FastQCFastQC Report
Wed 25 May 2016
SRR1032921_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032921_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037227
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12950.035.930741
GTACATG32250.026.5242081
GTATCAA17900.025.7320161
CAACGCA19000.024.4896376
GGACCGT1351.4571862E-524.3705336
TCAACGC19300.024.1089695
TACATGG34600.023.9076732
CGCAGTA1000.001758494623.5001582
ATCAACG19600.023.5001564
CCGGTCG1000.001758988423.4990239
ACATGGG34300.023.2946153
GTACCTG8600.022.9536421
TACCTGG8050.022.7703382
AACGCAG20300.022.6898087
CAGCGTT1954.6750756E-721.6914069
ACCGAGC1754.4372773E-621.4858578
TATCAAC21800.021.128583
ACACCGT1605.4119246E-520.5626376
ACGCAGA22750.020.246298
CGCAGAG22800.020.2009149
Produced by FastQC (version 0.11.5)