FastQCFastQC Report
Wed 25 May 2016
SRR1032915_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032915_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3259256
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61150.028.0552941
GTACATG113200.027.2380391
GGTATCA42700.026.3080351
TCAACGC61700.026.0530974
TACATGG112100.025.953952
ATCAACG63300.025.691583
CAACGCA63900.025.3020675
AACGCAG65400.024.577266
ACATGGG114000.024.5318623
TATCAAC74200.022.8676592
CATGGGG75450.020.9937064
ACGCAGA77950.020.560017
CGCAGAG81600.019.3520748
GAGTACA81650.018.9390471
AGTACAT80450.017.760942
TATAGCG1600.001278871117.6258342
GTGTAAG13900.017.5835671
CGCCGTA4053.274181E-1117.40609494
TAGACCG1902.0242856E-417.3158115
TATACCG2203.197418E-517.090935
Produced by FastQC (version 0.11.5)