##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032921_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1037227 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.29532301029572 33.0 31.0 34.0 28.0 34.0 2 31.431614294652956 34.0 31.0 34.0 28.0 34.0 3 31.49818410049102 34.0 31.0 34.0 28.0 34.0 4 34.82667535650344 37.0 35.0 37.0 32.0 37.0 5 34.88546480182255 37.0 35.0 37.0 32.0 37.0 6 34.9519285556585 37.0 35.0 37.0 32.0 37.0 7 34.86260866714808 37.0 36.0 37.0 32.0 37.0 8 34.84454801118752 37.0 35.0 37.0 32.0 37.0 9 36.44775348115697 39.0 38.0 39.0 33.0 39.0 10-11 36.43804152803581 39.0 38.0 39.0 32.5 39.0 12-13 36.37123696162942 39.0 38.0 39.0 32.0 39.0 14-15 37.811901830553964 41.0 39.0 41.0 33.0 41.0 16-17 37.84351737854877 41.0 39.0 41.0 33.0 41.0 18-19 37.86421776525293 41.0 39.0 41.0 33.0 41.0 20-21 37.90704783041706 41.0 39.0 41.0 33.0 41.0 22-23 37.903120049902284 41.0 39.0 41.0 32.5 41.0 24-25 37.94944790291807 41.0 39.0 41.0 33.0 41.0 26-27 37.9426923903832 41.0 39.0 41.0 32.5 41.0 28-29 37.936002919322384 41.0 39.0 41.0 32.0 41.0 30-31 37.89576823588279 41.0 39.0 41.0 32.0 41.0 32-33 37.850744822493056 41.0 39.0 41.0 32.0 41.0 34-35 37.78171605636953 41.0 38.0 41.0 32.0 41.0 36-37 37.693105752164186 41.0 38.0 41.0 32.0 41.0 38-39 37.593393249500835 40.5 38.0 41.0 31.0 41.0 40-41 37.508949824869575 40.0 38.0 41.0 31.0 41.0 42-43 37.40142755635941 40.0 38.0 41.0 31.0 41.0 44-45 37.26492175772516 40.0 38.0 41.0 30.5 41.0 46-47 37.15127112965629 40.0 38.0 41.0 30.0 41.0 48-49 37.02594755053619 40.0 37.5 41.0 30.0 41.0 50-51 36.89780347021433 40.0 37.0 41.0 30.0 41.0 52-53 36.75111330499495 40.0 37.0 41.0 30.0 41.0 54-55 36.594930521476975 40.0 36.0 41.0 30.0 41.0 56-57 36.423570250292364 40.0 36.0 41.0 30.0 41.0 58-59 36.22519515978662 39.5 35.5 41.0 30.0 41.0 60-61 35.998916823414746 39.0 35.0 41.0 29.0 41.0 62-63 35.753901508541524 39.0 35.0 41.0 29.0 41.0 64-65 35.493335113721486 38.5 35.0 40.5 29.0 41.0 66-67 35.20959973082074 38.0 35.0 40.0 28.5 41.0 68-69 34.908123294129446 37.0 34.5 40.0 28.0 41.0 70-71 34.585060454461754 37.0 34.0 40.0 28.0 41.0 72-73 34.3027630402988 36.0 34.0 39.0 28.0 41.0 74-75 34.03285298203768 36.0 34.0 39.0 28.0 41.0 76-77 33.71295579463319 35.5 34.0 39.0 27.5 40.0 78-79 33.34802989123885 35.0 34.0 37.5 27.0 39.5 80-81 33.024546699999135 35.0 34.0 37.0 27.0 39.0 82-83 32.70078439917202 35.0 34.0 36.5 26.0 39.0 84-85 32.52414900499119 35.0 34.0 36.0 26.0 38.0 86-87 32.28229018334463 35.0 34.0 36.0 26.0 37.0 88-89 32.04432057784844 35.0 33.0 36.0 26.0 37.0 90-91 31.844106931269625 35.0 33.0 35.0 25.5 36.5 92-93 31.64997584906679 35.0 33.0 35.0 25.0 36.0 94-95 31.4692526322589 35.0 33.0 35.0 25.0 36.0 96-97 31.28643392430008 35.0 33.0 35.0 24.5 36.0 98-99 31.11181496432314 35.0 33.0 35.0 23.5 36.0 100 30.9839379422248 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.027668379853484026 1101 2 0.13010494153005325 1101 3 0.2027855882050993 1101 4 0.041932524152073825 1101 5 0.07443756237181276 1101 6 0.18001686539278694 1101 7 0.24034463235366843 1101 8 0.22508294732251244 1101 9 0.2767681738103889 1101 10-11 0.26110264884458445 1101 12-13 0.16611319223553522 1101 14-15 0.1166747341842651 1101 16-17 0.12438018349818236 1101 18-19 0.056214766936911076 1101 20-21 0.13431332419395403 1101 22-23 0.05654140869553004 1101 24-25 0.01710005279024074 1101 26-27 0.03682155063256687 1101 28-29 0.1359884913763807 1101 30-31 0.18505647059196662 1101 32-33 0.06974665485688547 1101 34-35 0.05510801272266974 1101 36-37 -0.033322224638027365 1101 38-39 0.007592412658205205 1101 40-41 -0.03219352871078485 1101 42-43 -0.04842955750154232 1101 44-45 -0.007633488219994433 1101 46-47 -0.028567456727600415 1101 48-49 0.040552754475612574 1101 50-51 -0.01714537610659761 1101 52-53 -0.09419210269345513 1101 54-55 -0.051231009867613864 1101 56-57 0.09480387811067459 1101 58-59 0.026010208595337758 1101 60-61 0.09701095885922228 1101 62-63 0.03927286305153643 1101 64-65 -0.06776412388190067 1101 66-67 0.047246558910750025 1101 68-69 0.032406885525894324 1101 70-71 0.027214382249141522 1101 72-73 -0.08889466902224541 1101 74-75 -0.036070826061475714 1101 76-77 -0.06562794303086861 1101 78-79 -0.0424438649305543 1101 80-81 -0.07470194327173374 1101 82-83 -0.08096669023700542 1101 84-85 -0.12610229335609802 1101 86-87 -0.07855044825662816 1101 88-89 -0.11923607267232939 1101 90-91 0.007423627475787953 1101 92-93 -0.1411428597828568 1101 94-95 0.01744727227619336 1101 96-97 -0.05057165421395027 1101 98-99 0.017106294417928325 1101 100 0.11067265350006039 1102 1 0.3551726847123575 1102 2 0.3254294904557078 1102 3 0.2856372764584485 1102 4 0.3404875889121257 1102 5 0.23465317965114707 1102 6 0.3644332762634903 1102 7 0.33016457695200074 1102 8 0.3212347778233706 1102 9 0.3141811407939983 1102 10-11 0.3559536632939313 1102 12-13 0.2078375689668377 1102 14-15 0.30846742715424824 1102 16-17 0.2603734828917581 1102 18-19 0.20321914666289587 1102 20-21 0.2638848971519536 1102 22-23 0.3619599158453397 1102 24-25 0.32858279592117867 1102 26-27 0.34714853273932533 1102 28-29 0.3553500620883625 1102 30-31 0.3087097984350464 1102 32-33 0.25659811850891145 1102 34-35 0.321226901694736 1102 36-37 0.3634519387220081 1102 38-39 0.3526567983735589 1102 40-41 0.2515612440675383 1102 42-43 0.3186111327732206 1102 44-45 0.32185760259381624 1102 46-47 0.28892150037354725 1102 48-49 0.2824465180452762 1102 50-51 0.29281756467352693 1102 52-53 0.349650077436813 1102 54-55 0.1818518305905812 1102 56-57 0.19113726135541498 1102 58-59 0.24531562897416848 1102 60-61 0.24079043778413478 1102 62-63 0.15992308390951848 1102 64-65 0.24293719377925527 1102 66-67 0.1700819635674904 1102 68-69 0.20925797484236597 1102 70-71 0.18998203217053344 1102 72-73 0.15485111970276932 1102 74-75 0.17814175909985863 1102 76-77 0.13698959833320856 1102 78-79 0.21021859670005227 1102 80-81 0.29547006391550923 1102 82-83 0.2889882023286816 1102 84-85 0.25721637850135437 1102 86-87 0.21761822734418246 1102 88-89 0.19591703056941867 1102 90-91 0.09212453336783 1102 92-93 0.2214235847780479 1102 94-95 0.2849951872271532 1102 96-97 0.2119854319580945 1102 98-99 0.245094427980856 1102 100 0.11447777441044593 1103 1 0.11342736796704145 1103 2 0.23551100053721896 1103 3 0.2775613433825157 1103 4 0.25303227645681403 1103 5 0.2682224632204253 1103 6 0.2298343916646033 1103 7 0.21402701081444064 1103 8 0.12243597902457282 1103 9 0.11578274239559505 1103 10-11 0.12680308414335428 1103 12-13 0.16848571711499005 1103 14-15 0.20882671501353656 1103 16-17 0.19702799454626785 1103 18-19 0.23398741493117114 1103 20-21 0.23495418072123186 1103 22-23 0.15066469205012112 1103 24-25 0.20047790531629062 1103 26-27 0.22989377798457156 1103 28-29 0.19949063122893307 1103 30-31 0.224270001495249 1103 32-33 0.2692750811858744 1103 34-35 0.2720062524740925 1103 36-37 0.18624258651265535 1103 38-39 0.09601980423656187 1103 40-41 0.15609792360421437 1103 42-43 0.09260834427043108 1103 44-45 0.00834945158566569 1103 46-47 0.12163993309196997 1103 48-49 0.09916144726020804 1103 50-51 0.1017675861235432 1103 52-53 0.08783826562500252 1103 54-55 0.06446301320175962 1103 56-57 0.10610222632038102 1103 58-59 0.1702065913651296 1103 60-61 0.11343451042820618 1103 62-63 0.21659940308583714 1103 64-65 0.05670274504480233 1103 66-67 0.0703608138463423 1103 68-69 0.13397018705457953 1103 70-71 0.12419660388511033 1103 72-73 0.13414648649813188 1103 74-75 0.1758698443279414 1103 76-77 0.08960113844474193 1103 78-79 0.1341425206239748 1103 80-81 0.02873189717734448 1103 82-83 0.02544608878656618 1103 84-85 0.03136374014870569 1103 86-87 0.0969421941681432 1103 88-89 0.11252113467352842 1103 90-91 0.12350055224384704 1103 92-93 0.05537181872627883 1103 94-95 -0.0246966474646797 1103 96-97 -0.11303959306693301 1103 98-99 -0.2525746696882436 1103 100 -0.31755068261801256 1104 1 -0.4546864827555055 1104 2 -0.305959606150779 1104 3 -0.32499371025949486 1104 4 -0.4112999693971702 1104 5 -0.45299593191101195 1104 6 -0.5395833886227379 1104 7 -0.49898562067645713 1104 8 -0.4432205866319947 1104 9 -0.6018098501969931 1104 10-11 -0.45074255861968027 1104 12-13 -0.5002487287933732 1104 14-15 -0.4268742386221973 1104 16-17 -0.3692703407738023 1104 18-19 -0.2469450986100412 1104 20-21 -0.44640914194643244 1104 22-23 -0.3313696785712068 1104 24-25 -0.35865770544975817 1104 26-27 -0.39753997988396605 1104 28-29 -0.42250442283133793 1104 30-31 -0.3901244607252963 1104 32-33 -0.4037204015922242 1104 34-35 -0.36676761456064355 1104 36-37 -0.3401823774123045 1104 38-39 -0.3483135395533026 1104 40-41 -0.36781747259379216 1104 42-43 -0.33150860158564655 1104 44-45 -0.38091587246227476 1104 46-47 -0.44154889096641625 1104 48-49 -0.43708220039648893 1104 50-51 -0.4395187241845022 1104 52-53 -0.42650809654745103 1104 54-55 -0.4089891586199812 1104 56-57 -0.4581087237601267 1104 58-59 -0.4379531635337486 1104 60-61 -0.30288055534338554 1104 62-63 -0.31604458336249763 1104 64-65 -0.4673577355374192 1104 66-67 -0.42168307221928814 1104 68-69 -0.289604326193853 1104 70-71 -0.295829223749422 1104 72-73 -0.30818835257584 1104 74-75 -0.39105980412344366 1104 76-77 -0.48228229213433593 1104 78-79 -0.4299128885401373 1104 80-81 -0.48783255688711336 1104 82-83 -0.45026730812247706 1104 84-85 -0.29975803662523504 1104 86-87 -0.3355883389058647 1104 88-89 -0.32999277251429504 1104 90-91 -0.3736252084688694 1104 92-93 -0.5171442320506401 1104 94-95 -0.4407058596695421 1104 96-97 -0.2694588581818458 1104 98-99 -0.17877900078387654 1104 100 -0.3213882299338202 1105 1 -0.17809265884710257 1105 2 -0.23575955896863476 1105 3 -0.1499057973199207 1105 4 -0.07254111529304907 1105 5 -0.2816949396381503 1105 6 -0.1235556823137074 1105 7 -0.2152297346187808 1105 8 -0.11547970006757424 1105 9 -0.22764479956135375 1105 10-11 -0.14937661850693473 1105 12-13 -0.013356677962704566 1105 14-15 -0.04001961839161794 1105 16-17 -0.05818969081847314 1105 18-19 -0.07192127333046727 1105 20-21 -0.10067750785162843 1105 22-23 -0.08872187454232972 1105 24-25 -0.11560429679532547 1105 26-27 -0.14503140576414353 1105 28-29 -0.12631169824986443 1105 30-31 -0.054202071094472615 1105 32-33 -0.023576674901185868 1105 34-35 -0.06957482440110141 1105 36-37 -0.11842864095268624 1105 38-39 -0.1252612295444635 1105 40-41 -0.05475372474743523 1105 42-43 -0.08605286380254284 1105 44-45 -0.185699963051988 1105 46-47 -0.23471884973739066 1105 48-49 -0.10091666090613671 1105 50-51 -0.03565340791509186 1105 52-53 -0.04071400998610386 1105 54-55 -0.08168155573692815 1105 56-57 -0.050224834418436615 1105 58-59 -0.07947319507935902 1105 60-61 -0.09485243682933486 1105 62-63 -0.06258342389111249 1105 64-65 -0.08333532146384925 1105 66-67 -0.04974171434442809 1105 68-69 -0.030489400199130046 1105 70-71 -0.023774977910008488 1105 72-73 -0.07585674556392519 1105 74-75 -0.10817155000756884 1105 76-77 -0.18767387412438552 1105 78-79 -0.1201953256256374 1105 80-81 -0.14796164348678786 1105 82-83 -0.21315041819228497 1105 84-85 -0.31629840163107303 1105 86-87 -0.24833330919559415 1105 88-89 -0.28812138729252723 1105 90-91 -0.24649424054506142 1105 92-93 -0.273197215725105 1105 94-95 -0.3004528901326893 1105 96-97 -0.27723433079107807 1105 98-99 -0.203795970174248 1105 100 -0.23824545625396 1106 1 -0.027412018635057933 1106 2 0.01871430068967328 1106 3 -0.08349371894373192 1106 4 -0.00570012422473809 1106 5 -0.053138849666311216 1106 6 -0.15383075047511596 1106 7 0.10745585127717305 1106 8 -0.04485843403254819 1106 9 -0.058495349255373696 1106 10-11 0.01293437281701415 1106 12-13 -0.10984133875444968 1106 14-15 0.04629084379121906 1106 16-17 -0.016030251350962033 1106 18-19 -0.12288640804434436 1106 20-21 -0.0666331208660722 1106 22-23 0.008743563900175388 1106 24-25 0.0062744706467583455 1106 26-27 0.04988750052066848 1106 28-29 0.07061766019494087 1106 30-31 -0.07377501688875299 1106 32-33 -0.0029762864044826642 1106 34-35 -0.025321341463673264 1106 36-37 0.050004805147757736 1106 38-39 -0.00565829638220805 1106 40-41 0.09153532832127809 1106 42-43 -0.02128278656984861 1106 44-45 -0.15703930278614564 1106 46-47 -0.022539961087915117 1106 48-49 -0.09260947014630716 1106 50-51 -0.07373797391591097 1106 52-53 0.04452376468338315 1106 54-55 -0.07486866108754242 1106 56-57 -0.03678854580767421 1106 58-59 -0.045435503514255515 1106 60-61 0.015321112526670788 1106 62-63 0.02013159750785576 1106 64-65 0.1305750434933728 1106 66-67 0.14242273654385684 1106 68-69 0.09767859885620567 1106 70-71 0.0982460164938388 1106 72-73 0.07434243305001331 1106 74-75 0.06674286020096076 1106 76-77 0.034135260944971435 1106 78-79 -0.05728089431639205 1106 80-81 0.12292188013851302 1106 82-83 0.10749590494656047 1106 84-85 0.059971039942453785 1106 86-87 0.07867589518151163 1106 88-89 0.06453776995287086 1106 90-91 0.043087360601841596 1106 92-93 -0.017805474789994946 1106 94-95 -0.0910976428910999 1106 96-97 -0.12606533066893988 1106 98-99 -0.0819565636887809 1106 100 -0.12335285282696162 1107 1 -0.06354662173871617 1107 2 0.058239432659966894 1107 3 -0.06462330550794348 1107 4 -0.01462048578803632 1107 5 0.009692433985826199 1107 6 -0.04905685168893825 1107 7 -0.025305138746389844 1107 8 0.04943776449360371 1107 9 -0.15145962357977538 1107 10-11 -0.12244643024830992 1107 12-13 -0.11980458183661824 1107 14-15 -0.11341978762542482 1107 16-17 -0.017427355647647857 1107 18-19 0.0032613241927137437 1107 20-21 -0.005961509823627864 1107 22-23 -0.0115918497645211 1107 24-25 0.1370876233691476 1107 26-27 0.05919836068841278 1107 28-29 0.06527347437580744 1107 30-31 0.14979697646598567 1107 32-33 0.04756544553927 1107 34-35 0.053956664832419676 1107 36-37 0.03461628910453385 1107 38-39 0.018373565820866133 1107 40-41 -0.107620511854023 1107 42-43 -0.05982362151134879 1107 44-45 -0.07960942376505642 1107 46-47 -0.06847000732699371 1107 48-49 0.10030556992781214 1107 50-51 0.09050907571484856 1107 52-53 -0.019335657370923798 1107 54-55 0.05897866385152639 1107 56-57 0.01740676324296686 1107 58-59 0.08236462172464343 1107 60-61 0.10927045983337536 1107 62-63 -0.02821709582522658 1107 64-65 0.026776518270615668 1107 66-67 0.008782979168998395 1107 68-69 0.03307115761722201 1107 70-71 -0.010487767047720808 1107 72-73 -0.004318108239637297 1107 74-75 0.032223792307355836 1107 76-77 -0.10422035389343165 1107 78-79 -0.0017276666213703606 1107 80-81 0.020660906797942857 1107 82-83 0.010912583446511803 1107 84-85 0.004463981481336532 1107 86-87 -0.026525360000896114 1107 88-89 0.10602878014346118 1107 90-91 0.06349726580360127 1107 92-93 0.16348752758364782 1107 94-95 0.15255816120236432 1107 96-97 0.14271740002009992 1107 98-99 0.2255169549577687 1107 100 0.18579788933377372 1108 1 0.17764361933413042 1108 2 0.3353159131912946 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2214 42-43 0.20708619167442777 2214 44-45 0.3469598355874908 2214 46-47 0.3276769965693518 2214 48-49 0.39513954144068464 2214 50-51 0.42146156125200207 2214 52-53 0.47921548404275427 2214 54-55 0.5262976714897789 2214 56-57 0.4896879914303014 2214 58-59 0.480413810164805 2214 60-61 0.3805313099722625 2214 62-63 0.4162123826474584 2214 64-65 0.4033730252242478 2214 66-67 0.4837295992653736 2214 68-69 0.3705992717194775 2214 70-71 0.4348878277495629 2214 72-73 0.3752922785184438 2214 74-75 0.4368664540891274 2214 76-77 0.3644572589421742 2214 78-79 0.47990079277730757 2214 80-81 0.3210825239602215 2214 82-83 0.4523923546764763 2214 84-85 0.4337063540404671 2214 86-87 0.43078358592874366 2214 88-89 0.4498581593717361 2214 90-91 0.45803086168393037 2214 92-93 0.5081816921358282 2214 94-95 0.4955630774382982 2214 96-97 0.5377151316744673 2214 98-99 0.5487678751648524 2214 100 0.606237982703135 2215 1 0.03291722029271682 2215 2 -0.10204724000609389 2215 3 0.0019453756023679603 2215 4 -0.09831614019011425 2215 5 -0.0563056896659333 2215 6 0.036179649502400935 2215 7 -0.02449269225750328 2215 8 0.04863830074928188 2215 9 -0.12392151409970609 2215 10-11 -0.08691009598773292 2215 12-13 -0.17362318282973632 2215 14-15 -0.2324144995485753 2215 16-17 -0.11777201335075205 2215 18-19 -0.025548240126873623 2215 20-21 0.006335370012870101 2215 22-23 0.02640010230045675 2215 24-25 0.03518828200428459 2215 26-27 0.04153004111337566 2215 28-29 0.017301379076997137 2215 30-31 -0.01990594417323166 2215 32-33 0.0058402711092711 2215 34-35 -0.02504026099033041 2215 36-37 -0.016686221826603287 2215 38-39 -0.04571694850764629 2215 40-41 -0.03200422438599304 2215 42-43 0.0010818497588616083 2215 44-45 -0.003278914520315368 2215 46-47 -0.0438224375942724 2215 48-49 0.02280217220689451 2215 50-51 0.007353742642536076 2215 52-53 -0.07108931867571755 2215 54-55 -0.0395555842869868 2215 56-57 -0.03879827119205714 2215 58-59 -0.07757237096606673 2215 60-61 6.206037710185797E-4 2215 62-63 -0.00391015231177505 2215 64-65 -0.05699464104064589 2215 66-67 -0.09681422366451642 2215 68-69 -0.06050880686471771 2215 70-71 -0.10820290649948561 2215 72-73 -0.11764021491543275 2215 74-75 -0.08665680812408283 2215 76-77 -0.029051629461754658 2215 78-79 0.017814438810823674 2215 80-81 -0.005082167420304984 2215 82-83 -0.01882165977222172 2215 84-85 0.1584663224751779 2215 86-87 0.05491522583520947 2215 88-89 0.04539150504389511 2215 90-91 0.06295506539239071 2215 92-93 0.10267498543243292 2215 94-95 0.11524353258744213 2215 96-97 0.18090733893970068 2215 98-99 0.15542995007010774 2215 100 0.18860899518345775 2216 1 0.5702648797110967 2216 2 0.5125285053584641 2216 3 0.45223869095705993 2216 4 0.5297778137163718 2216 5 0.587741080636242 2216 6 0.6243789696203947 2216 7 0.637641212466022 2216 8 0.5821971420567635 2216 9 0.6250203678715422 2216 10-11 0.6045233778683183 2216 12-13 0.6126272885930035 2216 14-15 0.6639659747672795 2216 16-17 0.5351145791281837 2216 18-19 0.5438480933666128 2216 20-21 0.6014938261744334 2216 22-23 0.6380444700256973 2216 24-25 0.5865204064288889 2216 26-27 0.5362714173061391 2216 28-29 0.5298354119241822 2216 30-31 0.6455941126558074 2216 32-33 0.5153392778925863 2216 34-35 0.6018596372340212 2216 36-37 0.5139665888861913 2216 38-39 0.5199291525033942 2216 40-41 0.5186646792784515 2216 42-43 0.6202655909697299 2216 44-45 0.6211850842811089 2216 46-47 0.6804281939830403 2216 48-49 0.6086463159740418 2216 50-51 0.7100693959440463 2216 52-53 0.6145559880202995 2216 54-55 0.6404872183147532 2216 56-57 0.7868416690341249 2216 58-59 0.6693984150312531 2216 60-61 0.7755009075997421 2216 62-63 0.68966070704667 2216 64-65 0.6534938368709433 2216 66-67 0.6626968915796141 2216 68-69 0.6844742542058384 2216 70-71 0.6042548914784334 2216 72-73 0.6431167802230959 2216 74-75 0.5829141132296911 2216 76-77 0.5221547284165595 2216 78-79 0.7385796095820965 2216 80-81 0.7805846761913315 2216 82-83 0.7203850197068107 2216 84-85 0.7584303845634821 2216 86-87 0.7415810336083908 2216 88-89 0.7226046410771261 2216 90-91 0.6434206308671975 2216 92-93 0.7954542743461204 2216 94-95 0.7732592690660027 2216 96-97 0.8025670182172497 2216 98-99 0.7208837687234677 2216 100 0.7043457966382825 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19809.0 3 3184.0 4 1967.0 5 1569.0 6 1242.0 7 1217.0 8 1413.0 9 1611.0 10 1662.0 11 1774.0 12 1966.0 13 2138.0 14 2121.0 15 2315.0 16 2316.0 17 2302.0 18 2198.0 19 2220.0 20 2140.0 21 2334.0 22 2672.0 23 3959.0 24 6403.0 25 8589.0 26 9273.0 27 7354.0 28 7324.0 29 9163.0 30 11192.0 31 12673.0 32 16435.0 33 22186.0 34 32074.0 35 49705.0 36 92950.0 37 195735.0 38 329783.0 39 161808.0 40 451.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0654067818034 20.465731562701723 13.112811670498875 23.356049984996012 2 15.063891952499088 20.296827643702617 41.18929830300118 23.44998210079712 3 17.595826884405625 26.126069621401303 30.804806879542397 25.473296614650675 4 11.548920123282146 17.110511983727065 40.32163748333047 31.018930409660317 5 11.865126386117405 39.02357123282913 36.24294156017091 12.86836082088256 6 29.593130519360216 39.49575047860186 18.178283054406226 12.732835947631694 7 26.44843490678915 33.63985487668378 22.909510208807752 17.002200007719324 8 24.67335223922938 37.823885692340795 20.192770665820113 17.309991402609707 9 24.563883504138996 17.101150851583856 21.378802601019142 36.95616304325801 10-11 23.252744198971794 27.665655442854277 29.400560418692972 19.681039939480957 12-13 23.65350639776838 25.10404032509405 29.3548583189322 21.887594958205366 14-15 21.13607921349995 27.090165951201723 26.169951630131155 25.603803205167168 16-17 20.899025472790427 30.607149749131608 26.731233114627557 21.762591663450408 18-19 20.99536138053806 29.566700356761217 28.43017828412087 21.007759978579855 20-21 22.13202304107448 28.72087301357571 28.034320394438495 21.112783550911317 22-23 21.810410914492557 28.53065491683339 28.04565105484601 21.61328311382804 24-25 21.65548277597209 28.66879296478679 27.99414118188533 21.681583077355793 26-27 21.424405211320945 28.5159410361372 28.223099727662408 21.83655402487945 28-29 21.415739053163662 28.61286846043335 28.265574210205884 21.7058182761971 30-31 21.63354332729629 29.03981072561293 27.89346368461731 21.433182262473476 32-33 21.286541187322644 28.70969578318775 28.34805900044288 21.655704029046728 34-35 21.539608223400943 28.465047169014866 28.39017495109426 21.60516965648993 36-37 21.840165601107223 28.74333130882667 27.93235478425979 21.484148305806315 38-39 21.49264632456128 29.004312297356034 28.0096231913702 21.49341818671249 40-41 21.38842991154669 28.60734883698492 28.35310889140288 21.651112360065515 42-43 21.33992044327905 28.650551083471836 28.253539405664498 21.755989067584615 44-45 21.43538786195158 28.699362480834377 28.289563192748474 21.57568646446557 46-47 21.476033097725132 28.92379321158903 28.061177719344798 21.538995971341038 48-49 21.286911830409917 28.757477416970396 28.217520821934468 21.738089930685227 50-51 21.39281494158648 28.61999535837719 28.195011847718103 21.792177852318233 52-53 21.365405381410845 28.66335157502236 28.212166508567737 21.75907653499905 54-55 21.541173189586875 29.073387315455733 27.96098310380799 21.424456391149402 56-57 21.301491079768713 28.69715795487004 28.10471139519983 21.896639570161422 58-59 21.269948794254997 28.316058088823848 28.450670383702935 21.963322733218213 60-61 21.582045856093963 28.790567474228745 28.02811655371991 21.599270115957385 62-63 21.37240758744304 28.745389323252766 28.108084636223936 21.774118453080256 64-65 21.329737318862428 28.560476487999747 28.27355120099003 21.83623499214779 66-67 21.323055456917448 28.541804974448443 28.14724182118541 21.987897747448702 68-69 21.63181987482945 28.705519199186373 27.943129032195813 21.719531893788368 70-71 21.55701160837872 28.49642041619567 28.125266858587743 21.821301116837866 72-73 21.28848364790221 28.68384754220398 28.232655371102183 21.795013438791624 74-75 21.415641695879177 28.696914521923134 28.13789656406827 21.749547218129415 76-77 21.439567877796954 28.575026281548112 28.041097152715693 21.944308687939245 78-79 21.491138773930906 28.67304020093229 28.110547498043655 21.72527352709315 80-81 21.42434989605381 28.476383782174715 28.236504243002024 21.862762078769453 82-83 21.316658279929193 28.59551014441431 28.303188520188723 21.784643055467775 84-85 21.382273265645317 28.568637241918328 28.10514559195656 21.9439439004798 86-87 21.523174973826535 28.530860564188913 28.13234847132498 21.81361599065957 88-89 21.340946260836844 28.507229386479093 28.132710018863467 22.0191143338206 90-91 21.2704278419274 28.48013237844605 28.193424978712834 22.05601480091372 92-93 21.4642434674545 28.574736643040605 28.020331408127547 21.94068848137735 94-95 21.226361617620597 28.421972292214527 28.278932309041622 22.072733781123258 96-97 21.415873361992485 28.319203963202693 28.22952149894663 22.035401175858194 98-99 21.341897886279266 28.413105406233342 28.18077220878148 22.064224498705908 100 21.377545703997868 28.331268597098564 28.106270237309587 22.184915461593977 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1421.0 1 962.0 2 418.0 3 294.0 4 233.5 5 177.0 6 156.5 7 166.0 8 155.5 9 170.0 10 174.5 11 160.0 12 160.5 13 171.5 14 183.0 15 215.0 16 259.0 17 346.0 18 500.0 19 693.5 20 1047.0 21 1523.0 22 2265.5 23 3612.5 24 5281.0 25 7119.0 26 9381.5 27 12014.0 28 15077.5 29 18110.0 30 20386.5 31 22853.5 32 25435.5 33 27622.5 34 29776.5 35 31346.0 36 32785.0 37 34304.0 38 35046.0 39 35100.0 40 35485.0 41 35828.5 42 35564.0 43 35399.5 44 34828.0 45 34115.5 46 33845.0 47 33278.5 48 32733.0 49 31802.5 50 30354.5 51 29267.5 52 27434.0 53 25587.5 54 24543.5 55 23584.0 56 21882.0 57 19493.0 58 17561.5 59 16156.0 60 14543.5 61 12650.5 62 10970.5 63 9321.0 64 7757.0 65 6331.0 66 5146.5 67 3981.0 68 2924.0 69 2189.0 70 1568.5 71 1145.0 72 902.0 73 671.5 74 522.5 75 387.0 76 278.0 77 227.0 78 154.0 79 106.0 80 82.0 81 58.0 82 44.5 83 31.0 84 22.5 85 14.5 86 13.0 87 11.5 88 8.5 89 6.5 90 3.0 91 3.5 92 5.0 93 3.0 94 2.0 95 3.0 96 2.0 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08059952160905953 2 0.08368467076155943 3 0.08474519078273127 4 0.08725187446913742 5 0.08474519078273127 6 0.08435954713866878 7 0.0835882598505438 8 0.08349184893952818 9 0.08329902711749694 10-11 0.08281697256241884 12-13 0.08194927436327824 14-15 0.08079234343109079 16-17 0.07973182340991894 18-19 0.07900874157730178 20-21 0.07876771429976273 22-23 0.0813226034416767 24-25 0.08103337070862984 26-27 0.079972850687458 28-29 0.07577897605827848 30-31 0.081756452541247 32-33 0.07982823432093457 34-35 0.07659846880191125 36-37 0.07332049782738012 38-39 0.07447742875956759 40-41 0.07756257791206746 42-43 0.08286517801792664 44-45 0.08349184893952818 46-47 0.08696264173609056 48-49 0.09062625635468417 50-51 0.09173498183136382 52-53 0.09216883093093411 54-55 0.09236165275296536 56-57 0.08975855815554358 58-59 0.08845701085683269 60-61 0.08657699809202807 62-63 0.08768572356870771 64-65 0.0862395599034734 66-67 0.08590212171491872 68-69 0.08493801260476251 70-71 0.08325082166198913 72-73 0.08305799983995789 74-75 0.08252773982937198 76-77 0.08228671255183291 78-79 0.08272056165140321 80-81 0.08421493077214535 82-83 0.08373287621706724 84-85 0.08238312346284854 86-87 0.08407031440562192 88-89 0.08021387796499706 90-91 0.07756257791206746 92-93 0.07722513972351279 94-95 0.07746616700105184 96-97 0.07640564697988 98-99 0.07563435969175504 100 0.07471845603710663 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1037227.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.78020564393822 #Duplication Level Percentage of deduplicated Percentage of total 1 84.93030252222734 64.36035790536273 2 10.183470819633737 15.434110257617773 3 2.4109261714003374 5.481014431832105 4 0.9214303087557524 2.7930471313627354 5 0.45064619709396464 1.7075030744219677 6 0.27253385817943554 1.2391603090664112 7 0.1781829239197326 0.9451917031812872 8 0.12337221157470309 0.7479337251102763 9 0.08808604322494638 0.600766062295254 >10 0.42129095767123964 5.4173456000391935 >50 0.016956696599751304 0.8449532379792297 >100 0.002546627934900559 0.26544924604572107 >500 2.5466178418979306E-4 0.16316731568531526 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.641091101562145E-5 0.0 10-11 0.0 0.0 0.0 9.641091101562145E-5 0.0 12-13 0.0 0.0 0.0 9.641091101562145E-5 0.0 14-15 0.0 0.0 0.0 1.928218220312429E-4 0.0 16-17 4.820545550781073E-5 0.0 0.0 2.8923273304686436E-4 0.0 18-19 9.641091101562145E-5 0.0 0.0 2.8923273304686436E-4 0.0 20-21 9.641091101562145E-5 0.0 0.0 2.8923273304686436E-4 0.0 22-23 9.641091101562145E-5 0.0 0.0 2.8923273304686436E-4 0.0 24-25 9.641091101562145E-5 0.0 0.0 3.374381885546751E-4 0.0 26-27 9.641091101562145E-5 0.0 0.0 6.266709216015394E-4 0.0 28-29 9.641091101562145E-5 0.0 0.0 0.0016871909427733755 0.0 30-31 9.641091101562145E-5 0.0 0.0 0.004001052807148291 0.0 32-33 9.641091101562145E-5 0.0 0.0 0.008050311069804392 0.0 34-35 9.641091101562145E-5 0.0 0.0 0.014991896662929136 0.0 36-37 9.641091101562145E-5 0.0 0.0 0.026850438717850575 0.0 38-39 9.641091101562145E-5 0.0 0.0 0.05061572828320127 0.0 40-41 9.641091101562145E-5 0.0 0.0 0.08002105614296581 0.0 42-43 9.641091101562145E-5 0.0 0.0 0.1096192058247616 0.0 44-45 9.641091101562145E-5 0.0 0.0 0.14389328469081503 0.0 46-47 9.641091101562145E-5 0.0 0.0 0.1864587019042119 0.0 48-49 9.641091101562145E-5 0.0 0.0 0.2417503593716708 0.0 50-51 9.641091101562145E-5 0.0 0.0 0.30653849157416846 0.0 52-53 9.641091101562145E-5 0.0 0.0 0.3666506945924084 0.0 54-55 9.641091101562145E-5 0.0 0.0 0.42806444490935924 0.0 56-57 9.641091101562145E-5 0.0 0.0 0.5023490518468956 0.0 58-59 9.641091101562145E-5 0.0 0.0 0.5881065571952909 0.0 60-61 9.641091101562145E-5 0.0 0.0 0.6916036701705606 0.0 62-63 9.641091101562145E-5 0.0 0.0 0.794907961323799 0.0 64-65 9.641091101562145E-5 0.0 0.0 0.9000922652418419 0.0 66-67 9.641091101562145E-5 0.0 0.0 1.0278367223375404 0.0 68-69 9.641091101562145E-5 0.0 0.0 1.1719710343058944 0.0 70-71 9.641091101562145E-5 0.0 0.0 1.331290064759209 0.0 72-73 9.641091101562145E-5 0.0 0.0 1.4971650371615857 0.0 74-75 9.641091101562145E-5 0.0 0.0 1.655230725771697 0.0 76-77 9.641091101562145E-5 0.0 0.0 1.8244800800596206 0.0 78-79 9.641091101562145E-5 0.0 0.0 2.0353307424507845 0.0 80-81 9.641091101562145E-5 0.0 0.0 2.270091310773823 0.0 82-83 9.641091101562145E-5 0.0 0.0 2.5147339974759624 0.0 84-85 9.641091101562145E-5 0.0 0.0 2.7452042802588057 0.0 86-87 9.641091101562145E-5 0.0 0.0 2.9776991921729765 0.0 88 9.641091101562145E-5 0.0 0.0 3.1743292451893366 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1295 0.0 35.93074 1 GTACATG 3225 0.0 26.524208 1 GTATCAA 1790 0.0 25.732016 1 CAACGCA 1900 0.0 24.489637 6 GGACCGT 135 1.4571862E-5 24.370533 6 TCAACGC 1930 0.0 24.108969 5 TACATGG 3460 0.0 23.907673 2 CGCAGTA 100 0.0017584946 23.500158 2 ATCAACG 1960 0.0 23.500156 4 CCGGTCG 100 0.0017589884 23.499023 9 ACATGGG 3430 0.0 23.294615 3 GTACCTG 860 0.0 22.953642 1 TACCTGG 805 0.0 22.770338 2 AACGCAG 2030 0.0 22.689808 7 CAGCGTT 195 4.6750756E-7 21.691406 9 ACCGAGC 175 4.4372773E-6 21.485857 8 TATCAAC 2180 0.0 21.12858 3 ACACCGT 160 5.4119246E-5 20.562637 6 ACGCAGA 2275 0.0 20.24629 8 CGCAGAG 2280 0.0 20.200914 9 >>END_MODULE