##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032916_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3006134
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.649513960455522	33.0	31.0	34.0	30.0	34.0
2	31.69310716022639	34.0	31.0	34.0	30.0	34.0
3	31.75128221163794	34.0	31.0	34.0	30.0	34.0
4	35.090742461912875	37.0	35.0	37.0	33.0	37.0
5	35.13420925347972	37.0	35.0	37.0	33.0	37.0
6	35.20927543482759	37.0	36.0	37.0	33.0	37.0
7	35.107889069482596	37.0	36.0	37.0	33.0	37.0
8	35.115784259783496	37.0	36.0	37.0	33.0	37.0
9	36.73807554819579	39.0	38.0	39.0	34.0	39.0
10-11	36.74259946496065	39.0	38.0	39.0	33.5	39.0
12-13	36.72163865616103	39.0	38.0	39.0	33.0	39.0
14-15	38.21147227635228	41.0	39.0	41.0	34.0	41.0
16-17	38.253877571658485	41.0	39.0	41.0	34.0	41.0
18-19	38.27313353296959	41.0	39.0	41.0	34.0	41.0
20-21	38.30750542058338	41.0	39.0	41.0	34.0	41.0
22-23	38.31144503205779	41.0	39.0	41.0	34.0	41.0
24-25	38.35531865844969	41.0	39.0	41.0	34.0	41.0
26-27	38.34188362860738	41.0	39.0	41.0	34.0	41.0
28-29	38.329479989913956	41.0	39.0	41.0	34.0	41.0
30-31	38.29581665354904	41.0	39.0	41.0	34.0	41.0
32-33	38.24417524301977	41.0	39.0	41.0	33.5	41.0
34-35	38.17675808862812	41.0	39.0	41.0	33.0	41.0
36-37	38.0946280172474	41.0	38.5	41.0	33.0	41.0
38-39	38.00246994977603	41.0	38.0	41.0	33.0	41.0
40-41	37.91826861344172	40.5	38.0	41.0	33.0	41.0
42-43	37.817455076852866	40.0	38.0	41.0	33.0	41.0
44-45	37.70212688456336	40.0	38.0	41.0	32.5	41.0
46-47	37.58598053180597	40.0	38.0	41.0	32.0	41.0
48-49	37.46274301145591	40.0	38.0	41.0	32.0	41.0
50-51	37.341577421365784	40.0	37.5	41.0	31.5	41.0
52-53	37.1959204080723	40.0	37.0	41.0	31.0	41.0
54-55	37.04494160938934	40.0	37.0	41.0	31.0	41.0
56-57	36.878497265923606	40.0	36.5	41.0	31.0	41.0
58-59	36.68460554319934	40.0	36.0	41.0	30.5	41.0
60-61	36.46262741447986	39.5	35.5	41.0	30.0	41.0
62-63	36.22572979115369	39.0	35.0	41.0	30.0	41.0
64-65	35.96158105393838	39.0	35.0	41.0	30.0	41.0
66-67	35.686171175336824	38.0	35.0	40.0	30.0	41.0
68-69	35.38504404660604	37.5	35.0	40.0	29.0	41.0
70-71	35.053705856092904	37.0	35.0	40.0	29.0	41.0
72-73	34.76962204612303	37.0	34.0	39.0	29.0	41.0
74-75	34.49642231517291	36.0	34.0	39.0	29.0	41.0
76-77	34.164286089708575	36.0	34.0	39.0	29.0	40.5
78-79	33.791691088953456	35.0	34.0	37.5	28.5	39.5
80-81	33.4539285341239	35.0	34.0	37.0	28.0	39.0
82-83	33.122056601601926	35.0	34.0	37.0	28.0	39.0
84-85	32.93563926292042	35.0	34.0	36.0	28.0	38.0
86-87	32.68617683044069	35.0	34.0	36.0	27.5	37.0
88-89	32.43444038090119	35.0	34.0	36.0	27.0	37.0
90-91	32.22443161216366	35.0	34.0	35.0	27.0	36.5
92-93	32.026590631023105	35.0	34.0	35.0	27.0	36.0
94-95	31.840905960945186	35.0	33.0	35.0	26.0	36.0
96-97	31.666587384328178	35.0	33.0	35.0	25.5	36.0
98-99	31.487875789968115	35.0	33.0	35.0	25.0	36.0
100	31.35865733197522	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3210473569016905
1101	2	0.3842841121104037
1101	3	0.3473913931395991
1101	4	0.359293696958062
1101	5	0.2801074081728743
1101	6	0.3260915715622872
1101	7	0.29574082954704295
1101	8	0.3272303972240209
1101	9	0.35491408805987845
1101	10-11	0.35403711444286756
1101	12-13	0.17915299879305024
1101	14-15	0.23113385421341803
1101	16-17	0.22479814759070393
1101	18-19	0.2998357245977914
1101	20-21	0.2998470795539063
1101	22-23	0.2811161412083152
1101	24-25	0.24405351475536463
1101	26-27	0.2619367576246745
1101	28-29	0.263378794478605
1101	30-31	0.24479785474667892
1101	32-33	0.27534354161964103
1101	34-35	0.2907471955669365
1101	36-37	0.3385978822486493
1101	38-39	0.3138592862969034
1101	40-41	0.3524104435742146
1101	42-43	0.32234261887276716
1101	44-45	0.31163646911767273
1101	46-47	0.31968797100874724
1101	48-49	0.33622582584022354
1101	50-51	0.31599733372435423
1101	52-53	0.3116535374159781
1101	54-55	0.3035979425431492
1101	56-57	0.37187930816254067
1101	58-59	0.3344049619837506
1101	60-61	0.36288205188169087
1101	62-63	0.4059875589572215
1101	64-65	0.3484229688083573
1101	66-67	0.3589237734171675
1101	68-69	0.3958624231765455
1101	70-71	0.37519395304884995
1101	72-73	0.2983019773721125
1101	74-75	0.2821522748142442
1101	76-77	0.3196006548590944
1101	78-79	0.3763011616504315
1101	80-81	0.31253866704886946
1101	82-83	0.40280176587953065
1101	84-85	0.34678403670423563
1101	86-87	0.33289482423253247
1101	88-89	0.3530530234244367
1101	90-91	0.35982036047214194
1101	92-93	0.3244408169708777
1101	94-95	0.35787361315728106
1101	96-97	0.37523367333607993
1101	98-99	0.3880622849876225
1101	100	0.3984649609831443
1102	1	0.32910992241494696
1102	2	0.26417827856555576
1102	3	0.3309668251532507
1102	4	0.29504284577683393
1102	5	0.23192790387748374
1102	6	0.2901420813878204
1102	7	0.3418162349122582
1102	8	0.3244305154638596
1102	9	0.4197935375782791
1102	10-11	0.3448617105943228
1102	12-13	0.3821353811673518
1102	14-15	0.42498919582829586
1102	16-17	0.3921846263551956
1102	18-19	0.3739273375671104
1102	20-21	0.35983116724483466
1102	22-23	0.38322588046852957
1102	24-25	0.3660968260335409
1102	26-27	0.35655478637318083
1102	28-29	0.3871297674507659
1102	30-31	0.38183244452748255
1102	32-33	0.44937193538216746
1102	34-35	0.451505220596232
1102	36-37	0.4566000127828218
1102	38-39	0.47672457761233034
1102	40-41	0.4460993406332605
1102	42-43	0.40780211603429706
1102	44-45	0.4629430734995026
1102	46-47	0.3887767452352904
1102	48-49	0.40184479206518375
1102	50-51	0.41483772206899516
1102	52-53	0.36285393791595766
1102	54-55	0.38934597365073387
1102	56-57	0.3482531777438638
1102	58-59	0.2536683580264736
1102	60-61	0.34488534849014485
1102	62-63	0.3264683160819928
1102	64-65	0.31721839105315297
1102	66-67	0.35741717791611194
1102	68-69	0.28195461796480004
1102	70-71	0.3490640277418038
1102	72-73	0.32606461376713725
1102	74-75	0.3881078846858941
1102	76-77	0.3189515600742041
1102	78-79	0.34425361037900615
1102	80-81	0.340775310445828
1102	82-83	0.3905447378668896
1102	84-85	0.3507365314812887
1102	86-87	0.38965755863286233
1102	88-89	0.34394032713287714
1102	90-91	0.35489959888627
1102	92-93	0.3525159556690909
1102	94-95	0.40331347636647763
1102	96-97	0.40185228512173055
1102	98-99	0.36933894750078977
1102	100	0.3274376735415103
1103	1	0.1287671867120146
1103	2	0.15131790607521012
1103	3	0.170443350992052
1103	4	0.07129994572179044
1103	5	0.0910783991502413
1103	6	0.0945891870814961
1103	7	0.07991522105076854
1103	8	0.07754033178530051
1103	9	0.18402446311709753
1103	10-11	0.1666739038084657
1103	12-13	0.137424584922897
1103	14-15	0.171474869236242
1103	16-17	0.17384547391459648
1103	18-19	0.14287983775879098
1103	20-21	0.16971593577374477
1103	22-23	0.1119104259830408
1103	24-25	0.18672725123268208
1103	26-27	0.24354647836573662
1103	28-29	0.2537923968681639
1103	30-31	0.28079714023698443
1103	32-33	0.2871447594822385
1103	34-35	0.22757523417745062
1103	36-37	0.23978468924615726
1103	38-39	0.2617794487164602
1103	40-41	0.2825810593984812
1103	42-43	0.27600096533681295
1103	44-45	0.1740599709467432
1103	46-47	0.23053859267124466
1103	48-49	0.25890740700899073
1103	50-51	0.21000441916046952
1103	52-53	0.249086086031582
1103	54-55	0.22456107817534843
1103	56-57	0.3172996632369305
1103	58-59	0.3237605608881253
1103	60-61	0.3577603632807822
1103	62-63	0.29473294811464257
1103	64-65	0.28142596570067013
1103	66-67	0.2554584546595464
1103	68-69	0.3326048768781362
1103	70-71	0.3466416200077802
1103	72-73	0.34623538592072833
1103	74-75	0.3472520273992785
1103	76-77	0.3396879834273747
1103	78-79	0.343219487013009
1103	80-81	0.2766705111337586
1103	82-83	0.28397031594688116
1103	84-85	0.3230359113155927
1103	86-87	0.3015755417394743
1103	88-89	0.31137264008927446
1103	90-91	0.2825141046414501
1103	92-93	0.3012611564908312
1103	94-95	0.26910146539866275
1103	96-97	0.27732205165121115
1103	98-99	0.28485016971576016
1103	100	0.255127106919975
1104	1	-0.03918627412188869
1104	2	-0.05963719586829086
1104	3	-0.0868307116615128
1104	4	-0.09830555219404147
1104	5	-0.11733490350666642
1104	6	-0.04449915600710597
1104	7	-0.14045010133654046
1104	8	-0.11718844803183259
1104	9	0.009680079115995
1104	10-11	-0.010057804574707063
1104	12-13	-0.03474009691509394
1104	14-15	-0.02923737100419288
1104	16-17	-0.10394709460074125
1104	18-19	-0.09686504850926525
1104	20-21	-0.07933916789166062
1104	22-23	0.003102972246040281
1104	24-25	0.00452468082325197
1104	26-27	0.025225423552136306
1104	28-29	0.01328639469855375
1104	30-31	-0.03645606747663521
1104	32-33	-0.007892208531359302
1104	34-35	-0.009399534590343706
1104	36-37	4.7094193995178557E-4
1104	38-39	-0.008428286088701498
1104	40-41	0.0309629637478821
1104	42-43	-0.0021369779382780507
1104	44-45	-0.055943724530074235
1104	46-47	-0.055650423682891415
1104	48-49	-0.05851236207006849
1104	50-51	-0.021605285506041128
1104	52-53	-0.0827323004888072
1104	54-55	-0.08787608878471076
1104	56-57	-0.06867798906241518
1104	58-59	-0.10871387210784178
1104	60-61	-0.02364893792971401
1104	62-63	-0.05775792246816991
1104	64-65	-0.026330429527270383
1104	66-67	-0.08700181234196691
1104	68-69	-0.031268128524686745
1104	70-71	-0.12150409833860465
1104	72-73	-0.17007554143201986
1104	74-75	-0.15780060996583245
1104	76-77	-0.11443396333391576
1104	78-79	-0.10133081054572557
1104	80-81	-0.1280906603986125
1104	82-83	-0.0956031677486493
1104	84-85	-0.06717746604557817
1104	86-87	-0.10180843552075913
1104	88-89	-0.15091097914680773
1104	90-91	-0.1568524257296744
1104	92-93	-0.1872442880458891
1104	94-95	-0.14937734256502466
1104	96-97	-0.12494384519068191
1104	98-99	-0.1294068208945518
1104	100	-0.11929920010659956
1105	1	0.03502548106022729
1105	2	0.004631983848305765
1105	3	6.206123690795096E-4
1105	4	0.00594325895509229
1105	5	0.02088900247782277
1105	6	0.03582548593719537
1105	7	0.00450404516846703
1105	8	-0.015133280213326827
1105	9	0.06943187826574615
1105	10-11	0.07508512919395827
1105	12-13	0.07255249570324196
1105	14-15	0.08314246718868645
1105	16-17	0.05362731900795836
1105	18-19	0.045753067385611246
1105	20-21	0.08264767193309552
1105	22-23	0.10316332297205122
1105	24-25	0.14458190529425963
1105	26-27	0.08626012972101904
1105	28-29	0.10656619947030777
1105	30-31	0.08236602513254354
1105	32-33	0.10955144675043726
1105	34-35	0.10025072708215532
1105	36-37	0.15187489788976194
1105	38-39	0.07084057352633977
1105	40-41	0.07896442968664985
1105	42-43	0.1498156520856
1105	44-45	0.1274868922993022
1105	46-47	0.07812493936386744
1105	48-49	0.08524701064038709
1105	50-51	0.15965343173330382
1105	52-53	0.16221688654801625
1105	54-55	0.07935941624815257
1105	56-57	0.08495801713997508
1105	58-59	0.09917366189505117
1105	60-61	0.11021837534209311
1105	62-63	0.1450275191445698
1105	64-65	0.1258588018773068
1105	66-67	0.13554392480032362
1105	68-69	0.08834808445067921
1105	70-71	0.08328176740869964
1105	72-73	0.023502479005202304
1105	74-75	0.084902838265009
1105	76-77	0.08459136428054848
1105	78-79	0.12704311777216049
1105	80-81	0.15857126055188786
1105	82-83	0.11012110098251071
1105	84-85	0.10907969069867818
1105	86-87	0.06647161143308722
1105	88-89	0.11534286180862807
1105	90-91	0.0766671614319776
1105	92-93	0.09007050951198892
1105	94-95	0.09944567875984234
1105	96-97	0.08862117427970873
1105	98-99	0.1267729291861599
1105	100	0.11984946242696637
1106	1	-0.03573736552962714
1106	2	-0.06026146673403687
1106	3	-0.09458891493969546
1106	4	-0.07132873536484396
1106	5	-0.054896617184652996
1106	6	-0.05931473451855851
1106	7	-0.02888726707009681
1106	8	-0.07242121560914683
1106	9	-0.10852113708455136
1106	10-11	-0.08140472304852864
1106	12-13	-0.07231379278885441
1106	14-15	-0.008573759874160203
1106	16-17	-0.054839729830789
1106	18-19	-0.048579330979784174
1106	20-21	-0.06769488230976606
1106	22-23	-0.07040764542157518
1106	24-25	-0.07276293667437983
1106	26-27	-0.06317441480327801
1106	28-29	-0.06575896949575366
1106	30-31	-0.04440520343911203
1106	32-33	-0.05827427334751434
1106	34-35	-0.020163259190695726
1106	36-37	0.013659718749536864
1106	38-39	-0.021255142285781403
1106	40-41	-0.006402999820110722
1106	42-43	-0.016786554710556345
1106	44-45	-0.051339554296362166
1106	46-47	-0.037064749295467436
1106	48-49	0.03611070045030118
1106	50-51	-0.008924869845046146
1106	52-53	0.04555543913209448
1106	54-55	5.955506926582643E-4
1106	56-57	-0.016690601512479475
1106	58-59	-0.051034251168672995
1106	60-61	0.01755293920935941
1106	62-63	-0.0012275797436132052
1106	64-65	0.08099690565079953
1106	66-67	0.004701031043829573
1106	68-69	-0.014198025468594722
1106	70-71	0.02226552806849469
1106	72-73	0.008792636617577898
1106	74-75	-0.05115530873557361
1106	76-77	-0.03283942917898486
1106	78-79	-0.04259442437597727
1106	80-81	-0.03973071155537866
1106	82-83	-0.052120070776012994
1106	84-85	-0.021807825278244763
1106	86-87	-0.029748261884783744
1106	88-89	-0.02946091559974917
1106	90-91	-0.05047775949750388
1106	92-93	-0.016578621071765554
1106	94-95	-0.035183257995576867
1106	96-97	0.018391445797174555
1106	98-99	0.04695621907903558
1106	100	0.07892758833968117
1107	1	0.017567913765788035
1107	2	0.059102033380899144
1107	3	0.13270977293146657
1107	4	0.04559061029007694
1107	5	0.04922966422567754
1107	6	-0.005492784667325168
1107	7	0.043498755796377964
1107	8	0.07576068197536756
1107	9	0.02891496893155221
1107	10-11	-0.007391557292002915
1107	12-13	-0.03042531048652819
1107	14-15	0.041551757611166806
1107	16-17	0.04836742261628757
1107	18-19	0.008346987627341207
1107	20-21	0.0031582208784897148
1107	22-23	-0.028479402517177732
1107	24-25	-0.03357745107575738
1107	26-27	-0.09462903434067016
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2216	9	0.12962662733551866
2216	10-11	0.06213895695004368
2216	12-13	0.06440657761459079
2216	14-15	-0.026695483713005785
2216	16-17	0.028850309349174097
2216	18-19	0.05475471191968495
2216	20-21	0.050116976971864347
2216	22-23	0.08325659383420714
2216	24-25	0.16209973498604313
2216	26-27	0.083674454058972
2216	28-29	0.05717106976359787
2216	30-31	0.0965826118712414
2216	32-33	0.012799444124084403
2216	34-35	0.05973357832759518
2216	36-37	0.046447628933350416
2216	38-39	0.12934623982997095
2216	40-41	0.13784038434395995
2216	42-43	0.1471719758623209
2216	44-45	0.044477553293702954
2216	46-47	0.10968177829624182
2216	48-49	0.1953022986909687
2216	50-51	0.2163819182715585
2216	52-53	0.22539807919449828
2216	54-55	0.17101894831367304
2216	56-57	0.16607800648746007
2216	58-59	0.25908367822800926
2216	60-61	0.24266577511201604
2216	62-63	0.23470341073132772
2216	64-65	0.26472925829425975
2216	66-67	0.28107648531501894
2216	68-69	0.27269234308899115
2216	70-71	0.224083568018564
2216	72-73	0.21430178505881514
2216	74-75	0.2583003796031278
2216	76-77	0.25721223021339057
2216	78-79	0.3139684545968535
2216	80-81	0.3220304216862573
2216	82-83	0.26572177139662756
2216	84-85	0.23558630050649754
2216	86-87	0.3528968791253604
2216	88-89	0.32250324287465304
2216	90-91	0.28970232888566017
2216	92-93	0.29754568995439
2216	94-95	0.31510864411803396
2216	96-97	0.3700254451302776
2216	98-99	0.4245254191449348
2216	100	0.39301865395916735
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	42438.0
3	6063.0
4	3765.0
5	3366.0
6	2834.0
7	3114.0
8	3664.0
9	3760.0
10	4061.0
11	4533.0
12	4997.0
13	5497.0
14	5946.0
15	6151.0
16	6113.0
17	6132.0
18	5919.0
19	5672.0
20	5826.0
21	6145.0
22	7109.0
23	10300.0
24	16639.0
25	22603.0
26	23905.0
27	19474.0
28	19781.0
29	24955.0
30	30188.0
31	36137.0
32	46955.0
33	63147.0
34	92030.0
35	144387.0
36	267067.0
37	569219.0
38	988118.0
39	487054.0
40	1070.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.90330226680462	20.157156953216447	13.450721667605453	23.48881911237348
2	15.1393598654507	20.17024401709032	41.20355975052983	23.486836366929158
3	18.135948536655118	25.828683896797656	29.899336466952022	26.136031099595208
4	12.07705150805422	16.82914933390596	39.63588301780634	31.45791614023348
5	12.63213509540094	38.51513311387389	35.84606411198824	13.006667678736935
6	30.438679195031266	38.67187083852685	18.079703197076714	12.809746769365166
7	27.028225520520703	32.92088511407827	22.701098053080614	17.349791312320413
8	24.677479977641408	37.30209433687766	20.409073007327134	17.6113526781538
9	24.62186823872979	17.637504198874684	21.34766149729291	36.39296606510262
10-11	23.438718512492798	27.595766082887607	29.057535803512263	19.907979601107336
12-13	24.038541404328367	24.96039645326665	29.109293471394647	21.891768671010336
14-15	21.350996719740827	27.04188934405969	26.095962270111787	25.511151666087695
16-17	21.053908860527727	30.461456517125583	26.644184875558125	21.84044974678856
18-19	20.9921336382672	29.38399030603243	28.65138335447231	20.972492701228056
20-21	22.232072235700798	28.674602455971215	28.06971434886935	21.023610959458633
22-23	21.85678563052543	28.390118764118128	28.188630396763138	21.564465208593308
24-25	21.665020194597187	28.46250579455524	28.186547643519795	21.68592636732778
26-27	21.486357960433608	28.391085227834495	28.272356756004584	21.850200055727313
28-29	21.410011845747956	28.490646652673252	28.368677225921235	21.730664275657563
30-31	21.683632535852038	28.9158932764823	27.98146659946456	21.419007588201104
32-33	21.357459420429286	28.607952360804056	28.412773605627063	21.621814613139595
34-35	21.50056607988101	28.37992631807065	28.50948720586233	21.610020396186012
36-37	21.751200901035702	28.78898611347131	27.941760231896524	21.51805275359646
38-39	21.618642751772903	28.816263024329057	28.14120577229043	21.42388845160761
40-41	21.401898201650262	28.516767181594492	28.419811458152495	21.66152315860275
42-43	21.36985074616928	28.445417135377117	28.405700895096857	21.779031223356746
44-45	21.49707194276818	28.602677256866215	28.325077356607093	21.575173443758512
46-47	21.489658074585442	28.748748208873337	28.224777074644038	21.536816641897182
48-49	21.417226729675384	28.5544323314097	28.274735147475262	21.753605791439654
50-51	21.45682104128372	28.44121733357615	28.282040670785975	21.819920954354153
52-53	21.493439322514455	28.410691651986948	28.330770278238248	21.76509874726035
54-55	21.56336215069293	28.818624760555895	28.125000520214016	21.493012568537157
56-57	21.467192406284173	28.535778986939686	28.17789672968628	21.81913187708986
58-59	21.34368468398284	28.252225780096722	28.481695368259984	21.922394167660453
60-61	21.65650845491144	28.605973744289386	28.06682696749289	21.67069083330628
62-63	21.41543173655934	28.584426771869808	28.156373154887493	21.843768336683354
64-65	21.37540784646579	28.354203742041783	28.351889955653537	21.91849845583889
66-67	21.27446295074676	28.418762337696712	28.187318424976233	22.119456286580295
68-69	21.766341968228836	28.503860909063516	28.07907685080856	21.650720271899086
70-71	21.687784825486805	28.256203032612216	28.199275387648964	21.85673675425201
72-73	21.405479311593815	28.39961808477772	28.375315599399165	21.819587004229298
74-75	21.53866666134013	28.47464968034122	28.177295770596718	21.809387887721936
76-77	21.489751665332538	28.511288673341813	28.063110772432324	21.93584888889333
78-79	21.609575684799655	28.48100075354137	28.17633833491493	21.733085226744045
80-81	21.581687770596098	28.293203408366445	28.271613980361444	21.853494840676014
82-83	21.420046521026844	28.386681816350805	28.375046565969875	21.818225096652476
84-85	21.476025226511275	28.26818415459761	28.212887972131522	22.042902646759597
86-87	21.646385716245135	28.39323071678445	28.11311923148115	21.847264335489267
88-89	21.396320368802034	28.2757712671045	28.19527558950267	22.132632774590796
90-91	21.40301001674451	28.141259732953394	28.316877332330215	22.138852917971885
92-93	21.546774372880723	28.3874294517862	28.097646650736657	21.968149524596416
94-95	21.35086797998973	28.157538846671432	28.295906316916934	22.195686856421904
96-97	21.42770591015919	28.116348977023815	28.292265594311544	22.16367951850545
98-99	21.450908305290177	28.152004609851993	28.197477297777198	22.19960978708063
100	21.493802978937584	28.145614016399335	28.065121983046744	22.29546102161634
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2706.0
1	1688.0
2	577.0
3	428.5
4	328.0
5	264.5
6	248.5
7	238.0
8	233.0
9	251.0
10	251.0
11	254.5
12	265.5
13	294.5
14	345.0
15	418.5
16	547.0
17	744.0
18	1083.5
19	1596.0
20	2320.0
21	3453.0
22	5306.5
23	8191.0
24	11885.5
25	16794.5
26	23236.0
27	30533.0
28	39224.5
29	48997.5
30	58495.0
31	67185.5
32	74909.0
33	82813.5
34	90448.0
35	96292.0
36	100570.5
37	104618.0
38	107550.0
39	107901.5
40	107708.0
41	107862.5
42	106839.0
43	104441.0
44	101381.0
45	99534.5
46	97805.5
47	94448.0
48	91618.0
49	89459.5
50	86552.0
51	82669.5
52	77498.0
53	72470.0
54	69146.5
55	66269.0
56	61504.0
57	56434.5
58	52069.0
59	46977.5
60	41463.0
61	35763.5
62	31144.5
63	26509.0
64	21588.0
65	17710.0
66	14450.0
67	11652.5
68	9229.0
69	7158.0
70	5572.0
71	4336.5
72	3402.5
73	2707.0
74	2159.5
75	1639.0
76	1212.0
77	974.0
78	749.5
79	534.0
80	383.5
81	248.0
82	180.0
83	130.0
84	91.0
85	68.5
86	58.0
87	45.5
88	35.5
89	30.0
90	25.0
91	20.0
92	11.0
93	5.0
94	4.5
95	4.5
96	6.0
97	5.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07511308544462755
2	0.0774416576240447
3	0.07867247434745091
4	0.08080145462577516
5	0.07950410726867133
6	0.07950410726867133
7	0.07860594371375328
8	0.07873900498114855
9	0.07827329054526512
10-11	0.07795727003520135
12-13	0.07649359609385344
14-15	0.07502992215250551
16-17	0.07389890137964575
18-19	0.07331675833479147
20-21	0.07285104389890804
22-23	0.07446441176607563
24-25	0.074397881132378
26-27	0.07388226872122135
28-29	0.06994032867463659
30-31	0.07517961607832518
32-33	0.07388226872122135
34-35	0.07122104337331603
36-37	0.06771155244576589
38-39	0.06960767550614842
40-41	0.07270134997308836
42-43	0.07734186167349826
44-45	0.07819012725314307
46-47	0.08110084247741452
48-49	0.0841113536522324
50-51	0.08492635391502841
52-53	0.08505941518242367
54-55	0.08524237442509215
56-57	0.08291380224567502
58-59	0.08209880198287901
60-61	0.0800529849966768
62-63	0.08055196474940904
64-65	0.07982012777873508
66-67	0.0792546173923052
68-69	0.07892196422381703
70-71	0.07720880040610299
72-73	0.07737512699034707
74-75	0.07670982065337073
76-77	0.07665992267809751
78-79	0.07709237179713213
80-81	0.07814022927786984
82-83	0.07754145357459115
84-85	0.076842881920766
86-87	0.07750818825774233
88-89	0.07471390164244175
90-91	0.0719861456608388
92-93	0.07185308439344354
94-95	0.07240196212144902
96-97	0.07165349249235065
98-99	0.0709549208385255
100	0.07018981855100272
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3006134.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.19525425000568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7349758849378	53.01156444591394
2	14.22262501327145	19.39831057765074
3	4.115646727563538	8.42002724968198
4	1.6063878122244297	4.381921011150215
5	0.7812274182432257	2.6638001207086153
6	0.44012489377037295	1.8008657419456398
7	0.2682819783685674	1.2806890407877283
8	0.16477760717269063	0.8989640652679358
9	0.11757844536855548	0.721646277860614
>10	0.5243651417119741	6.013116444277405
>50	0.02001922730830216	0.9131585003042384
>100	0.0038923209224384554	0.39710230379586053
>500	0.0	0.0
>1k	9.75291366436023E-5	0.0988342206550829
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.326531684881645E-5	0.0	0.0	0.0
3	0.0	3.326531684881645E-5	0.0	0.0	0.0
4	0.0	3.326531684881645E-5	0.0	6.65306336976329E-5	0.0
5	0.0	3.326531684881645E-5	0.0	6.65306336976329E-5	0.0
6	0.0	3.326531684881645E-5	0.0	6.65306336976329E-5	0.0
7	0.0	3.326531684881645E-5	0.0	6.65306336976329E-5	0.0
8	0.0	3.326531684881645E-5	0.0	6.65306336976329E-5	0.0
9	0.0	3.326531684881645E-5	0.0	6.65306336976329E-5	0.0
10-11	0.0	3.326531684881645E-5	0.0	8.316329212204113E-5	0.0
12-13	0.0	3.326531684881645E-5	0.0	9.979595054644936E-5	0.0
14-15	0.0	4.9897975273224674E-5	0.0	9.979595054644936E-5	0.0
16-17	0.0	6.65306336976329E-5	0.0	1.330612673952658E-4	0.0
18-19	0.0	6.65306336976329E-5	0.0	1.6632658424408226E-4	0.0
20-21	0.0	8.316329212204113E-5	0.0	2.1622455951730694E-4	0.0
22-23	0.0	9.979595054644936E-5	0.0	2.661225347905316E-4	0.0
24-25	0.0	1.330612673952658E-4	0.0	4.490817774590221E-4	0.0
26-27	0.0	1.6632658424408226E-4	0.0	5.987757032786961E-4	0.0
28-29	0.0	1.6632658424408226E-4	0.0	0.0014137759660746993	0.0
30-31	0.0	1.6632658424408226E-4	0.0	0.004573981066712262	0.0
32-33	0.0	1.6632658424408226E-4	0.0	0.011659493555510166	0.0
34-35	0.0	1.6632658424408226E-4	0.0	0.021688986585428328	0.0
36-37	0.0	1.829592426684905E-4	0.0	0.0408331764319222	0.0
38-39	0.0	2.1622455951730694E-4	0.0	0.07424818720655832	0.0
40-41	0.0	2.494898763661234E-4	0.0	0.11516452693060256	0.0
42-43	0.0	2.661225347905316E-4	0.0	0.15855913275988362	0.0
44-45	0.0	2.661225347905316E-4	0.0	0.20730945460182415	0.0
46-47	0.0	2.8275519321493986E-4	0.0	0.2672701882218158	0.0
48-49	0.0	2.993878516393481E-4	0.0	0.336462047267354	0.0
50-51	0.0	2.993878516393481E-4	0.0	0.4157332973180836	0.0
52-53	0.0	3.3265316848816453E-4	0.0	0.49576964965633596	0.0
54-55	0.0	3.3265316848816453E-4	0.0	0.5817272283936777	0.0
56-57	0.0	3.3265316848816453E-4	0.0	0.6850992005013748	0.0
58-59	0.0	3.3265316848816453E-4	0.0	0.8061683211726424	0.0
60-61	0.0	3.825511437613892E-4	0.0	0.936618261195276	0.0
62-63	0.0	3.9918380218579745E-4	0.0	1.0703115696106695	0.0
64-65	0.0	3.9918380218579745E-4	0.0	1.2087950836522923	0.0
66-67	0.0	3.9918380218579745E-4	0.0	1.368701461744553	0.0
68-69	0.0	3.9918380218579745E-4	0.0	1.5558853996528432	0.0
70-71	1.6632658424408226E-5	3.9918380218579745E-4	0.0	1.7560095458153229	0.0
72-73	3.326531684881645E-5	3.9918380218579745E-4	0.0	1.9690905328904167	0.0
74-75	3.326531684881645E-5	3.9918380218579745E-4	0.0	2.181323254385866	0.0
76-77	3.326531684881645E-5	3.9918380218579745E-4	0.0	2.403668632203355	0.0
78-79	3.326531684881645E-5	3.9918380218579745E-4	0.0	2.664468716298076	0.0
80-81	3.326531684881645E-5	4.1581646061020563E-4	0.0	2.947689623948899	0.0
82-83	3.326531684881645E-5	4.324491190346139E-4	0.0	3.247543190024131	0.0
84-85	3.326531684881645E-5	4.324491190346139E-4	0.0	3.5435878773201726	0.0
86-87	3.326531684881645E-5	4.324491190346139E-4	0.0	3.8555001207531	0.0
88	3.326531684881645E-5	4.324491190346139E-4	0.0	4.114021530643678	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3965	0.0	35.563	1
TCAACGC	3920	0.0	34.172684	4
GGTATCA	2380	0.0	34.165634	1
ATCAACG	3975	0.0	33.699856	3
CAACGCA	4095	0.0	32.710136	5
AACGCAG	4305	0.0	31.114	6
TATCAAC	4930	0.0	27.743828	2
GTACATG	8810	0.0	27.742638	1
TACATGG	8625	0.0	26.811821	2
ACGCAGA	5175	0.0	25.337908	7
ACATGGG	8970	0.0	24.627806	3
CGCAGAG	5355	0.0	24.486217	8
CATGGGG	5490	0.0	20.54751	4
TAGACCG	120	0.0050664335	19.583042	5
GCAGAGT	6620	0.0	19.097267	9
GAGTACA	6440	0.0	17.73539	1
CCGATCG	195	2.469349E-4	16.870983	9
GACGCGT	85	0.0019205273	16.587437	10-11
AGAGTAC	5620	0.0	16.181604	10-11
GTCTACG	320	1.8097126E-7	16.157085	1
>>END_MODULE