##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032920_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5114437 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.836422464486315 33.0 31.0 34.0 30.0 34.0 2 31.90012722808004 34.0 31.0 34.0 30.0 34.0 3 32.0260055994433 34.0 31.0 34.0 30.0 34.0 4 35.31647804049595 37.0 35.0 37.0 33.0 37.0 5 35.41580725307595 37.0 35.0 37.0 35.0 37.0 6 35.466700440341725 37.0 37.0 37.0 35.0 37.0 7 35.42706831661041 37.0 37.0 37.0 35.0 37.0 8 35.40049276195992 37.0 37.0 37.0 35.0 37.0 9 37.07848820896611 39.0 38.0 39.0 35.0 39.0 10-11 37.072004015300216 39.0 38.0 39.0 35.0 39.0 12-13 37.02377192250095 39.0 38.0 39.0 35.0 39.0 14-15 38.50718583492181 41.0 39.0 41.0 35.0 41.0 16-17 38.5341265324023 41.0 39.0 41.0 35.0 41.0 18-19 38.55787812030923 41.0 39.5 41.0 35.0 41.0 20-21 38.5954341602018 41.0 39.0 41.0 35.0 41.0 22-23 38.59178175427716 41.0 39.0 41.0 35.0 41.0 24-25 38.621706455666576 41.0 39.0 41.0 35.0 41.0 26-27 38.594499062164616 41.0 39.0 41.0 34.5 41.0 28-29 38.57491626155527 41.0 39.0 41.0 34.0 41.0 30-31 38.5242815582634 41.0 39.0 41.0 34.0 41.0 32-33 38.46374869804829 41.0 39.0 41.0 34.0 41.0 34-35 38.36779053100077 41.0 39.0 41.0 34.0 41.0 36-37 38.27102142425451 41.0 38.5 41.0 33.5 41.0 38-39 38.171308493975 41.0 38.0 41.0 33.0 41.0 40-41 38.089495969937644 40.5 38.0 41.0 33.0 41.0 42-43 37.95913098548286 40.0 38.0 41.0 33.0 41.0 44-45 37.80743462085856 40.0 38.0 41.0 33.0 41.0 46-47 37.659463201912544 40.0 38.0 41.0 33.0 41.0 48-49 37.488950689978196 40.0 37.0 41.0 32.0 41.0 50-51 37.321716935803494 40.0 37.0 41.0 32.0 41.0 52-53 37.157830568643234 40.0 36.5 41.0 31.5 41.0 54-55 36.94263366622758 40.0 36.0 41.0 31.0 41.0 56-57 36.702933186976395 39.5 35.0 41.0 31.0 41.0 58-59 36.450985025331235 39.0 35.0 41.0 31.0 41.0 60-61 36.14491947011959 39.0 35.0 41.0 30.0 41.0 62-63 35.879093241347974 38.0 35.0 40.0 30.0 41.0 64-65 35.57948675484711 37.5 35.0 40.0 30.0 41.0 66-67 35.21899370351028 37.0 35.0 40.0 30.0 41.0 68-69 34.85453941460223 36.0 34.0 39.0 29.0 41.0 70-71 34.479748504087546 36.0 34.0 39.0 29.0 41.0 72-73 34.181073009600084 35.0 34.0 38.5 29.0 40.0 74-75 33.91757704709238 35.0 34.0 37.0 29.0 40.0 76-77 33.60891550722005 35.0 34.0 37.0 29.0 39.0 78-79 33.262387727134 35.0 34.0 36.5 28.5 39.0 80-81 32.9571500245286 35.0 34.0 36.0 28.0 38.0 82-83 32.67834885833964 35.0 33.5 36.0 27.5 37.0 84-85 32.57360643996592 35.0 34.0 35.0 27.5 37.0 86-87 32.41661770396233 35.0 34.0 35.0 27.0 36.5 88-89 32.21748503305447 35.0 33.0 35.0 27.0 36.0 90-91 32.03976752475394 35.0 33.0 35.0 27.0 36.0 92-93 31.85853467351343 35.0 33.0 35.0 26.0 36.0 94-95 31.684666660279518 35.0 33.0 35.0 25.0 35.0 96-97 31.527364302268264 35.0 33.0 35.0 25.0 35.0 98-99 31.369402536388655 35.0 33.0 35.0 25.0 35.0 100 31.231283326004405 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38881970893924134 1101 2 0.44292922846060634 1101 3 0.43380868103275816 1101 4 0.3805207379680198 1101 5 0.30709303957516454 1101 6 0.3429863204628987 1101 7 0.30113093389351064 1101 8 0.3250389863317551 1101 9 0.3775379775154306 1101 10-11 0.40176213614488887 1101 12-13 0.21262395077845753 1101 14-15 0.29646748622013774 1101 16-17 0.3185283460659676 1101 18-19 0.37933853213939983 1101 20-21 0.3110158747030951 1101 22-23 0.3080096689238374 1101 24-25 0.32098435558406635 1101 26-27 0.3725802599561874 1101 28-29 0.42019558752620156 1101 30-31 0.40130556257732763 1101 32-33 0.39837567777863825 1101 34-35 0.453665264090354 1101 36-37 0.42076726594716973 1101 38-39 0.4185147695439184 1101 40-41 0.42998896163150135 1101 42-43 0.45035151743626756 1101 44-45 0.45275812410412186 1101 46-47 0.4507355590120383 1101 48-49 0.4683779448708236 1101 50-51 0.42881718009735437 1101 52-53 0.41889474198082155 1101 54-55 0.4816281228294059 1101 56-57 0.4559783280352434 1101 58-59 0.39448239500757865 1101 60-61 0.4443145575450771 1101 62-63 0.4631630799356117 1101 64-65 0.47692921182425607 1101 66-67 0.464382845405261 1101 68-69 0.4605214772302091 1101 70-71 0.4474573114356062 1101 72-73 0.3967745630922508 1101 74-75 0.3502142122897425 1101 76-77 0.3737249325664749 1101 78-79 0.40329615686069786 1101 80-81 0.4570737481519913 1101 82-83 0.3924840289875391 1101 84-85 0.35833996044657823 1101 86-87 0.286818012066675 1101 88-89 0.3054475495142839 1101 90-91 0.3067954207238799 1101 92-93 0.33158776850958915 1101 94-95 0.3505114012084434 1101 96-97 0.29065685652632567 1101 98-99 0.29471022864939656 1101 100 0.31024636542261774 1102 1 0.24871312053258166 1102 2 0.23598516027585958 1102 3 0.24257797184892382 1102 4 0.261047553312423 1102 5 0.21515377369541255 1102 6 0.20850595157109808 1102 7 0.21234942286569947 1102 8 0.21467457799215595 1102 9 0.25424152944221134 1102 10-11 0.2234367076573207 1102 12-13 0.2664618525201732 1102 14-15 0.28652951894516576 1102 16-17 0.2768663716530426 1102 18-19 0.2532469330148075 1102 20-21 0.24379150070250688 1102 22-23 0.2416804224115907 1102 24-25 0.27415555025673655 1102 26-27 0.29822748569225865 1102 28-29 0.28614119612696953 1102 30-31 0.27213595806986746 1102 32-33 0.33155293431548927 1102 34-35 0.34161237933798105 1102 36-37 0.35771276027553967 1102 38-39 0.3571289348285376 1102 40-41 0.3368468770225945 1102 42-43 0.4051374127250682 1102 44-45 0.40399166296193556 1102 46-47 0.39550011943342156 1102 48-49 0.47879606726681345 1102 50-51 0.3823050170972806 1102 52-53 0.3720636753263875 1102 54-55 0.4169751792523826 1102 56-57 0.38041858927580563 1102 58-59 0.3564263893223014 1102 60-61 0.43221170877043136 1102 62-63 0.40849841816154964 1102 64-65 0.43097292071426807 1102 66-67 0.4080987342281617 1102 68-69 0.41805288744053115 1102 70-71 0.4353515983132894 1102 72-73 0.3961666618344566 1102 74-75 0.360937463089158 1102 76-77 0.35416219181993114 1102 78-79 0.3726188991931849 1102 80-81 0.34504664252856543 1102 82-83 0.3932500158540506 1102 84-85 0.38881077779421247 1102 86-87 0.39495484361273725 1102 88-89 0.32538525598286583 1102 90-91 0.3496206783304885 1102 92-93 0.3275506400814372 1102 94-95 0.40771127448368105 1102 96-97 0.3434811560840245 1102 98-99 0.39576859757224625 1102 100 0.39147692837396875 1103 1 0.23255019268384203 1103 2 0.2966065461440479 1103 3 0.278960435351685 1103 4 0.2477197989569362 1103 5 0.2402422288514714 1103 6 0.18988251830613478 1103 7 0.2211349478971698 1103 8 0.2122975424715534 1103 9 0.206021520415959 1103 10-11 0.2624296348122357 1103 12-13 0.26279334514756414 1103 14-15 0.31238827783481327 1103 16-17 0.3871710378640927 1103 18-19 0.37049201366026807 1103 20-21 0.3416833507281112 1103 22-23 0.29159527438157085 1103 24-25 0.326758893668341 1103 26-27 0.38878154742911164 1103 28-29 0.3796453071194392 1103 30-31 0.4069775884127509 1103 32-33 0.3818069441648717 1103 34-35 0.3937697521614254 1103 36-37 0.3621037754929759 1103 38-39 0.39612521097186004 1103 40-41 0.4598527182407892 1103 42-43 0.4298124316231906 1103 44-45 0.4139129136838804 1103 46-47 0.44952017594250293 1103 48-49 0.41948723003631017 1103 50-51 0.460121137758712 1103 52-53 0.44575008377003655 1103 54-55 0.4895555387750221 1103 56-57 0.4417514986708895 1103 58-59 0.43865587605852596 1103 60-61 0.43193306929550346 1103 62-63 0.467904858382731 1103 64-65 0.45406815444125925 1103 66-67 0.447242411363554 1103 68-69 0.4458451046972698 1103 70-71 0.4196596401590398 1103 72-73 0.41882647246663396 1103 74-75 0.4373287074938972 1103 76-77 0.41760970527248986 1103 78-79 0.44019818157922685 1103 80-81 0.4360443815245745 1103 82-83 0.44396331034057823 1103 84-85 0.4444866906538252 1103 86-87 0.42986976765877216 1103 88-89 0.40735114639042536 1103 90-91 0.42023261531912937 1103 92-93 0.4603700547641729 1103 94-95 0.4406448714697646 1103 96-97 0.4457167987705546 1103 98-99 0.4432988241946525 1103 100 0.45370568306501013 1104 1 0.002635790677466332 1104 2 0.030925275079507486 1104 3 0.03612329229589051 1104 4 -0.004365548640649308 1104 5 0.008241888029772326 1104 6 0.03221965406586236 1104 7 0.018719388111279045 1104 8 -0.019950917108431554 1104 9 0.05353667253838523 1104 10-11 0.024846008814442655 1104 12-13 0.04852650603567099 1104 14-15 0.07753431728818327 1104 16-17 0.05723734376590528 1104 18-19 0.08364516496381924 1104 20-21 0.008934645613209113 1104 22-23 0.04757270909812661 1104 24-25 0.0375455974248311 1104 26-27 0.0626489279528073 1104 28-29 0.07355172833444357 1104 30-31 0.08580364789640527 1104 32-33 0.10388165001827332 1104 34-35 0.11775863712774282 1104 36-37 0.09770573596750864 1104 38-39 0.09429491645975929 1104 40-41 0.10824356281275982 1104 42-43 0.120689435612654 1104 44-45 0.0782927734827652 1104 46-47 0.09009781967511543 1104 48-49 0.11321011098799261 1104 50-51 0.08461098472826478 1104 52-53 0.11099409369639801 1104 54-55 0.03925026283723554 1104 56-57 0.10526416904517788 1104 58-59 0.10455195098057146 1104 60-61 0.1449614254822862 1104 62-63 0.10271028126658877 1104 64-65 0.08769624821144362 1104 66-67 0.11364340375450155 1104 68-69 0.1497536863532929 1104 70-71 0.10112845170645102 1104 72-73 0.15136186972388543 1104 74-75 0.11284301919373263 1104 76-77 0.12287852567327917 1104 78-79 0.10111782349152065 1104 80-81 0.1448252893151576 1104 82-83 0.11701908466616118 1104 84-85 0.09824187714431076 1104 86-87 0.08809847545105498 1104 88-89 0.09333861323997183 1104 90-91 0.02372059503911572 1104 92-93 -0.03466107105437288 1104 94-95 -0.011373622101476855 1104 96-97 -0.0455732851135231 1104 98-99 -0.022889765996627176 1104 100 -0.008097735652018656 1105 1 -0.045038858207625765 1105 2 -0.02729776668121886 1105 3 -0.019004546859854088 1105 4 -0.050298679141640434 1105 5 -0.027760940222044894 1105 6 -0.04715708260797413 1105 7 -0.08224064257471042 1105 8 -0.07346285382625695 1105 9 -0.05719266848512916 1105 10-11 -0.011654804182036571 1105 12-13 0.03143867241500686 1105 14-15 0.0675804705001255 1105 16-17 0.04490271816472102 1105 18-19 0.07762631543923249 1105 20-21 0.03976510375494513 1105 22-23 0.07924445772871991 1105 24-25 0.050386506671316056 1105 26-27 0.05467327033549907 1105 28-29 0.06665839271844476 1105 30-31 0.09546475983787417 1105 32-33 0.10020310737689186 1105 34-35 0.06855010294396635 1105 36-37 0.061947252027579225 1105 38-39 0.07392917765469065 1105 40-41 0.07025614796881996 1105 42-43 0.08802021321137232 1105 44-45 0.07597923097668513 1105 46-47 0.010672616407397584 1105 48-49 -0.02814366923646361 1105 50-51 0.0151590571412612 1105 52-53 0.024678309844865964 1105 54-55 0.056859625741452646 1105 56-57 0.057687273096668434 1105 58-59 0.07618057034284931 1105 60-61 0.10255597848279052 1105 62-63 0.1097970776091799 1105 64-65 0.1196909018264023 1105 66-67 0.14051272563104789 1105 68-69 0.1412590854642417 1105 70-71 0.10365810366999995 1105 72-73 0.09647207887088172 1105 74-75 0.10992142587173959 1105 76-77 0.050123644081601526 1105 78-79 0.044757276451647954 1105 80-81 0.03296731470630476 1105 82-83 0.044259952211938014 1105 84-85 0.031141301922325226 1105 86-87 0.01832178365550874 1105 88-89 0.05198896894420102 1105 90-91 0.08986508958138728 1105 92-93 0.06832395054550133 1105 94-95 0.08684101900833952 1105 96-97 0.09073801716264285 1105 98-99 0.10582898870583435 1105 100 0.056449930402180826 1106 1 0.07207959777566941 1106 2 0.07270967228792102 1106 3 0.11225150308587217 1106 4 0.10548891651116321 1106 5 0.10209629601288128 1106 6 0.06955672451862682 1106 7 0.12075233462202561 1106 8 0.0860782321687239 1106 9 0.11940211381332233 1106 10-11 0.12248862991644671 1106 12-13 0.16736012258503052 1106 14-15 0.16166874882292603 1106 16-17 0.1456362910718525 1106 18-19 0.12059471495609131 1106 20-21 0.16742388666754948 1106 22-23 0.15977965966764884 1106 24-25 0.15601339117849022 1106 26-27 0.20250942259530547 1106 28-29 0.1927772082243706 1106 30-31 0.1899238712680429 1106 32-33 0.23474367204918423 1106 34-35 0.22180806904388106 1106 36-37 0.20646884121479303 1106 38-39 0.19270079664239148 1106 40-41 0.23776596114561244 1106 42-43 0.18119713519595138 1106 44-45 0.18514117905333904 1106 46-47 0.1971195053237622 1106 48-49 0.15002085617366134 1106 50-51 0.13556771200346418 1106 52-53 0.1821644508404603 1106 54-55 0.1991591905501906 1106 56-57 0.18404921830481413 1106 58-59 0.15373267460447693 1106 60-61 0.1476199960833 1106 62-63 0.1265469609270795 1106 64-65 0.14613524741506723 1106 66-67 0.16608543785308427 1106 68-69 0.07396322444255787 1106 70-71 0.07244967832858151 1106 72-73 0.03387158380104438 1106 74-75 0.04305076918790007 1106 76-77 0.0780973571242427 1106 78-79 0.12945284828877845 1106 80-81 0.06913627005395284 1106 82-83 0.09287415256621046 1106 84-85 0.10127390196034725 1106 86-87 0.11378065321178354 1106 88-89 0.08410452055698414 1106 90-91 0.03451174829618964 1106 92-93 0.020693571209960737 1106 94-95 0.06533112902940275 1106 96-97 0.08880200974627783 1106 98-99 0.04901661309004268 1106 100 0.09621819345972682 1107 1 0.02673827007037488 1107 2 0.09000623151023035 1107 3 0.08997173204468112 1107 4 0.0731792145413479 1107 5 0.07004909724081898 1107 6 0.0547444643497812 1107 7 0.04061124994563414 1107 8 0.05068273459900752 1107 9 0.030135357609694324 1107 10-11 0.025960361817190858 1107 12-13 0.04444951565231037 1107 14-15 0.0854388786515301 1107 16-17 0.12103110112558113 1107 18-19 0.09390241833291668 1107 20-21 0.12264971168673355 1107 22-23 0.13292736368402558 1107 24-25 0.10886680637419488 1107 26-27 0.11290591146713069 1107 28-29 0.08098095278819528 1107 30-31 0.04312168400787897 1107 32-33 0.03962936851886667 1107 34-35 0.05736054410144931 1107 36-37 0.13286977495743457 1107 38-39 0.05649839513306176 1107 40-41 0.02751062090513301 1107 42-43 0.07547948042491015 1107 44-45 0.08920468634420331 1107 46-47 0.0453401819674113 1107 48-49 0.08635564448268696 1107 50-51 0.052825306656828275 1107 52-53 0.02264860599522933 1107 54-55 0.019603102036505504 1107 56-57 0.08251925539870086 1107 58-59 0.02060314978994171 1107 60-61 0.10556152524677742 1107 62-63 0.06341834747095731 1107 64-65 0.07980965263379147 1107 66-67 0.04826631851310026 1107 68-69 0.10535107939267618 1107 70-71 0.08929973588806206 1107 72-73 0.08042441061096639 1107 74-75 0.023638387504604452 1107 76-77 0.04625823586561495 1107 78-79 0.08716124557708582 1107 80-81 0.05909741740621399 1107 82-83 -0.011138510518975409 1107 84-85 0.02856149149730669 1107 86-87 0.02377070821306404 1107 88-89 0.05212461840810079 1107 90-91 0.07436123026088381 1107 92-93 0.09828589021737955 1107 94-95 0.07068670270553667 1107 96-97 0.027930958966024377 1107 98-99 0.08563707228676876 1107 100 0.07207045746586616 1108 1 0.0035641214635617757 1108 2 0.03203177330589568 1108 3 0.06062614380938669 1108 4 -0.0029496554276917664 1108 5 0.005118756683543779 1108 6 -0.06058719199696583 1108 7 -0.02050175315034153 1108 8 -0.027004571902537577 1108 9 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-0.2115296317373705 2214 62-63 -0.17463364303939954 2214 64-65 -0.24522266422768269 2214 66-67 -0.19112169358040632 2214 68-69 -0.18000925216617247 2214 70-71 -0.1896945211423784 2214 72-73 -0.2425445744602044 2214 74-75 -0.21415619645558337 2214 76-77 -0.20381876621747352 2214 78-79 -0.2345852681366054 2214 80-81 -0.2140579610222062 2214 82-83 -0.22125429572902533 2214 84-85 -0.21268644710932705 2214 86-87 -0.16782084658383667 2214 88-89 -0.17883607045892802 2214 90-91 -0.20904313555325515 2214 92-93 -0.18634816544747324 2214 94-95 -0.15993685793559465 2214 96-97 -0.1985407239989101 2214 98-99 -0.22339913590672822 2214 100 -0.18097531269962275 2215 1 -0.2112013295614723 2215 2 -0.29604450479902056 2215 3 -0.28611541705793897 2215 4 -0.2710031945704756 2215 5 -0.24947115126355612 2215 6 -0.2190548338554592 2215 7 -0.2850511982826234 2215 8 -0.3069946699108854 2215 9 -0.316945291262833 2215 10-11 -0.32457438277554473 2215 12-13 -0.326203469851599 2215 14-15 -0.4107663387897986 2215 16-17 -0.40925597319564133 2215 18-19 -0.41406551194780405 2215 20-21 -0.3928665101264244 2215 22-23 -0.3311588650320374 2215 24-25 -0.3418524757242878 2215 26-27 -0.3308436858637478 2215 28-29 -0.331094950341118 2215 30-31 -0.33162486454251194 2215 32-33 -0.34721448214840933 2215 34-35 -0.3384349575092287 2215 36-37 -0.3293236212689763 2215 38-39 -0.3113291761969066 2215 40-41 -0.28351187104148323 2215 42-43 -0.3743167402377736 2215 44-45 -0.359816137401765 2215 46-47 -0.34910465236301746 2215 48-49 -0.31081793467055263 2215 50-51 -0.30336495450286094 2215 52-53 -0.3227660569131885 2215 54-55 -0.3144071372639985 2215 56-57 -0.31466407409968866 2215 58-59 -0.3042087174442898 2215 60-61 -0.33144995082317763 2215 62-63 -0.31156289965283435 2215 64-65 -0.3325236423500968 2215 66-67 -0.36319643459319906 2215 68-69 -0.3735040172479174 2215 70-71 -0.3793320519628338 2215 72-73 -0.33936390047968956 2215 74-75 -0.3174103929831986 2215 76-77 -0.36427553081639275 2215 78-79 -0.39174631309755625 2215 80-81 -0.4123881440295847 2215 82-83 -0.42092060900033346 2215 84-85 -0.38479777564489126 2215 86-87 -0.38823303085735006 2215 88-89 -0.33260711767105633 2215 90-91 -0.3513624811979241 2215 92-93 -0.34374695607806416 2215 94-95 -0.34642164405010334 2215 96-97 -0.3058373624789823 2215 98-99 -0.308571107648806 2215 100 -0.2655183598374009 2216 1 0.05698351947654956 2216 2 -7.78210294996029E-4 2216 3 -0.055611106435126345 2216 4 0.008791150408335113 2216 5 -0.0047668172484947036 2216 6 -0.004602032087269947 2216 7 -0.02641131537502872 2216 8 -0.0646165528119127 2216 9 -0.058953122200286145 2216 10-11 -0.12207764293339807 2216 12-13 -0.08449214111728764 2216 14-15 -0.11820586600405392 2216 16-17 -0.13985325160601292 2216 18-19 -0.1458738583891801 2216 20-21 -0.149653732493789 2216 22-23 -0.11080036790742298 2216 24-25 -0.16079115087045892 2216 26-27 -0.14995364910293318 2216 28-29 -0.17949039519280063 2216 30-31 -0.20917582193036566 2216 32-33 -0.2867511243552414 2216 34-35 -0.22598060791755614 2216 36-37 -0.23538735093330132 2216 38-39 -0.24266030439189024 2216 40-41 -0.22814703942740522 2216 42-43 -0.20352215259172368 2216 44-45 -0.17525671508096963 2216 46-47 -0.1967420626881804 2216 48-49 -0.11848209304699964 2216 50-51 -0.1318348774286875 2216 52-53 -0.16148574673636062 2216 54-55 -0.1929913160157497 2216 56-57 -0.21076904626401216 2216 58-59 -0.2033937326328541 2216 60-61 -0.21356777997888088 2216 62-63 -0.2015751204625289 2216 64-65 -0.19838334024031212 2216 66-67 -0.2035818109405838 2216 68-69 -0.1366943481607663 2216 70-71 -0.17780685556087406 2216 72-73 -0.19386300031539605 2216 74-75 -0.192449498481146 2216 76-77 -0.1708626073647892 2216 78-79 -0.15970128411412787 2216 80-81 -0.16251333081230968 2216 82-83 -0.17491642025768783 2216 84-85 -0.20437719615114958 2216 86-87 -0.1533436029508337 2216 88-89 -0.10379595760140603 2216 90-91 -0.11781127567735439 2216 92-93 -0.11243946607715927 2216 94-95 -0.0715150490759342 2216 96-97 -0.05536610160404365 2216 98-99 -0.06518092058734126 2216 100 -0.12103761854363171 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53035.0 3 8367.0 4 5432.0 5 4911.0 6 4491.0 7 6172.0 8 7774.0 9 7657.0 10 7302.0 11 7442.0 12 7928.0 13 8773.0 14 9359.0 15 9672.0 16 9540.0 17 9491.0 18 9377.0 19 8860.0 20 8957.0 21 9662.0 22 11789.0 23 17525.0 24 28296.0 25 37723.0 26 37984.0 27 31462.0 28 33581.0 29 42607.0 30 52356.0 31 65422.0 32 86091.0 33 118553.0 34 178600.0 35 298619.0 36 595012.0 37 1199824.0 38 1578503.0 39 495771.0 40 517.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.5252619257368 18.36601233010685 13.09683474032848 24.011891003827866 2 16.077883688332964 20.343534521642326 38.167484526798596 25.411097263226118 3 19.352602834674144 24.998018675136496 29.207448529093778 26.441929961095585 4 12.760665459382938 16.407486829009407 37.415789220315716 33.41605849129194 5 13.540042871137285 38.55588374462203 34.189673276847586 13.714400107393098 6 31.395066456290078 36.78939438977058 17.458139122120635 14.35740003181871 7 28.621054236194293 32.27431329801483 21.598748713127847 17.505883752663028 8 26.394695840950373 34.11904770291262 19.815296852172125 19.670959603964892 9 26.294831631821403 17.001701870687963 19.810600597738784 36.892865899751854 10-11 25.39429548505656 25.95810938547367 27.401287070947404 21.246308058522366 12-13 26.231924867562523 23.745366253804054 27.035854393918523 22.9868544847149 14-15 23.22670240805273 25.210591976327564 25.01583286133975 26.54687275427996 16-17 23.10196511758282 28.339483763080707 25.303048655087984 23.255502464248487 18-19 23.162340689589723 28.05839543176038 26.378081216083938 22.40118266256596 20-21 24.384126687509262 26.54273947258869 25.769580537429842 23.303553302472203 22-23 24.00870883319618 26.204389110260195 26.111021479838296 23.675880576705328 24-25 24.10006900588766 26.28666433092222 25.895073284878865 23.718193378311252 26-27 23.742141133790042 26.255266149688776 26.595587098642593 23.40700561787859 28-29 23.318603386596333 27.264655935016048 26.10592490975266 23.310815768634956 30-31 23.540628810554644 26.560992502633603 25.860430148787927 24.037948538023826 32-33 23.765206285372244 26.611314930195253 26.02524347569665 23.598235308735852 34-35 24.20661681696942 26.168175564282247 26.041165770283285 23.58404184846505 36-37 23.88442312574505 26.204589401164885 26.350183117214094 23.560804355875966 38-39 23.479647810565858 26.98714103729703 26.222326404199176 23.310884747937934 40-41 23.515322118760924 26.868530665859446 26.04011738064354 23.576029834736083 42-43 23.389896543265902 26.93670362437591 25.696209058547538 23.97719077381066 44-45 23.61430418280787 26.335419308625358 25.953206302757437 24.097070205809338 46-47 24.081973157669484 26.639241042625112 25.9591515799316 23.319634219773807 48-49 23.850472842443224 26.536330642442813 25.971915858536804 23.64128065657716 50-51 22.843379232955357 26.773664020402965 26.710981113716535 23.67197563292514 52-53 23.721984423766244 26.85510846140659 25.76263700048299 23.66027011434418 54-55 23.206198013202396 26.67104841627835 26.00261143666004 24.120142133859222 56-57 23.601300992476435 26.318884058438986 26.141426179733834 23.938388769350745 58-59 24.193456694680034 25.873084381677774 25.97861272643462 23.954846197207573 60-61 23.59671823721539 26.390997314250676 26.607851342811063 23.40443310572287 62-63 23.164294054237264 26.51758498318374 26.356971200769774 23.96114976180922 64-65 23.22657014719402 27.04314922958847 25.811144382741247 23.919136240476263 66-67 23.463682231289567 26.316128839094375 25.77969717955828 24.440491750057777 68-69 23.3489371374092 26.460194057534704 25.97991795014095 24.21095085491515 70-71 23.88360733129007 26.297571320595953 25.93764049478625 23.881180853327724 72-73 23.537385879652504 25.991984791454882 26.5804157693749 23.89021355951772 74-75 23.176034112192923 26.930480879499857 26.050544648581734 23.842940359725485 76-77 23.532384410648135 26.159254363937766 25.85374520957243 24.454616015841665 78-79 23.468743597485624 26.392937631609275 26.05684170606914 24.08147706483596 80-81 23.62223191302232 26.22045130840967 26.175140240738653 23.98217653782936 82-83 23.462348406937913 25.96788211813137 26.61753487121078 23.95223460371994 84-85 23.177930679824886 26.31302056467741 26.393866837197542 24.11518191830016 86-87 23.512813704009044 26.355324404517884 26.187916452963062 23.94394543851001 88-89 23.103499961940557 26.520337733030917 25.818410085889106 24.557752219139417 90-91 23.399899737525207 25.937837656973194 26.109138951971456 24.553123653530143 92-93 23.855010115268467 26.330506589159647 26.09150292011237 23.722980375459514 94-95 23.522720324112733 25.973553817653322 26.294036816850575 24.20968904138337 96-97 23.162735545627402 25.933443333302353 26.72217539695623 24.181645724114013 98-99 23.221550497066072 26.461038087510985 25.994354717125855 24.32305669829709 100 23.218910472397866 25.505919746288765 26.197254696624256 25.077915084689117 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4235.0 1 2431.0 2 627.5 3 583.0 4 504.5 5 464.0 6 435.5 7 399.0 8 383.5 9 385.5 10 382.5 11 401.5 12 418.0 13 430.0 14 469.5 15 538.5 16 627.0 17 726.0 18 934.5 19 1159.0 20 1598.5 21 2234.0 22 3112.0 23 4246.0 24 6005.0 25 8655.0 26 12282.0 27 18339.0 28 27085.0 29 35845.5 30 43658.0 31 51015.5 32 57470.5 33 67143.0 34 75636.0 35 83570.0 36 96883.0 37 112828.5 38 126362.5 39 133844.0 40 143837.5 41 158772.0 42 170535.5 43 179912.5 44 183932.5 45 184360.5 46 191668.0 47 213730.5 48 254005.0 49 252198.5 50 215142.0 51 195700.5 52 178897.0 53 169187.0 54 163487.0 55 156038.5 56 145714.0 57 129492.5 58 116311.0 59 109171.0 60 101507.0 61 89680.5 62 79683.0 63 70393.5 64 58319.5 65 48213.0 66 39325.5 67 30421.5 68 23888.0 69 19308.0 70 14650.0 71 10836.5 72 8528.0 73 6752.5 74 5386.0 75 3996.5 76 2744.0 77 2104.5 78 1532.5 79 961.5 80 640.5 81 375.0 82 200.0 83 140.0 84 104.5 85 92.0 86 75.0 87 51.0 88 39.0 89 33.0 90 28.0 91 24.0 92 21.0 93 16.0 94 9.0 95 8.0 96 9.0 97 3.5 98 4.0 99 4.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07858147436364941 2 0.08114285110951606 3 0.08251152570654403 4 0.08491648249846463 5 0.08288302309716593 6 0.08282436561443615 7 0.08169032094832725 8 0.08178808341954354 9 0.08169032094832725 10-11 0.08117217985088095 12-13 0.08018477889159648 14-15 0.07863035559925756 16-17 0.07731056223783771 18-19 0.07692928860009421 20-21 0.07644047624401279 22-23 0.07808288576044636 24-25 0.07827841070287893 26-27 0.07756474466300005 28-29 0.07351737835464588 30-31 0.07904095797836595 32-33 0.07758429715724331 34-35 0.07444612183120058 36-37 0.07116130279833342 38-39 0.07276460732628048 40-41 0.07594188764080974 42-43 0.08087889243723209 44-45 0.08207159458607076 46-47 0.0855226098200056 48-49 0.08881720509999438 50-51 0.08976550107079236 52-53 0.08996102601322492 54-55 0.09005878848444121 56-57 0.08782980414070991 58-59 0.0867250882159659 60-61 0.08441789389526158 62-63 0.08510223119377558 64-65 0.08429080268268042 66-67 0.08368467536113945 68-69 0.08312742927520662 70-71 0.08113307486239443 72-73 0.08118195609800258 74-75 0.08062471001206975 76-77 0.0804682900581237 78-79 0.0809473261670835 80-81 0.08224756703426007 82-83 0.08150457225301631 84-85 0.0805758287764616 86-87 0.08145569101740817 88-89 0.0784055019154601 90-91 0.07529665533078225 92-93 0.07519889285956596 94-95 0.0756583764742825 96-97 0.07487627670455223 98-99 0.07427014938301127 100 0.07322409094099702 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5114437.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.637227964696965 #Duplication Level Percentage of deduplicated Percentage of total 1 58.73935514220663 13.884355279956331 2 12.789851543254722 6.046332731250863 3 6.697346587955634 4.7492012417427825 4 4.161738900472834 3.9348788448009464 5 3.010837871160578 3.5583930562682746 6 2.299574419997971 3.2613338872366704 7 1.6697323753106175 2.7627491356676415 8 1.3550539924327138 2.562377609888385 9 1.0640526274522955 2.263612906937221 >10 7.387119997811078 32.84180670350453 >50 0.5541364143149654 8.991460886304964 >100 0.2609252494266734 10.637835471165635 >500 0.0072089875512692135 1.1110306899198825 >1k 0.002154409647345945 1.0947935021848287 >5k 5.800333665930995E-4 0.9123866772626702 >10k+ 3.3144763805319975E-4 1.3874513759082288 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 30774 0.6017084578419873 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 16206 0.316867721706221 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12921 0.2526377781171222 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10937 0.2138456295385005 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9130 0.17851427244093535 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7480 0.1462526569395615 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7244 0.14163826829815288 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6295 0.12308295126130207 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5799 0.11338491411664667 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5326 0.10413658433958617 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5309 0.10380419193745079 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.955249424325688E-5 5 0.0 0.0 0.0 0.0 1.955249424325688E-5 6 0.0 0.0 0.0 0.0 1.955249424325688E-5 7 0.0 0.0 0.0 0.0 1.955249424325688E-5 8 0.0 0.0 0.0 0.0 1.955249424325688E-5 9 0.0 0.0 0.0 0.0 1.955249424325688E-5 10-11 0.0 0.0 0.0 0.0 1.955249424325688E-5 12-13 0.0 0.0 0.0 0.0 1.955249424325688E-5 14-15 0.0 0.0 0.0 1.955249424325688E-5 1.955249424325688E-5 16-17 0.0 0.0 0.0 6.843372985139908E-5 1.955249424325688E-5 18-19 0.0 0.0 0.0 1.2709121258116973E-4 1.955249424325688E-5 20-21 0.0 0.0 0.0 1.7597244818931193E-4 1.955249424325688E-5 22-23 0.0 0.0 0.0 3.812736377435092E-4 1.955249424325688E-5 24-25 0.0 0.0 0.0 7.038897927572477E-4 1.955249424325688E-5 26-27 0.0 0.0 0.0 0.0012904646200549543 1.955249424325688E-5 28-29 0.0 0.0 0.0 0.004037590061232546 1.955249424325688E-5 30-31 0.0 0.0 0.0 0.01297307993040094 1.955249424325688E-5 32-33 0.0 0.0 0.0 0.032026985570454775 1.955249424325688E-5 34-35 0.0 0.0 0.0 0.059253833804189984 2.9328741364885322E-5 36-37 0.0 0.0 0.0 0.10238663610481466 3.910498848651376E-5 38-39 0.0 0.0 0.0 0.17082036595621375 3.910498848651376E-5 40-41 0.0 0.0 0.0 0.25045767500899907 3.910498848651376E-5 42-43 0.0 0.0 0.0 0.3325683745835563 3.910498848651376E-5 44-45 0.0 0.0 0.0 0.43322461494784276 3.910498848651376E-5 46-47 0.0 0.0 0.0 0.5571092184731183 3.910498848651376E-5 48-49 0.0 0.0 0.0 0.6858819455592082 3.910498848651376E-5 50-51 0.0 0.0 0.0 0.8203346722229641 3.910498848651376E-5 52-53 0.0 0.0 0.0 0.9578571404829115 3.910498848651376E-5 54-55 0.0 0.0 0.0 1.1116570602003701 3.910498848651376E-5 56-57 0.0 0.0 0.0 1.3059013142600056 3.910498848651376E-5 58-59 0.0 0.0 0.0 1.5097849479815668 3.910498848651376E-5 60-61 0.0 0.0 0.0 1.7216068943658902 3.910498848651376E-5 62-63 0.0 0.0 0.0 1.9411618522234217 3.910498848651376E-5 64-65 0.0 0.0 0.0 2.169085277617067 3.910498848651376E-5 66-67 0.0 0.0 0.0 2.4339335883891033 3.910498848651376E-5 68-69 0.0 0.0 0.0 2.739675940870911 3.910498848651376E-5 70-71 0.0 0.0 0.0 3.0592125780413366 3.910498848651376E-5 72-73 0.0 0.0 0.0 3.3836080100312116 3.910498848651376E-5 74-75 0.0 0.0 0.0 3.720575695819501 3.910498848651376E-5 76-77 0.0 0.0 0.0 4.077154533333776 3.910498848651376E-5 78-79 0.0 0.0 0.0 4.502528821842952 4.88812356081422E-5 80-81 0.0 0.0 0.0 4.952079769483914 5.8657482729770644E-5 82-83 0.0 0.0 0.0 5.398033058184117 5.8657482729770644E-5 84-85 0.0 0.0 0.0 5.847740425779025 5.8657482729770644E-5 86-87 0.0 0.0 0.0 6.313774126067052 5.8657482729770644E-5 88 0.0 0.0 0.0 6.698880052682241 5.8657482729770644E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 21955 0.0 28.472998 1 TACATGG 22310 0.0 27.029753 2 ACATGGG 22700 0.0 25.695726 3 CATGGGG 15680 0.0 22.571682 4 GAGTACA 15440 0.0 18.538942 1 AGTACAT 15245 0.0 17.63533 2 GTATTAG 2670 0.0 17.427654 1 TATACTG 1885 0.0 16.954912 5 CATGGGA 6965 0.0 16.735764 4 AGAGTAC 13690 0.0 15.792091 10-11 ATGGGGG 8850 0.0 15.772887 5 GTATTAA 2065 0.0 15.70522 1 GTACTAG 515 7.8216544E-11 14.602518 1 ATGGGGC 5525 0.0 14.546611 5 ATGGGGA 7060 0.0 14.512753 5 CCCTATA 1885 0.0 14.462108 2 TAGTGCG 260 1.3327076E-4 14.461542 5 ACCTATC 1015 0.0 13.891358 8 CATGGGC 6405 0.0 13.502484 4 GTATATA 1925 0.0 13.429102 1 >>END_MODULE