FastQCFastQC Report
Wed 25 May 2016
SRR1032916_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032916_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3006134
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39650.035.5631
TCAACGC39200.034.1726844
GGTATCA23800.034.1656341
ATCAACG39750.033.6998563
CAACGCA40950.032.7101365
AACGCAG43050.031.1146
TATCAAC49300.027.7438282
GTACATG88100.027.7426381
TACATGG86250.026.8118212
ACGCAGA51750.025.3379087
ACATGGG89700.024.6278063
CGCAGAG53550.024.4862178
CATGGGG54900.020.547514
TAGACCG1200.005066433519.5830425
GCAGAGT66200.019.0972679
GAGTACA64400.017.735391
CCGATCG1952.469349E-416.8709839
GACGCGT850.001920527316.58743710-11
AGAGTAC56200.016.18160410-11
GTCTACG3201.8097126E-716.1570851
Produced by FastQC (version 0.11.5)