FastQCFastQC Report
Wed 25 May 2016
SRR1032922_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032922_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4157576
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48550.11677477453208311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG146200.029.0948731
TACATGG146650.027.819732
ACATGGG148950.025.8439353
GAGTACA99550.022.6628881
CATGGGG105350.020.5675094
TATACTG21400.020.2061735
AGTACAT104200.020.1629962
CGCGCGT4300.019.674977
GTATCAA111100.018.9530541
TAGCGCG4500.018.8007554
GCGTTAG2753.2077878E-818.8007531
TCAACGC111350.018.319554
GTATAGA14450.018.2152631
CAACGCA112450.018.1819235
ATCAACG113650.017.9488053
AACGCAG115850.017.6077446
ACACCGT8550.017.5911376
CGTTAGG2253.8761533E-516.711782
TATCAAC123750.016.6358172
GTGTAAG16750.016.5558871
Produced by FastQC (version 0.11.5)