##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032917_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1905719
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39726370991736	33.0	31.0	34.0	30.0	34.0
2	31.416067636414393	33.0	31.0	34.0	28.0	34.0
3	31.601060282234684	34.0	31.0	34.0	30.0	34.0
4	34.91560455660042	37.0	35.0	37.0	32.0	37.0
5	35.002437400267304	37.0	35.0	37.0	33.0	37.0
6	35.041377034074806	37.0	35.0	37.0	33.0	37.0
7	34.98207133370659	37.0	35.0	37.0	33.0	37.0
8	35.00615200876939	37.0	36.0	37.0	33.0	37.0
9	36.62217514754274	39.0	38.0	39.0	33.0	39.0
10-11	36.61296838621014	39.0	38.0	39.0	33.0	39.0
12-13	36.5472097407855	39.0	38.0	39.0	33.0	39.0
14-15	37.99546863939542	41.0	39.0	41.0	33.0	41.0
16-17	38.01388557284679	41.0	39.0	41.0	33.0	41.0
18-19	38.01351432189111	41.0	39.0	41.0	33.0	41.0
20-21	38.05601219277344	41.0	39.0	41.0	33.0	41.0
22-23	38.04695025866877	41.0	39.0	41.0	33.0	41.0
24-25	38.08596283082658	41.0	39.0	41.0	33.0	41.0
26-27	38.07421372196006	41.0	39.0	41.0	33.0	41.0
28-29	38.052108941559595	41.0	39.0	41.0	33.0	41.0
30-31	38.00256386172358	41.0	39.0	41.0	33.0	41.0
32-33	37.95754253381532	41.0	39.0	41.0	33.0	41.0
34-35	37.87783219876593	41.0	38.0	41.0	33.0	41.0
36-37	37.778049124766035	41.0	38.0	41.0	32.0	41.0
38-39	37.67554817892879	40.0	38.0	41.0	32.0	41.0
40-41	37.58531294487803	40.0	38.0	41.0	32.0	41.0
42-43	37.45029146479622	40.0	38.0	41.0	31.5	41.0
44-45	37.28910610640918	40.0	38.0	41.0	31.0	41.0
46-47	37.15077380243362	40.0	37.5	41.0	31.0	41.0
48-49	37.00611501485791	40.0	37.0	41.0	30.5	41.0
50-51	36.8545887405226	40.0	37.0	41.0	30.0	41.0
52-53	36.69217549911608	40.0	36.0	41.0	30.0	41.0
54-55	36.51676873662906	40.0	36.0	41.0	30.0	41.0
56-57	36.32822861082877	39.5	35.0	41.0	30.0	41.0
58-59	36.11140572141014	39.0	35.0	41.0	30.0	41.0
60-61	35.86000113343048	39.0	35.0	41.0	29.0	41.0
62-63	35.598189712124395	38.5	35.0	40.5	29.0	41.0
64-65	35.31746495679583	38.0	35.0	40.0	29.0	41.0
66-67	35.01591446587876	37.0	34.5	40.0	28.5	41.0
68-69	34.697985642164454	37.0	34.0	40.0	28.0	41.0
70-71	34.36021758716789	36.0	34.0	39.0	28.0	41.0
72-73	34.071593713448834	36.0	34.0	39.0	28.0	41.0
74-75	33.80273691976625	35.5	34.0	39.0	28.0	40.5
76-77	33.48669242422414	35.0	34.0	37.5	27.0	40.0
78-79	33.12911373607547	35.0	34.0	37.0	27.0	39.0
80-81	32.82111580983346	35.0	33.5	37.0	26.5	39.0
82-83	32.51820179155479	35.0	33.0	36.0	26.0	38.5
84-85	32.36631659756763	35.0	33.0	36.0	26.0	37.0
86-87	32.147584192632806	35.0	33.0	35.5	26.0	37.0
88-89	31.92898638256742	35.0	33.0	35.0	26.0	36.5
90-91	31.73521516026235	35.0	33.0	35.0	25.0	36.0
92-93	31.553157364753147	35.0	33.0	35.0	25.0	36.0
94-95	31.375492137088415	35.0	33.0	35.0	25.0	36.0
96-97	31.2008651327924	35.0	33.0	35.0	24.0	35.5
98-99	31.024561595912097	35.0	33.0	35.0	23.5	35.0
100	30.89572334641151	35.0	33.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3646914092191267
1101	2	0.4059783047868315
1101	3	0.38494850089372434
1101	4	0.40300745234528534
1101	5	0.3883301032877
1101	6	0.40104264545376367
1101	7	0.3880055164364009
1101	8	0.24052011378748972
1101	9	0.30316624128405323
1101	10-11	0.3301120533866424
1101	12-13	0.19191427744191003
1101	14-15	0.1794965542172804
1101	16-17	0.1699019455776707
1101	18-19	0.15837980244647554
1101	20-21	0.1451209857682585
1101	22-23	0.15405071814373628
1101	24-25	0.1128491230111095
1101	26-27	0.18977922693327542
1101	28-29	0.18365258113971805
1101	30-31	0.19101370613027058
1101	32-33	0.17960231913865954
1101	34-35	0.21366306822570635
1101	36-37	0.1571405397004213
1101	38-39	0.10493896176413386
1101	40-41	0.15746296376489255
1101	42-43	0.20270535307828652
1101	44-45	0.13874024209480496
1101	46-47	0.1269205117207548
1101	48-49	0.1928904356623775
1101	50-51	0.24655476792406716
1101	52-53	0.2348901408159918
1101	54-55	0.1915257365921832
1101	56-57	0.24379156219730191
1101	58-59	0.21238239045128182
1101	60-61	0.2788602319052984
1101	62-63	0.16232770705217092
1101	64-65	0.21253745068444374
1101	66-67	0.17774538495479675
1101	68-69	0.12379673426298154
1101	70-71	0.13942726256094318
1101	72-73	0.09440000237160717
1101	74-75	0.1275682421165527
1101	76-77	0.12505378266955347
1101	78-79	0.08701317573484602
1101	80-81	0.11213411448908772
1101	82-83	0.1100729429879479
1101	84-85	0.008269001060504877
1101	86-87	-0.030364432447512968
1101	88-89	0.03276566447739171
1101	90-91	-0.010168130257305563
1101	92-93	0.09926710529887117
1101	94-95	0.04196221726211391
1101	96-97	-0.016458145340834562
1101	98-99	0.07581734313924215
1101	100	0.12175233285020681
1102	1	0.0295942355473926
1102	2	-0.012981621365984353
1102	3	0.153083387723985
1102	4	0.1436628371989599
1102	5	0.10971899291838128
1102	6	0.1505977909449996
1102	7	0.08606747294169281
1102	8	0.1803505928417195
1102	9	0.1290249506852419
1102	10-11	0.08742912004404957
1102	12-13	0.05923080110531487
1102	14-15	0.16590612657835635
1102	16-17	0.1616210645743621
1102	18-19	0.1285763306674852
1102	20-21	0.09983306097559819
1102	22-23	0.21175384463197133
1102	24-25	0.24553883042636215
1102	26-27	0.23821473660435544
1102	28-29	0.2511471801297489
1102	30-31	0.25750443384013977
1102	32-33	0.26621475761788105
1102	34-35	0.2898749462639145
1102	36-37	0.26196664364726985
1102	38-39	0.21992728739317613
1102	40-41	0.19761714735562208
1102	42-43	0.219998821750643
1102	44-45	0.22900409266998167
1102	46-47	0.258355546939697
1102	48-49	0.35548843050126777
1102	50-51	0.291389826821117
1102	52-53	0.270813867072242
1102	54-55	0.2616850118643299
1102	56-57	0.31035238954211763
1102	58-59	0.3008318844431912
1102	60-61	0.4277131348313219
1102	62-63	0.34120662028084325
1102	64-65	0.3447843064376386
1102	66-67	0.4002371755086571
1102	68-69	0.34753474054924993
1102	70-71	0.2804410234530863
1102	72-73	0.2207464717089067
1102	74-75	0.2452601882693557
1102	76-77	0.2991648080291469
1102	78-79	0.27611995951018997
1102	80-81	0.2540736356395925
1102	82-83	0.23932042451947666
1102	84-85	0.2220808990381684
1102	86-87	0.2414628135670469
1102	88-89	0.27371391002817447
1102	90-91	0.2767111764194752
1102	92-93	0.23313426404272164
1102	94-95	0.25887096133480014
1102	96-97	0.23994428798440026
1102	98-99	0.23796247208251486
1102	100	0.2364998717966742
1103	1	0.15974046579637857
1103	2	0.21519799254246053
1103	3	0.2777642976225856
1103	4	0.2874959809202977
1103	5	0.30767675937159567
1103	6	0.30068599679449903
1103	7	0.35261883617084777
1103	8	0.3514055040768511
1103	9	0.42150920350160703
1103	10-11	0.3284637301360718
1103	12-13	0.43289736507141185
1103	14-15	0.3716399762025304
1103	16-17	0.48492978390963515
1103	18-19	0.5103954991456305
1103	20-21	0.44436303233038643
1103	22-23	0.4390208885880895
1103	24-25	0.3933312802559925
1103	26-27	0.45883732395886767
1103	28-29	0.4561810442913199
1103	30-31	0.5202757191022158
1103	32-33	0.5282976503486978
1103	34-35	0.45193772561444234
1103	36-37	0.46825489143046184
1103	38-39	0.45474147190655856
1103	40-41	0.43129472674016966
1103	42-43	0.4152599159014656
1103	44-45	0.4295010667015262
1103	46-47	0.47530061465236884
1103	48-49	0.41807411506600545
1103	50-51	0.4214293025304414
1103	52-53	0.3680288466757986
1103	54-55	0.40688953535502037
1103	56-57	0.40068095455342956
1103	58-59	0.39244911401423366
1103	60-61	0.47727981523881624
1103	62-63	0.4357428503961245
1103	64-65	0.43318040802642344
1103	66-67	0.46581717911028875
1103	68-69	0.4540120455724974
1103	70-71	0.5196936648633752
1103	72-73	0.47118344698652237
1103	74-75	0.48270781737976165
1103	76-77	0.4686253000303111
1103	78-79	0.442486134551217
1103	80-81	0.40687899700761676
1103	82-83	0.3837867698841393
1103	84-85	0.3445490526156263
1103	86-87	0.366072316698272
1103	88-89	0.32246140403470136
1103	90-91	0.25485202868627255
1103	92-93	0.21892076122215087
1103	94-95	0.3386508122795746
1103	96-97	0.4067592446012327
1103	98-99	0.3294450671808242
1103	100	0.4184774580374153
1104	1	0.05416870748461022
1104	2	0.13794964927674513
1104	3	0.2524997732114045
1104	4	0.12981546756896734
1104	5	0.1974455360458478
1104	6	0.10802826179205738
1104	7	0.10043214604818473
1104	8	0.15812008749855266
1104	9	0.18460114494036617
1104	10-11	0.21178103953985072
1104	12-13	0.17331072926052826
1104	14-15	0.15834683431253183
1104	16-17	0.13399549720591608
1104	18-19	0.22459292805169184
1104	20-21	0.2017853806041856
1104	22-23	0.08774384573484895
1104	24-25	0.12718904095491013
1104	26-27	0.18151506920990101
1104	28-29	0.08493051082336223
1104	30-31	0.1248405545035638
1104	32-33	0.1720488020189137
1104	34-35	0.1788202690969385
1104	36-37	0.16607415487163735
1104	38-39	0.10549859746319612
1104	40-41	0.1404530401180395
1104	42-43	0.1369176444934297
1104	44-45	0.07008564529355255
1104	46-47	0.010282132689759749
1104	48-49	0.09117572269190788
1104	50-51	0.10200416719767702
1104	52-53	0.07419673881276623
1104	54-55	0.14652300314023847
1104	56-57	0.16962041410622675
1104	58-59	0.09535038864975576
1104	60-61	0.21755828653820686
1104	62-63	0.1373982412083734
1104	64-65	0.023810767001094746
1104	66-67	0.07536050325572319
1104	68-69	0.05777693476733248
1104	70-71	-0.030460439010688845
1104	72-73	0.009727078425868285
1104	74-75	0.0019869301364394687
1104	76-77	0.06997739325016994
1104	78-79	0.046477641072925735
1104	80-81	-0.007925027083238945
1104	82-83	-0.002407004229333154
1104	84-85	0.07324869470620143
1104	86-87	0.11512605467027015
1104	88-89	0.045471130371264934
1104	90-91	0.03505374879566148
1104	92-93	-0.03090182530331731
1104	94-95	0.0020543167395636885
1104	96-97	0.07247734192133493
1104	98-99	0.143224068981624
1104	100	0.047523784402102365
1105	1	0.03312426111044786
1105	2	0.02899971971216786
1105	3	0.09802279842824291
1105	4	0.04580255469278427
1105	5	0.05515231948349708
1105	6	0.0668652831704506
1105	7	0.035547347343204194
1105	8	-0.004123898493446632
1105	9	0.06186760155975435
1105	10-11	0.08381545075476282
1105	12-13	0.1009723086981964
1105	14-15	0.14461622934300067
1105	16-17	0.17885365880131587
1105	18-19	0.15883693191037196
1105	20-21	0.16237281058423036
1105	22-23	0.17790197499945037
1105	24-25	0.198606342490649
1105	26-27	0.23583613098431755
1105	28-29	0.17644230341964118
1105	30-31	0.13977085411061552
1105	32-33	0.23691533993166303
1105	34-35	0.24036978008211918
1105	36-37	0.2782363417105529
1105	38-39	0.22540762981593332
1105	40-41	0.2027458573076757
1105	42-43	0.2994781727794589
1105	44-45	0.18918680180600944
1105	46-47	0.15981056415208172
1105	48-49	0.17535896015170493
1105	50-51	0.1444580787316312
1105	52-53	0.21825882182790224
1105	54-55	0.15780265508691116
1105	56-57	0.1094698900017832
1105	58-59	0.0945640579168483
1105	60-61	0.11687852526324605
1105	62-63	0.12637397760766333
1105	64-65	0.0892014816115747
1105	66-67	0.162401686071604
1105	68-69	0.11745530572599705
1105	70-71	0.16955218589674104
1105	72-73	0.10222331164482767
1105	74-75	0.06939730150446621
1105	76-77	0.024923814903416996
1105	78-79	0.1329977344473221
1105	80-81	0.1563187127165051
1105	82-83	0.07608438370517234
1105	84-85	0.08199581124171118
1105	86-87	0.05527256402787373
1105	88-89	0.03917936784091225
1105	90-91	0.05648265710113165
1105	92-93	0.058177209376033545
1105	94-95	0.06840070137201693
1105	96-97	0.09786963037564078
1105	98-99	0.11851498376454828
1105	100	0.10125885866769124
1106	1	0.003131979768227211
1106	2	0.052621222611580976
1106	3	0.07781526357219448
1106	4	0.11317254731661563
1106	5	0.19125248825381647
1106	6	0.19288274116338755
1106	7	0.15828237793559907
1106	8	0.11499395469222407
1106	9	0.18247350033941956
1106	10-11	0.23934285325834992
1106	12-13	0.21731956331052515
1106	14-15	0.2947022000958128
1106	16-17	0.20670482126162426
1106	18-19	0.21408374820187248
1106	20-21	0.2819435720295047
1106	22-23	0.2546911402110652
1106	24-25	0.3193088354839233
1106	26-27	0.3474248362476615
1106	28-29	0.293523940434703
1106	30-31	0.2842934294404671
1106	32-33	0.30460631963038765
1106	34-35	0.2717942307857797
1106	36-37	0.28126951418037294
1106	38-39	0.2581971476630329
1106	40-41	0.3116029061166117
1106	42-43	0.30038589200804466
1106	44-45	0.27160690862411485
1106	46-47	0.22820307280464647
1106	48-49	0.2731855586337275
1106	50-51	0.21503432695655533
1106	52-53	0.31661342216696653
1106	54-55	0.2692394607169035
1106	56-57	0.2623217025544591
1106	58-59	0.27313986725014416
1106	60-61	0.34174700047633166
1106	62-63	0.23098747707712164
1106	64-65	0.27374298255754326
1106	66-67	0.3061268993177606
1106	68-69	0.31258272066061465
1106	70-71	0.28298461656359564
1106	72-73	0.2624671501961231
1106	74-75	0.22800956674976902
1106	76-77	0.20353539517333274
1106	78-79	0.19694957306712269
1106	80-81	0.18375191334641983
1106	82-83	0.19967214522465326
1106	84-85	0.148649243507478
1106	86-87	0.1808598828502994
1106	88-89	0.14784988770551521
1106	90-91	0.1670132022464017
1106	92-93	0.07832671159554394
1106	94-95	0.03740715092262903
1106	96-97	0.12286514315265151
1106	98-99	0.09076434778393505
1106	100	0.014720464211933404
1107	1	-0.010087427970635332
1107	2	0.04443789397682707
1107	3	0.04070806994467091
1107	4	-0.024697615894098135
1107	5	0.031448748983073926
1107	6	-0.13189577572451583
1107	7	0.038548937434626396
1107	8	0.013100326567922593
1107	9	0.034013535248874405
1107	10-11	-0.05580221293166687
1107	12-13	-0.04490324540124391
1107	14-15	0.028594364662119176
1107	16-17	-0.006622253961666047
1107	18-19	-0.03262984429319715
1107	20-21	-0.007939283348171955
1107	22-23	0.012211369101947867
1107	24-25	0.011819497264063727
1107	26-27	0.08639746651331848
1107	28-29	0.04083195341478074
1107	30-31	0.03491664123050242
1107	32-33	0.08589077172585746
1107	34-35	0.07842672777168502
1107	36-37	0.1081554946142802
1107	38-39	0.014728275262498869
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2216	26-27	-0.16250466606132363
2216	28-29	-0.1693043915211021
2216	30-31	-0.11465253658577268
2216	32-33	-0.2034528256248862
2216	34-35	-0.1019432906355604
2216	36-37	-0.12399826863342867
2216	38-39	-0.08891212873108145
2216	40-41	-0.13937926755556163
2216	42-43	-0.09139115108724383
2216	44-45	-6.726692252030375E-4
2216	46-47	0.01744073695198267
2216	48-49	0.005335409989818629
2216	50-51	-0.006206384664324105
2216	52-53	0.021704015877276106
2216	54-55	0.01577415308021557
2216	56-57	-0.019033607598572644
2216	58-59	0.0030410133349292323
2216	60-61	0.008634171348688824
2216	62-63	0.028816599744899918
2216	64-65	0.02193100889073918
2216	66-67	-0.003617407307409337
2216	68-69	0.07519368142041571
2216	70-71	0.09856512347462143
2216	72-73	0.04568913735973723
2216	74-75	0.02315613689984275
2216	76-77	-0.013200396627723876
2216	78-79	0.08797369923092901
2216	80-81	0.039494919026623165
2216	82-83	0.07829373036842213
2216	84-85	0.04387936058257935
2216	86-87	0.09227522743660899
2216	88-89	0.12163092315030255
2216	90-91	0.0964592662413537
2216	92-93	0.03344346345023297
2216	94-95	0.07560376070776442
2216	96-97	0.10839531304134553
2216	98-99	0.12411205317758345
2216	100	0.17387039414192174
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33377.0
3	4618.0
4	3166.0
5	2616.0
6	2116.0
7	2339.0
8	2625.0
9	2857.0
10	3070.0
11	3286.0
12	3539.0
13	3801.0
14	4175.0
15	4209.0
16	4183.0
17	4347.0
18	4061.0
19	4107.0
20	3988.0
21	4230.0
22	4967.0
23	7021.0
24	10646.0
25	14500.0
26	15797.0
27	13672.0
28	13979.0
29	17527.0
30	21080.0
31	25285.0
32	32841.0
33	44477.0
34	65280.0
35	104381.0
36	194138.0
37	393007.0
38	588344.0
39	237696.0
40	371.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.19971232904768	19.114364169999984	12.963677735013986	23.72224576593835
2	15.822281926282175	19.632056808949866	39.91477817275118	24.63088309201678
3	18.823220493860873	25.073312396937265	29.186263903622557	26.917203205579305
4	12.436250580972462	16.267123917159235	37.96075383310532	33.335871668762984
5	13.402455458190094	37.77275720709224	34.795548287281676	14.02923904743599
6	31.619006306166263	37.08496309149193	17.63084979896732	13.665180803374492
7	28.30809853884842	32.04959817412507	21.86717348070012	17.775129806326387
8	26.022654287630363	35.98963757236028	19.74485699146145	18.242851148547913
9	25.360651278178587	17.240050120943557	20.45353572167542	36.945762879202434
10-11	24.544848230227917	26.84421279277387	27.92240836046634	20.688530616531878
12-13	25.01497991037786	24.118548463751768	28.126127431905086	22.74034419396529
14-15	22.274237779101142	25.965215482518467	25.242826789508456	26.51771994887194
16-17	22.28903736308979	29.369560548223745	25.785804346992713	22.55559774169375
18-19	22.312559898123958	28.43173911331084	27.42810255871657	21.82759842984863
20-21	23.371022884961317	27.56742620788742	27.124451664416917	21.937099242734348
22-23	22.895621569504748	27.37897559346386	27.066253064208155	22.65914977282323
24-25	22.766900749248062	27.48266042796545	27.140162032934356	22.610276789852133
26-27	22.55008713374485	27.56829240178516	27.159471426249564	22.72214903822043
28-29	22.535424644125683	27.633795509587657	27.21065322917739	22.620126617109275
30-31	22.736555873152078	27.949652169666212	26.863545585020322	22.450246372161388
32-33	22.442117965972102	27.65582028934059	27.312458743838157	22.58960300084915
34-35	22.544087317372234	27.50161146972603	27.372851428398885	22.581449784502848
36-37	22.8425224449395	27.63294663755837	26.89801024741616	22.626520670085974
38-39	22.73289740213537	27.829721650797318	27.009967954987324	22.427412992079983
40-41	22.506989430259033	27.695367966459138	27.20705307555936	22.590589527722464
42-43	22.478201607331957	27.59711828229165	27.167334090777207	22.75734601959919
44-45	22.544090095696482	27.6483400487607	27.159551464593353	22.648018390949467
46-47	22.61013096712325	27.731177611753743	27.131222356315867	22.52746906480714
48-49	22.551789460471582	27.61279137767094	27.18253098150576	22.652888180351713
50-51	22.47306288023932	27.666743344761517	27.175110710564347	22.685083064434806
52-53	22.636800047057203	27.48490007302795	27.177216330005454	22.70108354990939
54-55	22.58358455090701	27.8442118420638	27.112590989779733	22.459612617249455
56-57	22.369512185514978	27.622084916535588	27.15892768518543	22.849475212764002
58-59	22.440266495249258	27.271385687066598	27.36575979295466	22.92258802472948
60-61	22.587767898874574	27.69445480182967	27.1357074210811	22.582069878214657
62-63	22.381622470691013	27.743013095334064	27.125336403117085	22.750028030857838
64-65	22.36931763129156	27.54202162256124	27.147282396489597	22.941378349657608
66-67	22.29443765528346	27.530578795375853	27.145767283432914	23.029216265907774
68-69	22.6121064487745	27.570242048626092	27.204809830565175	22.612841672034236
70-71	22.519819180177038	27.399018808790597	27.23876913123301	22.842392879799352
72-73	22.44309200541536	27.54115346279357	27.26379493061179	22.751959601179276
74-75	22.40062366546828	27.62077610535724	27.161849486352985	22.816750742821494
76-77	22.46535136237947	27.58670397671282	27.048321796259735	22.899622864647977
78-79	22.54154007253906	27.604780026210268	27.1940071908878	22.659672710362877
80-81	22.534328118361703	27.377025591364756	27.322146815691863	22.766499474581675
82-83	22.383513998853594	27.43001754796413	27.434822691515826	22.751645761666456
84-85	22.382654986321203	27.43549933319312	27.175813566100004	23.006032114385672
86-87	22.572519689298485	27.487032659789612	27.162566884797712	22.77788076611419
88-89	22.316172715449913	27.37805129411604	27.252227710228706	23.05354828020534
90-91	22.2873667760325	27.34943118564605	27.2968137574095	23.06638828091195
92-93	22.56820310957448	27.426151807056826	27.24227962644777	22.763365456920926
94-95	22.359469727800956	27.252488440664706	27.2868841913454	23.10115764018894
96-97	22.431273825830765	27.20496010506668	27.37515258115191	22.98861348795065
98-99	22.25207829982445	27.38792461985138	27.26207923979203	23.097917840532137
100	22.484325278049088	27.120472184588884	27.09573920623418	23.299463331127846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1869.0
1	1194.0
2	463.5
3	346.5
4	277.5
5	236.0
6	199.0
7	197.0
8	192.5
9	191.0
10	191.5
11	189.0
12	201.0
13	220.5
14	256.0
15	290.0
16	340.5
17	441.0
18	571.0
19	776.5
20	1122.0
21	1641.0
22	2428.0
23	3763.0
24	5607.5
25	7760.5
26	10559.0
27	14360.5
28	19140.0
29	23711.5
30	27479.5
31	31585.0
32	36037.5
33	40958.0
34	45485.0
35	48706.5
36	51865.0
37	55044.0
38	57627.0
39	59198.0
40	60607.0
41	62583.0
42	64420.5
43	65343.5
44	65073.0
45	65232.5
46	64640.0
47	62910.0
48	63289.5
49	63267.5
50	61111.5
51	59373.0
52	56915.5
53	55584.5
54	54930.5
55	52623.5
56	48841.5
57	45268.5
58	41244.0
59	35903.0
60	31998.5
61	28518.0
62	25376.5
63	21856.5
64	18083.0
65	15599.0
66	13053.5
67	10483.0
68	8732.0
69	7114.0
70	5652.5
71	4342.0
72	3341.0
73	2699.5
74	2182.5
75	1611.5
76	1074.0
77	788.0
78	648.0
79	472.0
80	310.5
81	213.5
82	152.5
83	106.0
84	81.0
85	63.0
86	48.5
87	37.5
88	30.0
89	23.5
90	17.5
91	13.5
92	12.0
93	8.0
94	6.0
95	5.0
96	3.5
97	2.5
98	1.5
99	1.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07661150463420893
2	0.07934013356638622
3	0.08075692166578598
4	0.08311823516478557
5	0.08149155253214141
6	0.0813866052655192
7	0.08059950076585268
8	0.08038960623260827
9	0.08002229079943056
10-11	0.07970744899956395
12-13	0.0788153972332752
14-15	0.07771345093374207
16-17	0.07661150463420893
18-19	0.0757719265012313
20-21	0.07522095335146473
22-23	0.07739860913387546
24-25	0.0774773195838421
26-27	0.07674268871748668
28-29	0.0732007184689873
30-31	0.07797581910029758
32-33	0.07650655736758673
34-35	0.07354179708550945
36-37	0.07013101092028783
38-39	0.0717052199196209
40-41	0.07425019113520934
42-43	0.07910400221648628
44-45	0.0798386330828417
46-47	0.08275091973160785
48-49	0.08603052181355174
50-51	0.08684386312987381
52-53	0.08676515267990716
54-55	0.08705375766311822
56-57	0.08469244416411864
58-59	0.0836954451312077
60-61	0.081281657998897
62-63	0.08225242021515239
64-65	0.08136036844886366
66-67	0.08080939529909709
68-69	0.08057326394919713
70-71	0.07886787086658631
72-73	0.07897281813320851
74-75	0.07834313453347529
76-77	0.07847431861675305
78-79	0.07889410768324186
80-81	0.07986486989949725
82-83	0.07920894948310848
84-85	0.07842184498344194
86-87	0.07934013356638622
88-89	0.07690010961741998
90-91	0.07383040206872052
92-93	0.07383040206872052
94-95	0.07419771750189824
96-97	0.07369921798544277
98-99	0.0733581393689206
100	0.07236114033600966
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1905719.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.84865704994559
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.23940818414204	54.30745336642254
2	11.809027985223054	15.788353237547717
3	3.194640029535006	6.406721871972411
4	1.2786888732819655	3.4191453585442977
5	0.6699646540121413	2.2393118695821546
6	0.4052216536497722	1.6253114012427274
7	0.2629724678495936	1.230554942179873
8	0.1982196998321828	1.0600576587699795
9	0.13906735656679803	0.8366819423378741
>10	0.7389577402022605	8.76142425713852
>50	0.0469328817649539	2.137449230707691
>100	0.016191445121620514	1.8502279710992795
>500	6.284700609079804E-4	0.2703496625187662
>1k	7.85587576134961E-5	0.06695722993617365
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.2473633311102004E-5	0.0
10-11	0.0	0.0	0.0	5.2473633311102004E-5	0.0
12-13	0.0	0.0	0.0	5.2473633311102004E-5	0.0
14-15	0.0	0.0	0.0	5.2473633311102004E-5	0.0
16-17	0.0	0.0	0.0	5.2473633311102004E-5	0.0
18-19	0.0	2.6236816655551002E-5	0.0	5.2473633311102004E-5	0.0
20-21	0.0	5.2473633311102004E-5	0.0	5.2473633311102004E-5	0.0
22-23	0.0	5.2473633311102004E-5	0.0	5.2473633311102004E-5	0.0
24-25	0.0	5.2473633311102004E-5	0.0	1.83657716588857E-4	0.0
26-27	0.0	5.2473633311102004E-5	0.0	3.93552249833265E-4	0.0
28-29	0.0	1.31184083277755E-4	0.0	0.0012856040161219992	0.0
30-31	0.0	1.57420899933306E-4	0.0	0.0057196260309101184	0.0
32-33	0.0	1.57420899933306E-4	0.0	0.014876275043697419	0.0
34-35	0.0	1.57420899933306E-4	0.0	0.025134870356017858	0.0
36-37	0.0	1.57420899933306E-4	0.0	0.04294966886513699	0.0
38-39	0.0	1.57420899933306E-4	0.0	0.07792334546698648	0.0
40-41	0.0	1.57420899933306E-4	0.0	0.12368035371426742	0.0
42-43	2.6236816655551002E-5	1.57420899933306E-4	0.0	0.16993586147800382	0.0
44-45	5.2473633311102004E-5	1.57420899933306E-4	0.0	0.2217273375560615	0.0
46-47	5.2473633311102004E-5	2.0989453324440801E-4	0.0	0.28519419704583937	0.0
48-49	5.2473633311102004E-5	2.0989453324440801E-4	0.0	0.35508907661622724	0.0
50-51	1.0494726662220401E-4	2.0989453324440801E-4	0.0	0.4375251545479685	0.0
52-53	1.0494726662220401E-4	2.0989453324440801E-4	0.0	0.5241853599612535	0.0
54-55	1.0494726662220401E-4	2.0989453324440801E-4	0.0	0.6148597983228378	0.0
56-57	1.0494726662220401E-4	2.6236816655551E-4	0.0	0.7191773813453085	0.0
58-59	1.31184083277755E-4	2.6236816655551E-4	0.0	0.843959681359109	0.0
60-61	1.57420899933306E-4	2.6236816655551E-4	0.0	0.9791317607685079	0.0
62-63	1.57420899933306E-4	2.6236816655551E-4	0.0	1.1144874978944954	0.0
64-65	1.57420899933306E-4	2.6236816655551E-4	0.0	1.256087597384504	0.0
66-67	1.57420899933306E-4	2.6236816655551E-4	0.0	1.4140332336509212	0.0
68-69	1.57420899933306E-4	3.14841799866612E-4	0.0	1.595067268574223	0.0
70-71	1.57420899933306E-4	3.14841799866612E-4	0.0	1.7958051528058439	0.0
72-73	1.57420899933306E-4	3.14841799866612E-4	0.0	2.001318137668775	0.0
74-75	1.57420899933306E-4	3.4107861652216305E-4	0.0	2.2114488022630825	0.0
76-77	1.57420899933306E-4	3.67315433177714E-4	0.0	2.431733114903089	0.0
78-79	2.0989453324440801E-4	3.67315433177714E-4	0.0	2.6929206247090995	0.0
80-81	2.0989453324440801E-4	3.67315433177714E-4	0.0	2.978560847638083	0.0
82-83	2.0989453324440801E-4	3.67315433177714E-4	0.0	3.273934929546276	0.0
84-85	2.3613134989995902E-4	3.67315433177714E-4	0.0	3.559286547492049	0.0
86-87	2.6236816655551E-4	3.67315433177714E-4	0.0	3.854503208500309	0.0
88	2.6236816655551E-4	3.67315433177714E-4	0.0	4.095147290865023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	6735	0.0	28.33606	1
GTATCAA	3675	0.0	28.139498	1
TCAACGC	3800	0.0	26.224262	4
TACATGG	6930	0.0	26.182135	2
ATCAACG	3865	0.0	25.783234	3
CAACGCA	3880	0.0	25.682205	5
GGTATCA	2850	0.0	24.9048	1
AACGCAG	4015	0.0	24.700953	6
ACATGGG	6955	0.0	24.668726	3
TATCAAC	4260	0.0	23.944242	2
ACGCAGA	4690	0.0	21.04514	7
CTTACGC	115	0.003956773	20.437283	3
CGCAGAG	4920	0.0	19.774733	8
CATGGGG	4435	0.0	19.713799	4
GAGTACA	4730	0.0	19.676826	1
ACACCGT	320	4.5656634E-10	19.094584	6
TATACCG	125	0.006410097	18.801313	5
GACTCGT	200	1.4080282E-5	18.800325	7
AGTACAT	4605	0.0	18.169434	2
GTATAGA	820	0.0	17.770468	1
>>END_MODULE