FastQCFastQC Report
Wed 25 May 2016
SRR1032916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3006134
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68980.22946415562313588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55110.1833251611538275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48200.052.051751
GTATCAA86800.040.9748421
TCAACGC103250.033.895694
ATCAACG103550.033.8429153
CAACGCA105550.033.109765
AACGCAG108900.032.2208066
TATCAAC109550.032.0757642
ACGCAGA126450.027.6363687
CGCAGAG128150.027.269758
GTACATG89000.025.18081
GCAGAGT141200.024.5201639
TACATGG89150.024.219222
ACATGGG86200.023.737973
GTGGTAT25400.022.9366021
TGGTATC25150.022.4446132
TAGACCG1703.432373E-622.1343355
GAGTACT97700.021.39715412-13
AGAGTAC130250.020.81079310-11
CAGAGTA141850.020.51646410-11
GTACTTT109200.019.10076914-15
Produced by FastQC (version 0.11.5)