FastQCFastQC Report
Wed 25 May 2016
SRR1032920_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032920_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5114437
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT307740.6017084578419873No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT162060.316867721706221No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT129210.2526377781171222No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC109370.2138456295385005No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT91300.17851427244093535No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT74800.1462526569395615No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC72440.14163826829815288No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC62950.12308295126130207No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA57990.11338491411664667No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC53260.10413658433958617No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG53090.10380419193745079No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG219550.028.4729981
TACATGG223100.027.0297532
ACATGGG227000.025.6957263
CATGGGG156800.022.5716824
GAGTACA154400.018.5389421
AGTACAT152450.017.635332
GTATTAG26700.017.4276541
TATACTG18850.016.9549125
CATGGGA69650.016.7357644
AGAGTAC136900.015.79209110-11
ATGGGGG88500.015.7728875
GTATTAA20650.015.705221
GTACTAG5157.8216544E-1114.6025181
ATGGGGC55250.014.5466115
ATGGGGA70600.014.5127535
CCCTATA18850.014.4621082
TAGTGCG2601.3327076E-414.4615425
ACCTATC10150.013.8913588
CATGGGC64050.013.5024844
GTATATA19250.013.4291021
Produced by FastQC (version 0.11.5)