##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032922_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4157576
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85156591244514	34.0	31.0	34.0	31.0	34.0
2	32.89880233097362	34.0	31.0	34.0	31.0	34.0
3	32.97498590524864	34.0	31.0	34.0	31.0	34.0
4	36.376614402238225	37.0	37.0	37.0	35.0	37.0
5	36.356264323249896	37.0	37.0	37.0	35.0	37.0
6	36.417543299268615	37.0	37.0	37.0	35.0	37.0
7	36.38297844705665	37.0	37.0	37.0	35.0	37.0
8	36.32114915037031	37.0	37.0	37.0	35.0	37.0
9	38.171825361701146	39.0	39.0	39.0	37.0	39.0
10-11	38.178835335782196	39.0	39.0	39.0	37.0	39.0
12-13	38.17164821521001	39.0	39.0	39.0	37.0	39.0
14-15	39.73928510266559	41.0	40.0	41.0	37.0	41.0
16-17	39.74847110431655	41.0	40.0	41.0	37.0	41.0
18-19	39.76014810072023	41.0	40.0	41.0	37.0	41.0
20-21	39.76347708376227	41.0	40.0	41.0	37.0	41.0
22-23	39.72785043496499	41.0	40.0	41.0	37.0	41.0
24-25	39.70582161336317	41.0	40.0	41.0	37.0	41.0
26-27	39.62944405586332	41.0	40.0	41.0	37.0	41.0
28-29	39.571400137965	41.0	40.0	41.0	37.0	41.0
30-31	39.470852847909455	41.0	39.5	41.0	36.0	41.0
32-33	39.35905392950123	41.0	39.0	41.0	35.5	41.0
34-35	39.27074754616632	41.0	39.0	41.0	35.0	41.0
36-37	39.18648053577373	41.0	39.0	41.0	35.0	41.0
38-39	39.094090282414555	41.0	39.0	41.0	35.0	41.0
40-41	38.99310824384209	41.0	39.0	41.0	35.0	41.0
42-43	38.85762533264575	41.0	39.0	41.0	35.0	41.0
44-45	38.69064486133266	41.0	39.0	41.0	35.0	41.0
46-47	38.476584793639375	41.0	38.0	41.0	35.0	41.0
48-49	38.271037017723785	40.5	38.0	41.0	34.5	41.0
50-51	38.10317213684127	40.0	38.0	41.0	34.0	41.0
52-53	37.94841008318309	40.0	37.5	41.0	34.0	41.0
54-55	37.78107952807116	40.0	37.0	41.0	34.0	41.0
56-57	37.549920554669356	40.0	36.5	41.0	33.0	41.0
58-59	37.35510920305486	40.0	36.0	41.0	33.0	41.0
60-61	37.136276041616554	39.0	35.5	41.0	33.0	41.0
62-63	36.87899511638513	39.0	35.0	41.0	33.0	41.0
64-65	36.57410604159732	38.5	35.0	41.0	32.0	41.0
66-67	36.27508805611731	37.5	35.0	40.5	32.0	41.0
68-69	35.964378161698065	37.0	35.0	40.0	32.0	41.0
70-71	35.627330083683376	36.5	35.0	39.0	31.5	41.0
72-73	35.28192028239532	36.0	35.0	39.0	31.0	41.0
74-75	34.93927555383233	35.5	35.0	39.0	31.0	40.5
76-77	34.60812935229566	35.0	35.0	37.0	31.0	39.5
78-79	34.28719787683977	35.0	35.0	37.0	31.0	39.0
80-81	33.98209281081091	35.0	34.0	36.5	30.5	39.0
82-83	33.70744082609674	35.0	34.0	36.0	30.0	38.0
84-85	33.46453919302978	35.0	34.0	36.0	30.0	37.0
86-87	33.35384199831825	35.0	34.0	35.5	30.0	37.0
88-89	33.19230544432621	35.0	34.0	35.0	30.0	36.5
90-91	33.04218756313775	35.0	34.0	35.0	30.0	36.0
92-93	32.90024487826561	35.0	34.0	35.0	30.0	36.0
94-95	32.81434747073776	35.0	34.0	35.0	30.0	36.0
96-97	32.766777444357004	35.0	34.0	35.0	30.0	36.0
98-99	32.66776999386181	35.0	34.0	35.0	30.0	35.0
100	32.587834353479046	35.0	34.0	35.0	30.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19956520316615212
1101	2	0.24994946427234055
1101	3	0.22940009437402864
1101	4	0.14075608058156064
1101	5	0.135506927643263
1101	6	0.1544619061639665
1101	7	0.11434767362913334
1101	8	0.049122601242345354
1101	9	0.10135621026013553
1101	10-11	0.21016447263918536
1101	12-13	0.2275614775622259
1101	14-15	0.2920842775345207
1101	16-17	0.28057214337169967
1101	18-19	0.2872269985274798
1101	20-21	0.3159808018429615
1101	22-23	0.28460088539628003
1101	24-25	0.2938020629606868
1101	26-27	0.29830421404582097
1101	28-29	0.28810488097676057
1101	30-31	0.276147471791127
1101	32-33	0.3226827373886181
1101	34-35	0.26630270215716934
1101	36-37	0.2667812195165311
1101	38-39	0.3177469652445666
1101	40-41	0.34081242654534805
1101	42-43	0.32104797383485817
1101	44-45	0.2822196998301365
1101	46-47	0.3191534999772827
1101	48-49	0.3913129733415417
1101	50-51	0.35647653086466846
1101	52-53	0.38797972198448605
1101	54-55	0.38954659003319136
1101	56-57	0.42556486870198285
1101	58-59	0.3993347450832374
1101	60-61	0.41028046265400064
1101	62-63	0.38940213931155654
1101	64-65	0.36896592476689705
1101	66-67	0.3509038756373499
1101	68-69	0.38863720544051716
1101	70-71	0.44841045551811476
1101	72-73	0.4515648394170597
1101	74-75	0.4161141234054355
1101	76-77	0.39493658569500667
1101	78-79	0.39433919221283986
1101	80-81	0.4047435452559611
1101	82-83	0.41692968929091734
1101	84-85	0.43847561745738517
1101	86-87	0.4426626986758748
1101	88-89	0.41172101221229696
1101	90-91	0.4137582238753339
1101	92-93	0.4241381546480838
1101	94-95	0.443300697382071
1101	96-97	0.445359754276744
1101	98-99	0.42789988579995253
1101	100	0.43701176582177936
1102	1	0.1543184218892577
1102	2	0.19258891616124885
1102	3	0.17098888905004372
1102	4	0.09896731937330117
1102	5	0.11671071540786215
1102	6	0.12043396433242037
1102	7	0.10854505454292962
1102	8	0.03457372332920983
1102	9	0.0943269749901603
1102	10-11	0.1535635242728688
1102	12-13	0.15983001530227625
1102	14-15	0.24050366543828972
1102	16-17	0.2156685616832732
1102	18-19	0.22612335038803622
1102	20-21	0.25878619251104595
1102	22-23	0.21843902245985447
1102	24-25	0.23101027206802627
1102	26-27	0.23376242891831112
1102	28-29	0.25889446484747936
1102	30-31	0.24837957001077626
1102	32-33	0.2660870743981576
1102	34-35	0.26698064599118254
1102	36-37	0.2914637831465612
1102	38-39	0.26419135291317275
1102	40-41	0.2018252731861736
1102	42-43	0.20099818056069552
1102	44-45	0.2050548169620896
1102	46-47	0.14068743622727453
1102	48-49	0.14812277899733317
1102	50-51	0.14051377715994562
1102	52-53	0.1587923444309851
1102	54-55	0.1428200073391963
1102	56-57	0.12781131703166437
1102	58-59	0.10522747353550699
1102	60-61	0.13048631923426512
1102	62-63	0.18565944197851536
1102	64-65	0.21854486750233804
1102	66-67	0.23016962619107773
1102	68-69	0.1949062841136211
1102	70-71	0.18560597972341952
1102	72-73	0.20474499186176587
1102	74-75	0.22897689721032322
1102	76-77	0.21636829082112286
1102	78-79	0.24992070169038527
1102	80-81	0.1800503259307007
1102	82-83	0.15040723473342865
1102	84-85	0.19233221935900957
1102	86-87	0.20482437209382454
1102	88-89	0.15141868997231
1102	90-91	0.12389495182939214
1102	92-93	0.1430282043271589
1102	94-95	0.10556351338816228
1102	96-97	0.15523702690578034
1102	98-99	0.23485024312754632
1102	100	0.23549171060470542
1103	1	0.1419519349839291
1103	2	0.1815326980872669
1103	3	0.16409253885571928
1103	4	0.10457696672245476
1103	5	0.11657497532749517
1103	6	0.13622128979094583
1103	7	0.12339633903057035
1103	8	0.08404721206608201
1103	9	0.14256937660923796
1103	10-11	0.12386896773288214
1103	12-13	0.16424628111151662
1103	14-15	0.20237861300397242
1103	16-17	0.2225682111156715
1103	18-19	0.2508723490692759
1103	20-21	0.23312707544371136
1103	22-23	0.25389970449719357
1103	24-25	0.17455748086549505
1103	26-27	0.24220565044517883
1103	28-29	0.24678578983091626
1103	30-31	0.28524996934113034
1103	32-33	0.23386577282045806
1103	34-35	0.23594000032086626
1103	36-37	0.1809996369092417
1103	38-39	0.20382283744775975
1103	40-41	0.18811034055190134
1103	42-43	0.20721017451649004
1103	44-45	0.17158923131434278
1103	46-47	0.16059126815089542
1103	48-49	0.1147293052672751
1103	50-51	0.1492723115818393
1103	52-53	0.16300641407884342
1103	54-55	0.1586860431915369
1103	56-57	0.17555317710437635
1103	58-59	0.1839911264895946
1103	60-61	0.17712340587507924
1103	62-63	0.1868627212326217
1103	64-65	0.22647670712920132
1103	66-67	0.19077295314274778
1103	68-69	0.1794036357680895
1103	70-71	0.20216579820876746
1103	72-73	0.16254208117968005
1103	74-75	0.1323808625174152
1103	76-77	0.16676566048001007
1103	78-79	0.1737780577187067
1103	80-81	0.1981716266600202
1103	82-83	0.23363664206772938
1103	84-85	0.18855553441456152
1103	86-87	0.21475460734835394
1103	88-89	0.18721118109500168
1103	90-91	0.18908412542681674
1103	92-93	0.19495821146293935
1103	94-95	0.17309514601140563
1103	96-97	0.21267770424877597
1103	98-99	0.20767489050968635
1103	100	0.20397173277558522
1104	1	0.079978544120479
1104	2	0.12160019990145798
1104	3	0.11873559953966151
1104	4	0.04277951438096039
1104	5	0.07037575544874386
1104	6	0.063189945919639
1104	7	0.10367695849836878
1104	8	0.04320452390131635
1104	9	0.06719021570176409
1104	10-11	0.09024092354590607
1104	12-13	0.14874178256760473
1104	14-15	0.1976307185234134
1104	16-17	0.18597114978101814
1104	18-19	0.17699095108152818
1104	20-21	0.14154382526847797
1104	22-23	0.18590945200954678
1104	24-25	0.15719412763972684
1104	26-27	0.1991363631883445
1104	28-29	0.21243075823811353
1104	30-31	0.22044694668063158
1104	32-33	0.23306094797653287
1104	34-35	0.2117696197442669
1104	36-37	0.2104118092171987
1104	38-39	0.18993657734959868
1104	40-41	0.22429243386511644
1104	42-43	0.23613735859031237
1104	44-45	0.13278858584735076
1104	46-47	0.16468688286212796
1104	48-49	0.18855471619931308
1104	50-51	0.17341816324817216
1104	52-53	0.18053196381394088
1104	54-55	0.14090058890811719
1104	56-57	0.21366324276470294
1104	58-59	0.191762671352393
1104	60-61	0.15167252883915694
1104	62-63	0.13106818470164683
1104	64-65	0.18110806381085354
1104	66-67	0.16613479096224637
1104	68-69	0.1847784076747061
1104	70-71	0.1706057231226481
1104	72-73	0.18763886847472122
1104	74-75	0.22590389094256835
1104	76-77	0.24270325078024513
1104	78-79	0.2559904926388512
1104	80-81	0.23639766966640963
1104	82-83	0.2352053568620036
1104	84-85	0.2381534422513667
1104	86-87	0.24226077414930813
1104	88-89	0.20778897158608345
1104	90-91	0.19385713110631997
1104	92-93	0.20636785227089405
1104	94-95	0.25614086513427736
1104	96-97	0.20218934601896876
1104	98-99	0.22189400136944926
1104	100	0.23940382372325075
1105	1	0.042432286480448056
1105	2	0.06836647913952021
1105	3	0.03213737302751696
1105	4	0.034041551943722936
1105	5	0.04140155062369644
1105	6	0.0358469491370812
1105	7	0.04202785414870647
1105	8	-0.01696531010556157
1105	9	0.04035633128463445
1105	10-11	0.023186758688353848
1105	12-13	0.03815347278058567
1105	14-15	0.08482515925685874
1105	16-17	0.08575781355310141
1105	18-19	0.10337852720345353
1105	20-21	0.10590995365430445
1105	22-23	0.10322129029150773
1105	24-25	0.11334145349167812
1105	26-27	0.09540043252470554
1105	28-29	0.07951171189140638
1105	30-31	0.15134397472468208
1105	32-33	0.14756146566131179
1105	34-35	0.14281041664483496
1105	36-37	0.1166390422198802
1105	38-39	0.11491414434246394
1105	40-41	0.16634467827913113
1105	42-43	0.17791147268135887
1105	44-45	0.13184113920227958
1105	46-47	0.17267095988025716
1105	48-49	0.1753018884157811
1105	50-51	0.23263974346704686
1105	52-53	0.18108310327711052
1105	54-55	0.20165331927783825
1105	56-57	0.2182232524504215
1105	58-59	0.1869303344230957
1105	60-61	0.23387527757834903
1105	62-63	0.2005023079217665
1105	64-65	0.19313049960124573
1105	66-67	0.23019946537748837
1105	68-69	0.2606609210621471
1105	70-71	0.23952736624300286
1105	72-73	0.2393420096523755
1105	74-75	0.19293236638872457
1105	76-77	0.1792379217442459
1105	78-79	0.2346122543257252
1105	80-81	0.2319353660247998
1105	82-83	0.21417066919848793
1105	84-85	0.21820432008888702
1105	86-87	0.17360315026678563
1105	88-89	0.2017843152063179
1105	90-91	0.16718525975933574
1105	92-93	0.18380023317556038
1105	94-95	0.14694472383416723
1105	96-97	0.14831435019384287
1105	98-99	0.20711997355920175
1105	100	0.24535154869347053
1106	1	0.04839063794796061
1106	2	0.11696238398950243
1106	3	0.036292365819186045
1106	4	0.05628920923502534
1106	5	0.0440196721045325
1106	6	0.04626163237838199
1106	7	0.03937403101131309
1106	8	0.03698584322795284
1106	9	0.026164072763840807
1106	10-11	0.034075338918476916
1106	12-13	0.038978648568736673
1106	14-15	0.085051482258244
1106	16-17	0.020430157349849765
1106	18-19	0.07718626188233912
1106	20-21	0.06965385887151143
1106	22-23	0.08869369121090642
1106	24-25	0.1060045505593834
1106	26-27	0.1084672388245167
1106	28-29	0.0820229409124309
1106	30-31	0.09550148363309319
1106	32-33	0.09178676521521112
1106	34-35	0.047971091100130536
1106	36-37	0.08945733098847342
1106	38-39	0.12495034752228662
1106	40-41	0.09583050642155655
1106	42-43	0.04320210966691462
1106	44-45	0.08611879268539724
1106	46-47	0.09840152627854337
1106	48-49	0.06957058248318759
1106	50-51	0.07704491136682634
1106	52-53	0.028774098639296142
1106	54-55	0.00648362590406748
1106	56-57	0.05971937287041129
1106	58-59	0.031808549272263065
1106	60-61	-0.005564682435803547
1106	62-63	0.024087672282668393
1106	64-65	0.009942112160061356
1106	66-67	0.019810665837191266
1106	68-69	0.049048914765045026
1106	70-71	0.09549987086721501
1106	72-73	0.039455022396168715
1106	74-75	-0.001758754779295657
1106	76-77	0.048992753368438
1106	78-79	0.040123230034993185
1106	80-81	0.07671242618039287
1106	82-83	0.06237211324549463
1106	84-85	0.09123414117258477
1106	86-87	0.10151215524461321
1106	88-89	0.04534348135162247
1106	90-91	0.08942768930806011
1106	92-93	0.05456724926511214
1106	94-95	0.1041881649298304
1106	96-97	0.07342821297707758
1106	98-99	0.04128301622437647
1106	100	0.07347144845682152
1107	1	0.06307841234712441
1107	2	0.06949315734798489
1107	3	0.07864455613431431
1107	4	0.05396281244163248
1107	5	0.04715635947648167
1107	6	0.058705126475750546
1107	7	0.03940004811755671
1107	8	0.056633026876198755
1107	9	0.06393115462016397
1107	10-11	0.027500836495633507
1107	12-13	0.05321422330287362
1107	14-15	0.1033492825932143
1107	16-17	0.09903667707406782
1107	18-19	0.07340565113077702
1107	20-21	0.11159094422387739
1107	22-23	0.11565808436190395
1107	24-25	0.10396195364565841
1107	26-27	0.10032591715552286
1107	28-29	0.11157090385432156
1107	30-31	0.13729410290526545
1107	32-33	0.13358624446657075
1107	34-35	0.12359987171088704
1107	36-37	0.17278239847034627
1107	38-39	0.1785512268191809
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2216	30-31	-0.34555587130515875
2216	32-33	-0.4027829555069147
2216	34-35	-0.4147729517380583
2216	36-37	-0.3614959866334644
2216	38-39	-0.42355236156953424
2216	40-41	-0.30767011049707094
2216	42-43	-0.3461785261555761
2216	44-45	-0.29368238976398686
2216	46-47	-0.2739416036653921
2216	48-49	-0.29051256942939574
2216	50-51	-0.2637162285059276
2216	52-53	-0.2577086874154446
2216	54-55	-0.2269137743849683
2216	56-57	-0.25410239511005983
2216	58-59	-0.29568462928538963
2216	60-61	-0.3787728772816905
2216	62-63	-0.30008391300005854
2216	64-65	-0.3347169593840036
2216	66-67	-0.32721320541308074
2216	68-69	-0.31485765475784433
2216	70-71	-0.3618607065131414
2216	72-73	-0.31663644635895594
2216	74-75	-0.34347161053130293
2216	76-77	-0.33036175753530017
2216	78-79	-0.3625413432475426
2216	80-81	-0.3963106352816155
2216	82-83	-0.37088174117491945
2216	84-85	-0.3585829711858608
2216	86-87	-0.3176292314088087
2216	88-89	-0.31958593298519844
2216	90-91	-0.3626686038300022
2216	92-93	-0.38497179395618986
2216	94-95	-0.41809397494557743
2216	96-97	-0.3917880041228017
2216	98-99	-0.39166799323680124
2216	100	-0.4059340209825919
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13.0
3	3.0
4	14.0
5	31.0
6	84.0
7	222.0
8	472.0
9	876.0
10	1348.0
11	2186.0
12	3419.0
13	5183.0
14	6349.0
15	8485.0
16	10634.0
17	15652.0
18	19102.0
19	11130.0
20	7911.0
21	7756.0
22	8184.0
23	8738.0
24	9718.0
25	11725.0
26	14695.0
27	18940.0
28	24273.0
29	31675.0
30	39710.0
31	50873.0
32	65151.0
33	85480.0
34	122057.0
35	195602.0
36	394135.0
37	900538.0
38	1459739.0
39	613544.0
40	1929.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.99623456937111	18.170253266399826	12.501879107428183	24.33163305680088
2	16.455777798569883	19.10879960630764	39.14845747302324	25.286965122099232
3	19.677224762168652	23.788161733013148	28.674696360949476	27.859917143868724
4	12.791927015990812	15.325391050197554	36.77150400221309	35.11117793159854
5	13.399344903277264	37.565717727298306	34.73123860808543	14.303698761338998
6	33.216257997415234	36.16502186322968	16.570184948082193	14.048535191272894
7	29.66646855267022	31.4383581248426	20.622632764238652	18.272540558248522
8	26.8837620445182	35.224246920300544	18.796638072539885	19.09535296264137
9	26.143168935726617	15.864450025760538	19.753641712539345	38.238739325973505
10-11	25.549827591846785	25.635995589737863	27.32478011225772	21.489396706157628
12-13	26.12094638466056	22.810238048937002	27.86321431307402	23.205601253328417
14-15	22.85544371799135	24.73891283785531	25.288729527542248	27.116913916611097
16-17	22.499540597694427	28.3229699228589	25.882341056423265	23.295148423023416
18-19	22.492099353204072	27.011661376318045	28.199277895675145	22.29696137480274
20-21	23.29111963317087	26.162432147963138	28.143129554336472	22.40331866452952
22-23	22.980148594207016	25.860500781166472	28.237781999533624	22.92156862509289
24-25	22.880669007461584	26.033261801026658	28.119255948558315	22.966813242953442
26-27	22.60889854256719	26.12230023741314	28.32458969862724	22.94421152139244
28-29	22.62434818502092	26.267632500232125	28.36794525596081	22.740074058786146
30-31	22.77544761918279	26.376511905138493	28.051243148397553	22.796797327281162
32-33	22.64901759935028	26.257091293472136	28.248210547485623	22.84568055969196
34-35	22.834329022695897	26.058736189253434	28.30883816806951	22.79809661998116
36-37	22.98876917896299	26.251880255104737	27.98726714041335	22.772083425518918
38-39	22.748940668407283	26.59398438978542	27.96865203837187	22.688422903435423
40-41	22.68985462603038	26.306052425596842	28.113880587945445	22.89021236042733
42-43	22.730114572134738	26.3199516010024	27.917867084000108	23.032066742862757
44-45	22.71353363290462	26.422233223518354	27.803677108320013	23.06055603525701
46-47	22.972043919081624	26.59804591102524	27.61163264244612	22.818277527447016
48-49	22.976537928216313	26.571501533344016	27.35708223614545	23.09487830229422
50-51	22.858602115129433	26.66311163167076	27.373139370816595	23.105146882383202
52-53	22.986668143502715	26.658584199820734	27.308785348502205	23.045962308174346
54-55	22.90617633237304	26.92656628534737	27.126940424633762	23.040316957645828
56-57	22.823638405059032	26.651704259073718	27.255457147737683	23.269200188129563
58-59	22.911866537995834	26.270902339728313	27.55537975731095	23.2618513649649
60-61	22.967277409233954	26.634706094709976	27.41593286594628	22.982083630109788
62-63	22.6737282749376	26.54747735046686	27.48739204878124	23.291402325814307
64-65	22.714858369396012	26.583266307098175	27.36023346296015	23.341641860545664
66-67	22.634435053418766	26.497372446362654	27.304886539014518	23.563305961204062
68-69	22.78553063622132	26.61918781653853	27.34172517339153	23.25355637384862
70-71	22.908144072411424	26.44151303548029	27.279188642612905	23.37115424949538
72-73	22.768988468280558	26.51207097597254	27.305417868488753	23.413522687258155
74-75	22.69491886618549	26.775878540765103	27.14876408753562	23.38043850551379
76-77	22.77866090561051	26.52299864512236	27.086452642660824	23.611887806606312
78-79	22.838959869760632	26.726898886141694	27.088936809445798	23.34520443465188
80-81	22.77073786476889	26.59438315321183	27.208685181995996	23.42619380002328
82-83	22.677725058637066	26.621137629428176	27.301848328824086	23.399288983110672
84-85	22.59828804091615	26.61350027034984	27.130147470545335	23.658064218188677
86-87	22.82453882990449	26.63602682301197	27.081878399764094	23.45755594731945
88-89	22.462758518424803	26.71600889126956	27.030628410427603	23.790604179878034
90-91	22.488101228313063	26.49548823207391	27.17049758849636	23.84591295111666
92-93	22.65650743926568	26.700130413846285	27.06849781625358	23.57486433063446
94-95	22.416021712876365	26.478214364143348	27.22556862447033	23.880195298509957
96-97	22.45305698584569	26.449540506558222	27.214482987949083	23.882919519647004
98-99	22.356698315724913	26.615406817676167	27.066042103870647	23.961852762728274
100	22.41296370769891	26.340805315404936	26.99479215773807	24.251438819158086
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	56.5
2	208.0
3	391.0
4	261.5
5	158.0
6	486.5
7	1180.5
8	2038.5
9	2355.0
10	1851.5
11	1360.5
12	1350.5
13	1539.0
14	1787.0
15	2011.5
16	2278.0
17	2616.0
18	2989.0
19	3486.5
20	4038.5
21	4660.5
22	5785.0
23	7892.5
24	10560.0
25	13683.0
26	18208.5
27	23798.5
28	30891.0
29	39228.0
30	46627.0
31	53978.0
32	61640.5
33	70439.5
34	80014.5
35	88747.5
36	97290.5
37	105884.0
38	113206.5
39	118399.5
40	124311.0
41	131926.5
42	137858.0
43	141521.5
44	143637.0
45	145917.0
46	149303.0
47	151128.0
48	155134.5
49	154481.0
50	148274.0
51	144270.5
52	137018.5
53	132517.5
54	129388.5
55	122391.5
56	112518.5
57	102047.0
58	94083.0
59	87523.0
60	80886.5
61	71610.5
62	63226.0
63	55232.5
64	46311.5
65	38377.5
66	30999.5
67	24090.0
68	18979.5
69	15128.0
70	11195.5
71	8283.5
72	6563.0
73	5222.0
74	4107.0
75	2993.5
76	1922.0
77	1308.0
78	964.5
79	683.5
80	444.0
81	204.0
82	84.0
83	40.5
84	21.5
85	11.5
86	9.5
87	7.5
88	6.5
89	4.5
90	3.0
91	1.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.0889210443777814E-4
2	3.6078715097451013E-4
3	1.9241981385307208E-4
4	0.012050290842548638
5	5.291544880959482E-4
6	0.0033432942656971276
7	8.899416390704584E-4
8	0.13928789275289255
9	0.0012988337435082364
10-11	0.0
12-13	1.5634109875562106E-4
14-15	2.0444605221888907E-4
16-17	0.0
18-19	1.2026238365817005E-4
20-21	0.0
22-23	2.7660348241379115E-4
24-25	3.367346742428761E-4
26-27	0.006397958810614647
28-29	0.008129737135292296
30-31	0.014684037044662562
32-33	0.017269678293313218
34-35	0.025868438724872378
36-37	0.03746173250951997
38-39	0.04260896253008965
40-41	0.05432251869839541
42-43	0.07082251773629634
44-45	0.10895771959430207
46-47	0.11660640719496167
48-49	0.11363592631860489
50-51	0.11498286501557638
52-53	0.10952295279749547
54-55	0.11652222352640097
56-57	0.09754481938514173
58-59	0.09198869726013427
60-61	0.0942015251194446
62-63	0.09255393046332767
64-65	0.0
66-67	3.607871509745102E-5
68-69	1.3228862202398705E-4
70-71	0.0
72-73	0.0
74-75	0.0
76-77	1.2026238365817005E-4
78-79	1.2026238365817005E-5
80-81	2.8862972077960814E-4
82-83	2.284985289505231E-4
84-85	0.0
86-87	6.373906333883013E-4
88-89	3.4876091260869314E-4
90-91	9.620990692653604E-5
92-93	0.0010102040227286284
94-95	0.0010342564994602623
96-97	2.0444605221888907E-4
98-99	2.405247673163401E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4157576.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.6407254685744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.63168829561116	31.543910067717967
2	16.396473971887577	15.294868823508681
3	6.317207978271055	8.839174891272846
4	3.140386071405732	5.858795384870785
5	1.7435509580490423	4.066024078741763
6	1.076310978792117	3.0119954928393438
7	0.7189602667048669	2.347297989553631
8	0.5093006947705198	1.9003323108596815
9	0.37585036527578075	1.5776940333681988
>10	1.9069453626470536	15.924417002122068
>50	0.127380903375274	4.090134705523692
>100	0.05398247262933995	4.274019230908997
>500	0.0012905793913610283	0.40686029191263257
>1k	5.162316840510203E-4	0.41198873796624075
>5k	1.5486950521529408E-4	0.45248695883345225
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7630	0.1835203974623675	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6113	0.1470327902604787	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	5050	0.12146500749475174	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.405247673163401E-5	0.0
10-11	0.0	0.0	0.0	2.405247673163401E-5	0.0
12-13	0.0	0.0	0.0	2.405247673163401E-5	0.0
14-15	0.0	0.0	0.0	2.405247673163401E-5	0.0
16-17	0.0	0.0	0.0	4.810495346326802E-5	0.0
18-19	0.0	0.0	0.0	4.810495346326802E-5	0.0
20-21	0.0	0.0	0.0	7.215743019490203E-5	0.0
22-23	0.0	0.0	0.0	1.4431486038980407E-4	0.0
24-25	0.0	0.0	0.0	2.2849852895052308E-4	0.0
26-27	0.0	0.0	0.0	3.4876091260869314E-4	0.0
28-29	0.0	0.0	0.0	0.0013950436504347726	0.0
30-31	0.0	0.0	0.0	0.005880830560884516	0.0
32-33	0.0	0.0	0.0	0.014864430620149818	0.0
34-35	0.0	0.0	0.0	0.027936951723792902	0.0
36-37	0.0	0.0	0.0	0.05103935562452737	0.0
38-39	0.0	0.0	0.0	0.09308308495142362	0.0
40-41	0.0	0.0	0.0	0.14456741139548623	0.0
42-43	0.0	0.0	0.0	0.1973144928679596	0.0
44-45	0.0	0.0	0.0	0.25789065551657986	0.0
46-47	0.0	0.0	0.0	0.33520734197041735	0.0
48-49	0.0	0.0	0.0	0.42172410077410494	0.0
50-51	0.0	0.0	0.0	0.5192809463976125	0.0
52-53	0.0	0.0	0.0	0.6206260571063524	0.0
54-55	0.0	0.0	0.0	0.7232579753202346	0.0
56-57	0.0	0.0	0.0	0.851914673357745	0.0
58-59	0.0	0.0	0.0	0.9969631342878639	0.0
60-61	0.0	0.0	0.0	1.1555771920946243	0.0
62-63	0.0	0.0	0.0	1.3133494132157777	0.0
64-65	0.0	0.0	0.0	1.4802254967798545	0.0
66-67	0.0	0.0	0.0	1.6722484447668546	0.0
68-69	0.0	0.0	0.0	1.896393475428952	0.0
70-71	0.0	0.0	0.0	2.1355833302866865	0.0
72-73	0.0	0.0	0.0	2.3799083889266246	0.0
74-75	0.0	0.0	0.0	2.6273362170649435	0.0
76-77	0.0	0.0	0.0	2.8944750498848366	0.0
78-79	0.0	0.0	0.0	3.1968507611165737	0.0
80-81	0.0	0.0	0.0	3.5319017619882356	0.0
82-83	0.0	0.0	0.0	3.8753230247625057	0.0
84-85	0.0	0.0	0.0	4.21120383608141	0.0
86-87	0.0	0.0	0.0	4.566025972826473	0.0
88	0.0	0.0	0.0	4.856748259081734	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	14915	0.0	28.504128	1
GTATCAA	15395	0.0	27.890026	1
TACATGG	14940	0.0	27.647024	2
ACATGGG	14975	0.0	26.639954	3
TCAACGC	16925	0.0	25.04382	4
CAACGCA	17235	0.0	24.618586	5
ATCAACG	17280	0.0	24.556545	3
GGTATCA	11010	0.0	24.235023	1
AACGCAG	17645	0.0	24.046547	6
TATCAAC	18300	0.0	23.26494	2
GAGTACA	10255	0.0	22.171349	1
TATACCG	200	5.922102E-7	21.168026	5
ACGCAGA	20125	0.0	20.989546	7
CATGGGG	10260	0.0	20.862602	4
AGTACAT	10720	0.0	20.230686	2
CGCAGAG	20900	0.0	20.143705	8
AGAGTAC	15650	0.0	19.614258	10-11
GAGTACT	10485	0.0	19.398142	12-13
GCAGAGT	22260	0.0	18.611614	9
CTACACT	2000	0.0	18.34717	4
>>END_MODULE