FastQCFastQC Report
Wed 25 May 2016
SRR1032922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032922_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4157576
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76300.1835203974623675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61130.1470327902604787No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50500.12146500749475174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG149150.028.5041281
GTATCAA153950.027.8900261
TACATGG149400.027.6470242
ACATGGG149750.026.6399543
TCAACGC169250.025.043824
CAACGCA172350.024.6185865
ATCAACG172800.024.5565453
GGTATCA110100.024.2350231
AACGCAG176450.024.0465476
TATCAAC183000.023.264942
GAGTACA102550.022.1713491
TATACCG2005.922102E-721.1680265
ACGCAGA201250.020.9895467
CATGGGG102600.020.8626024
AGTACAT107200.020.2306862
CGCAGAG209000.020.1437058
AGAGTAC156500.019.61425810-11
GAGTACT104850.019.39814212-13
GCAGAGT222600.018.6116149
CTACACT20000.018.347174
Produced by FastQC (version 0.11.5)