##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032919_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2542426
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48238886795525	33.0	31.0	34.0	30.0	34.0
2	31.543366060605106	34.0	31.0	34.0	30.0	34.0
3	31.68388775130525	34.0	31.0	34.0	30.0	34.0
4	35.01921747181629	37.0	35.0	37.0	33.0	37.0
5	35.06838507787444	37.0	35.0	37.0	33.0	37.0
6	35.20793486221428	37.0	36.0	37.0	33.0	37.0
7	35.091875240419974	37.0	35.0	37.0	33.0	37.0
8	35.10617378834232	37.0	35.0	37.0	33.0	37.0
9	36.75296311475732	39.0	38.0	39.0	33.0	39.0
10-11	36.74627186789311	39.0	38.0	39.0	33.5	39.0
12-13	36.696393326688764	39.0	38.0	39.0	33.0	39.0
14-15	38.1531098643579	41.0	39.0	41.0	34.0	41.0
16-17	38.172561757943	41.0	39.0	41.0	34.0	41.0
18-19	38.178035466912306	41.0	39.0	41.0	34.0	41.0
20-21	38.233157425230864	41.0	39.0	41.0	34.0	41.0
22-23	38.21831058209757	41.0	39.0	41.0	34.0	41.0
24-25	38.2567950846947	41.0	39.0	41.0	34.0	41.0
26-27	38.24842296294956	41.0	39.0	41.0	34.0	41.0
28-29	38.23563793007151	41.0	39.0	41.0	33.0	41.0
30-31	38.18752758192372	41.0	39.0	41.0	33.0	41.0
32-33	38.148932358306595	41.0	39.0	41.0	33.0	41.0
34-35	38.07846187066998	41.0	39.0	41.0	33.0	41.0
36-37	37.981934970772016	41.0	38.0	41.0	33.0	41.0
38-39	37.884690842526	40.5	38.0	41.0	33.0	41.0
40-41	37.7999550429393	40.0	38.0	41.0	33.0	41.0
42-43	37.680085870739205	40.0	38.0	41.0	32.0	41.0
44-45	37.52774712027016	40.0	38.0	41.0	32.0	41.0
46-47	37.39673327758605	40.0	38.0	41.0	31.5	41.0
48-49	37.25709519175779	40.0	37.5	41.0	31.0	41.0
50-51	37.114097126130716	40.0	37.0	41.0	31.0	41.0
52-53	36.96088067066652	40.0	37.0	41.0	31.0	41.0
54-55	36.79325553624766	40.0	36.0	41.0	30.0	41.0
56-57	36.61085416055374	40.0	36.0	41.0	30.0	41.0
58-59	36.402748201914235	39.0	35.0	41.0	30.0	41.0
60-61	36.15692492131531	39.0	35.0	41.0	30.0	41.0
62-63	35.90129879886376	39.0	35.0	41.0	30.0	41.0
64-65	35.62323524853821	38.0	35.0	40.0	29.0	41.0
66-67	35.32264970543882	37.5	35.0	40.0	29.0	41.0
68-69	35.00932986840128	37.0	34.5	40.0	29.0	41.0
70-71	34.67039728983262	36.5	34.0	39.5	29.0	41.0
72-73	34.37977663853343	36.0	34.0	39.0	28.5	41.0
74-75	34.10612599934079	36.0	34.0	39.0	28.0	40.5
76-77	33.782955334786536	35.0	34.0	38.5	28.0	40.0
78-79	33.415876804280636	35.0	34.0	37.0	28.0	39.0
80-81	33.09809705375889	35.0	34.0	37.0	27.0	39.0
82-83	32.78006911508929	35.0	33.5	36.0	27.0	38.5
84-85	32.619614494187836	35.0	34.0	36.0	27.0	37.0
86-87	32.39174611178457	35.0	34.0	36.0	27.0	37.0
88-89	32.16111147384427	35.0	33.0	35.0	26.5	37.0
90-91	31.96684052947854	35.0	33.0	35.0	26.0	36.0
92-93	31.779425045212722	35.0	33.0	35.0	26.0	36.0
94-95	31.602053117770193	35.0	33.0	35.0	25.0	36.0
96-97	31.434536147758084	35.0	33.0	35.0	25.0	36.0
98-99	31.254019782680004	35.0	33.0	35.0	24.5	35.0
100	31.124388674439295	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3641362600602811
1101	2	0.47702825735154875
1101	3	0.45494729106572507
1101	4	0.4154422241946705
1101	5	0.30651275468103023
1101	6	0.3034878509603658
1101	7	0.3610728889515542
1101	8	0.34047514719202354
1101	9	0.42516607450397004
1101	10-11	0.37519536380994367
1101	12-13	0.17306778795598632
1101	14-15	0.2560122980641495
1101	16-17	0.27296741016660775
1101	18-19	0.2663602738769484
1101	20-21	0.23038512996521376
1101	22-23	0.256559630767498
1101	24-25	0.26776698225901185
1101	26-27	0.3660493703846939
1101	28-29	0.393523440760994
1101	30-31	0.35303393366559277
1101	32-33	0.3527196449336927
1101	34-35	0.3889674306904425
1101	36-37	0.33916266978138054
1101	38-39	0.337618160380039
1101	40-41	0.3643606433157487
1101	42-43	0.3668405458969204
1101	44-45	0.253749786844196
1101	46-47	0.2445126853997266
1101	48-49	0.31266748519050935
1101	50-51	0.33549729551553753
1101	52-53	0.26847306236071233
1101	54-55	0.2934268525703345
1101	56-57	0.2929630156028864
1101	58-59	0.23968110004724252
1101	60-61	0.2915511704932783
1101	62-63	0.27919341271186227
1101	64-65	0.30623204611385546
1101	66-67	0.19361775886081034
1101	68-69	0.18691423961530518
1101	70-71	0.23465735086349326
1101	72-73	0.22491683113102567
1101	74-75	0.2130525240376926
1101	76-77	0.2117178367384298
1101	78-79	0.2074603079330828
1101	80-81	0.20055639322828966
1101	82-83	0.1580446402236788
1101	84-85	0.157534944470342
1101	86-87	0.16611546559456514
1101	88-89	0.2268573925142121
1101	90-91	0.2887863270153197
1101	92-93	0.40021185018725447
1101	94-95	0.2872665164357038
1101	96-97	0.2885188372972607
1101	98-99	0.2774888388974155
1101	100	0.29377668506231913
1102	1	0.31123119010391065
1102	2	0.3179394453561635
1102	3	0.27823799865311116
1102	4	0.23803487250719257
1102	5	0.16802571792258902
1102	6	0.19031749842037016
1102	7	0.2195920203892996
1102	8	0.1788413274237044
1102	9	0.19370727103373042
1102	10-11	0.18080666715481186
1102	12-13	0.22580987711901912
1102	14-15	0.28058654208400924
1102	16-17	0.3290323015963281
1102	18-19	0.26622763258513515
1102	20-21	0.31667493488974685
1102	22-23	0.31135151683138673
1102	24-25	0.3360351232750105
1102	26-27	0.3298962185868888
1102	28-29	0.3521115256112992
1102	30-31	0.38286267846274313
1102	32-33	0.3504118369000011
1102	34-35	0.38499370820912304
1102	36-37	0.3891531416734608
1102	38-39	0.3598346991694612
1102	40-41	0.4247991870196728
1102	42-43	0.4433113848718975
1102	44-45	0.40672381021313697
1102	46-47	0.4155008504867297
1102	48-49	0.39708351247098506
1102	50-51	0.4194098375144364
1102	52-53	0.3995148857396842
1102	54-55	0.3271816793092057
1102	56-57	0.313664835972979
1102	58-59	0.3538626857249909
1102	60-61	0.3669671476258216
1102	62-63	0.41090170216574506
1102	64-65	0.3870110943060254
1102	66-67	0.3388537049016378
1102	68-69	0.3558563688966814
1102	70-71	0.37008135166585987
1102	72-73	0.31800343661721087
1102	74-75	0.3046878482441002
1102	76-77	0.33298708100903696
1102	78-79	0.3521960283081782
1102	80-81	0.26104934744322605
1102	82-83	0.30674831606741293
1102	84-85	0.3076623202288289
1102	86-87	0.25048059271959033
1102	88-89	0.18705597834240706
1102	90-91	0.27561948483087306
1102	92-93	0.3219926034092637
1102	94-95	0.36616276035526596
1102	96-97	0.34075919635648333
1102	98-99	0.2893227489320154
1102	100	0.3503119641355852
1103	1	0.20530009314233055
1103	2	0.33919910595600555
1103	3	0.36268937153380776
1103	4	0.2867556427602693
1103	5	0.2562749133535931
1103	6	0.2699218654163431
1103	7	0.2655994331223823
1103	8	0.27195134875856297
1103	9	0.41926412838089533
1103	10-11	0.37062060579847866
1103	12-13	0.37613811070269776
1103	14-15	0.3651448575244842
1103	16-17	0.40627263237929867
1103	18-19	0.44634617745743554
1103	20-21	0.44080622474626807
1103	22-23	0.378098510326879
1103	24-25	0.3626300835530216
1103	26-27	0.3996493507722789
1103	28-29	0.47398576246171586
1103	30-31	0.4502486446072709
1103	32-33	0.4846020276780436
1103	34-35	0.41580162002374976
1103	36-37	0.44318006762319584
1103	38-39	0.4804157480536375
1103	40-41	0.4976232493964119
1103	42-43	0.4621320966256448
1103	44-45	0.4152767355209761
1103	46-47	0.4482801067355666
1103	48-49	0.42842593384889227
1103	50-51	0.4933042603725539
1103	52-53	0.4188810004835659
1103	54-55	0.4255257890056896
1103	56-57	0.4162990047833759
1103	58-59	0.40523929783022083
1103	60-61	0.4161421087427897
1103	62-63	0.5000473697198231
1103	64-65	0.40235785972242155
1103	66-67	0.4297530109415675
1103	68-69	0.4310243878115756
1103	70-71	0.32605841238516575
1103	72-73	0.31648030792096904
1103	74-75	0.29510553474688805
1103	76-77	0.24840750712736792
1103	78-79	0.3427078941766055
1103	80-81	0.318889379258529
1103	82-83	0.28809698084219804
1103	84-85	0.23101251073371998
1103	86-87	0.23077247314943605
1103	88-89	0.30210820663351257
1103	90-91	0.30984053489868657
1103	92-93	0.30061059499303155
1103	94-95	0.34807503802774065
1103	96-97	0.30026503637139257
1103	98-99	0.3714630894377784
1103	100	0.4424193837299022
1104	1	-0.180422369715739
1104	2	-0.13211774815809108
1104	3	-0.10187867611596957
1104	4	-0.10397567016393339
1104	5	-0.10057551936376541
1104	6	-0.03886692206634734
1104	7	0.008027015194222997
1104	8	-0.04367385268820101
1104	9	0.005333759256636483
1104	10-11	-0.026567721589501048
1104	12-13	-0.01877913981979873
1104	14-15	-0.03249051664419511
1104	16-17	-0.04815368217978033
1104	18-19	-0.10116038728007481
1104	20-21	-0.07084615521863924
1104	22-23	-0.07327987096923039
1104	24-25	-0.09544537850275958
1104	26-27	0.005363060646999429
1104	28-29	0.021990323022343716
1104	30-31	0.002058966603662782
1104	32-33	0.04141529710761205
1104	34-35	0.06546641260445085
1104	36-37	0.04155097975861821
1104	38-39	0.023560189365547046
1104	40-41	-0.007262305540407965
1104	42-43	0.07359225268815095
1104	44-45	-0.08918709182390216
1104	46-47	-0.046845561096866106
1104	48-49	-0.03153421561197689
1104	50-51	0.020782958469183654
1104	52-53	-0.003143897754270597
1104	54-55	-0.02871711676731792
1104	56-57	-0.04575898703215131
1104	58-59	-0.07345460482759592
1104	60-61	0.029134114558438284
1104	62-63	-0.04060177234023854
1104	64-65	-0.05329484333397971
1104	66-67	-0.07434846463900158
1104	68-69	-0.060079412319055336
1104	70-71	-0.004597649735387677
1104	72-73	-0.026668987529888
1104	74-75	-0.030910824866367648
1104	76-77	-0.054090044444947694
1104	78-79	-0.0793345177945568
1104	80-81	-0.09919687063564453
1104	82-83	-0.09524916741796119
1104	84-85	-0.1402839559967859
1104	86-87	-0.12815929785015356
1104	88-89	-0.13507457538613465
1104	90-91	-0.11441645176058657
1104	92-93	-0.13743098128956888
1104	94-95	-0.12785146176527817
1104	96-97	-0.15949888937659296
1104	98-99	-0.16331005917421137
1104	100	-0.21844274241402317
1105	1	0.07055514739080948
1105	2	0.10154438638380725
1105	3	0.15107164824197739
1105	4	0.11255769871547727
1105	5	0.08901392345148196
1105	6	0.21044437269949157
1105	7	0.16165493663739028
1105	8	0.15332694709852035
1105	9	0.2287986130733941
1105	10-11	0.21791041757337837
1105	12-13	0.2318429165207334
1105	14-15	0.33352632330959153
1105	16-17	0.2920522629015352
1105	18-19	0.35213984376942875
1105	20-21	0.29914740205497736
1105	22-23	0.3274665966394039
1105	24-25	0.2986295348536885
1105	26-27	0.3241980379863776
1105	28-29	0.33915574557632766
1105	30-31	0.3408541767698452
1105	32-33	0.4100980928065354
1105	34-35	0.41149080609952193
1105	36-37	0.3727659890894657
1105	38-39	0.3625386839892002
1105	40-41	0.3158426442507647
1105	42-43	0.2984300737136536
1105	44-45	0.2961445975033854
1105	46-47	0.24069664986910055
1105	48-49	0.2585873075374039
1105	50-51	0.23176891776893171
1105	52-53	0.25659017316015564
1105	54-55	0.25886397271993644
1105	56-57	0.33366427787498054
1105	58-59	0.2799300481538296
1105	60-61	0.3087928722230586
1105	62-63	0.30445287975253166
1105	64-65	0.30881970389787483
1105	66-67	0.16705160645314265
1105	68-69	0.2263953055339556
1105	70-71	0.2089198567073396
1105	72-73	0.21426231967707565
1105	74-75	0.25710348242946424
1105	76-77	0.24234312893104715
1105	78-79	0.22994745145809503
1105	80-81	0.2471541024381949
1105	82-83	0.276341741672951
1105	84-85	0.19574088182424276
1105	86-87	0.15876974329443527
1105	88-89	0.1994462190686761
1105	90-91	0.1517059156081153
1105	92-93	0.24444163045841094
1105	94-95	0.24766506716034087
1105	96-97	0.26379934220609513
1105	98-99	0.273483462553326
1105	100	0.25236948543632565
1106	1	-0.28520000387726085
1106	2	-0.2583377247442904
1106	3	-0.2179519465192712
1106	4	-0.19026466959382304
1106	5	-0.30002020316864275
1106	6	-0.2102507299861287
1106	7	-0.14138698695567342
1106	8	-0.16300677693280363
1106	9	-0.1660705030998031
1106	10-11	-0.12218220333065943
1106	12-13	-0.09433130080927299
1106	14-15	-0.08307303288806622
1106	16-17	-0.10805624072516906
1106	18-19	-0.14505626114148384
1106	20-21	-0.16748422919406636
1106	22-23	-0.11867653472005202
1106	24-25	-0.08661014987002602
1106	26-27	-0.1409270703049188
1106	28-29	-0.12256031041239623
1106	30-31	-0.10994017169878845
1106	32-33	-0.06690487140213719
1106	34-35	-0.12729467522341764
1106	36-37	-0.11175154135950294
1106	38-39	-0.1022428648735314
1106	40-41	-0.027876460880968068
1106	42-43	8.277702942010023E-4
1106	44-45	-0.07591433843912654
1106	46-47	-0.09876066212872558
1106	48-49	-0.0782595473437766
1106	50-51	-0.0505388333394734
1106	52-53	-0.05943842423405954
1106	54-55	-0.008567103058489067
1106	56-57	0.003735962462791065
1106	58-59	-0.06781048591332706
1106	60-61	-0.0359921589478418
1106	62-63	-0.1244986906835237
1106	64-65	-0.12744174100522088
1106	66-67	-0.09571529972837567
1106	68-69	-0.05832925238277653
1106	70-71	-0.09821738870195418
1106	72-73	-0.05774841602422498
1106	74-75	-0.10909986738113986
1106	76-77	-0.07276690351387316
1106	78-79	-0.07701206883272249
1106	80-81	-0.124325581266298
1106	82-83	-0.10548421935916963
1106	84-85	-0.04141003801471754
1106	86-87	-0.08111648714943698
1106	88-89	-0.07280169579313167
1106	90-91	-0.08043706286951746
1106	92-93	-0.07550626232047364
1106	94-95	-0.14699659687336109
1106	96-97	-0.13781653372181069
1106	98-99	-0.15762437858547074
1106	100	-0.12339619769061727
1107	1	0.12162161155034923
1107	2	0.16988393800661683
1107	3	0.1826974282796172
1107	4	0.19720182545852083
1107	5	0.162434312837469
1107	6	0.1655450209424174
1107	7	0.20364242301768343
1107	8	0.19373757432031624
1107	9	0.19714091615172435
1107	10-11	0.16826769407265374
1107	12-13	0.22042905358723885
1107	14-15	0.24529856332456035
1107	16-17	0.1576958008330962
1107	18-19	0.18837665054019936
1107	20-21	0.22563552677295462
1107	22-23	0.18643799250781967
1107	24-25	0.16503316237263732
1107	26-27	0.24641384014571344
1107	28-29	0.21208128568704154
1107	30-31	0.24855178630529196
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2216	18-19	0.13440515950491516
2216	20-21	0.14862954897280645
2216	22-23	0.17805319128115826
2216	24-25	0.16513945916256034
2216	26-27	0.1557572009752235
2216	28-29	0.1457657831202397
2216	30-31	0.0909873091573914
2216	32-33	0.11434724934469642
2216	34-35	0.09438691991001491
2216	36-37	0.1443163229936104
2216	38-39	0.17363212146164386
2216	40-41	0.1833966281286905
2216	42-43	0.21341933030704752
2216	44-45	0.2504345929090164
2216	46-47	0.30090213202298344
2216	48-49	0.3475624956050254
2216	50-51	0.35469472097199173
2216	52-53	0.40235551906071976
2216	54-55	0.36485661244222456
2216	56-57	0.4258095250197371
2216	58-59	0.45892268555574134
2216	60-61	0.45246864874164316
2216	62-63	0.45631406472939773
2216	64-65	0.42938961493411654
2216	66-67	0.4561226846456492
2216	68-69	0.40330162210015175
2216	70-71	0.33579525407416355
2216	72-73	0.3536156733370319
2216	74-75	0.3474085441286334
2216	76-77	0.33540626442791677
2216	78-79	0.3837308476739949
2216	80-81	0.39431562712019286
2216	82-83	0.38934166974085826
2216	84-85	0.3821887008895004
2216	86-87	0.4441649173469031
2216	88-89	0.5156160321844538
2216	90-91	0.4839557427390453
2216	92-93	0.42662306408828954
2216	94-95	0.47048732295087703
2216	96-97	0.4855739998325248
2216	98-99	0.46886849650063667
2216	100	0.44095200547289437
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	38242.0
3	5486.0
4	3608.0
5	3150.0
6	2505.0
7	2897.0
8	3160.0
9	3428.0
10	3660.0
11	4049.0
12	4371.0
13	4885.0
14	5246.0
15	5485.0
16	5355.0
17	5410.0
18	5227.0
19	5053.0
20	5211.0
21	5425.0
22	6257.0
23	8929.0
24	13617.0
25	18643.0
26	20037.0
27	17173.0
28	17720.0
29	22595.0
30	27387.0
31	32913.0
32	42435.0
33	57112.0
34	84914.0
35	132307.0
36	250380.0
37	520358.0
38	811466.0
39	335824.0
40	506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.648864559153886	19.414186446822573	13.07783175298085	23.85911724104269
2	15.520672146383305	19.902857779427563	40.37747613662263	24.198993937566502
3	18.66268510069777	25.228608071095042	29.179404292438832	26.929302535768358
4	12.198530413265175	16.38822252122382	38.68636298146759	32.72688408404342
5	12.909734911796036	38.24407702524286	35.34441154449641	13.501776518464691
6	31.395073285061848	37.75304544169828	17.719459571382572	13.1324217018573
7	27.953869730051238	32.21671165353407	22.05189714415729	17.777521472257398
8	25.420743146907597	36.62404059855471	19.85623444626132	18.098981808276378
9	25.16589998527783	17.20644028112263	20.68843081327011	36.939228920329434
10-11	24.10652533409227	27.038672393344175	28.43576047939077	20.41904179317278
12-13	24.637467227438865	24.395283388550464	28.49550203302557	22.4717473509851
14-15	21.918148465310786	26.371918847156163	25.510049240975718	26.199883446557333
16-17	21.814032670734107	29.711237945286364	26.107341074591616	22.367388309387913
18-19	21.732270292185426	28.769892659347928	27.955469571070374	21.542367477396272
20-21	22.785516899201127	28.075202729205586	27.46091891835508	21.678361453238207
22-23	22.449144272853342	27.77246817617678	27.613757448914	22.16463010205588
24-25	22.24238689550345	27.928634507413292	27.606745440757752	22.222233156325508
26-27	22.01895422868476	27.912466441001406	27.64364018967218	22.424939140641655
28-29	22.07124628751331	27.92230833767781	27.785410544790633	22.221034830018247
30-31	22.276855820767857	28.355697069762382	27.356842335018527	22.010604774451238
32-33	21.9114207186205	28.052395352475944	27.80592739399939	22.230256534904157
34-35	22.113331394133674	27.826721357870117	27.894737670764048	22.16520957723217
36-37	22.360067659917405	28.166658302657098	27.327876063090216	22.145397974335282
38-39	22.21222241093504	28.27993427545857	27.513876132186706	21.99396718141969
40-41	21.998147625508608	28.011712548951532	27.81651652041725	22.173623305122607
42-43	21.93491423798281	27.981122672710857	27.69150315579975	22.392459933506586
44-45	21.978024140890817	28.03969959594604	27.790622539121628	22.191653724041515
46-47	22.14613080973943	28.17266121913126	27.60965518734555	22.07155278378376
48-49	22.01085897465675	28.06868191242983	27.656037726118736	22.264421386794677
50-51	21.995103092326925	27.969653265946885	27.678669319765238	22.356574321960952
52-53	22.092900347161326	27.914884554145615	27.684939631092124	22.307275467600935
54-55	22.11014721884099	28.31598668432432	27.536529137488763	22.037336959345932
56-57	22.00171283736371	28.06592868872675	27.59943540534428	22.33292306856526
58-59	21.933305527731363	27.642416284503664	27.915282537524277	22.508995650240692
60-61	22.145996087503097	28.04654843884918	27.62197619474543	22.185479278902296
62-63	21.919394969217223	28.121062842762107	27.57181679883935	22.38772538918132
64-65	21.93772259679116	27.897505341338864	27.695759700274863	22.46901236159511
66-67	21.846674005563415	27.89472927993456	27.584397476642447	22.674199237859575
68-69	22.188456129611932	28.00902630638858	27.55971858236209	22.242798981637392
70-71	22.15642592464661	27.814120243668704	27.6863065564249	22.343147275259785
72-73	21.97666868537326	27.906765278220348	27.7454721303351	22.371093906071295
74-75	21.973320201667125	27.948700689333748	27.641685010061313	22.436294098937815
76-77	21.981192903231904	27.909888270619394	27.521449175413238	22.587469650735468
78-79	22.1427751818459	27.9942402918588	27.605227746031986	22.257756780263314
80-81	22.03689765853743	27.80818445129032	27.755239574582998	22.399678315589256
82-83	21.976842573072805	27.849769002412817	27.837664583197608	22.33572384131677
84-85	21.971130313131848	27.831884193747047	27.61583685818324	22.58114863493787
86-87	22.13451929110209	27.880931068172888	27.619278741801466	22.365270898923555
88-89	21.891981362496452	27.805436712669984	27.690202084733173	22.61237984010039
90-91	21.905810987858686	27.69595682493548	27.74679246404456	22.651439723161275
92-93	22.09160486226955	27.832564862143588	27.618417644738802	22.457412630848065
94-95	21.894030194427554	27.613691604327435	27.77548823377753	22.71678996746748
96-97	22.018507333322706	27.616858563297352	27.725456646279433	22.63917745710051
98-99	21.912668623178366	27.696790019271223	27.698226701346467	22.692314656203948
100	22.057527514245688	27.562029809575954	27.573523189271164	22.80691948690719
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2286.0
1	1408.0
2	459.0
3	366.0
4	308.0
5	251.0
6	225.0
7	229.0
8	231.0
9	236.5
10	250.5
11	238.0
12	232.0
13	264.0
14	308.5
15	361.0
16	449.0
17	586.0
18	829.0
19	1106.5
20	1559.5
21	2323.0
22	3359.5
23	5066.5
24	7680.0
25	10738.0
26	14562.0
27	19562.0
28	25787.0
29	33152.0
30	40442.5
31	47094.5
32	53188.5
33	59262.5
34	65174.0
35	70115.5
36	74896.5
37	79628.0
38	83258.0
39	85223.0
40	87055.0
41	88919.0
42	89725.5
43	90474.5
44	89917.5
45	89036.0
46	88954.0
47	87880.0
48	86571.5
49	84694.0
50	81791.5
51	78532.5
52	73636.0
53	69598.5
54	66819.0
55	63241.5
56	59267.0
57	54482.5
58	49639.5
59	45454.5
60	40822.0
61	35126.5
62	29866.0
63	25079.0
64	20337.5
65	16490.0
66	13383.0
67	10399.5
68	7987.5
69	6252.0
70	4726.5
71	3533.0
72	2842.5
73	2209.5
74	1661.5
75	1190.0
76	813.5
77	649.5
78	493.0
79	348.5
80	238.0
81	151.5
82	114.5
83	101.5
84	78.5
85	56.5
86	49.0
87	34.0
88	29.5
89	28.0
90	21.5
91	17.5
92	12.5
93	9.5
94	9.0
95	7.0
96	2.5
97	0.5
98	2.0
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07705239011872912
2	0.07972700090386112
3	0.08071031369251258
4	0.08338492447764458
5	0.08118230383106528
6	0.08149696392343375
7	0.08082831122715077
8	0.08023832355395988
9	0.08023832355395988
10-11	0.08004166099622959
12-13	0.07868468934789055
14-15	0.07711138888604821
16-17	0.07575441723770919
18-19	0.07536109212224859
20-21	0.07498743326256103
22-23	0.07689506007254489
24-25	0.07687539381677186
26-27	0.07620674112048885
28-29	0.07254881754670539
30-31	0.07734738395532456
32-33	0.07604941107430463
34-35	0.07311913896412324
36-37	0.06995287178466551
38-39	0.0716835022926921
40-41	0.07484976947214983
42-43	0.07960900336922294
44-45	0.08053331739055533
46-47	0.08391591338351638
48-49	0.08743617316688863
50-51	0.0885571497459513
52-53	0.08859648225749736
54-55	0.08897014111718493
56-57	0.08639386161091808
58-59	0.08537121631072055
60-61	0.08277527054868067
62-63	0.08360125329114791
64-65	0.08253927547940432
66-67	0.08183129027157526
68-69	0.08155596269075285
70-71	0.07903868195180509
72-73	0.07947133957881172
74-75	0.07874368811520965
76-77	0.07874368811520965
78-79	0.07943200706726568
80-81	0.08055298364632836
82-83	0.07990399720581838
84-85	0.07907801446335115
86-87	0.07992366346159141
88-89	0.076973725095637
90-91	0.07416145052009379
92-93	0.0741024517527747
94-95	0.07437777933359711
96-97	0.07337480028917262
98-99	0.07276514636020871
100	0.07209649366392572
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2542426.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.07760832587836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.6993736901314	52.00266390188438
2	13.16287848069915	17.395430573775425
3	3.8475005149987362	7.627008961911054
4	1.5898263702395221	4.202076967953601
5	0.7984351443444035	2.6379342370802825
6	0.4692559935314724	1.8604388247086125
7	0.290218052857594	1.342384037807621
8	0.21834867179836953	1.1542366410854739
9	0.1450415323618443	0.8625597809752044
>10	0.736287224551043	8.45287945705091
>50	0.03404091556392518	1.5136162853504689
>100	0.008614753389702549	0.8271774880424746
>500	1.1910368859411452E-4	0.05485450558906513
>1k	5.9551844296983495E-5	0.06673833678547447
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.933251154605876E-5	0.0
5	0.0	0.0	0.0	3.933251154605876E-5	0.0
6	0.0	0.0	0.0	7.866502309211753E-5	0.0
7	0.0	0.0	0.0	7.866502309211753E-5	0.0
8	0.0	0.0	0.0	7.866502309211753E-5	0.0
9	3.933251154605876E-5	0.0	0.0	7.866502309211753E-5	0.0
10-11	3.933251154605876E-5	0.0	0.0	1.1799753463817629E-4	0.0
12-13	3.933251154605876E-5	0.0	0.0	1.1799753463817629E-4	0.0
14-15	3.933251154605876E-5	0.0	0.0	1.1799753463817629E-4	0.0
16-17	3.933251154605876E-5	5.8998767319088144E-5	0.0	1.1799753463817629E-4	0.0
18-19	3.933251154605876E-5	7.866502309211753E-5	0.0	1.3766379041120567E-4	0.0
20-21	3.933251154605876E-5	7.866502309211753E-5	0.0	1.7699630195726443E-4	0.0
22-23	3.933251154605876E-5	7.866502309211753E-5	0.0	3.146600923684701E-4	0.0
24-25	3.933251154605876E-5	7.866502309211753E-5	0.0	5.506551616448227E-4	0.0
26-27	3.933251154605876E-5	7.866502309211753E-5	0.0	7.669839751481459E-4	0.0
28-29	3.933251154605876E-5	7.866502309211753E-5	0.0	0.0015733004618423505	0.0
30-31	3.933251154605876E-5	7.866502309211753E-5	0.0	0.005231224035625816	0.0
32-33	3.933251154605876E-5	7.866502309211753E-5	0.0	0.012665068717830921	0.0
34-35	3.933251154605876E-5	7.866502309211753E-5	0.0	0.023933833275776757	0.0
36-37	3.933251154605876E-5	1.3766379041120567E-4	0.0	0.043954081652720675	0.0
38-39	3.933251154605876E-5	1.9666255773029381E-4	0.0	0.08004166099622959	0.0
40-41	3.933251154605876E-5	1.9666255773029381E-4	0.0	0.12541171306460835	0.0
42-43	3.933251154605876E-5	1.9666255773029381E-4	0.0	0.1699361161347469	0.0
44-45	3.933251154605876E-5	1.9666255773029381E-4	0.0	0.22132404246967266	0.0
46-47	3.933251154605876E-5	1.9666255773029381E-4	0.0	0.2861046889860315	0.0
48-49	3.933251154605876E-5	1.9666255773029381E-4	0.0	0.3601874744830331	0.0
50-51	3.933251154605876E-5	1.9666255773029381E-4	0.0	0.4447523743070595	0.0
52-53	3.933251154605876E-5	1.9666255773029381E-4	0.0	0.5330341964721884	0.0
54-55	3.933251154605876E-5	1.9666255773029381E-4	0.0	0.6222403326586496	0.0
56-57	3.933251154605876E-5	1.9666255773029381E-4	0.0	0.7287331076696038	0.0
58-59	3.933251154605876E-5	1.9666255773029381E-4	0.0	0.8481466127234382	0.0
60-61	3.933251154605876E-5	1.9666255773029381E-4	0.0	0.9808348404240674	0.0
62-63	3.933251154605876E-5	1.9666255773029381E-4	0.0	1.1188526234391876	0.0
64-65	3.933251154605876E-5	1.9666255773029381E-4	0.0	1.2605873287954104	0.0
66-67	3.933251154605876E-5	1.9666255773029381E-4	0.0	1.423030601480633	0.0
68-69	3.933251154605876E-5	1.9666255773029381E-4	0.0	1.6162318981948738	0.0
70-71	3.933251154605876E-5	1.9666255773029381E-4	0.0	1.8226489187885901	0.0
72-73	3.933251154605876E-5	1.9666255773029381E-4	0.0	2.0372077692723405	0.0
74-75	7.866502309211753E-5	1.9666255773029381E-4	0.0	2.2485610200650874	0.0
76-77	7.866502309211753E-5	2.3599506927635258E-4	0.0	2.4720286844140205	0.0
78-79	7.866502309211753E-5	2.3599506927635258E-4	0.0	2.734337203914686	0.0
80-81	7.866502309211753E-5	2.3599506927635258E-4	0.0	3.026066442051804	0.0
82-83	7.866502309211753E-5	2.3599506927635258E-4	0.0	3.3297134311873777	0.0
84-85	7.866502309211753E-5	2.3599506927635258E-4	0.0	3.622366983345828	0.0
86-87	7.866502309211753E-5	2.3599506927635258E-4	0.0	3.9283739231741652	0.0
88	7.866502309211753E-5	2.3599506927635258E-4	0.0	4.177978041445454	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4470	0.0	28.284693	1
TCAACGC	4375	0.0	27.717678	4
ATCAACG	4425	0.0	27.61638	3
GTACATG	7950	0.0	27.136398	1
CAACGCA	4490	0.0	27.006699	5
AACGCAG	4640	0.0	26.133636	6
TACATGG	8080	0.0	25.478241	2
ACATGGG	8025	0.0	24.891472	3
GGTATCA	3055	0.0	24.154335	1
TATCAAC	5215	0.0	23.97364	2
ACGCAGA	5520	0.0	21.71197	7
CGCAGAG	5625	0.0	21.056013	8
GAGTACA	5825	0.0	20.333462	1
GTATAGA	1265	0.0	20.063648	1
CATGGGG	5010	0.0	19.044674	4
GTTAGAC	425	0.0	18.80038	3
AGTACAT	5840	0.0	18.752092	2
CATGGGA	2890	0.0	17.564716	4
GCGAGTA	215	2.6243553E-5	17.488382	9
TTACGGT	190	2.0234653E-4	17.316483	4
>>END_MODULE