FastQCFastQC Report
Wed 25 May 2016
SRR1032913_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032913_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306882
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20200.036.0655331
GGTATCA12000.033.292751
TCAACGC21550.030.752744
ATCAACG21850.030.3305073
GTACCTG12500.029.7049661
CAACGCA22400.029.5846545
AACGCAG22750.029.5426886
TACCTGG12050.028.4739572
TATCAAC25250.027.3632682
GTACATG40550.027.1229551
TACATGG39550.025.788452
ACGCAGA26400.025.2801577
CGCAGAG27850.023.7951978
ACATGGG40550.022.9501933
ACCTGGG15700.021.8542163
TCTGTCG3700.021.5944718
GCAGAGT33050.020.1935319
CTGTCGC4300.019.6743059
GTCTCGC3801.2732926E-1118.5532381
CCTAGAC2854.821959E-818.1409423
Produced by FastQC (version 0.11.5)