FastQCFastQC Report
Wed 25 May 2016
SRR1032917_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032917_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905719
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG67350.028.336061
GTATCAA36750.028.1394981
TCAACGC38000.026.2242624
TACATGG69300.026.1821352
ATCAACG38650.025.7832343
CAACGCA38800.025.6822055
GGTATCA28500.024.90481
AACGCAG40150.024.7009536
ACATGGG69550.024.6687263
TATCAAC42600.023.9442422
ACGCAGA46900.021.045147
CTTACGC1150.00395677320.4372833
CGCAGAG49200.019.7747338
CATGGGG44350.019.7137994
GAGTACA47300.019.6768261
ACACCGT3204.5656634E-1019.0945846
TATACCG1250.00641009718.8013135
GACTCGT2001.4080282E-518.8003257
AGTACAT46050.018.1694342
GTATAGA8200.017.7704681
Produced by FastQC (version 0.11.5)