FastQCFastQC Report
Wed 25 May 2016
SRR1032914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032914_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872512
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54800.2926550003417868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42680.22792911340488073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24000.1281700731423884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52550.031.5595381
GTATCAA77300.030.389191
GTACATG57950.028.2133371
TCAACGC87750.026.7016244
ATCAACG87900.026.5490063
TACATGG59400.026.5346782
CAACGCA89500.026.2811495
AACGCAG91050.025.8854186
TATCAAC91950.025.4308072
ACATGGG60750.024.9381963
TACACCG3850.023.2161525
ACGCAGA102600.022.8778767
CGCAGAG104300.022.5049888
ACCGTTC2756.002665E-1122.2368458
GAGTACT62850.021.93505312-13
CATGGGG41050.021.4330464
GCAGAGT112000.020.8436729
AGAGTAC85450.020.31132510-11
GTACTTT69800.019.54909114-15
TCGACCG1508.241946E-418.8173035
Produced by FastQC (version 0.11.5)