##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032918_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1249953 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40614407101707 33.0 31.0 34.0 30.0 34.0 2 31.54207958219229 34.0 31.0 34.0 30.0 34.0 3 31.721293520636376 34.0 31.0 34.0 30.0 34.0 4 35.02400090243393 37.0 35.0 37.0 33.0 37.0 5 35.128647237136114 37.0 35.0 37.0 33.0 37.0 6 35.17108243269947 37.0 37.0 37.0 33.0 37.0 7 35.08324553003193 37.0 36.0 37.0 33.0 37.0 8 35.09862290822135 37.0 36.0 37.0 33.0 37.0 9 36.726639321638494 39.0 38.0 39.0 34.0 39.0 10-11 36.71933744708801 39.0 38.0 39.0 33.5 39.0 12-13 36.67250728627396 39.0 38.0 39.0 33.0 39.0 14-15 38.149505221396325 41.0 39.0 41.0 34.0 41.0 16-17 38.17312610954172 41.0 39.0 41.0 34.0 41.0 18-19 38.18555057670169 41.0 39.0 41.0 34.0 41.0 20-21 38.22001107241633 41.0 39.0 41.0 34.0 41.0 22-23 38.21871302360969 41.0 39.0 41.0 34.0 41.0 24-25 38.25812730558669 41.0 39.0 41.0 34.0 41.0 26-27 38.2477773164271 41.0 39.0 41.0 33.5 41.0 28-29 38.235538456245955 41.0 39.0 41.0 33.0 41.0 30-31 38.19272884660464 41.0 39.0 41.0 33.0 41.0 32-33 38.14051168323929 41.0 39.0 41.0 33.0 41.0 34-35 38.07777372429203 41.0 39.0 41.0 33.0 41.0 36-37 37.98643348989923 41.0 38.5 41.0 33.0 41.0 38-39 37.885081679071135 41.0 38.0 41.0 33.0 41.0 40-41 37.78821523689291 40.5 38.0 41.0 32.5 41.0 42-43 37.674896576111266 40.0 38.0 41.0 32.0 41.0 44-45 37.53842104463128 40.0 38.0 41.0 32.0 41.0 46-47 37.41008021901624 40.0 38.0 41.0 31.5 41.0 48-49 37.27472592969495 40.0 37.5 41.0 31.0 41.0 50-51 37.131404940825774 40.0 37.0 41.0 31.0 41.0 52-53 36.96693435673181 40.0 37.0 41.0 30.5 41.0 54-55 36.79505629411666 40.0 36.0 41.0 30.0 41.0 56-57 36.613022249636586 40.0 36.0 41.0 30.0 41.0 58-59 36.40540604326723 39.5 35.0 41.0 30.0 41.0 60-61 36.1623045026493 39.0 35.0 41.0 30.0 41.0 62-63 35.904600412975526 39.0 35.0 41.0 30.0 41.0 64-65 35.62145376666163 38.0 35.0 40.5 29.5 41.0 66-67 35.32607306034707 37.5 35.0 40.0 29.0 41.0 68-69 35.01217685784985 37.0 34.5 40.0 29.0 41.0 70-71 34.67748507343876 36.5 34.0 39.5 29.0 41.0 72-73 34.3907434919553 36.0 34.0 39.0 28.5 41.0 74-75 34.11947729234619 36.0 34.0 39.0 28.0 41.0 76-77 33.80365581745873 35.0 34.0 38.5 28.0 40.0 78-79 33.439109710525116 35.0 34.0 37.0 28.0 39.0 80-81 33.127761603836305 35.0 34.0 37.0 27.0 39.0 82-83 32.822506526245384 35.0 34.0 36.0 27.0 39.0 84-85 32.66324453799463 35.0 34.0 36.0 27.0 37.0 86-87 32.43419352567656 35.0 34.0 36.0 27.0 37.0 88-89 32.202690421159836 35.0 33.5 35.0 27.0 37.0 90-91 32.01283528260662 35.0 33.0 35.0 26.0 36.0 92-93 31.825831051247526 35.0 33.0 35.0 26.0 36.0 94-95 31.648362378425432 35.0 33.0 35.0 25.0 36.0 96-97 31.470706098549307 35.0 33.0 35.0 25.0 36.0 98-99 31.294053856425002 35.0 33.0 35.0 24.5 35.0 100 31.165241413077133 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2936163078520053 1101 2 0.3512692827592936 1101 3 0.3031530325202212 1101 4 0.3227728042027138 1101 5 0.2583617140606904 1101 6 0.2955849234482457 1101 7 0.3101474169432379 1101 8 0.3078903503811006 1101 9 0.3697232881650052 1101 10-11 0.3325847449910171 1101 12-13 0.2577956269972148 1101 14-15 0.28090277534321473 1101 16-17 0.3158602048062349 1101 18-19 0.32721237171323025 1101 20-21 0.27055178893639464 1101 22-23 0.33002023324714 1101 24-25 0.21992659695157357 1101 26-27 0.22085464102531205 1101 28-29 0.3026221379886067 1101 30-31 0.32958947772058167 1101 32-33 0.4020101693967675 1101 34-35 0.371887952994463 1101 36-37 0.3596329414472734 1101 38-39 0.39403852004788575 1101 40-41 0.379058794053897 1101 42-43 0.41884632574726766 1101 44-45 0.4103697536716453 1101 46-47 0.3138441722001133 1101 48-49 0.31774931372363113 1101 50-51 0.3004901301091891 1101 52-53 0.2855501509340215 1101 54-55 0.3463753235619791 1101 56-57 0.36774373711221386 1101 58-59 0.3743865806824971 1101 60-61 0.37374860647165065 1101 62-63 0.3084434878950262 1101 64-65 0.230143862343148 1101 66-67 0.2313922133784203 1101 68-69 0.3591076763599119 1101 70-71 0.328473116232594 1101 72-73 0.17411666003735604 1101 74-75 0.16530693209386982 1101 76-77 0.29717174582235373 1101 78-79 0.2795396267107719 1101 80-81 0.23295616086355864 1101 82-83 0.2070740039878629 1101 84-85 0.19326477520329632 1101 86-87 0.10562571974624291 1101 88-89 0.16565986018294154 1101 90-91 0.23949321171258475 1101 92-93 0.2285807396898143 1101 94-95 0.3032093844242567 1101 96-97 0.22375389119286737 1101 98-99 0.26629497024697457 1101 100 0.29237853795503455 1102 1 0.3354427037577494 1102 2 0.28934147426411627 1102 3 0.3466415796826894 1102 4 0.3570431555119171 1102 5 0.18662552325988457 1102 6 0.1731622064670404 1102 7 0.2679451000070543 1102 8 0.3254467233904137 1102 9 0.3656203604129402 1102 10-11 0.35708306259295597 1102 12-13 0.35938533613508383 1102 14-15 0.3435334828304306 1102 16-17 0.3896374969440686 1102 18-19 0.35955002978579387 1102 20-21 0.31314054886569664 1102 22-23 0.38604984396322095 1102 24-25 0.415227934803184 1102 26-27 0.4922815817774193 1102 28-29 0.5030701504057973 1102 30-31 0.5109712154833019 1102 32-33 0.5517404085928703 1102 34-35 0.6049068603237089 1102 36-37 0.4855617039337332 1102 38-39 0.5476955238329211 1102 40-41 0.6209591944305188 1102 42-43 0.5967132497024892 1102 44-45 0.6092531821224583 1102 46-47 0.527087050664754 1102 48-49 0.5493930324148195 1102 50-51 0.4989313172799186 1102 52-53 0.4304351857378137 1102 54-55 0.4263259787454672 1102 56-57 0.48816744060713546 1102 58-59 0.36084449586081035 1102 60-61 0.4533022966993272 1102 62-63 0.40520122480577925 1102 64-65 0.4515870164832094 1102 66-67 0.4373771695225628 1102 68-69 0.39085371316701156 1102 70-71 0.36633783376412765 1102 72-73 0.41507454763537055 1102 74-75 0.4841349958901304 1102 76-77 0.38047109534187484 1102 78-79 0.3749805240492492 1102 80-81 0.3077834173383138 1102 82-83 0.3561198373384755 1102 84-85 0.40745622454625874 1102 86-87 0.3440165672967126 1102 88-89 0.3432964169248578 1102 90-91 0.4147993131436678 1102 92-93 0.5838404615847672 1102 94-95 0.5328019580867647 1102 96-97 0.4176604312941379 1102 98-99 0.5078749290327664 1102 100 0.40548325986115685 1103 1 0.011958182271374795 1103 2 0.054626704522831915 1103 3 0.15071577465322505 1103 4 0.08046749995595803 1103 5 0.04310389259740788 1103 6 0.01651422770498101 1103 7 0.02307960769516626 1103 8 0.14962085040028228 1103 9 0.1251586215979188 1103 10-11 0.10209361357284763 1103 12-13 0.0919656052642992 1103 14-15 0.1733628981439992 1103 16-17 0.2450719976484379 1103 18-19 0.142400587977086 1103 20-21 0.07672953105224423 1103 22-23 0.12133608024917919 1103 24-25 0.12169625925097449 1103 26-27 0.16520166660366442 1103 28-29 0.22631759403520135 1103 30-31 0.22385334816259927 1103 32-33 0.20055691137759624 1103 34-35 0.21578659884276874 1103 36-37 0.20699952914280573 1103 38-39 0.17000551620618864 1103 40-41 0.17810200322781355 1103 42-43 0.1854570047191686 1103 44-45 0.17246030467226348 1103 46-47 0.2180673242241724 1103 48-49 0.21309873837366666 1103 50-51 0.3100101692028616 1103 52-53 0.22267579299677465 1103 54-55 0.20739491499318063 1103 56-57 0.23521927316112823 1103 58-59 0.27689813711026545 1103 60-61 0.31491771186708206 1103 62-63 0.19446512974820251 1103 64-65 0.22999121115532972 1103 66-67 0.22465778559273275 1103 68-69 0.211425370456908 1103 70-71 0.19190378561818733 1103 72-73 0.2118791959119406 1103 74-75 0.19387952342496817 1103 76-77 0.16326827563518265 1103 78-79 0.15846977109477933 1103 80-81 0.12029132018992073 1103 82-83 0.07816374012116256 1103 84-85 0.10691020095353565 1103 86-87 0.08284405023808716 1103 88-89 0.08233710380456927 1103 90-91 0.03252049838528137 1103 92-93 0.07204840710793903 1103 94-95 -0.014598538281539675 1103 96-97 0.04774052275339713 1103 98-99 0.12401969727577722 1103 100 -0.032849885290730185 1104 1 -0.1706266403717187 1104 2 -0.07765733532419006 1104 3 -0.0016057487011416072 1104 4 -0.055987327562775135 1104 5 -0.025392761802706332 1104 6 -0.18638782820411848 1104 7 0.019735635976829258 1104 8 -0.06712126975949673 1104 9 -0.10355560410655329 1104 10-11 -0.141557029007906 1104 12-13 -0.12294644822031131 1104 14-15 -0.04766279768541182 1104 16-17 -0.1213987060391375 1104 18-19 -0.1715541292699143 1104 20-21 -0.16450934297421327 1104 22-23 -0.180060833148886 1104 24-25 -0.04318514017276698 1104 26-27 -0.012355061170808312 1104 28-29 -0.10144136765210732 1104 30-31 -0.13250033131640748 1104 32-33 -0.028326052276547387 1104 34-35 -0.05882272099823638 1104 36-37 -0.08266980072183117 1104 38-39 -0.0587132979863938 1104 40-41 0.025128026468060227 1104 42-43 -0.05642233343731107 1104 44-45 -0.09641518122641912 1104 46-47 -0.07961839039140983 1104 48-49 -0.0679101902321193 1104 50-51 -0.10573986062230745 1104 52-53 -0.10521209040471291 1104 54-55 -0.10042514167317051 1104 56-57 -0.12123239274128395 1104 58-59 -0.14579871569014813 1104 60-61 -0.10652307931852079 1104 62-63 -0.050101434264611555 1104 64-65 -0.12596075359088132 1104 66-67 -0.11679337261568179 1104 68-69 -0.09918744729218787 1104 70-71 -0.12502116359021187 1104 72-73 -0.17059408206547033 1104 74-75 -0.09775249522264318 1104 76-77 -0.13568178947694776 1104 78-79 -0.13181841260104932 1104 80-81 -0.14912249897450636 1104 82-83 -0.13848320591436192 1104 84-85 -0.1783192486143932 1104 86-87 -0.2506033045577212 1104 88-89 -0.18956944852683932 1104 90-91 -0.20956117729505053 1104 92-93 -0.21911129329635415 1104 94-95 -0.2405220751236925 1104 96-97 -0.3513017857712164 1104 98-99 -0.2651791201376241 1104 100 -0.26742139006912424 1105 1 0.012496450720799857 1105 2 0.03231220304810023 1105 3 0.03836155936228991 1105 4 0.027054120358357636 1105 5 0.08224390817283478 1105 6 0.11530102406507581 1105 7 0.03416388532304637 1105 8 0.10950590233598945 1105 9 0.06883571456067017 1105 10-11 0.13717018688483051 1105 12-13 0.13808549142036952 1105 14-15 0.2178806060894729 1105 16-17 0.13554024127819275 1105 18-19 0.21406164976078657 1105 20-21 0.2163047717607327 1105 22-23 0.2621376215822053 1105 24-25 0.2812049050227614 1105 26-27 0.28189389098848494 1105 28-29 0.32080044520764517 1105 30-31 0.2512971104153294 1105 32-33 0.22856537097251106 1105 34-35 0.16919848080311795 1105 36-37 0.14763876770878426 1105 38-39 0.14218220568783835 1105 40-41 0.13972343341853133 1105 42-43 0.24043365332413202 1105 44-45 0.20239102573468415 1105 46-47 0.0909701915828478 1105 48-49 0.12112105258149342 1105 50-51 0.14816476882862872 1105 52-53 0.18139400931666927 1105 54-55 0.17522904373829817 1105 56-57 0.12011894908417275 1105 58-59 0.1532975469824578 1105 60-61 0.09789700571055704 1105 62-63 0.07346793238546212 1105 64-65 0.07494919508890519 1105 66-67 0.1578411398538293 1105 68-69 0.08730956374726162 1105 70-71 0.0796752543950916 1105 72-73 0.0908489624332347 1105 74-75 0.13266903315138734 1105 76-77 0.027062865582095696 1105 78-79 0.09698368620047404 1105 80-81 0.11422295275548322 1105 82-83 0.054651862976761834 1105 84-85 0.06486241986464591 1105 86-87 0.04827931648275552 1105 88-89 0.04485623538639061 1105 90-91 -0.004211845058762265 1105 92-93 0.11231693640465679 1105 94-95 0.15726737678657088 1105 96-97 0.024513490342176425 1105 98-99 -0.061621690840148347 1105 100 -0.0036697447829929786 1106 1 -0.18197636505302128 1106 2 -0.1720236807508151 1106 3 -0.12187770879772941 1106 4 -0.20774749389028102 1106 5 -0.06073596024574357 1106 6 0.024489291406453617 1106 7 -0.18715667020358495 1106 8 -0.013658100318238553 1106 9 -0.05347253248628192 1106 10-11 -0.0806673879314701 1106 12-13 -0.07790030026711037 1106 14-15 -0.1426686173053966 1106 16-17 -0.18798065854467438 1106 18-19 -0.15176030211528513 1106 20-21 -0.08625925482142094 1106 22-23 -0.040825203729554005 1106 24-25 -0.053554226083427636 1106 26-27 -0.06003350881056235 1106 28-29 -0.03195295538235854 1106 30-31 -0.08146775009927154 1106 32-33 -0.055232267474345065 1106 34-35 -0.05785743742542593 1106 36-37 -0.01395752390614291 1106 38-39 -0.04295802745432553 1106 40-41 -0.015477677457475636 1106 42-43 -0.08393884795258089 1106 44-45 -0.08094356307613282 1106 46-47 -0.039343939960922114 1106 48-49 -0.03285832176977266 1106 50-51 -0.16830210152079417 1106 52-53 0.03956858013723519 1106 54-55 0.008430228835194953 1106 56-57 -0.08320706001507716 1106 58-59 -0.062010286864698116 1106 60-61 -0.14856351709241977 1106 62-63 -0.18120313620681827 1106 64-65 -0.09762887807071507 1106 66-67 -0.10372623885319854 1106 68-69 -0.08831959663740463 1106 70-71 -0.08553736625537312 1106 72-73 -0.16541910121061676 1106 74-75 -0.1565835626406198 1106 76-77 -0.08091161351475762 1106 78-79 0.023197474444998534 1106 80-81 -0.06456156410538938 1106 82-83 -0.09573084533187881 1106 84-85 -0.03989646753121434 1106 86-87 -0.03858333945031944 1106 88-89 0.05237137589094232 1106 90-91 0.05849897822797345 1106 92-93 -0.0019657631525653585 1106 94-95 0.015837838429334283 1106 96-97 0.09064802841693265 1106 98-99 0.0412399946826838 1106 100 0.022112870029502574 1107 1 0.07279027619156153 1107 2 0.11721584330505763 1107 3 0.17428177835167347 1107 4 0.15880868447676733 1107 5 0.23686431921943552 1107 6 0.26846665686050386 1107 7 0.22340647345965436 1107 8 0.2755846237205688 1107 9 0.2068343458770343 1107 10-11 0.18052655236751036 1107 12-13 0.16773625633792477 1107 14-15 0.26532867337572696 1107 16-17 0.22039476345307918 1107 18-19 0.2025260750240392 1107 20-21 0.18935274191459683 1107 22-23 0.21708088743795884 1107 24-25 0.2009238833666558 1107 26-27 0.23677424154810467 1107 28-29 0.19574249690855794 1107 30-31 0.24013626801262689 1107 32-33 0.2594391209449114 1107 34-35 0.22410145879379684 1107 36-37 0.29369883525994567 1107 38-39 0.2169476530487131 1107 40-41 0.2282066228929196 1107 42-43 0.2580913278864827 1107 44-45 0.21234047274234058 1107 46-47 0.23745631710437465 1107 48-49 0.28308985983992585 1107 50-51 0.28137576686390275 1107 52-53 0.1615431296063008 1107 54-55 0.17573346988457672 1107 56-57 0.22809594636888875 1107 58-59 0.19143249477379243 1107 60-61 0.28598669863976767 1107 62-63 0.2760325585361443 1107 64-65 0.3005540649535803 1107 66-67 0.20196959743567078 1107 68-69 0.25631936528813526 1107 70-71 0.3515576359046193 1107 72-73 0.39903402170606483 1107 74-75 0.3737830754039777 1107 76-77 0.3074835150445594 1107 78-79 0.27051842206661547 1107 80-81 0.17674861557365062 1107 82-83 0.17280678913406433 1107 84-85 0.24525161167903065 1107 86-87 0.23708425698917068 1107 88-89 0.21180252559148016 1107 90-91 0.22092171197339638 1107 92-93 0.25028355818438897 1107 94-95 0.2952047484724787 1107 96-97 0.3039214242921524 1107 98-99 0.4042655252131837 1107 100 0.3696872829659661 1108 1 0.07120706558773193 1108 2 0.15854237391730308 1108 3 0.12548628077119872 1108 4 0.1123117351815921 1108 5 0.11246394051124753 1108 6 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74-75 -0.3786699264152986 2214 76-77 -0.3450180010257853 2214 78-79 -0.23770292420385886 2214 80-81 -0.19258067490542885 2214 82-83 -0.26343517569310393 2214 84-85 -0.3236640948097502 2214 86-87 -0.21524442706176927 2214 88-89 -0.20030663147340988 2214 90-91 -0.2952341400581382 2214 92-93 -0.2249353623735253 2214 94-95 -0.23353448742806648 2214 96-97 -0.2696158710383507 2214 98-99 -0.22247431711549837 2214 100 -0.19566747749491853 2215 1 -0.044752912960234426 2215 2 -0.14636932797603208 2215 3 -0.16358970156917962 2215 4 -0.07557887859559997 2215 5 -0.0680283549522187 2215 6 -0.10860813983239836 2215 7 -0.11445339582392222 2215 8 -0.16268839073951114 2215 9 -0.09925398204606495 2215 10-11 -0.13735999719297354 2215 12-13 -0.15241835864851083 2215 14-15 -0.20823680819964352 2215 16-17 -0.15659585611955862 2215 18-19 -0.09374037942598079 2215 20-21 -0.23765831721740938 2215 22-23 -0.2353423493174489 2215 24-25 -0.15504035587052556 2215 26-27 -0.1946588981133459 2215 28-29 -0.21008011674264537 2215 30-31 -0.2441991681361273 2215 32-33 -0.22831842959763549 2215 34-35 -0.27941329880995625 2215 36-37 -0.2484241939553158 2215 38-39 -0.254229060850669 2215 40-41 -0.19389736971497484 2215 42-43 -0.22129011541248644 2215 44-45 -0.22750360607453501 2215 46-47 -0.19259092367605035 2215 48-49 -0.21811571133086005 2215 50-51 -0.20726685288288138 2215 52-53 -0.2515848728319341 2215 54-55 -0.13858184115956362 2215 56-57 -0.18606213729543697 2215 58-59 -0.16092821386209266 2215 60-61 -0.1379428247919563 2215 62-63 -0.12748086868209185 2215 64-65 -0.19962444859458373 2215 66-67 -0.2703894663599371 2215 68-69 -0.16771102555494366 2215 70-71 -0.12978673075930658 2215 72-73 -0.19895576577089713 2215 74-75 -0.20609532735934977 2215 76-77 -0.1960931659580183 2215 78-79 -0.19974129337602164 2215 80-81 -0.22541386561953658 2215 82-83 -0.17227598503419728 2215 84-85 -0.18754142387370365 2215 86-87 -0.25005768788933125 2215 88-89 -0.22230227008549264 2215 90-91 -0.16414495243564886 2215 92-93 -0.22302165160531473 2215 94-95 -0.26887641471986257 2215 96-97 -0.37612968670070046 2215 98-99 -0.4269595905303518 2215 100 -0.45758924854965954 2216 1 0.1474597924998733 2216 2 0.0770955286359225 2216 3 -0.06401664210086722 2216 4 0.005786505932093178 2216 5 -0.06459954105907428 2216 6 -0.06475059174051978 2216 7 -0.10014075791948329 2216 8 -0.07951109847753912 2216 9 -0.1599088094968124 2216 10-11 -0.10626376044108099 2216 12-13 -0.24145145267423374 2216 14-15 -0.25502654590515306 2216 16-17 -0.2743747447452094 2216 18-19 -0.330979556675679 2216 20-21 -0.23931680778795084 2216 22-23 -0.1510240372580256 2216 24-25 -0.1523671187944302 2216 26-27 -0.2345556124236623 2216 28-29 -0.2233820254345673 2216 30-31 -0.3213772304154574 2216 32-33 -0.37744099851104096 2216 34-35 -0.2689991723572973 2216 36-37 -0.2984448124403798 2216 38-39 -0.21308557421816232 2216 40-41 -0.25859001096788603 2216 42-43 -0.2268431001417781 2216 44-45 -0.13112570605041185 2216 46-47 -0.11290183609884963 2216 48-49 -0.0960764291538041 2216 50-51 -0.1558399646845885 2216 52-53 -0.1691454595825448 2216 54-55 -0.09508867240350583 2216 56-57 -0.17396889092962198 2216 58-59 -0.049925195680700085 2216 60-61 -0.06822693861294482 2216 62-63 -0.05368330420399303 2216 64-65 -0.024793592917681906 2216 66-67 -0.08198482897058312 2216 68-69 -0.10440304749327112 2216 70-71 -0.15112463700263845 2216 72-73 -0.07789974320804305 2216 74-75 -0.08688888822491947 2216 76-77 -0.008760277205112743 2216 78-79 0.18611818258222002 2216 80-81 0.1076020291803701 2216 82-83 0.04871725918182079 2216 84-85 0.0608636565036349 2216 86-87 -0.0133112262016013 2216 88-89 0.06820968722435339 2216 90-91 -0.018461186360234194 2216 92-93 -0.13147460074356232 2216 94-95 -0.15064780642526188 2216 96-97 -0.03587484700263488 2216 98-99 -0.09084484274361415 2216 100 -0.05634853473965862 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19028.0 3 2953.0 4 1754.0 5 1579.0 6 1240.0 7 1442.0 8 1656.0 9 1735.0 10 1836.0 11 1975.0 12 2246.0 13 2319.0 14 2570.0 15 2686.0 16 2581.0 17 2653.0 18 2497.0 19 2445.0 20 2468.0 21 2619.0 22 3084.0 23 4783.0 24 7474.0 25 10075.0 26 10406.0 27 8677.0 28 8452.0 29 10555.0 30 12984.0 31 15430.0 32 19764.0 33 26935.0 34 39424.0 35 63095.0 36 120171.0 37 251750.0 38 397312.0 39 178930.0 40 370.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.45419469307134 19.316461310951176 12.621109697502986 23.608234298474503 2 16.129569489549507 19.96499420306565 40.11214345651018 23.793292850874664 3 18.305022319194507 25.765758552355024 29.46526012370639 26.46395900474408 4 12.439476884914201 16.74917907956022 38.49466291617524 32.31668111935034 5 12.793502882083201 37.8243494594943 34.86171685524248 14.520430803180021 6 30.54320942183263 38.00384169156133 18.13009386442304 13.322855022183006 7 27.86954025703458 32.19818133340593 22.29135656962681 17.64092183993268 8 25.37287858746513 36.13455336239283 20.384696684847533 18.10787136529451 9 25.25818017605414 17.340147677939747 20.878285001449235 36.52338714455688 10-11 23.881783093923936 26.978405313619575 28.49060584502957 20.649205747426922 12-13 24.723949221549216 24.471057804324413 28.373079894792046 22.431913079334322 14-15 22.12024514531333 26.465293298857024 25.38536646293937 26.02909509289028 16-17 21.907100937557548 29.644352636930638 25.72206342724922 22.726482998262597 18-19 21.76242735182334 28.453305406969903 27.772800458607254 22.011466782599506 20-21 23.12905399157166 28.005513208361403 27.016082059214547 21.849350740852387 22-23 22.796783938266472 27.839454723490594 27.08451930013347 22.279242038109466 24-25 22.425206609585146 27.749176524090974 27.284074768570044 22.541542097753837 26-27 22.29280417684329 27.58159348236943 27.4349940331382 22.69060830764907 28-29 22.36813925625012 27.806175244818903 27.266759031268528 22.55892646766245 30-31 22.64826900396569 28.313649003213065 26.83894558926085 22.199136403560395 32-33 22.11121661892127 27.968407972547332 27.43657655089809 22.48379885763331 34-35 22.202339991745536 27.40941609091597 27.616658320133 22.771585597205494 36-37 22.719550575111104 28.07232301333079 26.908450574550685 22.29967583700742 38-39 22.551088256216076 28.122529351553734 27.1123131013871 22.21406929084308 40-41 22.166924872198273 27.754212353033015 27.531114767354815 22.5477480074139 42-43 22.107261915866616 27.76172630488333 27.484449732511777 22.64656204673828 44-45 22.319763573429714 27.96399955481857 27.39367089722474 22.322565974526974 46-47 22.248841564822325 28.323790138868095 27.15862895083046 22.26873934547912 48-49 22.09864146580123 27.9108072572599 27.515682478063745 22.47486879887512 50-51 22.12451349716316 27.709872349182618 27.565578040039668 22.600036113614554 52-53 22.210064243772408 27.71082214892736 27.459188028541753 22.619925578758476 54-55 22.325803370026257 28.321080560811573 27.187702438997047 22.165413630165123 56-57 21.981326102179153 28.004160609454015 27.334227218441203 22.68028606992562 58-59 21.91362856206013 27.43036024064887 27.763620574862063 22.89239062242893 60-61 22.44453812699751 27.992707173561342 27.127825039424742 22.434929660016405 62-63 22.133743617830078 28.03386528592001 27.181548087320778 22.650843008929133 64-65 22.089336287401945 27.60379342124543 27.559154102933864 22.74771618841876 66-67 22.007180684535104 27.715416532549288 27.29456820609397 22.982834576821634 68-69 22.5216918892972 27.89168808198179 27.173542721001446 22.41307730771956 70-71 22.52365213269535 27.564582973026642 27.348118718653318 22.56364617562469 72-73 22.08361991037178 27.71817153307172 27.67405401553481 22.524154541021684 74-75 22.18385641607056 27.72698812274709 27.479658783194093 22.60949667798825 76-77 22.29051970138068 27.73582246818923 27.26554668408933 22.70811114634076 78-79 22.3700361025283 27.877933580635784 27.292278350573245 22.459751966262672 80-81 22.150693898140197 27.676537175044892 27.472321140823393 22.700447785991525 82-83 22.035560067866147 27.736084277008544 27.54504059077818 22.68331506434713 84-85 22.140246367934537 27.71214905380141 27.39295965795131 22.754644920312746 86-87 22.354094630262836 27.79297072824876 27.332618323859258 22.520316317629145 88-89 21.97440627457009 27.65677217693186 27.4486820424379 22.920139506060156 90-91 21.95382352058126 27.589435654166795 27.5160576855716 22.94068313968035 92-93 22.267159801331687 27.81632588520648 27.22345941786998 22.693054895591853 94-95 22.048898629917634 27.489556749832367 27.453688413846937 23.00785620640306 96-97 22.054446577223388 27.45747174290295 27.590856049430656 22.897225630443007 98-99 21.967455532475412 27.603471335144608 27.51896572498888 22.9101074073911 100 22.229934805983152 27.55273846094724 27.229610557219498 22.98771617585011 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1340.0 1 858.0 2 361.0 3 289.5 4 192.5 5 149.0 6 137.5 7 136.0 8 139.0 9 129.5 10 132.5 11 134.5 12 134.0 13 142.0 14 165.0 15 190.0 16 225.0 17 317.5 18 445.5 19 586.5 20 846.5 21 1233.5 22 1776.0 23 2702.5 24 3916.5 25 5416.5 26 7430.5 27 9963.0 28 12925.0 29 16229.5 30 19426.0 31 22522.0 32 25500.0 33 28478.5 34 31435.5 35 33832.5 36 36108.5 37 38235.0 38 40090.0 39 40759.0 40 41580.0 41 42646.5 42 42701.5 43 42743.0 44 42119.5 45 41470.5 46 41502.0 47 41110.0 48 40749.5 49 40434.5 50 39601.0 51 38338.5 52 35922.5 53 34002.5 54 33011.5 55 31637.5 56 29650.0 57 27299.0 58 25317.0 59 23810.5 60 21678.0 61 18966.5 62 16523.5 63 14197.5 64 11916.5 65 9826.5 66 7966.0 67 6203.0 68 4886.0 69 3859.0 70 2923.0 71 2302.5 72 1866.0 73 1554.5 74 1292.5 75 974.5 76 741.0 77 608.5 78 465.0 79 333.0 80 269.0 81 176.0 82 111.5 83 84.0 84 57.5 85 39.0 86 26.0 87 21.0 88 19.5 89 15.5 90 8.0 91 6.0 92 7.0 93 7.0 94 6.0 95 2.5 96 1.0 97 1.0 98 1.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07856295396706917 2 0.08136305925102784 3 0.082243092340272 4 0.0840031585187603 5 0.08240309835649821 6 0.08192308030781957 7 0.08128305624291474 8 0.08168307128348026 9 0.08152306526725404 10-11 0.08092304270640575 12-13 0.07984300209687883 14-15 0.0783629464467864 16-17 0.07704289681292016 18-19 0.07632286973990222 20-21 0.07620286522773256 22-23 0.07776292388593811 24-25 0.07832294494272984 26-27 0.07772292238188157 28-29 0.07340275994377389 30-31 0.079202978031974 32-33 0.07736290884537259 34-35 0.07452280205735735 36-37 0.0712026772206635 38-39 0.07308274791132147 40-41 0.0758428516912236 42-43 0.0815630667713106 44-45 0.08192308030781957 46-47 0.0856032186810224 48-49 0.08856332998120729 50-51 0.08932335855828179 52-53 0.08928335705422524 54-55 0.08960336908667767 56-57 0.0878033014041328 58-59 0.08636324725809691 60-61 0.08436317205526928 62-63 0.08508319912828723 64-65 0.08432317055121273 66-67 0.08376314949442099 68-69 0.08332313294979891 70-71 0.08136305925102784 72-73 0.08128305624291474 74-75 0.08088304120234921 76-77 0.08040302315367058 78-79 0.08080303819423611 80-81 0.08244309986055476 82-83 0.08192308030781957 84-85 0.08064303217800989 86-87 0.0814830637631975 88-89 0.07800293291027742 90-91 0.0752428291303753 92-93 0.07492281709792288 94-95 0.0752428291303753 96-97 0.07444279904924425 98-99 0.07388277799245252 100 0.07296274339915182 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1249953.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.1948014450757 #Duplication Level Percentage of deduplicated Percentage of total 1 85.5647197391034 65.19586831224532 2 9.737665950989749 14.839190473482763 3 2.2257569170975904 5.087733190897642 4 0.8468439594251645 2.581004293734486 5 0.4615710213378375 1.7584656161818664 6 0.2737248190941758 1.2513844944881996 7 0.18161062728161817 0.9686449980226979 8 0.1406225820561289 0.8571767774768485 9 0.09533235677058183 0.6537446995883041 >10 0.4578391308716315 5.8212253391519875 >50 0.011690109942869436 0.5972309368334144 >100 0.0024129674532849255 0.2616332213620146 >500 2.0981857597875253E-4 0.1266976465343024 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.0001504056552525E-5 0.0 24-25 0.0 0.0 0.0 1.600060162262101E-4 0.0 26-27 0.0 0.0 0.0 3.200120324524202E-4 0.0 28-29 0.0 0.0 0.0 0.0010400391054703657 0.0 30-31 0.0 0.0 0.0 0.00460017296650354 0.0 32-33 0.0 0.0 0.0 0.014200533940076147 0.0 34-35 0.0 0.0 0.0 0.02548095808402396 0.0 36-37 0.0 0.0 0.0 0.04396165295815123 0.0 38-39 0.0 4.0001504056552525E-5 0.0 0.07660288026829809 0.0 40-41 8.000300811310505E-5 8.000300811310505E-5 0.0 0.12396466107125628 0.0 42-43 8.000300811310505E-5 8.000300811310505E-5 0.0 0.17480657272713454 0.0 44-45 8.000300811310505E-5 8.000300811310505E-5 0.0 0.23192872051989155 0.0 46-47 8.000300811310505E-5 1.600060162262101E-4 0.0 0.3024913736756502 0.0 48-49 8.000300811310505E-5 1.600060162262101E-4 0.0 0.3800942915453621 0.0 50-51 1.600060162262101E-4 1.600060162262101E-4 0.0 0.47149772831458464 0.0 52-53 1.600060162262101E-4 1.600060162262101E-4 0.0 0.5661012854083314 0.0 54-55 1.600060162262101E-4 1.600060162262101E-4 0.0 0.661064856038587 0.0 56-57 1.600060162262101E-4 2.0000752028276263E-4 0.0 0.769148919999392 0.0 58-59 1.600060162262101E-4 2.4000902433931516E-4 0.0 0.8977537555412083 0.0 60-61 1.600060162262101E-4 2.4000902433931516E-4 0.0 1.0358389475444276 0.0 62-63 1.600060162262101E-4 2.4000902433931516E-4 0.0 1.1784443095060375 8.000300811310505E-5 64-65 1.600060162262101E-4 2.4000902433931516E-4 0.0 1.3282499421978267 8.000300811310505E-5 66-67 1.600060162262101E-4 2.4000902433931516E-4 0.0 1.498616347974684 8.000300811310505E-5 68-69 2.4000902433931516E-4 3.200120324524202E-4 0.0 1.6886234922433083 8.000300811310505E-5 70-71 2.8001052839586767E-4 3.200120324524202E-4 0.0 1.895871284760307 8.000300811310505E-5 72-73 3.200120324524202E-4 3.200120324524202E-4 0.0 2.1272399842234067 8.000300811310505E-5 74-75 3.200120324524202E-4 3.200120324524202E-4 0.0 2.355648572386322 8.000300811310505E-5 76-77 3.200120324524202E-4 3.200120324524202E-4 0.0 2.587577292906213 8.000300811310505E-5 78-79 3.200120324524202E-4 3.200120324524202E-4 0.0 2.8500671625253107 8.000300811310505E-5 80-81 3.200120324524202E-4 3.200120324524202E-4 0.0 3.1479583632344577 8.000300811310505E-5 82-83 3.200120324524202E-4 3.200120324524202E-4 0.0 3.456769974551043 8.000300811310505E-5 84-85 3.200120324524202E-4 3.200120324524202E-4 0.0 3.7657015903797983 8.000300811310505E-5 86-87 3.200120324524202E-4 4.0001504056552526E-4 0.0 4.08743368750665 8.000300811310505E-5 88 3.200120324524202E-4 4.0001504056552526E-4 0.0 4.343443313468587 8.000300811310505E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGATCG 60 8.8245666E-5 39.166634 9 GGTATCA 1120 0.0 34.830326 1 GTATCAA 1835 0.0 32.784714 1 TCAACGC 1895 0.0 30.75459 4 CAACGCA 1990 0.0 29.522585 5 ATCAACG 1980 0.0 29.434319 3 AACGCAG 2075 0.0 28.086723 6 GTACATG 4135 0.0 27.96128 1 TAGACCG 70 0.008836346 26.857119 5 GTACACG 160 6.917071E-8 26.437477 1 GAGTACG 125 8.024694E-6 26.319977 1 TACATGG 4280 0.0 26.245306 2 TATCAAC 2305 0.0 25.895855 2 ACATGGG 4240 0.0 24.719318 3 ACGCAGA 2565 0.0 22.72123 7 TACACGG 190 3.6403253E-7 22.263138 2 TCTGTCG 320 0.0 22.03123 8 CGCAGAG 2680 0.0 21.395504 8 TACCTGG 985 0.0 20.040592 2 CATGGGG 2810 0.0 19.56938 4 >>END_MODULE