##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032916_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3006134 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.81140095551296 34.0 31.0 34.0 31.0 34.0 2 32.87606806616073 34.0 31.0 34.0 31.0 34.0 3 32.94941077144266 34.0 31.0 34.0 31.0 34.0 4 36.36057807137007 37.0 37.0 37.0 35.0 37.0 5 36.34960018415679 37.0 37.0 37.0 35.0 37.0 6 36.40998904240463 37.0 37.0 37.0 35.0 37.0 7 36.374183918614406 37.0 37.0 37.0 35.0 37.0 8 36.31490545664298 37.0 37.0 37.0 35.0 37.0 9 38.15019756271676 39.0 39.0 39.0 37.0 39.0 10-11 38.161906122614624 39.0 39.0 39.0 37.0 39.0 12-13 38.159606491260874 39.0 39.0 39.0 37.0 39.0 14-15 39.70460232311667 41.0 40.0 41.0 37.0 41.0 16-17 39.71676761581486 41.0 40.0 41.0 37.0 41.0 18-19 39.74717244806785 41.0 40.0 41.0 37.0 41.0 20-21 39.76509580078599 41.0 40.0 41.0 37.0 41.0 22-23 39.72428391415686 41.0 40.0 41.0 37.0 41.0 24-25 39.70410816683488 41.0 40.0 41.0 37.0 41.0 26-27 39.626455274448844 41.0 40.0 41.0 37.0 41.0 28-29 39.56188962301747 41.0 40.0 41.0 37.0 41.0 30-31 39.44741069426712 41.0 39.5 41.0 36.0 41.0 32-33 39.31257039772678 41.0 39.0 41.0 35.5 41.0 34-35 39.21426024255739 41.0 39.0 41.0 35.0 41.0 36-37 39.12018160201774 41.0 39.0 41.0 35.0 41.0 38-39 39.01621551135112 41.0 39.0 41.0 35.0 41.0 40-41 38.90781764884732 41.0 39.0 41.0 35.0 41.0 42-43 38.76668288905285 41.0 39.0 41.0 35.0 41.0 44-45 38.59045455059555 41.0 39.0 41.0 35.0 41.0 46-47 38.35578753309068 41.0 38.5 41.0 35.0 41.0 48-49 38.14451168843438 41.0 38.0 41.0 34.0 41.0 50-51 37.99098110729595 40.5 38.0 41.0 34.0 41.0 52-53 37.85334070270986 40.0 38.0 41.0 34.0 41.0 54-55 37.71592567064542 40.0 38.0 41.0 33.5 41.0 56-57 37.515742811198706 40.0 37.0 41.0 33.0 41.0 58-59 37.35825149510967 40.0 37.0 41.0 33.0 41.0 60-61 37.18317912641286 40.0 36.0 41.0 33.0 41.0 62-63 36.96395869246015 39.5 36.0 41.0 32.5 41.0 64-65 36.69106832895673 39.0 35.0 41.0 32.0 41.0 66-67 36.43379453477456 39.0 35.0 41.0 32.0 41.0 68-69 36.15356018727043 38.5 35.0 40.5 31.5 41.0 70-71 35.84118006715602 37.0 35.0 40.0 31.0 41.0 72-73 35.50517026187123 37.0 35.0 39.5 31.0 41.0 74-75 35.16341952820466 36.5 35.0 39.0 31.0 41.0 76-77 34.81087286860799 36.0 35.0 39.0 31.0 40.5 78-79 34.46352541170819 35.5 35.0 38.0 30.0 39.5 80-81 34.12148061264068 35.0 34.5 37.0 30.0 39.0 82-83 33.79624644144273 35.0 34.0 37.0 30.0 39.0 84-85 33.5015243831446 35.0 34.0 36.0 30.0 38.0 86-87 33.34162249587011 35.0 34.0 36.0 30.0 37.0 88-89 33.13719298607447 35.0 34.0 36.0 30.0 37.0 90-91 32.94974092971238 35.0 34.0 35.5 29.5 36.5 92-93 32.777456859873844 35.0 34.0 35.0 29.0 36.0 94-95 32.673180902780786 35.0 34.0 35.0 29.5 36.0 96-97 32.605408142152015 35.0 34.0 35.0 29.5 36.0 98-99 32.492431641437136 35.0 34.0 35.0 29.0 36.0 100 32.4072067313034 35.0 34.0 35.0 29.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2566797912133296 1101 2 0.27261779975012246 1101 3 0.258984410728452 1101 4 0.13773635951064023 1101 5 0.16579698199916493 1101 6 0.16089386613632684 1101 7 0.1714040203893461 1101 8 0.10550248656250005 1101 9 0.17343215802979728 1101 10-11 0.2467608298044155 1101 12-13 0.26718055862357915 1101 14-15 0.3428473957933278 1101 16-17 0.32635809581639563 1101 18-19 0.35045401328287085 1101 20-21 0.38324517725315843 1101 22-23 0.3393515854918121 1101 24-25 0.35636814750744605 1101 26-27 0.38948816688323973 1101 28-29 0.3406695183168722 1101 30-31 0.2955229886370887 1101 32-33 0.3640297456963637 1101 34-35 0.35954228350769313 1101 36-37 0.3671278719952227 1101 38-39 0.39910852645117245 1101 40-41 0.40324494295038704 1101 42-43 0.46597905389634775 1101 44-45 0.3811146937118437 1101 46-47 0.44654373447539797 1101 48-49 0.35534396943052826 1101 50-51 0.35276939800134954 1101 52-53 0.432436175250281 1101 54-55 0.43245589957788155 1101 56-57 0.43411240652923766 1101 58-59 0.46360046195867 1101 60-61 0.43720431798728754 1101 62-63 0.4829959107837567 1101 64-65 0.4883830944235399 1101 66-67 0.4314760915763145 1101 68-69 0.40751074610856364 1101 70-71 0.4241851100918055 1101 72-73 0.43253264610331144 1101 74-75 0.5343244212545173 1101 76-77 0.45717771817386677 1101 78-79 0.4449573564173548 1101 80-81 0.4757800885436012 1101 82-83 0.3768300521550927 1101 84-85 0.421844845332906 1101 86-87 0.40261556678417065 1101 88-89 0.3546587588209249 1101 90-91 0.3845395020677813 1101 92-93 0.42427084215072597 1101 94-95 0.4379712577295436 1101 96-97 0.4133946263944992 1101 98-99 0.3741085600135392 1101 100 0.35104290758310697 1102 1 0.20947954810657876 1102 2 0.2022554743285383 1102 3 0.2094348422472052 1102 4 0.13153626021403397 1102 5 0.1729918747107746 1102 6 0.11744267609672931 1102 7 0.15674909072566123 1102 8 1.6968249312299122E-6 1102 9 0.16006122574476223 1102 10-11 0.15988247899305463 1102 12-13 0.2102236409384659 1102 14-15 0.24621754781240668 1102 16-17 0.253807736531634 1102 18-19 0.2550149388671272 1102 20-21 0.27129181340234254 1102 22-23 0.25979543073748346 1102 24-25 0.20962436303238263 1102 26-27 0.2117548498496049 1102 28-29 0.22410966159343815 1102 30-31 0.17742711654856436 1102 32-33 0.1923376728756807 1102 34-35 0.32334912011153705 1102 36-37 0.28562656476042037 1102 38-39 0.2432423913399333 1102 40-41 0.23503580438532623 1102 42-43 0.18352679676235084 1102 44-45 0.23472001886559468 1102 46-47 0.2632247242911916 1102 48-49 0.2565771002906345 1102 50-51 0.2695982546120348 1102 52-53 0.2578170086900542 1102 54-55 0.349268127569772 1102 56-57 0.31260389112318876 1102 58-59 0.2331326782021037 1102 60-61 0.2913956344494508 1102 62-63 0.33566957645435735 1102 64-65 0.37083148981969316 1102 66-67 0.4360709809621852 1102 68-69 0.4189055223711122 1102 70-71 0.4080262649974955 1102 72-73 0.3887214434210904 1102 74-75 0.3901053544903661 1102 76-77 0.3835565489310184 1102 78-79 0.39100364038093005 1102 80-81 0.41698059198265724 1102 82-83 0.3597116504808682 1102 84-85 0.39041300978738747 1102 86-87 0.28899178812729076 1102 88-89 0.2950723874907055 1102 90-91 0.29079602902907453 1102 92-93 0.28200706949720455 1102 94-95 0.23526339984222489 1102 96-97 0.283380450649247 1102 98-99 0.3576930981755808 1102 100 0.3603255142488422 1103 1 0.20438545867440894 1103 2 0.23784813146799877 1103 3 0.22644172569903276 1103 4 0.12337176708835074 1103 5 0.13255754347878224 1103 6 0.14149787541642667 1103 7 0.13668797584684 1103 8 0.12559505321130615 1103 9 0.1444623766051336 1103 10-11 0.1707161523512042 1103 12-13 0.1738344124125888 1103 14-15 0.22944969976748553 1103 16-17 0.25403618433959707 1103 18-19 0.2356749732756569 1103 20-21 0.2414966811149526 1103 22-23 0.2535826334950002 1103 24-25 0.22215440825306132 1103 26-27 0.22746501421219278 1103 28-29 0.24275549954430886 1103 30-31 0.2618743583685088 1103 32-33 0.3076805173632806 1103 34-35 0.3081067298319269 1103 36-37 0.23404209567440404 1103 38-39 0.2730820846206825 1103 40-41 0.2710121747260388 1103 42-43 0.25415489501666855 1103 44-45 0.2906961018286225 1103 46-47 0.28372705699520395 1103 48-49 0.2813823611871058 1103 50-51 0.3168123722793581 1103 52-53 0.3272875682423262 1103 54-55 0.3257942863272092 1103 56-57 0.2604356788415956 1103 58-59 0.2790572030487013 1103 60-61 0.2944596099578902 1103 62-63 0.30181869568829 1103 64-65 0.36913197658164165 1103 66-67 0.3451836866510263 1103 68-69 0.35541617087783806 1103 70-71 0.3818500554719364 1103 72-73 0.3684569826515798 1103 74-75 0.31604268567779314 1103 76-77 0.3372294917169114 1103 78-79 0.31003727532121417 1103 80-81 0.3392140960090231 1103 82-83 0.31720098638361804 1103 84-85 0.34535954764835 1103 86-87 0.36416406057855255 1103 88-89 0.3677995935827383 1103 90-91 0.3507069175136195 1103 92-93 0.3576715780388966 1103 94-95 0.29480412957910573 1103 96-97 0.2960162555121144 1103 98-99 0.3188082795010132 1103 100 0.34146911687833637 1104 1 0.11309625383770339 1104 2 0.1692980299417286 1104 3 0.17461266335673287 1104 4 0.09458115492400765 1104 5 0.09914692302280059 1104 6 0.08876812338424855 1104 7 0.10694468565389315 1104 8 0.11374999038157796 1104 9 0.1540951203840848 1104 10-11 0.1533452589332569 1104 12-13 0.16040966569845239 1104 14-15 0.19419365885498507 1104 16-17 0.16775491351943117 1104 18-19 0.1680017196533612 1104 20-21 0.1713071256715466 1104 22-23 0.17895900257644826 1104 24-25 0.17580415519899617 1104 26-27 0.16688816882484758 1104 28-29 0.18731821469986443 1104 30-31 0.19640862232622425 1104 32-33 0.2143139313613318 1104 34-35 0.22033733420795443 1104 36-37 0.17576333353992624 1104 38-39 0.1746847162240286 1104 40-41 0.17903089582615195 1104 42-43 0.2747624764396761 1104 44-45 0.2717660549530265 1104 46-47 0.26879663527374476 1104 48-49 0.18877308302366913 1104 50-51 0.1428431652704063 1104 52-53 0.10683581938110365 1104 54-55 0.15113168071140848 1104 56-57 0.1903080458143691 1104 58-59 0.18954470796607126 1104 60-61 0.09843343962422324 1104 62-63 0.12438829052362621 1104 64-65 0.125294084831296 1104 66-67 0.05289564343911479 1104 68-69 0.08721462301610927 1104 70-71 0.06146960017330372 1104 72-73 0.0899079341375213 1104 74-75 0.11965839185161542 1104 76-77 0.10085755588310974 1104 78-79 0.08573838939489065 1104 80-81 0.10279381433156942 1104 82-83 0.09822477916141281 1104 84-85 0.05476062874088683 1104 86-87 0.0730901272004516 1104 88-89 0.0664637581864369 1104 90-91 0.08748019007688868 1104 92-93 0.12925750052395557 1104 94-95 0.03963294555628494 1104 96-97 0.042413496752580215 1104 98-99 0.046838073786133805 1104 100 0.04844628307015597 1105 1 0.09199813913057397 1105 2 0.11979479807426685 1105 3 0.09367461627527263 1105 4 0.07674681253369187 1105 5 0.0753163624639015 1105 6 0.09188137426194487 1105 7 0.07732139253752734 1105 8 0.020445177675732396 1105 9 0.06650922734391429 1105 10-11 0.08320850712589589 1105 12-13 0.104584875601887 1105 14-15 0.14602574537169488 1105 16-17 0.14133324142261472 1105 18-19 0.13994523941848058 1105 20-21 0.17260806472736334 1105 22-23 0.17159979935134118 1105 24-25 0.1815769603816051 1105 26-27 0.15033689139851703 1105 28-29 0.19822707041471688 1105 30-31 0.20940735160727542 1105 32-33 0.20770159666903965 1105 34-35 0.2553326737820072 1105 36-37 0.22377638210080875 1105 38-39 0.1915468554332449 1105 40-41 0.17443201571828837 1105 42-43 0.14292591271303934 1105 44-45 0.12462474085611319 1105 46-47 0.16646688033886647 1105 48-49 0.2697016270901784 1105 50-51 0.22345331884964992 1105 52-53 0.19715747511483528 1105 54-55 0.210833042806712 1105 56-57 0.2849425961864469 1105 58-59 0.29298076125707695 1105 60-61 0.3219054969695776 1105 62-63 0.3031847792201461 1105 64-65 0.3100825535096803 1105 66-67 0.3360948605380969 1105 68-69 0.2528259022156405 1105 70-71 0.22066260624912104 1105 72-73 0.3088955950648966 1105 74-75 0.2660162731836522 1105 76-77 0.274532577621585 1105 78-79 0.21766103306310214 1105 80-81 0.2275025113855591 1105 82-83 0.25197355798450616 1105 84-85 0.25997441972678104 1105 86-87 0.2418693325188528 1105 88-89 0.22552172141813998 1105 90-91 0.21084351292478942 1105 92-93 0.2186642607318774 1105 94-95 0.20099795651870522 1105 96-97 0.23100396113780164 1105 98-99 0.18816980787524074 1105 100 0.14688161823859502 1106 1 0.08526488092047657 1106 2 0.1176750177435153 1106 3 0.08236985405498842 1106 4 0.029445110208477843 1106 5 0.08100072366483602 1106 6 0.08055717414273289 1106 7 0.0654239992597141 1106 8 0.05900117463982468 1106 9 0.08985889101079891 1106 10-11 0.11831375235547625 1106 12-13 0.07042958897557838 1106 14-15 0.12077056798238317 1106 16-17 0.11592485941083908 1106 18-19 0.10379157188420152 1106 20-21 0.09911754125275252 1106 22-23 0.10601757756351304 1106 24-25 0.0779328590169115 1106 26-27 0.10542387699562283 1106 28-29 0.10612625777562812 1106 30-31 0.1077271026462725 1106 32-33 0.1639407649748108 1106 34-35 0.10995300489486937 1106 36-37 0.14720834952947826 1106 38-39 0.16802240058261475 1106 40-41 0.14612496327379176 1106 42-43 0.11532409622388684 1106 44-45 0.1235329455532721 1106 46-47 0.17582852724558506 1106 48-49 0.1379026785256059 1106 50-51 0.1530228153595985 1106 52-53 0.23460791034638362 1106 54-55 0.2623178659300791 1106 56-57 0.24817790904243253 1106 58-59 0.1766486588074585 1106 60-61 0.15309741344197647 1106 62-63 0.14876223997195837 1106 64-65 0.0628008737194321 1106 66-67 0.07890882170656255 1106 68-69 0.1555162073661691 1106 70-71 0.1899343111492371 1106 72-73 0.18867643750143515 1106 74-75 0.1436374351292926 1106 76-77 0.1593448627334837 1106 78-79 0.12977901901094668 1106 80-81 0.13301525942426906 1106 82-83 0.12749999899865117 1106 84-85 0.1397401715579818 1106 86-87 0.14801083912391988 1106 88-89 0.1285095163663854 1106 90-91 0.07924998647467874 1106 92-93 0.05758364147139616 1106 94-95 0.13092508038774042 1106 96-97 0.10116706609681358 1106 98-99 0.09622175013858936 1106 100 0.08673561213469583 1107 1 0.05657510791223075 1107 2 0.07225566361502445 1107 3 0.08081853876123546 1107 4 0.05168540938202426 1107 5 0.0506453507907807 1107 6 0.031250406395777475 1107 7 0.014845037093991209 1107 8 0.08174235600625224 1107 9 0.07340002961828418 1107 10-11 0.0840038291086529 1107 12-13 0.08482097148430512 1107 14-15 0.11031961322820649 1107 16-17 0.13422487567719799 1107 18-19 0.12322103548977736 1107 20-21 0.08319772933415948 1107 22-23 0.11361438738718022 1107 24-25 0.14098651091595826 1107 26-27 0.15045916833221185 1107 28-29 0.15555773910501358 1107 30-31 0.17178179469424748 1107 32-33 0.12133789546157203 1107 34-35 0.16129828205233565 1107 36-37 0.11658051875929232 1107 38-39 0.15536665164133723 1107 40-41 0.1947698509801441 1107 42-43 0.2068112861400806 1107 44-45 0.21373418055089388 1107 46-47 0.17872576426758258 1107 48-49 0.2230078934798172 1107 50-51 0.23263360758923568 1107 52-53 0.21021076146399764 1107 54-55 0.2696223013447323 1107 56-57 0.33568563483883906 1107 58-59 0.318303444368901 1107 60-61 0.31354078543652264 1107 62-63 0.2874541002307467 1107 64-65 0.26605555058741004 1107 66-67 0.18720228942349593 1107 68-69 0.2370854808364058 1107 70-71 0.2451096423680852 1107 72-73 0.2332471309103994 1107 74-75 0.2129126435320572 1107 76-77 0.22907344339296998 1107 78-79 0.26944367418224147 1107 80-81 0.2699625055788957 1107 82-83 0.310906168830158 1107 84-85 0.3028654649644196 1107 86-87 0.22164537386476013 1107 88-89 0.2592543982933364 1107 90-91 0.22196867594205827 1107 92-93 0.23203549196168183 1107 94-95 0.30609689471253887 1107 96-97 0.3150667931898923 1107 98-99 0.2704263461744887 1107 100 0.24806602940085298 1108 1 0.05304784006033714 1108 2 0.07745683283562244 1108 3 0.09885948092924934 1108 4 0.03923864586870707 1108 5 0.030177492389732663 1108 6 0.02820693236254357 1108 7 0.06393101317852512 1108 8 0.08794416361591573 1108 9 0.08199158889571834 1108 10-11 0.0648139408996613 1108 12-13 0.07710455590985532 1108 14-15 0.06470571912225864 1108 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50-51 -0.21388411701266818 2214 52-53 -0.2271547378971377 2214 54-55 -0.18913075337348317 2214 56-57 -0.2184566044195151 2214 58-59 -0.1891912111663956 2214 60-61 -0.22565680056077753 2214 62-63 -0.213689966689806 2214 64-65 -0.30461437858033946 2214 66-67 -0.24445286131728494 2214 68-69 -0.2492860514640185 2214 70-71 -0.28550049495467533 2214 72-73 -0.20464752214390103 2214 74-75 -0.2771274037721412 2214 76-77 -0.21965653812365815 2214 78-79 -0.18070797629597024 2214 80-81 -0.25174491133896737 2214 82-83 -0.24284221335799572 2214 84-85 -0.24944392268603366 2214 86-87 -0.22610689107182935 2214 88-89 -0.24852546765053773 2214 90-91 -0.21425226030064692 2214 92-93 -0.17249413311946427 2214 94-95 -0.17775699155063762 2214 96-97 -0.19680769769040296 2214 98-99 -0.21523634677411962 2214 100 -0.1952873585969499 2215 1 -0.22979984016463106 2215 2 -0.26802315543562116 2215 3 -0.26537667728898384 2215 4 -0.15389214387825945 2215 5 -0.2128156125870504 2215 6 -0.1772726995444387 2215 7 -0.20379853854650065 2215 8 -0.14889274878162695 2215 9 -0.19460744723274104 2215 10-11 -0.2507074770612192 2215 12-13 -0.2435591748675776 2215 14-15 -0.3092279819644119 2215 16-17 -0.32247399045844816 2215 18-19 -0.3203695150237138 2215 20-21 -0.3190812561595564 2215 22-23 -0.3020310289627872 2215 24-25 -0.3193567199191918 2215 26-27 -0.3981760526366358 2215 28-29 -0.37443045772833017 2215 30-31 -0.4226582317423393 2215 32-33 -0.355943387356092 2215 34-35 -0.3586812100409489 2215 36-37 -0.33363680266576523 2215 38-39 -0.33298761212967065 2215 40-41 -0.34101177730926935 2215 42-43 -0.3431575870776271 2215 44-45 -0.2783631856781099 2215 46-47 -0.22898855518035077 2215 48-49 -0.26674798880357997 2215 50-51 -0.19631679699068627 2215 52-53 -0.20685156881856415 2215 54-55 -0.18046982646379917 2215 56-57 -0.30077845823889504 2215 58-59 -0.2570607843482904 2215 60-61 -0.22292644701617093 2215 62-63 -0.23996217771746586 2215 64-65 -0.28385141833120997 2215 66-67 -0.3093823355799685 2215 68-69 -0.24919354367870739 2215 70-71 -0.2978241806113928 2215 72-73 -0.2894789931092845 2215 74-75 -0.30242599325739405 2215 76-77 -0.2805266608567578 2215 78-79 -0.31897047858576855 2215 80-81 -0.3079456807106524 2215 82-83 -0.32209573964485827 2215 84-85 -0.39545051730043923 2215 86-87 -0.4649308515041426 2215 88-89 -0.5090173159744111 2215 90-91 -0.4946085526618802 2215 92-93 -0.47140602174375346 2215 94-95 -0.4837764090263619 2215 96-97 -0.5107085952906942 2215 98-99 -0.5375334707895121 2215 100 -0.5278867357722561 2216 1 -0.3554893233829688 2216 2 -0.3628373435933696 2216 3 -0.34695835055411095 2216 4 -0.22691530671869486 2216 5 -0.26163586533659355 2216 6 -0.25710525695988906 2216 7 -0.23201980111699783 2216 8 -0.2044188051549085 2216 9 -0.32297822733689685 2216 10-11 -0.35512316771065855 2216 12-13 -0.3780934962378524 2216 14-15 -0.48464813558569375 2216 16-17 -0.4349084218214827 2216 18-19 -0.4307852637088274 2216 20-21 -0.4225691322019287 2216 22-23 -0.47094150726486816 2216 24-25 -0.5030606427465827 2216 26-27 -0.521899766045955 2216 28-29 -0.49139653765694646 2216 30-31 -0.5120164636232403 2216 32-33 -0.6441692907186933 2216 34-35 -0.6409766259917973 2216 36-37 -0.5927623300066287 2216 38-39 -0.5665114764867951 2216 40-41 -0.5468010468407485 2216 42-43 -0.6037557527923383 2216 44-45 -0.6191306739485825 2216 46-47 -0.6992914911535166 2216 48-49 -0.7480974574332251 2216 50-51 -0.6872328194887345 2216 52-53 -0.6453602311262188 2216 54-55 -0.7017792037106148 2216 56-57 -0.6899600182106624 2216 58-59 -0.7417419930803888 2216 60-61 -0.8063827623716406 2216 62-63 -0.7154620925892914 2216 64-65 -0.7455433263960884 2216 66-67 -0.7371071901744415 2216 68-69 -0.6970490975289536 2216 70-71 -0.6925097447705255 2216 72-73 -0.6554511087493751 2216 74-75 -0.7438782820088647 2216 76-77 -0.6735988294716151 2216 78-79 -0.644650338469468 2216 80-81 -0.6825547305921873 2216 82-83 -0.6639072170409221 2216 84-85 -0.6218704765984384 2216 86-87 -0.588428198228037 2216 88-89 -0.5668292667326398 2216 90-91 -0.5661076770381399 2216 92-93 -0.6248896654442433 2216 94-95 -0.7067071445338087 2216 96-97 -0.5639175906021876 2216 98-99 -0.5899049540606534 2216 100 -0.5485019883210143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14.0 3 6.0 4 5.0 5 20.0 6 60.0 7 192.0 8 395.0 9 714.0 10 1282.0 11 2140.0 12 3509.0 13 5354.0 14 6496.0 15 8564.0 16 10583.0 17 15153.0 18 18491.0 19 10073.0 20 6561.0 21 5837.0 22 6191.0 23 6345.0 24 7131.0 25 8311.0 26 10500.0 27 13451.0 28 16967.0 29 21858.0 30 27732.0 31 35464.0 32 45466.0 33 59240.0 34 82880.0 35 128384.0 36 237968.0 37 541604.0 38 1053381.0 39 605196.0 40 2616.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7954260052393 20.24177304669633 13.757445216017297 23.20535573204707 2 15.448014234955945 20.40356991618102 40.66243507829525 23.485980770567785 3 18.259913257128943 25.86734554705957 30.317957397660646 25.554783798150844 4 12.11704233552358 17.060101472178324 39.29618231722532 31.526673875072774 5 12.284959730523335 38.13230529821966 35.96615973464825 13.61657523660875 6 30.23267263822735 38.643478133589 17.728688973415398 13.395160254768253 7 26.638976432696833 33.05762855300897 22.703197550059645 17.600197464234558 8 24.95223165449029 36.352577025624356 20.380494525627686 18.314696794257674 9 24.9626259192161 16.46821423415236 21.677033386181538 36.89212646045 10-11 23.582481685779808 26.991029009352214 29.014225580097232 20.412263724770753 12-13 24.604699003069396 24.321859987951274 29.442851216864756 21.63058979211457 14-15 21.659673174262707 26.207550044442552 27.058428649744787 25.074348131549957 16-17 20.66634421486201 29.825882678549924 27.494416416566924 22.013356690021137 18-19 20.48479274016759 28.209292345973065 30.50051062329307 20.805404290566276 20-21 21.578080019054376 27.473359471001622 30.225615358463724 20.722945151480275 22-23 21.203278168821402 27.246198142827843 30.409884204921976 21.140639483428775 24-25 21.009693726413737 27.430346664528805 30.33435630220289 21.225603306854566 26-27 20.83978585018362 27.373165004010403 30.42275060688312 21.364298538922856 28-29 20.836617162764718 27.451960167550986 30.542374956377326 21.16904771330697 30-31 21.029985088302404 27.846297726914315 30.127717592010367 20.995999592772918 32-33 20.797960473782272 27.556261644929464 30.498752328985894 21.147025552302367 34-35 20.94520357416893 27.389162364882267 30.490173830465885 21.17546023048292 36-37 21.224239396965157 27.75744962438442 29.928613988583365 21.089696990067054 38-39 21.11177374422059 27.890704584958904 29.957215791685897 21.04030587913461 40-41 20.986133988519377 27.599623370092136 30.128578708028947 21.28566393335954 42-43 21.083454198708335 27.602727801410836 29.889055738772335 21.42476226110849 44-45 21.188619089356646 27.795425603618916 29.73589091567851 21.28006439134593 46-47 21.29152183708683 28.052211583901908 29.333589105951475 21.322677473059784 48-49 21.29560233724545 27.976193945214835 29.162333855241112 21.565869862298605 50-51 21.346614264956784 28.00668464825502 28.969784204288075 21.676916882500123 52-53 21.41621774808687 28.0269888967684 28.89281217983695 21.66398117530778 54-55 21.479696157137454 28.371910937280663 28.737318996539912 21.411073909041967 56-57 21.32518026647922 28.07287146199615 28.851111184738155 21.750837086786472 58-59 21.171479756455163 27.795662200989913 29.16278424759759 21.87007379495733 60-61 21.423299912181033 28.057352861608127 28.83773468682757 21.68161253938327 62-63 21.212575249054392 28.064900644611367 28.87547613339726 21.847047972936977 64-65 21.202780714366025 27.812283151715793 29.082835296097915 21.90210083782027 66-67 21.132208344671263 27.906889712833827 28.93435555434322 22.026546388151694 68-69 21.5162267410945 28.002049809165165 28.817216534129308 21.664506915611035 70-71 21.4747246796051 27.8204664196606 28.85757920305615 21.84722969767815 72-73 21.299749112980326 27.944279928971895 28.907344117061978 21.848626840985798 74-75 21.35824617265897 28.019060361248034 28.73211906056084 21.890574405532156 76-77 21.36764717867653 27.996511132988605 28.642357367029774 21.993484321305093 78-79 21.42434792067618 28.055107998364008 28.723657814930707 21.7968862660291 80-81 21.368875579794164 27.89066280034656 28.790490369433275 21.949971250426003 82-83 21.13429797256109 27.96796215735667 28.909420991922346 21.988318878159895 84-85 21.200917856622493 27.829431422551355 28.75959621227796 22.21005450854819 86-87 21.282123485316344 27.973418843493818 28.64262156348336 22.101836107706475 88-89 20.97374688967839 27.8777294319588 28.730855720995834 22.417667957366973 90-91 20.951079485378468 27.754890296715228 28.850767173691505 22.4432630442148 92-93 21.012862621454577 28.0008722225558 28.61921214884268 22.367053007146936 94-95 20.775250943037523 27.770951866207184 28.80996889502232 22.643828295732973 96-97 20.807961590521774 27.621282890677158 28.848573898759632 22.72218162004143 98-99 20.748662692458602 27.730837635786965 28.7385939446801 22.78190572707433 100 20.829078144886424 27.684827090209552 28.6194494323939 22.866645332510128 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 67.0 1 60.0 2 192.0 3 337.0 4 221.0 5 147.5 6 384.5 7 889.0 8 1623.5 9 1979.5 10 1579.5 11 1234.5 12 1311.0 13 1531.0 14 1798.5 15 2084.0 16 2375.0 17 2750.5 18 3185.0 19 3600.5 20 4302.0 21 5439.5 22 7117.5 23 9817.5 24 13485.5 25 18100.5 26 24041.0 27 30890.5 28 39368.0 29 49075.5 30 58013.0 31 66711.0 32 74699.0 33 82147.5 34 89254.5 35 94693.0 36 99410.5 37 103547.0 38 106435.0 39 107508.0 40 107555.0 41 107256.0 42 105972.5 43 104145.0 44 101358.0 45 99012.5 46 97505.5 47 94601.5 48 91706.5 49 88928.5 50 85896.5 51 82191.0 52 76521.5 53 71789.5 54 68472.5 55 65085.0 56 60667.5 57 55721.5 58 51178.5 59 46437.0 60 41214.5 61 35547.5 62 30620.0 63 25926.0 64 21016.0 65 17197.0 66 14054.5 67 11083.0 68 8691.0 69 6694.0 70 5009.0 71 3800.5 72 3041.5 73 2377.0 74 1843.0 75 1349.0 76 968.0 77 777.0 78 573.5 79 398.0 80 263.0 81 143.5 82 73.5 83 43.5 84 25.0 85 14.0 86 6.5 87 2.5 88 1.5 89 1.0 90 2.0 91 2.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.993878516393481E-4 2 4.324491190346139E-4 3 2.661225347905316E-4 4 0.012773881669945518 5 3.65918485336981E-4 6 0.0035926542196721774 7 6.653063369763291E-4 8 0.13791800365519302 9 0.0013306126739526581 10-11 0.0 12-13 2.3285721794171518E-4 14-15 1.9959190109289872E-4 16-17 0.0 18-19 1.330612673952658E-4 20-21 0.0 22-23 1.4969392581967405E-4 24-25 4.823470943078386E-4 26-27 0.006686328686612107 28-29 0.008083471994262398 30-31 0.01463673941347924 32-33 0.017763679197267984 34-35 0.02634613094426263 36-37 0.03770623664813345 38-39 0.042812462784426776 40-41 0.05299164974016461 42-43 0.069956961333061 44-45 0.1079792184912582 46-47 0.11533085351484663 48-49 0.11205421980523822 50-51 0.11419983274198688 52-53 0.1087609534372054 54-55 0.11521442490587579 56-57 0.10014523637336192 58-59 0.09129666209157676 60-61 0.09302645856771521 62-63 0.09227798893861684 64-65 0.0 66-67 0.0 68-69 1.6632658424408226E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 1.6632658424408226E-5 78-79 1.6632658424408226E-5 80-81 8.316329212204113E-5 82-83 4.989797527322468E-5 84-85 0.0 86-87 6.486736785519208E-4 88-89 4.6571443588343036E-4 90-91 8.316329212204113E-5 92-93 6.819389954007373E-4 94-95 7.817349459471866E-4 96-97 3.326531684881645E-5 98-99 1.6632658424408226E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3006134.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.36174501167412 #Duplication Level Percentage of deduplicated Percentage of total 1 77.00315514911598 51.100637471000155 2 14.463455714525791 19.196403202300026 3 4.3131509866807605 8.586846779248777 4 1.7212444292150841 4.568991356573439 5 0.8402629949444835 2.7880659306625715 6 0.45581877088057726 1.8149357426826944 7 0.2946499489560705 1.368743934622788 8 0.18606021638930342 0.9877824509475089 9 0.13085562502189407 0.7815426858941559 >10 0.5607788763897732 6.2898643353758334 >50 0.023439655021399587 1.0444316172037968 >100 0.0065260705433613595 0.739808001188081 >500 4.010415438468898E-4 0.17305634287517663 >1k 1.0026038583904377E-4 0.14607658607890703 >5k 1.0026038583904377E-4 0.4128135633461985 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6898 0.22946415562313588 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5511 0.1833251611538275 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.326531684881645E-5 0.0 9 0.0 0.0 0.0 9.979595054644936E-5 0.0 10-11 0.0 0.0 0.0 1.1642860897085759E-4 0.0 12-13 0.0 0.0 0.0 1.330612673952658E-4 0.0 14-15 0.0 3.326531684881645E-5 0.0 1.330612673952658E-4 0.0 16-17 0.0 6.65306336976329E-5 0.0 1.9959190109289872E-4 0.0 18-19 0.0 8.316329212204113E-5 0.0 2.3285721794171518E-4 0.0 20-21 0.0 9.979595054644936E-5 0.0 3.160205100637563E-4 0.0 22-23 0.0 9.979595054644936E-5 0.0 3.9918380218579745E-4 0.0 24-25 0.0 9.979595054644936E-5 0.0 5.655103864298797E-4 0.0 26-27 0.0 9.979595054644936E-5 0.0 6.653063369763291E-4 0.0 28-29 0.0 9.979595054644936E-5 0.0 0.0014803065997723323 0.0 30-31 0.0 1.1642860897085759E-4 0.0 0.004640511700409895 0.0 32-33 0.0 1.330612673952658E-4 0.0 0.011709391530783392 0.0 34-35 0.0 1.6632658424408226E-4 0.0 0.021705619243852736 0.0 36-37 0.0 1.829592426684905E-4 0.0 0.04081654377349779 0.0 38-39 0.0 2.1622455951730694E-4 0.0 0.07449767708292446 0.0 40-41 0.0 2.494898763661234E-4 0.0 0.11571340465860804 0.0 42-43 0.0 2.993878516393481E-4 0.0 0.15940739833952844 0.0 44-45 0.0 2.993878516393481E-4 0.0 0.2084571080331083 0.0 46-47 0.0 2.993878516393481E-4 0.0 0.26853427026207083 0.0 48-49 0.0 2.993878516393481E-4 0.0 0.33792572120870196 0.0 50-51 0.0 2.993878516393481E-4 0.0 0.4176294203784662 0.0 52-53 0.0 2.993878516393481E-4 0.0 0.4985140382963634 0.0 54-55 0.0 2.993878516393481E-4 0.0 0.5850371274201349 0.0 56-57 0.0 2.993878516393481E-4 0.0 0.6892906304243257 0.0 58-59 0.0 2.993878516393481E-4 0.0 0.8113577106010577 0.0 60-61 0.0 3.160205100637563E-4 0.0 0.9426559162033362 0.0 62-63 0.0 3.3265316848816453E-4 0.0 1.076814939054613 0.0 64-65 0.0 3.3265316848816453E-4 0.0 1.2161966166511538 0.0 66-67 0.0 4.324491190346139E-4 0.0 1.3772340155162743 0.0 68-69 0.0 4.324491190346139E-4 0.0 1.565582239514273 0.0 70-71 0.0 4.324491190346139E-4 0.0 1.7666544472069443 0.0 72-73 0.0 4.324491190346139E-4 0.0 1.9807167611290781 0.0 74-75 0.0 4.324491190346139E-4 0.0 2.193880911496294 0.0 76-77 0.0 4.324491190346139E-4 0.0 2.4170911875518524 0.0 78-79 0.0 4.324491190346139E-4 0.0 2.6787062719093693 0.0 80-81 0.0 4.490817774590221E-4 0.0 2.9633575881846914 0.0 82-83 0.0 4.6571443588343036E-4 0.0 3.263976256547446 0.0 84-85 0.0 4.6571443588343036E-4 0.0 3.5613515565174407 0.0 86-87 0.0 4.6571443588343036E-4 0.0 3.874361555406379 0.0 88 0.0 4.6571443588343036E-4 0.0 4.133880924802421 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4820 0.0 52.05175 1 GTATCAA 8680 0.0 40.974842 1 TCAACGC 10325 0.0 33.89569 4 ATCAACG 10355 0.0 33.842915 3 CAACGCA 10555 0.0 33.10976 5 AACGCAG 10890 0.0 32.220806 6 TATCAAC 10955 0.0 32.075764 2 ACGCAGA 12645 0.0 27.636368 7 CGCAGAG 12815 0.0 27.26975 8 GTACATG 8900 0.0 25.1808 1 GCAGAGT 14120 0.0 24.520163 9 TACATGG 8915 0.0 24.21922 2 ACATGGG 8620 0.0 23.73797 3 GTGGTAT 2540 0.0 22.936602 1 TGGTATC 2515 0.0 22.444613 2 TAGACCG 170 3.432373E-6 22.134335 5 GAGTACT 9770 0.0 21.397154 12-13 AGAGTAC 13025 0.0 20.810793 10-11 CAGAGTA 14185 0.0 20.516464 10-11 GTACTTT 10920 0.0 19.100769 14-15 >>END_MODULE