FastQCFastQC Report
Wed 25 May 2016
SRR1032918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249953
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32030.25624963498627545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25700.20560773085068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13300.10640400079042972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17850.050.2721371
GTATCAA35600.045.6623541
TCAACGC42650.037.9350744
ATCAACG42900.037.4947433
CAACGCA43500.037.3004465
TATCAAC44750.036.1548842
AACGCAG45150.035.624816
TACCTGG9550.031.5194172
ACGCAGA52250.030.782697
CGCAGAG54000.029.6109248
GTACATG43350.028.1784081
GTACCTG11600.027.5412181
TACATGG43650.027.1530022
GCAGAGT59600.026.4055989
ACATGGG45450.025.2497733
ACCTGGG11750.023.2162323
CATGGGG29400.023.0365124
AGAGTAC53550.022.58994910-11
ATAACGC1252.4121525E-422.575783
AAGACCG1252.4128065E-422.5748775
Produced by FastQC (version 0.11.5)