FastQCFastQC Report
Wed 25 May 2016
SRR1032918_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032918_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249953
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG608.8245666E-539.1666349
GGTATCA11200.034.8303261
GTATCAA18350.032.7847141
TCAACGC18950.030.754594
CAACGCA19900.029.5225855
ATCAACG19800.029.4343193
AACGCAG20750.028.0867236
GTACATG41350.027.961281
TAGACCG700.00883634626.8571195
GTACACG1606.917071E-826.4374771
GAGTACG1258.024694E-626.3199771
TACATGG42800.026.2453062
TATCAAC23050.025.8958552
ACATGGG42400.024.7193183
ACGCAGA25650.022.721237
TACACGG1903.6403253E-722.2631382
TCTGTCG3200.022.031238
CGCAGAG26800.021.3955048
TACCTGG9850.020.0405922
CATGGGG28100.019.569384
Produced by FastQC (version 0.11.5)