##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032921_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1037227 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.77910621300834 34.0 31.0 34.0 31.0 34.0 2 32.84225728794179 34.0 31.0 34.0 31.0 34.0 3 32.90601093106909 34.0 31.0 34.0 31.0 34.0 4 36.33791253023687 37.0 37.0 37.0 35.0 37.0 5 36.33167667251238 37.0 37.0 37.0 35.0 37.0 6 36.39762077153796 37.0 37.0 37.0 35.0 37.0 7 36.362442358326575 37.0 37.0 37.0 35.0 37.0 8 36.30379367293755 37.0 37.0 37.0 35.0 37.0 9 38.12792378139019 39.0 39.0 39.0 37.0 39.0 10-11 38.13676128754844 39.0 39.0 39.0 37.0 39.0 12-13 38.13853139187468 39.0 39.0 39.0 37.0 39.0 14-15 39.66470840037909 41.0 40.0 41.0 37.0 41.0 16-17 39.67378596970576 41.0 40.0 41.0 37.0 41.0 18-19 39.7131230675638 41.0 40.0 41.0 37.0 41.0 20-21 39.729938094554036 41.0 40.0 41.0 37.0 41.0 22-23 39.6878055623311 41.0 40.0 41.0 37.0 41.0 24-25 39.66834694816082 41.0 40.0 41.0 37.0 41.0 26-27 39.587208971613734 41.0 40.0 41.0 37.0 41.0 28-29 39.52016144971159 41.0 40.0 41.0 36.5 41.0 30-31 39.39511215963333 41.0 39.0 41.0 36.0 41.0 32-33 39.24593314674608 41.0 39.0 41.0 35.0 41.0 34-35 39.14048467693186 41.0 39.0 41.0 35.0 41.0 36-37 39.037212201379255 41.0 39.0 41.0 35.0 41.0 38-39 38.93246849532456 41.0 39.0 41.0 35.0 41.0 40-41 38.82532319347645 41.0 39.0 41.0 35.0 41.0 42-43 38.68050822047633 41.0 39.0 41.0 35.0 41.0 44-45 38.493329811121384 41.0 38.5 41.0 35.0 41.0 46-47 38.235768062343155 41.0 38.0 41.0 34.5 41.0 48-49 38.000577501356986 40.5 38.0 41.0 34.0 41.0 50-51 37.83442438347633 40.0 38.0 41.0 33.5 41.0 52-53 37.69227469011123 40.0 38.0 41.0 33.0 41.0 54-55 37.548838875193184 40.0 37.5 41.0 33.0 41.0 56-57 37.343686097643044 40.0 37.0 41.0 33.0 41.0 58-59 37.186534866523914 40.0 36.5 41.0 33.0 41.0 60-61 37.00355033179815 40.0 36.0 41.0 32.5 41.0 62-63 36.77684103865403 39.0 35.5 41.0 32.0 41.0 64-65 36.504055042917315 39.0 35.0 41.0 32.0 41.0 66-67 36.2418433959008 39.0 35.0 41.0 31.0 41.0 68-69 35.96314403693695 38.0 35.0 40.5 31.0 41.0 70-71 35.64334036811614 37.0 35.0 40.0 31.0 41.0 72-73 35.308024665767476 37.0 35.0 39.5 31.0 41.0 74-75 34.96744251740458 36.0 35.0 39.0 30.5 41.0 76-77 34.621607902609554 36.0 35.0 39.0 30.0 40.5 78-79 34.280099245391796 35.5 35.0 37.5 30.0 39.5 80-81 33.941682968144875 35.0 34.0 37.0 30.0 39.0 82-83 33.62728361294104 35.0 34.0 37.0 29.5 39.0 84-85 33.33358994704149 35.0 34.0 36.0 29.0 38.0 86-87 33.173576275974305 35.0 34.0 36.0 29.0 37.0 88-89 32.97960812821108 35.0 34.0 36.0 29.0 37.0 90-91 32.79162275953094 35.0 34.0 35.0 29.0 36.5 92-93 32.62029382189241 35.0 34.0 35.0 29.0 36.0 94-95 32.5112492250973 35.0 34.0 35.0 29.0 36.0 96-97 32.44363866347482 35.0 34.0 35.0 29.0 36.0 98-99 32.32827288529897 35.0 34.0 35.0 29.0 36.0 100 32.23618648569696 35.0 34.0 35.0 29.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22187512014466648 1101 2 0.22224179413117184 1101 3 0.14772357427719385 1101 4 0.12436909100890858 1101 5 0.14934057150461655 1101 6 0.19700615638306118 1101 7 0.17096251369088833 1101 8 0.05420585135743039 1101 9 0.07645034854918009 1101 10-11 0.1793397888235404 1101 12-13 0.16336470623434707 1101 14-15 0.2507937416374233 1101 16-17 0.23707017981234912 1101 18-19 0.228395124002013 1101 20-21 0.3011281613820458 1101 22-23 0.29878233890493533 1101 24-25 0.21642787082887338 1101 26-27 0.2847706317406704 1101 28-29 0.2483576115190047 1101 30-31 0.2961758400413359 1101 32-33 0.2609474894805359 1101 34-35 0.22824491204745812 1101 36-37 0.28700626252822303 1101 38-39 0.3476348541002352 1101 40-41 0.4188968394282355 1101 42-43 0.3942059474537629 1101 44-45 0.2871220579974434 1101 46-47 0.3257178598910144 1101 48-49 0.24863547353289306 1101 50-51 0.24986591111925804 1101 52-53 0.28492388759452325 1101 54-55 0.24070293134521847 1101 56-57 0.17195000372829838 1101 58-59 0.2323443871416373 1101 60-61 0.24653136436901235 1101 62-63 0.2631431452634132 1101 64-65 0.23487948305080408 1101 66-67 0.23794416153379672 1101 68-69 0.14680740958260685 1101 70-71 0.24540097378802983 1101 72-73 0.32626475910100794 1101 74-75 0.423922953338149 1101 76-77 0.406347377401886 1101 78-79 0.31599325909599685 1101 80-81 0.3648884398596195 1101 82-83 0.3657422707272602 1101 84-85 0.34008889303004963 1101 86-87 0.3601383842419992 1101 88-89 0.2628752323425658 1101 90-91 0.2293561854559769 1101 92-93 0.3503927595730687 1101 94-95 0.38689523204674003 1101 96-97 0.33193719218326834 1101 98-99 0.32646374981236903 1101 100 0.4299160213619828 1102 1 0.2673830934919721 1102 2 0.2670272998585048 1102 3 0.27385968268476546 1102 4 0.21039701644126296 1102 5 0.16124818548979647 1102 6 0.15675946925271234 1102 7 0.1577896455318708 1102 8 -0.03943605925124416 1102 9 0.1428668342869912 1102 10-11 0.18533290104934252 1102 12-13 0.23185688262126547 1102 14-15 0.30012087347840577 1102 16-17 0.2351311216405989 1102 18-19 0.23882317670845765 1102 20-21 0.2878542226233378 1102 22-23 0.30787027032666714 1102 24-25 0.19965576631438609 1102 26-27 0.26191651030622154 1102 28-29 0.2718733710458423 1102 30-31 0.3330478921488478 1102 32-33 0.30215715482454897 1102 34-35 0.28897149735853134 1102 36-37 0.28279779505798075 1102 38-39 0.31964016966510655 1102 40-41 0.2566504517663333 1102 42-43 0.2482150812412911 1102 44-45 0.2901747644435062 1102 46-47 0.27716747432539535 1102 48-49 0.3723945511700819 1102 50-51 0.43381627422475333 1102 52-53 0.47227789672831477 1102 54-55 0.48178625843962664 1102 56-57 0.4382429587331629 1102 58-59 0.38020131609275154 1102 60-61 0.3996636171203818 1102 62-63 0.4019879457425617 1102 64-65 0.536964532088767 1102 66-67 0.5579421026882443 1102 68-69 0.5265268450845966 1102 70-71 0.5435358468882825 1102 72-73 0.529474686479908 1102 74-75 0.5487638607275329 1102 76-77 0.4679208969047721 1102 78-79 0.43589948803963097 1102 80-81 0.4618357334135581 1102 82-83 0.4173977697165583 1102 84-85 0.36478848874764935 1102 86-87 0.4156467319248591 1102 88-89 0.42571307018040727 1102 90-91 0.3909044373089401 1102 92-93 0.42488459905798237 1102 94-95 0.4112644766375979 1102 96-97 0.4525144176081426 1102 98-99 0.42515638288865887 1102 100 0.43523158623433744 1103 1 0.16550979161866763 1103 2 0.19966708249828713 1103 3 0.1540863379114228 1103 4 -0.0036098475655990114 1103 5 0.021150945392555798 1103 6 0.037790500283747974 1103 7 0.059966822709050405 1103 8 0.03980031998514022 1103 9 0.018546084966246212 1103 10-11 0.12810424382067964 1103 12-13 0.12928645505083836 1103 14-15 0.12293297129050273 1103 16-17 0.16330572481520989 1103 18-19 0.15677326965855087 1103 20-21 0.17308052034964305 1103 22-23 0.09799253544893105 1103 24-25 0.10858791274653612 1103 26-27 0.1825138934036019 1103 28-29 0.10632031799278963 1103 30-31 0.09211946372041524 1103 32-33 0.0787998689672591 1103 34-35 -0.02323356484653516 1103 36-37 0.04785392261410948 1103 38-39 0.12961264213758739 1103 40-41 0.07647920909509054 1103 42-43 0.01967404020025043 1103 44-45 -0.007020645251905933 1103 46-47 0.13630518346310083 1103 48-49 0.08849279226833318 1103 50-51 0.09368507159354778 1103 52-53 0.0666204535708701 1103 54-55 0.06493547908884523 1103 56-57 0.039853497843708396 1103 58-59 -0.010521549630112759 1103 60-61 0.051227681184442986 1103 62-63 0.1348976411522571 1103 64-65 0.21808493820916652 1103 66-67 0.07562407037021046 1103 68-69 0.05081541937317269 1103 70-71 0.14987432822676539 1103 72-73 0.028334260339484274 1103 74-75 -0.10411222964856393 1103 76-77 -0.1142148102309335 1103 78-79 -0.0746342599941201 1103 80-81 -0.15346885689103118 1103 82-83 -0.24377769145890937 1103 84-85 -0.15771079625162798 1103 86-87 -0.1333147295366004 1103 88-89 -0.12006127559394031 1103 90-91 -0.06969187328736837 1103 92-93 -0.04847447430109497 1103 94-95 -0.08248820461508899 1103 96-97 -0.1200599424662201 1103 98-99 0.032306390038669974 1103 100 0.04593157193431807 1104 1 0.12086562740928741 1104 2 0.16124978321142436 1104 3 0.09715313423908611 1104 4 0.07938626260442305 1104 5 0.057299177192959405 1104 6 0.10719834686550911 1104 7 0.07868662947233673 1104 8 0.022664677849498105 1104 9 0.09698053296590814 1104 10-11 0.11912469679766247 1104 12-13 0.1644308597604507 1104 14-15 0.17350468218830173 1104 16-17 0.15762782783296103 1104 18-19 0.22045485779666052 1104 20-21 0.19382758729236116 1104 22-23 0.22065157930362744 1104 24-25 0.19077494873791778 1104 26-27 0.23212491040592198 1104 28-29 0.2437727407713055 1104 30-31 0.27689292295040246 1104 32-33 0.2681441398332751 1104 34-35 0.23845160988211944 1104 36-37 0.26682416332347714 1104 38-39 0.24883932929905228 1104 40-41 0.23921552672270963 1104 42-43 0.2955212807213954 1104 44-45 0.1796951148334216 1104 46-47 0.14431619419151076 1104 48-49 0.20041951289069715 1104 50-51 0.1489517654471939 1104 52-53 0.14206433336257618 1104 54-55 0.10008380499803593 1104 56-57 0.14112370509216987 1104 58-59 0.1274968329534829 1104 60-61 0.05635138196031164 1104 62-63 0.16098538191391043 1104 64-65 0.18165438003948253 1104 66-67 0.25655736815133423 1104 68-69 0.14534116335543246 1104 70-71 0.06041215996024363 1104 72-73 0.19466893210893943 1104 74-75 0.21854629137952486 1104 76-77 0.25725741721954876 1104 78-79 0.18503018988771913 1104 80-81 0.23400840015143842 1104 82-83 0.21975907370829617 1104 84-85 0.1818232606737311 1104 86-87 0.08000506258753859 1104 88-89 0.10114927713834732 1104 90-91 0.09339081696923301 1104 92-93 0.12018024054927423 1104 94-95 0.07879465503734195 1104 96-97 0.17272949434495644 1104 98-99 0.23280647369092122 1104 100 0.23847554165220686 1105 1 0.06812297364361797 1105 2 0.12219335796574171 1105 3 0.09430815313324103 1105 4 0.0715445731770572 1105 5 0.10492120563340279 1105 6 0.052403610485093566 1105 7 0.06250758260274836 1105 8 0.05225273684931864 1105 9 0.1979494702519844 1105 10-11 0.1306809155150077 1105 12-13 0.16184648761086606 1105 14-15 0.2246755914154761 1105 16-17 0.16533474155010452 1105 18-19 0.1874135172105582 1105 20-21 0.13518908172290622 1105 22-23 0.10799846089603449 1105 24-25 0.1934938836819171 1105 26-27 0.0840257802096005 1105 28-29 0.13512307841320137 1105 30-31 0.18159521128440304 1105 32-33 0.20575107680082283 1105 34-35 0.14810163590043146 1105 36-37 0.09858532628668826 1105 38-39 0.12185718952919444 1105 40-41 0.07041161111572336 1105 42-43 0.07567101163839851 1105 44-45 0.08484622970320999 1105 46-47 0.05282371395222896 1105 48-49 0.1641410002694741 1105 50-51 0.2693820597905372 1105 52-53 0.23299982583259293 1105 54-55 0.2551348494352723 1105 56-57 0.1838403660952821 1105 58-59 0.2924752901314278 1105 60-61 0.42143046315192834 1105 62-63 0.37148907259663133 1105 64-65 0.2787878518532594 1105 66-67 0.30299813965604727 1105 68-69 0.2534283021331092 1105 70-71 0.22581431960792742 1105 72-73 0.23344750221743027 1105 74-75 0.1897734870312746 1105 76-77 0.2037467761130074 1105 78-79 0.2312774209175572 1105 80-81 0.24080680209051053 1105 82-83 0.29357507015856044 1105 84-85 0.354995995866922 1105 86-87 0.3714989480711921 1105 88-89 0.3428687682184517 1105 90-91 0.316602388006892 1105 92-93 0.35565448497492724 1105 94-95 0.24033643806249927 1105 96-97 0.24811531909139717 1105 98-99 0.2469227921550683 1105 100 0.1764919157299616 1106 1 0.07015959881085365 1106 2 0.13922407290273497 1106 3 0.07578618545872473 1106 4 0.0358372872205166 1106 5 0.08975465806406646 1106 6 0.12088842872065442 1106 7 0.023636000361278775 1106 8 0.05099886720063296 1106 9 0.04639112128585765 1106 10-11 0.07724557643658869 1106 12-13 0.06696020628390897 1106 14-15 0.04045718436556456 1106 16-17 0.060915804628670855 1106 18-19 0.046306719573358635 1106 20-21 0.042301890630326966 1106 22-23 0.1567821058910468 1106 24-25 0.0891333795208098 1106 26-27 0.11545712719428991 1106 28-29 0.08156356971909418 1106 30-31 0.11491450452393082 1106 32-33 0.14219104464657306 1106 34-35 0.13540691582784348 1106 36-37 0.18963492880189392 1106 38-39 0.18018546071887442 1106 40-41 0.185835993240012 1106 42-43 0.1514615480470738 1106 44-45 0.1992669074339588 1106 46-47 0.23386930327298927 1106 48-49 0.21025317250328612 1106 50-51 0.11492195617789491 1106 52-53 0.1570607728247495 1106 54-55 0.13448641622453295 1106 56-57 0.12135403591204152 1106 58-59 0.1148147430790516 1106 60-61 0.1130607006445814 1106 62-63 0.12429881593478598 1106 64-65 0.12952785812666434 1106 66-67 0.0854270028426356 1106 68-69 0.0362931792153347 1106 70-71 0.09378630921501241 1106 72-73 -0.004342181235266196 1106 74-75 0.03979302548550834 1106 76-77 -0.018433041779672976 1106 78-79 -0.008059513334629287 1106 80-81 0.03929412400754018 1106 82-83 0.005914040224354267 1106 84-85 0.012296377992832674 1106 86-87 0.0810998969542922 1106 88-89 0.11770887954909881 1106 90-91 0.18144031216684198 1106 92-93 0.16307045440485268 1106 94-95 0.13120899742957448 1106 96-97 0.10554202148320257 1106 98-99 0.14065429605606283 1106 100 0.1581748684996498 1107 1 0.08273237355076901 1107 2 0.05590412476747275 1107 3 0.08022890728637577 1107 4 0.05971298877948783 1107 5 0.10546694070978901 1107 6 0.04667778682863144 1107 7 0.12446689138093348 1107 8 0.0685125259223156 1107 9 0.14305475397429745 1107 10-11 0.12729244402866158 1107 12-13 0.14834453838580686 1107 14-15 0.16829688323352343 1107 16-17 0.10370343313627473 1107 18-19 0.13780715759911288 1107 20-21 0.15993353308336822 1107 22-23 0.17007962121030573 1107 24-25 0.09762289289709258 1107 26-27 0.12202867747956958 1107 28-29 0.18606840662530288 1107 30-31 0.2137230667053629 1107 32-33 0.13772116444604166 1107 34-35 0.23976994722990952 1107 36-37 0.08175003840145223 1107 38-39 0.13821665715383347 1107 40-41 0.08879641107545666 1107 42-43 0.18387138030920624 1107 44-45 0.1755153584367548 1107 46-47 0.19450828547028465 1107 48-49 0.1811900277344094 1107 50-51 0.16041902035777866 1107 52-53 0.11137980766273614 1107 54-55 0.07643597614249131 1107 56-57 0.18995511588413194 1107 58-59 0.13165623207176935 1107 60-61 0.16344898778152128 1107 62-63 0.01661644940072904 1107 64-65 0.13681503153753027 1107 66-67 0.10494165168099556 1107 68-69 0.02845740667213903 1107 70-71 0.08197377492756885 1107 72-73 0.08061890857592147 1107 74-75 0.07052078452400679 1107 76-77 0.06541571867339258 1107 78-79 -0.06473651570205874 1107 80-81 0.021458056602007503 1107 82-83 0.054715696419528115 1107 84-85 0.03220947895912474 1107 86-87 -0.04906863238877435 1107 88-89 -0.03409633749674157 1107 90-91 -0.10983304306389385 1107 92-93 -0.11708872469840514 1107 94-95 -0.043375886840841815 1107 96-97 0.01644218963232902 1107 98-99 0.15880695249411048 1107 100 0.006156652215025815 1108 1 0.04930719636579539 1108 2 0.10640941940822302 1108 3 0.08145694475130227 1108 4 0.0018548150219643844 1108 5 0.027919885122386745 1108 6 0.023798155984138702 1108 7 0.09558134784871442 1108 8 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60-61 0.0036409495333060704 2214 62-63 0.08656745862579385 2214 64-65 0.12830530373214089 2214 66-67 0.07768770197993291 2214 68-69 -0.039399929830246094 2214 70-71 0.06249397075340113 2214 72-73 0.07051945140030114 2214 74-75 0.04570625009079521 2214 76-77 -0.03457302539064955 2214 78-79 0.07609806390588858 2214 80-81 8.016982679137641E-4 2214 82-83 0.053418861602445133 2214 84-85 -0.028544606979949094 2214 86-87 0.05813626476697209 2214 88-89 0.06645657604050825 2214 90-91 0.08251082715241864 2214 92-93 0.030801919765700347 2214 94-95 0.15114247234568268 2214 96-97 0.08258780026341839 2214 98-99 0.07554974543499071 2214 100 0.11380355075854709 2215 1 -0.12117187939136187 2215 2 -0.15783114067157555 2215 3 -0.15382845321232708 2215 4 -0.07524571584325912 2215 5 -0.0376029044060715 2215 6 -0.052660024868274036 2215 7 -0.05494473063534144 2215 8 -0.03515229787792862 2215 9 -0.16020302661868868 2215 10-11 -0.13238892525457402 2215 12-13 -0.17376179575861528 2215 14-15 -0.195608251168629 2215 16-17 -0.22773510279278497 2215 18-19 -0.18258647921612692 2215 20-21 -0.17990159289367114 2215 22-23 -0.19458722448157317 2215 24-25 -0.25439380564562697 2215 26-27 -0.2520790150699028 2215 28-29 -0.26567387769543416 2215 30-31 -0.2938327198063888 2215 32-33 -0.2272986069073326 2215 34-35 -0.12727684053160715 2215 36-37 -0.22404057316098402 2215 38-39 -0.1901427475804951 2215 40-41 -0.12754531116077317 2215 42-43 -0.12491551203856233 2215 44-45 -0.19095711370329838 2215 46-47 -0.22317223105162753 2215 48-49 -0.2948484380878327 2215 50-51 -0.3009834508734812 2215 52-53 -0.1998645815708855 2215 54-55 -0.22799964686866758 2215 56-57 -0.22325062440221188 2215 58-59 -0.17078175653211503 2215 60-61 -0.15081764772655504 2215 62-63 -0.21902224355120836 2215 64-65 -0.2406926026579228 2215 66-67 -0.3241775169313783 2215 68-69 -0.32894747251658885 2215 70-71 -0.3737723194870526 2215 72-73 -0.23683789475804673 2215 74-75 -0.3645412354133839 2215 76-77 -0.34981755049785335 2215 78-79 -0.4050950512758078 2215 80-81 -0.38015289700791044 2215 82-83 -0.4733984369378632 2215 84-85 -0.48652308401914723 2215 86-87 -0.5404111916703798 2215 88-89 -0.5108948063528445 2215 90-91 -0.5814683085712673 2215 92-93 -0.5801859565722722 2215 94-95 -0.6174769513575669 2215 96-97 -0.6293241373647405 2215 98-99 -0.5950972662828988 2215 100 -0.6224230833606335 2216 1 -0.4024388312364948 2216 2 -0.3912102601313876 2216 3 -0.3173817924352136 2216 4 -0.16135721442511652 2216 5 -0.2667576240580729 2216 6 -0.2705990808862069 2216 7 -0.21988779826706661 2216 8 -0.26689564500522067 2216 9 -0.29816941223990767 2216 10-11 -0.35575386328508074 2216 12-13 -0.3973061004552747 2216 14-15 -0.5547761964887599 2216 16-17 -0.5362473830322969 2216 18-19 -0.5477896042968453 2216 20-21 -0.6023469013978797 2216 22-23 -0.5235723288145309 2216 24-25 -0.6060748474769753 2216 26-27 -0.5384844747910265 2216 28-29 -0.5401403950567101 2216 30-31 -0.6304056314682285 2216 32-33 -0.7069360505303379 2216 34-35 -0.6131265703353392 2216 36-37 -0.5120793835224688 2216 38-39 -0.5693532630199059 2216 40-41 -0.5392970728610962 2216 42-43 -0.5122357901908927 2216 44-45 -0.6125595118888967 2216 46-47 -0.5538094137776639 2216 48-49 -0.5700009401155981 2216 50-51 -0.511068797763123 2216 52-53 -0.5833248438627479 2216 54-55 -0.5340684397164779 2216 56-57 -0.5531532271723663 2216 58-59 -0.6638139023967682 2216 60-61 -0.8342322530488531 2216 62-63 -0.798198338672691 2216 64-65 -0.8183556245164354 2216 66-67 -0.8583372607873798 2216 68-69 -0.6996697954812063 2216 70-71 -0.7219825921114662 2216 72-73 -0.6981838131412061 2216 74-75 -0.7124526712249803 2216 76-77 -0.7170216390891682 2216 78-79 -0.7558913682372079 2216 80-81 -0.6819837285717867 2216 82-83 -0.7591824904501507 2216 84-85 -0.8593716527022153 2216 86-87 -0.7253232099796634 2216 88-89 -0.730991590087342 2216 90-91 -0.6637432009835607 2216 92-93 -0.7923830190273904 2216 94-95 -0.7320719088132748 2216 96-97 -0.6248425538492981 2216 98-99 -0.6901599848809781 2216 100 -0.7608880146749861 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 3.0 4 1.0 5 7.0 6 34.0 7 70.0 8 155.0 9 268.0 10 455.0 11 775.0 12 1244.0 13 2032.0 14 2479.0 15 3085.0 16 3923.0 17 5881.0 18 7127.0 19 3962.0 20 2519.0 21 2280.0 22 2262.0 23 2325.0 24 2542.0 25 3023.0 26 3749.0 27 4873.0 28 5990.0 29 7970.0 30 9803.0 31 12519.0 32 15848.0 33 20845.0 34 29299.0 35 45925.0 36 84714.0 37 191636.0 38 357512.0 39 199101.0 40 985.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28530453855239 20.439634601942682 13.481645737424378 22.793415122080553 2 15.638072742172623 20.66523656872906 40.52958615536648 23.167104533731834 3 17.735768289649506 26.098844417706456 31.524277254908768 24.641110037735267 4 11.782118741062794 17.337722508116403 39.78750300116575 31.092655749655044 5 11.508829844362968 38.69811737325025 36.47853254031686 13.314520242069916 6 29.37357305936073 39.5086117795878 17.668803221029247 13.449011940022215 7 26.053225018053084 33.74128318127608 22.929198173191967 17.276293627478864 8 25.227224014783317 36.82525522272491 20.141982424533822 17.805538337957948 9 25.060691897270164 15.784906759120174 21.648062197866192 37.50633914574347 10-11 23.668348394324482 26.72428504078664 29.46823597920224 20.139130585686644 12-13 24.526897555731775 24.435065764577995 29.732289841379263 21.30574683831097 14-15 21.526910747427028 26.242088264359225 27.24833088288462 24.982670105329124 16-17 20.424458676837375 29.88627368936597 27.76855982345234 21.92070781034431 18-19 20.376629950107258 28.071103183976476 30.749210634143992 20.803056231772278 20-21 21.496403390964563 27.282070366467515 30.711502882204183 20.51002336036374 22-23 21.165927194266246 27.13391295628918 30.73206803531728 20.968091814127295 24-25 20.96007412106569 27.299266695075886 30.670724300101615 21.069934883756805 26-27 20.699035922237425 27.183733995913826 30.88809119545064 21.229138886398104 28-29 20.827602431878805 27.31992434969033 30.8817850619082 20.97068815652267 30-31 21.01030876143476 27.66462342791629 30.448114367372202 20.87695344327674 32-33 20.733963974388644 27.31186839466173 30.96032939905886 20.993838231890766 34-35 20.98978248605278 27.15997473347188 30.829937942706703 21.020304837768638 36-37 21.231082170040207 27.565984243159008 30.271517848574415 20.931415738226374 38-39 20.95342862668294 27.84767210651224 30.354222539663095 20.844676727141724 40-41 20.898718700034006 27.544500635464974 30.523782306822266 21.032998357678757 42-43 20.870786340693442 27.53063174799354 30.304916082590307 21.293665828722713 44-45 21.06364496264695 27.642655830743397 30.145165337914793 21.148533868694862 46-47 21.207015545591876 28.028349831319954 29.573835528506688 21.190799094581486 48-49 21.21547318620361 27.943451144253828 29.398001688184017 21.44307398135855 50-51 21.246841197921995 27.96676428627965 29.308519161307885 21.477875354490468 52-53 21.29175796067681 28.06125305251778 29.197465324363193 21.449523662442207 54-55 21.45023460628519 28.36781400610623 28.99513605813178 21.1868153294768 56-57 21.179985726562826 28.11852646786474 29.04678242080004 21.654705384772395 58-59 21.130236779669115 27.739811130203 29.387632796814177 21.742319293313706 60-61 21.383019893089003 28.12377857707426 29.085668734178803 21.407532795657936 62-63 21.16288428578046 28.0686889118787 29.240123604527273 21.528303197813567 64-65 21.194155184930587 27.828286382826516 29.354181871470757 21.623376560772137 66-67 21.158445141924158 27.845509153497332 29.15491457266084 21.841131131917667 68-69 21.441314891202644 28.057806081892434 29.039221423671684 21.46165760323324 70-71 21.507394234820342 27.816765278960148 29.09194419350827 21.583896292711238 72-73 21.1960834031509 28.053791503692054 29.098162697268776 21.651962395888265 74-75 21.345857753413668 28.120797086847915 28.928142055692728 21.605203104045692 76-77 21.451630863654177 27.968818768128273 28.83241991985357 21.747130448363976 78-79 21.469552952246712 28.15675835665674 28.85231487417894 21.521373816917606 80-81 21.41009838265642 27.96908676078635 28.942115287370008 21.67869956918722 82-83 21.3239345504574 28.005599548411077 29.025820300580442 21.644645600551087 84-85 21.210062985248165 28.131402287059633 28.89815826236687 21.760376465325333 86-87 21.42698063473638 28.034125902172928 28.857916079358436 21.68097738373225 88-89 21.168322692731163 28.054360590114747 28.814949933572564 21.962366783581526 90-91 21.167614386835655 27.976063075935237 28.85436732206867 22.001955215160436 92-93 21.286308734149877 28.05741523462808 28.692818082250948 21.96345794897109 94-95 21.06945798227372 27.91971207567944 28.91357353379283 22.097256408254008 96-97 21.0880195599022 27.9253083229852 28.87601906627691 22.110653050835694 98-99 21.058852622835996 27.991764576011125 28.7206314146428 22.228751386510083 100 21.09682837026032 27.850316275993585 28.751372650345587 22.30148270340051 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 46.0 1 49.5 2 174.0 3 328.0 4 211.5 5 86.0 6 234.0 7 633.5 8 1059.5 9 1263.0 10 984.5 11 616.0 12 602.0 13 680.0 14 814.0 15 928.0 16 1018.0 17 1175.0 18 1386.5 19 1580.0 20 1837.0 21 2326.5 22 3080.5 23 4350.5 24 5933.0 25 7574.0 26 9714.0 27 12349.5 28 15297.5 29 18025.5 30 20355.0 31 22997.5 32 25445.0 33 27483.0 34 29631.0 35 31218.5 36 32484.0 37 33850.5 38 34613.0 39 34606.0 40 34773.0 41 35299.0 42 35320.0 43 35284.5 44 34685.5 45 33880.5 46 33786.5 47 33314.0 48 32869.0 49 31572.0 50 29878.0 51 28802.5 52 26935.5 53 25240.0 54 24242.5 55 23047.5 56 21230.5 57 18982.0 58 17037.0 59 15743.0 60 14320.0 61 12483.0 62 10677.0 63 9032.0 64 7493.0 65 6024.5 66 4880.5 67 3767.5 68 2675.5 69 1905.0 70 1356.5 71 992.5 72 762.0 73 559.5 74 433.0 75 318.5 76 198.0 77 150.5 78 100.5 79 62.5 80 43.5 81 22.0 82 13.5 83 5.5 84 3.0 85 2.0 86 0.5 87 2.0 88 2.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.928218220312429E-4 2 1.928218220312429E-4 3 9.641091101562145E-5 4 0.01253341843203079 5 5.784654660937287E-4 6 0.004145669173671723 7 7.712872881249716E-4 8 0.14124198463788545 9 0.002024629131328051 10-11 0.0 12-13 4.338490995702966E-4 14-15 1.928218220312429E-4 16-17 0.0 18-19 1.928218220312429E-4 20-21 0.0 22-23 9.641091101562145E-5 24-25 4.820545550781073E-4 26-27 0.006748763771093501 28-29 0.008339543802851256 30-31 0.014268814830311977 32-33 0.01802884035992121 34-35 0.027187876906405253 36-37 0.03928744623886574 38-39 0.044686457255740546 40-41 0.05635217748863074 42-43 0.07341690873839574 44-45 0.11251153315523024 46-47 0.11974235148140185 48-49 0.1159341204962848 50-51 0.11887465328226125 52-53 0.1154038604856989 54-55 0.11916388601530813 56-57 0.09992990926769164 58-59 0.09742322558128548 60-61 0.10007452563421508 62-63 0.09824271832491827 64-65 0.0 66-67 4.820545550781073E-5 68-69 4.820545550781073E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 4.820545550781073E-5 78-79 0.0 80-81 1.4461636652343218E-4 82-83 4.820545550781073E-5 84-85 0.0 86-87 8.676981991405932E-4 88-89 4.820545550781073E-4 90-91 9.641091101562145E-5 92-93 0.001060520021171836 94-95 9.641091101562145E-4 96-97 2.8923273304686436E-4 98-99 4.820545550781073E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1037227.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.408671535874 #Duplication Level Percentage of deduplicated Percentage of total 1 83.98606985653217 61.65305815687139 2 10.743144922379122 15.772799937384432 3 2.533876119934239 5.580254394025422 4 1.0072498400156331 2.9576349064107705 5 0.49682961247792506 1.8235800915843785 6 0.30521119274918634 1.344308891855841 7 0.19103669630442094 0.9816625063216826 8 0.14317790982191567 0.8408400122647998 9 0.09468048333589117 0.6255331651856005 >10 0.47318614288580824 5.9109826088571 >50 0.01882999054096573 0.9039347262344524 >100 0.006181176599573197 0.7807423647232268 >500 1.3151410577949665E-4 0.06690413124896373 >1k 3.9454231733848996E-4 0.7577641070319139 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3273 0.31555291175412903 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2848 0.27457827457248996 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1728 0.16659805423499388 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 4.820545550781073E-5 0.0 26-27 0.0 0.0 0.0 2.4102727753905364E-4 0.0 28-29 0.0 0.0 0.0 0.0013497527542187004 0.0 30-31 0.0 0.0 0.0 0.003711820074101426 0.0 32-33 0.0 0.0 0.0 0.0077610783367575275 0.0 34-35 0.0 0.0 0.0 0.014606253018866651 0.0 36-37 0.0 0.0 0.0 0.02641658961828028 0.0 38-39 0.0 0.0 0.0 0.05027829009464659 0.0 40-41 0.0 0.0 0.0 0.07963541249890332 0.0 42-43 0.0 0.0 0.0 0.10908894581417568 0.0 44-45 0.0 0.0 0.0 0.14341123013573692 0.0 46-47 0.0 0.0 0.0 0.18607305826014942 0.0 48-49 0.0 0.0 0.0 0.24136471572760831 0.0 50-51 0.0 0.0 0.0 0.30562258791952 0.0 52-53 0.0 0.0 0.0 0.36549376366022096 0.0 54-55 0.0 0.0 0.0 0.4267146921551406 0.0 56-57 0.0 0.0 0.0 0.5007582718151379 0.0 58-59 0.0 0.0 0.0 0.5870942426296268 0.0 60-61 0.0 0.0 0.0 0.6906877665159121 0.0 62-63 0.0 0.0 0.0 0.7937992358471193 0.0 64-65 0.0 0.0 0.0 0.899320977953717 0.0 66-67 0.0 0.0 0.0 1.0275474896044936 0.0 68-69 0.0 0.0 0.0 1.1716335961173399 0.0 70-71 0.0 0.0 0.0 1.3324469956913965 0.0 72-73 0.0 0.0 0.0 1.4986594062823277 0.0 74-75 0.0 0.0 0.0 1.6582194640131813 0.0 76-77 0.0 0.0 0.0 1.828143694678214 0.0 78-79 0.0 0.0 0.0 2.0386569188808235 0.0 80-81 0.0 0.0 0.0 2.2741887744919866 0.0 82-83 0.0 0.0 0.0 2.5212899394250243 0.0 84-85 0.0 0.0 0.0 2.752724331318024 0.0 86-87 0.0 0.0 0.0 2.9855084759652417 0.0 88 0.0 0.0 0.0 3.18262058353668 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2395 0.0 50.41644 1 GTATCAA 3645 0.0 33.061718 2 GTACATG 3075 0.0 27.044111 1 TCAACGC 4555 0.0 26.348194 5 ATCAACG 4600 0.0 25.993147 4 CAACGCA 4625 0.0 25.847649 6 TACCTGG 770 0.0 25.676838 2 TATCAAC 4880 0.0 25.080517 3 AACGCAG 4755 0.0 25.042004 7 GTACCTG 800 0.0 24.666292 1 TACATGG 3170 0.0 24.650845 2 ACATGGG 3080 0.0 23.68994 3 ACGCAGA 5370 0.0 21.91113 7 CGCAGAG 5460 0.0 21.54996 8 ACCTGGG 910 0.0 21.209257 3 GAGTACT 4045 0.0 20.903296 12-13 CTGTCGC 205 7.598301E-7 20.622887 9 AGAGTAC 5155 0.0 20.50287 10-11 GTATAGA 460 0.0 20.42757 1 CAGAGTA 5780 0.0 19.911274 10-11 >>END_MODULE