710089277694262	22.319567092349303
78-79	21.96682260953976	28.11636366767878	27.789491234881446	22.127322487900013
80-81	21.900713807368536	28.01222965797313	27.822400124200737	22.264656410457597
82-83	21.840846112185982	27.96829638900531	27.97701576712347	22.21384173168524
84-85	21.849425171874326	27.975004664647713	27.792018907372057	22.383551256105903
86-87	22.066361787341588	27.928357544866238	27.808104240262608	22.197176427529563
88-89	21.785194009286037	27.999291945642472	27.829179494171708	22.38633455089978
90-91	21.830329794819797	27.9156138793547	27.909382670158056	22.344673655667446
92-93	21.967075632899395	28.00643881718369	27.78115915214187	22.24532639777505
94-95	21.82943430454835	27.817850719435217	27.917262976756046	22.43545199926039
96-97	21.945543469173867	27.795875257633444	27.89248817245274	22.366093100739953
98-99	21.84363239249522	27.94762447117548	27.80661656002171	22.402126576307584
100	21.990190032832245	27.75099829069479	27.758580850901986	22.500230825570977
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3395.0
1	1943.0
2	472.0
3	412.5
4	347.0
5	299.5
6	271.0
7	270.5
8	264.0
9	265.0
10	265.0
11	267.5
12	295.0
13	318.5
14	358.0
15	445.5
16	583.5
17	721.5
18	928.5
19	1307.0
20	1865.5
21	2739.0
22	4221.5
23	6798.0
24	10248.5
25	14329.0
26	20350.0
27	28536.5
28	39096.0
29	51079.0
30	62376.5
31	73906.0
32	85784.0
33	98201.5
34	110515.0
35	120542.5
36	129935.0
37	139836.5
38	147211.5
39	149723.0
40	151784.5
41	155284.0
42	156624.5
43	156652.5
44	154585.0
45	152460.5
46	151824.0
47	147815.0
48	142571.0
49	137267.0
50	133268.5
51	130010.0
52	120266.0
53	111858.0
54	108211.5
55	103482.5
56	95300.0
57	84622.5
58	75449.5
59	69263.0
60	62521.5
61	53330.5
62	45918.5
63	39334.5
64	32374.0
65	25878.5
66	20197.5
67	15156.0
68	11151.5
69	8363.5
70	5982.0
71	4340.5
72	3360.5
73	2429.5
74	1731.5
75	1164.0
76	775.0
77	559.0
78	367.0
79	240.5
80	183.5
81	138.5
82	107.0
83	91.0
84	76.0
85	56.5
86	46.5
87	47.0
88	39.0
89	28.5
90	21.5
91	16.5
92	13.5
93	10.0
94	8.5
95	3.5
96	2.0
97	1.5
98	1.5
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07777798758659493
2	0.08014431234721968
3	0.08145893721423342
4	0.08361014154207409
5	0.08155454629547079
6	0.08172186218763616
7	0.08107650088928396
8	0.08086138045649989
9	0.08059845548309715
10-11	0.0804191884557771
12-13	0.07931968402154742
14-15	0.07801701028968834
16-17	0.07661872747659192
18-19	0.07604507298916774
20-21	0.07562678325875427
22-23	0.07753896488350152
24-25	0.07776603645144027
26-27	0.07724018650463477
28-29	0.0731528982817375
30-31	0.07838749547948312
32-33	0.07666653201721059
34-35	0.07410898909411112
36-37	0.07102559622420616
38-39	0.07273460855132403
40-41	0.07498142196040207
42-43	0.0796065112652595
44-45	0.0808972338619639
46-47	0.08393282219125019
48-49	0.08733889571033125
50-51	0.08846230241487027
52-53	0.0885698626312623
54-55	0.0885340092257983
56-57	0.08637085376280294
58-59	0.08530720273403729
60-61	0.08323965635227931
62-63	0.08363404381238343
64-65	0.0829169757031032
66-67	0.08246283256722572
68-69	0.08194893375557491
70-71	0.08000089872536363
72-73	0.07978577829257956
74-75	0.0792001726700007
76-77	0.07934358629185675
78-79	0.07949895104886748
80-81	0.08063430888856116
82-83	0.08028772596907571
84-85	0.07942724423793945
86-87	0.08032357937453974
88-89	0.07727603991009878
90-91	0.07490971514947405
92-93	0.07461093677060729
94-95	0.07486191060885536
96-97	0.07415679363472981
98-99	0.07377435730978037
100	0.0730692403356548
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4183703.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.315577956005306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.1993314850149	47.57069698718912
2	11.224558508545122	13.315823504707017
3	3.325554657444473	5.917715895918126
4	1.526833524786213	3.62260051861196
5	0.8753676591808722	2.5961469314154453
6	0.5832890098511723	2.0758874840825015
7	0.3920497065857639	1.6278258453531818
8	0.2961556711528189	1.4053315839502483
9	0.21362850621305424	1.1404348482514846
>10	1.2476866506102695	13.370576356899882
>50	0.07864526762549093	3.2007424978617616
>100	0.03609195727924902	3.7937774185795714
>500	7.266561713685106E-4	0.25810931282287486
>1k	8.073953922747268E-5	0.1043308143568068
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.390227030934079E-5	0.0
4	0.0	0.0	0.0	2.390227030934079E-5	0.0
5	0.0	0.0	0.0	2.390227030934079E-5	0.0
6	0.0	0.0	0.0	2.390227030934079E-5	0.0
7	0.0	0.0	0.0	2.390227030934079E-5	0.0
8	0.0	0.0	0.0	4.780454061868158E-5	0.0
9	0.0	0.0	0.0	7.170681092802237E-5	0.0
10-11	0.0	0.0	0.0	7.170681092802237E-5	0.0
12-13	0.0	0.0	0.0	7.170681092802237E-5	0.0
14-15	0.0	1.1951135154670395E-5	0.0	9.560908123736316E-5	0.0
16-17	0.0	2.390227030934079E-5	0.0	9.560908123736316E-5	0.0
18-19	0.0	2.390227030934079E-5	0.0	9.560908123736316E-5	0.0
20-21	0.0	2.390227030934079E-5	0.0	9.560908123736316E-5	0.0
22-23	0.0	2.390227030934079E-5	0.0	1.0756021639203356E-4	0.0
24-25	0.0	2.390227030934079E-5	0.0	1.3146248670137435E-4	0.0
26-27	1.1951135154670395E-5	2.390227030934079E-5	0.0	1.9121816247472632E-4	0.0
28-29	2.390227030934079E-5	2.390227030934079E-5	0.0	6.573124335068717E-4	0.0
30-31	2.390227030934079E-5	2.390227030934079E-5	0.0	0.002832419031656884	0.0
32-33	2.390227030934079E-5	2.390227030934079E-5	0.0	0.008067016229402518	0.0
34-35	2.390227030934079E-5	2.390227030934079E-5	0.0	0.01539306207921547	0.0
36-37	2.390227030934079E-5	2.390227030934079E-5	0.0	0.030750270752966928	0.0
38-39	2.390227030934079E-5	2.390227030934079E-5	0.0	0.062205658480059406	0.0
40-41	2.390227030934079E-5	2.390227030934079E-5	0.0	0.10199098740995716	0.0
42-43	2.390227030934079E-5	2.390227030934079E-5	0.0	0.1439275206676956	0.0
44-45	2.390227030934079E-5	2.390227030934079E-5	0.0	0.1927240055042148	0.0
46-47	2.390227030934079E-5	2.390227030934079E-5	0.0	0.2527545573861242	0.0
48-49	2.390227030934079E-5	2.390227030934079E-5	0.0	0.3234455218259996	0.0
50-51	2.390227030934079E-5	2.390227030934079E-5	0.0	0.4011996071422852	0.0
52-53	2.390227030934079E-5	2.390227030934079E-5	0.0	0.4803639264068219	0.0
54-55	2.390227030934079E-5	2.390227030934079E-5	0.0	0.5684079390912786	0.0
56-57	2.390227030934079E-5	2.390227030934079E-5	0.0	0.6714745286651562	0.0
58-59	2.390227030934079E-5	2.390227030934079E-5	0.0	0.7932207424857836	0.0
60-61	2.390227030934079E-5	2.390227030934079E-5	0.0	0.9259380983783982	0.0
62-63	4.780454061868158E-5	2.390227030934079E-5	0.0	1.062682986818137	0.0
64-65	4.780454061868158E-5	2.390227030934079E-5	0.0	1.2043517429415997	1.1951135154670395E-5
66-67	4.780454061868158E-5	2.390227030934079E-5	0.0	1.371070078349252	2.390227030934079E-5
68-69	4.780454061868158E-5	2.390227030934079E-5	0.0	1.559360212711084	2.390227030934079E-5
70-71	4.780454061868158E-5	2.390227030934079E-5	0.0	1.7636887704504838	2.390227030934079E-5
72-73	4.780454061868158E-5	2.390227030934079E-5	0.0	1.9796457826953777	2.390227030934079E-5
74-75	4.780454061868158E-5	2.390227030934079E-5	0.0	2.197837657214195	2.390227030934079E-5
76-77	4.780454061868158E-5	2.390227030934079E-5	0.0	2.434171354897802	2.390227030934079E-5
78-79	4.780454061868158E-5	2.390227030934079E-5	0.0	2.7034184787973716	2.390227030934079E-5
80-81	4.780454061868158E-5	2.390227030934079E-5	0.0	3.0015753986360885	2.390227030934079E-5
82-83	4.780454061868158E-5	2.390227030934079E-5	0.0	3.3109783366553502	2.390227030934079E-5
84-85	4.780454061868158E-5	2.390227030934079E-5	0.0	3.619891278133271	2.390227030934079E-5
86-87	4.780454061868158E-5	2.390227030934079E-5	0.0	3.942225344389886	2.390227030934079E-5
88	4.780454061868158E-5	2.390227030934079E-5	0.0	4.210145892287287	2.390227030934079E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3310	0.0	35.07394	1
GTATCAA	5605	0.0	34.968422	1
TCAACGC	5445	0.0	34.443	4
ATCAACG	5505	0.0	33.98181	3
CAACGCA	5735	0.0	32.618984	5
AACGCAG	6045	0.0	31.100983	6
GTACATG	14580	0.0	29.464853	1
TATCAAC	6520	0.0	29.268066	2
TACATGG	14640	0.0	27.963575	2
ACATGGG	15205	0.0	26.151987	3
ACGCAGA	7530	0.0	24.967222	7
CGCAGAG	7870	0.0	23.8883	8
CATGGGG	10830	0.0	22.525003	4
GAGTACA	9965	0.0	21.036484	1
GCGGGTA	250	1.0741132E-8	20.67961	9
AGTACAT	10245	0.0	19.635746	2
GCAGAGT	9650	0.0	18.994457	9
GTATAGG	1940	0.0	18.897678	1
GTACTAG	945	0.0	17.408117	1
CATGGGA	5635	0.0	17.099598	4
>>END_MODULE