FastQCFastQC Report
Wed 25 May 2016
SRR1032921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032921_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037227
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32730.31555291175412903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28480.27457827457248996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17280.16659805423499388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.050.416441
GTATCAA36450.033.0617182
GTACATG30750.027.0441111
TCAACGC45550.026.3481945
ATCAACG46000.025.9931474
CAACGCA46250.025.8476496
TACCTGG7700.025.6768382
TATCAAC48800.025.0805173
AACGCAG47550.025.0420047
GTACCTG8000.024.6662921
TACATGG31700.024.6508452
ACATGGG30800.023.689943
ACGCAGA53700.021.911137
CGCAGAG54600.021.549968
ACCTGGG9100.021.2092573
GAGTACT40450.020.90329612-13
CTGTCGC2057.598301E-720.6228879
AGAGTAC51550.020.5028710-11
GTATAGA4600.020.427571
CAGAGTA57800.019.91127410-11
Produced by FastQC (version 0.11.5)