FastQCFastQC Report
Wed 25 May 2016
SRR1032919_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032919_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542426
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44700.028.2846931
TCAACGC43750.027.7176784
ATCAACG44250.027.616383
GTACATG79500.027.1363981
CAACGCA44900.027.0066995
AACGCAG46400.026.1336366
TACATGG80800.025.4782412
ACATGGG80250.024.8914723
GGTATCA30550.024.1543351
TATCAAC52150.023.973642
ACGCAGA55200.021.711977
CGCAGAG56250.021.0560138
GAGTACA58250.020.3334621
GTATAGA12650.020.0636481
CATGGGG50100.019.0446744
GTTAGAC4250.018.800383
AGTACAT58400.018.7520922
CATGGGA28900.017.5647164
GCGAGTA2152.6243553E-517.4883829
TTACGGT1902.0234653E-417.3164834
Produced by FastQC (version 0.11.5)