FastQCFastQC Report
Wed 25 May 2016
SRR1032923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032923_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4183703
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66160.15813742036659867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52900.12644300993641278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53050.050.393761
GTATCAA101350.043.6230241
TCAACGC116900.037.0607724
ATCAACG118350.036.607153
CAACGCA120850.035.8053675
TATCAAC123900.035.2327042
AACGCAG127100.034.1552856
ACGCAGA150350.028.8415687
CGCAGAG153250.028.295458
GTACATG144100.027.7469651
TACATGG143800.027.0857452
GTGGTAT27050.025.3591961
ACATGGG149950.025.0967833
GCAGAGT173200.024.8978379
TGGTATC28350.022.8978822
CATGGGG107600.021.77144
ACACCGT7500.020.6944756
GAGTACA102400.020.0966641
CAGAGTA168950.020.04650710-11
GAGTACT104250.019.87120212-13
Produced by FastQC (version 0.11.5)