FastQCFastQC Report
Wed 25 May 2016
SRR1032920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032920_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5114437
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT312710.6114260474808859No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT159810.3124684105014882No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT129020.25226628072650026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114920.2246972638435081No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC110830.21670029369801605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98060.191731758549377No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT88480.1730004690643369No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT77540.15161004036221387No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC73910.14451248495191163No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC63980.12509685816835753No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA57730.11287654926632198No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC52540.10272880475407167No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG52360.10237685985769304No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG228400.026.9241751
TACATGG229100.025.4067382
ACATGGG228100.024.5691873
GAGTACT166000.020.82676912-13
CATGGGG158450.020.3424224
AGTACTT161750.019.76223412-13
GTACTTT173350.019.60514-15
AGAGTAC266800.019.41086610-11
CATGGGA69750.018.8893994
GAGTACA162500.018.646841
AGTACAT161650.017.2034722
ACTTTTT206200.016.60699516-17
TATCGTT2556.0364437E-616.6058047
CTATACC19700.016.4811384
TACTTTT194550.016.43043514-15
GTATTAG25250.016.1866421
TATACTG19900.016.0777825
GTGTAGA23850.015.3640281
CTTAGAC12050.015.2293683
GTACTAG4753.601599E-1014.8353061
Produced by FastQC (version 0.11.5)