FastQCFastQC Report
Wed 25 May 2016
SRR1032915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032915_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3259256
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70340.215816124907034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53590.1644240280603917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62800.039.4983251
GTATCAA105600.037.6366421
TCAACGC120750.032.484044
ATCAACG122250.032.0854643
CAACGCA123000.031.9256065
AACGCAG126700.031.0304036
TATCAAC130200.030.234232
GTACATG115000.027.0844571
ACGCAGA146700.026.7354227
TACATGG115300.025.9017352
CGCAGAG152100.025.6934818
ACATGGG115750.024.5012053
GCAGAGT168800.022.9864629
GAGTACT94750.021.24366412-13
AGAGTAC140250.020.33029210-11
CATGGGG72650.019.5507074
GTACTTT103250.019.44929514-15
GAGTACA84650.019.4243071
CAGAGTA162100.019.19820810-11
AGTACTT97350.018.89094212-13
Produced by FastQC (version 0.11.5)