FastQCFastQC Report
Wed 25 May 2016
SRR1032917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905719
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47330.24835770646144578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37800.19835033391596557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19720.10347800488949316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41500.037.5861051
GTATCAA63850.036.570661
ATCAACG75850.030.3275953
TCAACGC76550.030.111724
CAACGCA77700.029.7840085
TATCAAC78750.029.449712
AACGCAG79900.028.8461826
GTACATG68900.028.16231
TACATGG67800.027.3370232
ACATGGG66450.026.7597143
ACACCGT3500.025.5343366
ACGCAGA91150.025.2845737
CGCAGAG94350.024.327318
TACGTCG1000.001751078823.518475
GCAGAGT100450.022.44719
ACGTCGC1100.003046359421.3804266
GAGTACT59800.021.01316812-13
AGAGTAC85800.020.53113410-11
CATGGGG44500.019.6624494
AAACGCG600.00504864719.5951242-43
Produced by FastQC (version 0.11.5)