##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032922_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4157576
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.828775950217146	33.0	31.0	34.0	30.0	34.0
2	31.92310856133478	34.0	31.0	34.0	30.0	34.0
3	32.03237319053218	34.0	31.0	34.0	30.0	34.0
4	35.35615584657983	37.0	35.0	37.0	33.0	37.0
5	35.39669244771473	37.0	35.0	37.0	35.0	37.0
6	35.503019547928886	37.0	37.0	37.0	35.0	37.0
7	35.451374310415495	37.0	37.0	37.0	35.0	37.0
8	35.452757808877095	37.0	37.0	37.0	35.0	37.0
9	37.14107595387312	39.0	39.0	39.0	35.0	39.0
10-11	37.13016069940754	39.0	38.0	39.0	35.0	39.0
12-13	37.0757347550592	39.0	38.0	39.0	35.0	39.0
14-15	38.57919891302047	41.0	39.0	41.0	35.0	41.0
16-17	38.59554593830636	41.0	39.0	41.0	35.0	41.0
18-19	38.60238069009442	41.0	39.5	41.0	35.0	41.0
20-21	38.63223209870367	41.0	39.5	41.0	35.0	41.0
22-23	38.61385756989169	41.0	39.0	41.0	35.0	41.0
24-25	38.63464913209043	41.0	39.0	41.0	35.0	41.0
26-27	38.59994910495924	41.0	39.0	41.0	35.0	41.0
28-29	38.579246897711556	41.0	39.0	41.0	35.0	41.0
30-31	38.531057880842106	41.0	39.0	41.0	34.0	41.0
32-33	38.47984522712272	41.0	39.0	41.0	34.0	41.0
34-35	38.40565668553022	41.0	39.0	41.0	34.0	41.0
36-37	38.316173534771224	41.0	39.0	41.0	34.0	41.0
38-39	38.218632924569505	41.0	39.0	41.0	34.0	41.0
40-41	38.131070724864685	41.0	38.0	41.0	33.0	41.0
42-43	38.0112183156724	40.5	38.0	41.0	33.0	41.0
44-45	37.87037290478875	40.0	38.0	41.0	33.0	41.0
46-47	37.73550140755094	40.0	38.0	41.0	33.0	41.0
48-49	37.58872778272725	40.0	37.5	41.0	33.0	41.0
50-51	37.43586383027033	40.0	37.0	41.0	32.0	41.0
52-53	37.26862190853517	40.0	37.0	41.0	32.0	41.0
54-55	37.082639499554546	40.0	36.0	41.0	31.5	41.0
56-57	36.87881580517109	40.0	36.0	41.0	31.0	41.0
58-59	36.64775123774045	39.0	35.0	41.0	31.0	41.0
60-61	36.37833162881448	39.0	35.0	41.0	31.0	41.0
62-63	36.11194215090717	38.5	35.0	41.0	31.0	41.0
64-65	35.813181647190575	38.0	35.0	40.0	30.0	41.0
66-67	35.48550802198204	37.0	35.0	40.0	30.0	41.0
68-69	35.15023225071532	37.0	35.0	40.0	30.0	41.0
70-71	34.79367051859064	36.0	34.0	39.0	29.0	41.0
72-73	34.48986500787959	36.0	34.0	39.0	29.0	41.0
74-75	34.21047011527871	35.0	34.0	38.5	29.0	40.0
76-77	33.894384612572324	35.0	34.0	37.0	29.0	39.5
78-79	33.543657049203674	35.0	34.0	37.0	29.0	39.0
80-81	33.24416102074863	35.0	34.0	36.5	28.5	39.0
82-83	32.95345521043993	35.0	34.0	36.0	28.0	37.5
84-85	32.81840584513668	35.0	34.0	36.0	28.0	37.0
86-87	32.62418413998926	35.0	34.0	35.0	28.0	37.0
88-89	32.41660669582468	35.0	34.0	35.0	27.5	36.0
90-91	32.23881499700788	35.0	34.0	35.0	27.0	36.0
92-93	32.067959190643776	35.0	33.5	35.0	27.0	36.0
94-95	31.898948570032154	35.0	33.0	35.0	27.0	36.0
96-97	31.736254851384558	35.0	33.0	35.0	26.0	35.5
98-99	31.566948265046747	35.0	33.0	35.0	25.0	35.0
100	31.43915493066152	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3246048207516168
1101	2	0.4285029518065606
1101	3	0.41299806880081746
1101	4	0.33323870882496465
1101	5	0.3269059159107215
1101	6	0.3173269069156035
1101	7	0.35217725792406185
1101	8	0.3500828929639397
1101	9	0.43552839198146387
1101	10-11	0.4319059196144579
1101	12-13	0.24889267945119542
1101	14-15	0.3482497644166145
1101	16-17	0.328752634607838
1101	18-19	0.3565785707688178
1101	20-21	0.35945776524850714
1101	22-23	0.3443465655206168
1101	24-25	0.32884652945984527
1101	26-27	0.3783083570626573
1101	28-29	0.3893124558168566
1101	30-31	0.3814843228182099
1101	32-33	0.4042749937112333
1101	34-35	0.40465173056178116
1101	36-37	0.4199229526375561
1101	38-39	0.4466254378763992
1101	40-41	0.48793701327472405
1101	42-43	0.44176761190317393
1101	44-45	0.42816679041128225
1101	46-47	0.370023106012475
1101	48-49	0.3468912021522925
1101	50-51	0.35636870451741487
1101	52-53	0.3688173130718013
1101	54-55	0.3661911403898088
1101	56-57	0.3859177818977031
1101	58-59	0.33145866206324115
1101	60-61	0.4309632155491272
1101	62-63	0.4057085255033428
1101	64-65	0.37306720419095285
1101	66-67	0.4046247176411839
1101	68-69	0.3885964987315944
1101	70-71	0.3377936627884921
1101	72-73	0.29787595221755225
1101	74-75	0.36921629456583105
1101	76-77	0.35705311009733265
1101	78-79	0.3456657808303163
1101	80-81	0.3218544502227445
1101	82-83	0.31006717365074365
1101	84-85	0.3076322123488282
1101	86-87	0.25829197327656317
1101	88-89	0.27695581058053875
1101	90-91	0.2870553301833567
1101	92-93	0.3850567451706368
1101	94-95	0.3609818017125548
1101	96-97	0.3840536125003666
1101	98-99	0.3350167657688914
1101	100	0.3929288758578302
1102	1	0.26291901048509203
1102	2	0.22609489052615217
1102	3	0.30178797224800746
1102	4	0.2410567518333906
1102	5	0.21944349689927378
1102	6	0.1676365685920942
1102	7	0.20304207282784148
1102	8	0.176516152399536
1102	9	0.2377093582796732
1102	10-11	0.25582244352091976
1102	12-13	0.2584842838078458
1102	14-15	0.29821866406963693
1102	16-17	0.302187942346805
1102	18-19	0.24464145189200792
1102	20-21	0.251529981478015
1102	22-23	0.3054296510311758
1102	24-25	0.3725149339140401
1102	26-27	0.4011766210517038
1102	28-29	0.4265811038262086
1102	30-31	0.4176255812831826
1102	32-33	0.44445714223476784
1102	34-35	0.4232431502134233
1102	36-37	0.44372608320971096
1102	38-39	0.4349254876564572
1102	40-41	0.3986168985405314
1102	42-43	0.44905534295078553
1102	44-45	0.41377738613007864
1102	46-47	0.3973332407615615
1102	48-49	0.4251156927655444
1102	50-51	0.40843104086935966
1102	52-53	0.39166674790939027
1102	54-55	0.4477208313504235
1102	56-57	0.4375117647364064
1102	58-59	0.3683208552318362
1102	60-61	0.44132488029024586
1102	62-63	0.39155301483363303
1102	64-65	0.37526548009506655
1102	66-67	0.44302320911940996
1102	68-69	0.4892051380748157
1102	70-71	0.4868582818250573
1102	72-73	0.3484619855678375
1102	74-75	0.37731021846315116
1102	76-77	0.3474789191664698
1102	78-79	0.33277081656968477
1102	80-81	0.36664947343734156
1102	82-83	0.3948886312951103
1102	84-85	0.35291128790136383
1102	86-87	0.3869832302172753
1102	88-89	0.3276701086768057
1102	90-91	0.3914030583321946
1102	92-93	0.359937666124722
1102	94-95	0.3746746689625695
1102	96-97	0.3345547620853395
1102	98-99	0.30193191745965464
1102	100	0.29322535712829634
1103	1	0.08204818131115843
1103	2	0.1744439046666919
1103	3	0.19354562718729795
1103	4	0.12426372434885735
1103	5	0.11129918634105707
1103	6	0.04717855893758127
1103	7	0.08896101903234666
1103	8	0.12362842358559334
1103	9	0.17314609727506536
1103	10-11	0.17715399715098812
1103	12-13	0.14502160148959575
1103	14-15	0.17262609734725487
1103	16-17	0.29860874036651097
1103	18-19	0.288649988434841
1103	20-21	0.2738045516108656
1103	22-23	0.22038472012193466
1103	24-25	0.23216299005010654
1103	26-27	0.27512923079613927
1103	28-29	0.2754246266206479
1103	30-31	0.2858799355704491
1103	32-33	0.27077723808234566
1103	34-35	0.2567994177759729
1103	36-37	0.21127025293760227
1103	38-39	0.19633213194487098
1103	40-41	0.24165611894000705
1103	42-43	0.2577650486493255
1103	44-45	0.22953096509640147
1103	46-47	0.25877466844556096
1103	48-49	0.24102339328145206
1103	50-51	0.2585985007671212
1103	52-53	0.1909623134506404
1103	54-55	0.273506450631551
1103	56-57	0.2313461486413999
1103	58-59	0.228139529107807
1103	60-61	0.263442218163334
1103	62-63	0.29322912203436147
1103	64-65	0.2374732736494849
1103	66-67	0.22199311158450996
1103	68-69	0.2722359312776277
1103	70-71	0.22299747573635642
1103	72-73	0.21646701172137028
1103	74-75	0.21070293808163854
1103	76-77	0.2067315380545125
1103	78-79	0.1984696753161046
1103	80-81	0.19908824193546337
1103	82-83	0.2220420204138449
1103	84-85	0.2081902102005273
1103	86-87	0.21746225130506502
1103	88-89	0.18608424977672655
1103	90-91	0.14617493487281763
1103	92-93	0.129281334556957
1103	94-95	0.10467398083576995
1103	96-97	0.15737342370699992
1103	98-99	0.15841081977487192
1103	100	0.17312083726641347
1104	1	0.009509366312272505
1104	2	0.10735522428566568
1104	3	0.13885183460288886
1104	4	0.09159112852779572
1104	5	0.10747675235985099
1104	6	0.08453549243075287
1104	7	0.10761996455124034
1104	8	0.11376847128959611
1104	9	0.10519152452573621
1104	10-11	0.11535922333737858
1104	12-13	0.1509856058596668
1104	14-15	0.21226637396349446
1104	16-17	0.155144719619571
1104	18-19	0.15480833164887997
1104	20-21	0.14420681846065975
1104	22-23	0.15964310271324678
1104	24-25	0.17084854616126677
1104	26-27	0.19518019561208177
1104	28-29	0.24596311990504205
1104	30-31	0.22363369879295902
1104	32-33	0.22496449775179173
1104	34-35	0.21823113460317245
1104	36-37	0.27338484880379355
1104	38-39	0.27973606050095867
1104	40-41	0.2942188951177016
1104	42-43	0.25499616411789816
1104	44-45	0.18982028977983134
1104	46-47	0.19123488903107244
1104	48-49	0.196162199982723
1104	50-51	0.23076673269851966
1104	52-53	0.18926866862198466
1104	54-55	0.27631477066322674
1104	56-57	0.24945976626261768
1104	58-59	0.21347527311624503
1104	60-61	0.27055746546496806
1104	62-63	0.25937951700804973
1104	64-65	0.27307830433246494
1104	66-67	0.34117144404077493
1104	68-69	0.2967590186595075
1104	70-71	0.27032707805502554
1104	72-73	0.34182245164409863
1104	74-75	0.31543020498602914
1104	76-77	0.3270706338621068
1104	78-79	0.3140869777328277
1104	80-81	0.2681265121305074
1104	82-83	0.2630770967555307
1104	84-85	0.25245815324396403
1104	86-87	0.17211497798093944
1104	88-89	0.16384698024476307
1104	90-91	0.18366924482071312
1104	92-93	0.11840654045058585
1104	94-95	0.16478371313583295
1104	96-97	0.2696380680851149
1104	98-99	0.25213481057762266
1104	100	0.21449122453933
1105	1	-0.07029246618051843
1105	2	-0.07264266044318646
1105	3	-0.038294191470612304
1105	4	-0.04125747372329869
1105	5	-0.048693437903217784
1105	6	0.0010620502166176493
1105	7	0.018855923047389922
1105	8	-0.01710505219548253
1105	9	-0.0026473085125715556
1105	10-11	-0.02452392184815011
1105	12-13	0.043677998120010386
1105	14-15	0.09332023822988589
1105	16-17	0.10496142859165758
1105	18-19	0.024942484371464957
1105	20-21	-0.01115441975227327
1105	22-23	0.006955929049489384
1105	24-25	0.03231177105912053
1105	26-27	-0.0033803067091611183
1105	28-29	-0.03623069459681716
1105	30-31	-0.03470245954066087
1105	32-33	-0.033898808417006876
1105	34-35	0.007263787709831604
1105	36-37	0.04963980299147153
1105	38-39	0.015331077924116698
1105	40-41	-0.005063123407616388
1105	42-43	0.010790323186284922
1105	44-45	0.002436643609030398
1105	46-47	-0.0063481910263831764
1105	48-49	-0.0036310044304030953
1105	50-51	-0.02954304319406731
1105	52-53	0.016836190303514798
1105	54-55	-0.028515868482649864
1105	56-57	-0.04066929310970124
1105	58-59	-0.035263065059950804
1105	60-61	-0.054276666661543516
1105	62-63	-0.022895276196365444
1105	64-65	0.00791701490769725
1105	66-67	-0.04777758627439965
1105	68-69	0.013999452624084086
1105	70-71	0.01914423791386355
1105	72-73	-0.07124444102259275
1105	74-75	-0.06428196505950723
1105	76-77	-0.05760584098286614
1105	78-79	-0.015068750755418137
1105	80-81	0.013064445621928655
1105	82-83	-0.04749249566511793
1105	84-85	-0.09680677611711275
1105	86-87	-0.07796369062286601
1105	88-89	-0.0289544319770485
1105	90-91	0.03357980503357538
1105	92-93	0.009245760360855115
1105	94-95	-0.025038005688962528
1105	96-97	0.013608557269392918
1105	98-99	-0.023910035953893072
1105	100	-0.023731335384074015
1106	1	-0.04994847221919585
1106	2	-0.024778214532897636
1106	3	-0.032766384980391194
1106	4	-0.005951935546328002
1106	5	-0.054039760972614204
1106	6	-0.004773470551548087
1106	7	-0.0013448392679791255
1106	8	-0.00674859665594596
1106	9	0.05731234861551826
1106	10-11	0.05255659218769182
1106	12-13	0.07572894778407147
1106	14-15	0.10132760977192135
1106	16-17	0.04181433606656526
1106	18-19	0.07170039143834117
1106	20-21	0.07063467526173639
1106	22-23	0.0030259842000504023
1106	24-25	0.057151411118418594
1106	26-27	0.10388170774831451
1106	28-29	0.08274868854655892
1106	30-31	0.025251034801478056
1106	32-33	0.10025314123345908
1106	34-35	0.09759705417339859
1106	36-37	0.061367797170397864
1106	38-39	0.09725214156146933
1106	40-41	0.0981027634895355
1106	42-43	0.08896292539502326
1106	44-45	-0.0016399330441245752
1106	46-47	0.04382623706708699
1106	48-49	0.09988701130552613
1106	50-51	0.06935777080369832
1106	52-53	0.03999742748491997
1106	54-55	0.074167833541118
1106	56-57	0.05994222526474857
1106	58-59	0.028686039678511577
1106	60-61	0.05301297407778094
1106	62-63	0.026298206381227374
1106	64-65	0.07414380033054613
1106	66-67	0.04744149439093803
1106	68-69	0.05759606300680531
1106	70-71	0.042505021854239544
1106	72-73	0.004917817906218147
1106	74-75	-0.01856748140746589
1106	76-77	0.010040722817691972
1106	78-79	0.005303932037058701
1106	80-81	-0.01716338194926692
1106	82-83	0.005054605020355041
1106	84-85	0.01770161207210208
1106	86-87	-0.049146290995523145
1106	88-89	-0.0498866532321145
1106	90-91	-0.0017457090702848177
1106	92-93	-0.03043323074432891
1106	94-95	-0.013394165414776893
1106	96-97	-0.019068446443576192
1106	98-99	0.0011557145969334215
1106	100	0.0328659907318567
1107	1	0.09593777611408072
1107	2	0.16312596527782475
1107	3	0.17303969814426523
1107	4	0.15833592747360825
1107	5	0.14186427249189393
1107	6	0.13243404026181338
1107	7	0.1426947026683436
1107	8	0.15246298339543785
1107	9	0.18953565262540195
1107	10-11	0.17329237831082622
1107	12-13	0.17588571101214256
1107	14-15	0.23439311596724366
1107	16-17	0.25224856766759274
1107	18-19	0.26073087820213914
1107	20-21	0.25978635499116365
1107	22-23	0.25828829964120104
1107	24-25	0.21665329532603295
1107	26-27	0.2661491290465676
1107	28-29	0.2506233043877302
1107	30-31	0.21321288925202708
1107	32-33	0.21680113872931628
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2216	50-51	-0.2605831430549088
2216	52-53	-0.3016295616483333
2216	54-55	-0.3150968823460545
2216	56-57	-0.2939852808515937
2216	58-59	-0.27203879842988954
2216	60-61	-0.26925356944733636
2216	62-63	-0.2667178321821311
2216	64-65	-0.24158961453203887
2216	66-67	-0.18843827682075442
2216	68-69	-0.2339030674285496
2216	70-71	-0.23576837878091084
2216	72-73	-0.2630240946472142
2216	74-75	-0.24696232551331576
2216	76-77	-0.25968457335608974
2216	78-79	-0.17287058162762037
2216	80-81	-0.17148707994711998
2216	82-83	-0.2232538770824064
2216	84-85	-0.2539124194581177
2216	86-87	-0.18638810493445135
2216	88-89	-0.15612050370266672
2216	90-91	-0.1793570945876226
2216	92-93	-0.22502449242879408
2216	94-95	-0.17994981094680895
2216	96-97	-0.1364590540225592
2216	98-99	-0.10495623004419485
2216	100	-0.1772354243500196
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46168.0
3	7120.0
4	4713.0
5	4094.0
6	3546.0
7	4036.0
8	4753.0
9	5047.0
10	5369.0
11	5647.0
12	6160.0
13	6933.0
14	7528.0
15	8100.0
16	8127.0
17	8107.0
18	7753.0
19	7522.0
20	7335.0
21	7744.0
22	9198.0
23	12878.0
24	19719.0
25	26063.0
26	27066.0
27	24078.0
28	27291.0
29	35060.0
30	42878.0
31	52239.0
32	68319.0
33	92757.0
34	137707.0
35	223075.0
36	438539.0
37	907119.0
38	1338010.0
39	508945.0
40	833.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.24067360736854	18.04746966381492	12.283612550839122	24.42824417797742
2	16.165291460369673	18.98684938668744	39.50871929495594	25.339139857986954
3	19.639497078132077	23.735744806123105	28.426451901290285	28.19830621445454
4	12.657828077673091	15.204573207939854	37.03539214829611	35.10220656609095
5	13.612052765149063	37.88673175693908	34.56908726382791	13.932128214083948
6	33.34028189830623	36.01495786931001	16.92090310482905	13.723857127554712
7	29.98574276167737	31.26533170645639	20.7170695225113	18.031856009354943
8	26.683068648576825	35.8481514432011	18.89982996242998	18.5689499457921
9	25.937701970327737	16.633292292360963	19.645699664330582	37.783306072980714
10-11	25.4057678990046	26.108154545281675	27.399361689887392	21.08671586582634
12-13	25.66294687398527	23.305627252558285	27.66418988056883	23.367235992887615
14-15	22.70118549608371	25.29918422300801	24.612640421344974	27.38698985956331
16-17	22.860553570586124	28.69333088782532	25.295928132873886	23.15018740871467
18-19	22.95276420203624	27.825155975772713	26.83805056670706	22.384029255483988
20-21	23.756021811567436	26.983468503288353	26.59188903246522	22.668620652678985
22-23	23.507098806328937	26.64281563057636	26.637448004127535	23.212637558967167
24-25	23.421091872246752	26.821324558321898	26.484462903034995	23.27312066639636
26-27	23.12668791250054	26.851829564765296	26.757125939333836	23.26435658340033
28-29	23.10038212108492	27.01510143682919	26.76912723505694	23.11538920702895
30-31	23.220085163291422	27.19015176232844	26.470749294227364	23.119013780152773
32-33	23.07846077322387	27.01193975352182	26.776006594189788	23.13359287906452
34-35	23.24810953652385	26.83904542094689	26.79639461049725	23.11645043203201
36-37	23.440464196299505	26.982866057859912	26.48796787518226	23.08870187065833
38-39	23.140300431544965	27.31937516419564	26.561286208336128	22.979038195923266
40-41	23.031851800503343	27.000377411534544	26.82748538011696	23.140285407845155
42-43	23.059315336931267	26.928432660805125	26.73757583183032	23.274676170433295
44-45	23.033233342528444	27.00076690106976	26.74574702085653	23.220252735545262
46-47	23.184934759005323	27.123682810248727	26.708638126507267	22.982744304238683
48-49	23.125473474704325	27.00699713465807	26.70879984709189	23.15872954354571
50-51	22.947060189300387	27.010529754382762	26.832801106284403	23.209608950032443
52-53	23.090290817142055	26.984803884751603	26.78270176269737	23.142203535408974
54-55	23.066004249796766	27.230475284425122	26.631702655773925	23.071817810004188
56-57	22.983536917752716	27.014254460002697	26.728180707907363	23.274027914337232
58-59	23.08736659779282	26.622042896928626	26.92449485323756	23.366095652040993
60-61	23.161424741843444	27.031332877020148	26.779317530550493	23.027924850585915
62-63	22.84372916683968	27.034318424749195	26.825654333495937	23.296298074915185
64-65	22.893069710185603	27.055718465190644	26.7280350016874	23.32317682293635
66-67	22.853516008400383	26.872138004294072	26.682456600449484	23.591889386856067
68-69	23.03054636740973	26.996106376523056	26.745273305264007	23.22807395080321
70-71	23.055006710307637	26.883221871980258	26.760798323813173	23.300973093898932
72-73	22.916351992266563	26.89397682207159	26.859627917355137	23.330043268306706
74-75	22.873375011508738	27.188757122629276	26.68261341295875	23.25525445290324
76-77	22.947431638021662	26.927732267648103	26.637838124082684	23.486997970247547
78-79	23.063963784192726	27.084095070586113	26.66945325097625	23.182487894244915
80-81	22.97206640976567	26.887419545930825	26.824052827022214	23.31646121728129
82-83	22.912790018234816	26.92252375815563	26.945029932610254	23.2196562909993
84-85	22.867838455476374	26.930028696967057	26.771656196758848	23.430476650797726
86-87	23.085436728914924	27.050858081411494	26.646926214735245	23.21677897493834
88-89	22.780169010775715	27.09505147155104	26.640330742746226	23.48444877492702
90-91	22.916507708115756	26.80064034321087	26.740683384171987	23.542168564501388
92-93	23.072119380367024	27.008862111950144	26.718789752116486	23.20022875556635
94-95	22.881319930020485	26.775696452488297	26.878617412392323	23.464366205098898
96-97	22.97181911231377	26.775194945937443	26.838485056902734	23.414500884846056
98-99	22.889208500799636	27.005302319077117	26.694352637253573	23.41113654286967
100	22.98376692770261	26.706200742864517	26.609106540789192	23.700925788643683
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3516.0
1	2128.5
2	686.0
3	578.5
4	467.5
5	395.5
6	357.0
7	327.5
8	336.0
9	330.0
10	310.0
11	311.5
12	330.5
13	350.0
14	399.0
15	451.0
16	507.5
17	722.5
18	1012.0
19	1325.5
20	1838.5
21	2540.0
22	3723.0
23	5972.5
24	8936.5
25	12279.0
26	16965.0
27	23025.0
28	30407.0
29	38969.0
30	46511.5
31	53699.0
32	61897.0
33	71076.0
34	80788.5
35	89745.0
36	98305.0
37	107172.0
38	114283.0
39	119402.0
40	125808.5
41	133754.0
42	139214.0
43	142683.0
44	144670.0
45	146898.5
46	150284.0
47	151524.5
48	155685.0
49	154911.5
50	148611.0
51	145260.0
52	137780.5
53	132473.0
54	129673.5
55	122647.5
56	112514.5
57	102201.0
58	94270.5
59	87738.5
60	80741.0
61	71766.5
62	63789.5
63	55910.0
64	46986.0
65	38994.0
66	31600.0
67	24669.0
68	19531.5
69	15567.0
70	11796.5
71	8862.5
72	7092.5
73	5802.0
74	4610.0
75	3351.5
76	2170.0
77	1523.0
78	1130.0
79	771.5
80	554.5
81	346.0
82	210.0
83	139.0
84	93.5
85	73.5
86	61.5
87	49.0
88	34.5
89	27.5
90	25.0
91	20.5
92	13.5
93	10.5
94	8.0
95	5.5
96	4.5
97	3.5
98	2.5
99	1.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07333600155475209
2	0.07622229876254817
3	0.07728060773874007
4	0.07920480587727079
5	0.0780021820406891
6	0.07795407708722583
7	0.07684766315757066
8	0.07696792554122883
9	0.07675145325064413
10-11	0.07622229876254817
12-13	0.07535640960020935
14-15	0.07385312980448223
16-17	0.07263847972953472
18-19	0.07226566634019438
20-21	0.07195298414268314
22-23	0.07310750302580157
24-25	0.07339613274658119
26-27	0.07302331935724087
28-29	0.06907871317325287
30-31	0.07423796943218837
32-33	0.07238592872385255
34-35	0.06992054985886006
36-37	0.06691399026740581
38-39	0.06860968987698601
40-41	0.07119533112563667
42-43	0.07583745913484204
44-45	0.07682361068083902
46-47	0.07996245889431726
48-49	0.08290888729394243
50-51	0.08395517003176851
52-53	0.08389503883993943
54-55	0.08406340617706086
56-57	0.08183855207938472
58-59	0.08087645301011935
60-61	0.07854336276715086
62-63	0.07916872716217335
64-65	0.07845917909859014
66-67	0.07796610332559165
68-69	0.07743694883749569
70-71	0.0756811180360864
72-73	0.07575327546628131
74-75	0.07512791107125882
76-77	0.07524817345491701
78-79	0.07569314427445223
80-81	0.0766552433437176
82-83	0.07601785271032929
84-85	0.07528425217001446
86-87	0.07606595766379255
88-89	0.07331194907802047
90-91	0.07044970434695602
92-93	0.07030538948656621
94-95	0.0705098355387851
96-97	0.06971610380664117
98-99	0.0693793691323983
100	0.06850145373169365
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4157576.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.19026981039356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.07121929308605	33.28560693866678
2	15.655971513526298	15.08930982761336
3	6.062313169374523	8.76433521921781
4	2.95046246288032	5.687343286065637
5	1.6755467272403128	4.037252443281628
6	1.015802662261401	2.9371082621095796
7	0.7127938563947368	2.40448097811973
8	0.4849574262243265	1.8696183373043458
9	0.37797891738340844	1.6393415410212215
>10	1.8271732288150495	15.767316303698605
>50	0.11582929632202017	3.832464119488509
>100	0.04870419362460832	3.97184213212858
>500	8.481319835122775E-4	0.2954784044432282
>1k	3.9912088347400043E-4	0.4185022068410356
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	4855	0.11677477453208311	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.405247673163401E-5	0.0
10-11	0.0	0.0	0.0	2.405247673163401E-5	0.0
12-13	0.0	0.0	0.0	2.405247673163401E-5	0.0
14-15	0.0	0.0	0.0	2.405247673163401E-5	0.0
16-17	0.0	0.0	0.0	4.810495346326802E-5	0.0
18-19	0.0	0.0	0.0	4.810495346326802E-5	0.0
20-21	0.0	0.0	0.0	7.215743019490203E-5	0.0
22-23	0.0	0.0	0.0	1.6836733712143807E-4	0.0
24-25	0.0	0.0	0.0	2.525510056821571E-4	0.0
26-27	0.0	0.0	0.0	3.728133893403272E-4	0.0
28-29	0.0	0.0	0.0	0.0014431486038980407	0.0
30-31	0.0	0.0	0.0	0.005892856799250333	1.2026238365817005E-5
32-33	0.0	0.0	0.0	0.0147802469515891	2.405247673163401E-5
34-35	0.0	0.0	0.0	0.027804663101768913	2.405247673163401E-5
36-37	0.0	0.0	0.0	0.05073869966538194	2.405247673163401E-5
38-39	0.0	0.0	0.0	0.0927222978004491	2.405247673163401E-5
40-41	0.0	0.0	0.0	0.14418257176778007	2.405247673163401E-5
42-43	0.0	0.0	0.0	0.19698978443208254	2.405247673163401E-5
44-45	0.0	0.0	0.0	0.25750581588887367	2.405247673163401E-5
46-47	0.0	0.0	0.0	0.33466616124395565	2.405247673163401E-5
48-49	0.0	0.0	0.0	0.4209183428035952	2.405247673163401E-5
50-51	0.0	0.0	0.0	0.5182106111830548	2.405247673163401E-5
52-53	0.0	0.0	0.0	0.6194835644615997	2.405247673163401E-5
54-55	0.0	0.0	0.0	0.7222116925824087	2.405247673163401E-5
56-57	0.0	0.0	0.0	0.8510127054803087	2.405247673163401E-5
58-59	0.0	0.0	0.0	0.99616940255572	2.405247673163401E-5
60-61	0.0	0.0	0.0	1.1548435915543096	2.405247673163401E-5
62-63	0.0	0.0	0.0	1.312074631949001	2.405247673163401E-5
64-65	0.0	0.0	0.0	1.478277246164592	2.405247673163401E-5
66-67	0.0	0.0	0.0	1.6699875119540808	2.405247673163401E-5
68-69	0.0	0.0	0.0	1.893098286116718	2.405247673163401E-5
70-71	0.0	0.0	0.0	2.1312418582366264	2.405247673163401E-5
72-73	0.0	0.0	0.0	2.374797237621152	2.405247673163401E-5
74-75	0.0	0.0	0.0	2.620986363207792	2.405247673163401E-5
76-77	0.0	0.0	0.0	2.8872472806269807	2.405247673163401E-5
78-79	0.0	0.0	0.0	3.189165994800817	2.405247673163401E-5
80-81	0.0	1.2026238365817005E-5	0.0	3.52306247678936	2.405247673163401E-5
82-83	0.0	2.405247673163401E-5	0.0	3.865172879581756	2.405247673163401E-5
84-85	0.0	2.405247673163401E-5	0.0	4.199923224494272	2.405247673163401E-5
86-87	0.0	2.405247673163401E-5	0.0	4.553025609153026	2.405247673163401E-5
88	0.0	2.405247673163401E-5	0.0	4.842533245333338	2.405247673163401E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	14620	0.0	29.094873	1
TACATGG	14665	0.0	27.81973	2
ACATGGG	14895	0.0	25.843935	3
GAGTACA	9955	0.0	22.662888	1
CATGGGG	10535	0.0	20.567509	4
TATACTG	2140	0.0	20.206173	5
AGTACAT	10420	0.0	20.162996	2
CGCGCGT	430	0.0	19.67497	7
GTATCAA	11110	0.0	18.953054	1
TAGCGCG	450	0.0	18.800755	4
GCGTTAG	275	3.2077878E-8	18.800753	1
TCAACGC	11135	0.0	18.31955	4
GTATAGA	1445	0.0	18.215263	1
CAACGCA	11245	0.0	18.181923	5
ATCAACG	11365	0.0	17.948805	3
AACGCAG	11585	0.0	17.607744	6
ACACCGT	855	0.0	17.591137	6
CGTTAGG	225	3.8761533E-5	16.71178	2
TATCAAC	12375	0.0	16.635817	2
GTGTAAG	1675	0.0	16.555887	1
>>END_MODULE