FastQCFastQC Report
Wed 25 May 2016
SRR1032919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032919_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542426
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58760.23111783784464132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46440.1826601836198969No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25590.10065189704636437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48650.041.4263731
GTATCAA82000.036.952881
ATCAACG96450.030.7241043
TCAACGC96600.030.62774
CAACGCA98400.030.0668475
TATCAAC101600.029.397632
AACGCAG101850.029.1869286
GTACATG80550.025.0787181
ACGCAGA118000.025.0721997
CGCAGAG120150.024.466968
TACATGG80850.023.7362692
TAACGCA2052.9649527E-822.9449464
ACATGGG81800.022.6560293
GCAGAGT131700.022.1513279
TAGCGCG1503.273379E-521.9506654
CCTATAC10250.021.5682493
GTACCGT2458.431016E-921.11836
GAGTACT80050.020.27552412-13
AGAGTAC112950.019.90128310-11
CAGAGTA127550.018.83868410-11
Produced by FastQC (version 0.11.5)