FastQCFastQC Report
Wed 25 May 2016
SRR1032914_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032914_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872512
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG62100.030.2741741
TACATGG60300.028.995432
ACATGGG60800.026.9789943
TAGACCG909.517662E-426.1121715
GTATCAA42300.024.0003341
TCAACGC41500.022.5382674
ATCAACG42800.021.8531073
CAACGCA43500.021.6100735
AACGCAG43700.021.2960596
CATGGGG40600.020.9540524
TATCAAC47250.020.6902372
GTCGTAC1605.4146098E-520.5627861
ACACCGT4250.019.9066936
CATGGGA20950.019.7430484
CCGCGCA1200.005064107519.584139
ATACCGC1550.001033372518.1942886
GTATAGA8300.018.1207331
CGTACAC1851.6502035E-417.7840333
ACGCAGA51600.017.762357
GTACACG2654.4792614E-717.7360971
Produced by FastQC (version 0.11.5)