FastQCFastQC Report
Wed 25 May 2016
SRR1032913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306882
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41290.31594283187005406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32400.24791832774496855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18950.14500161452985044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21850.050.531811
GTATCAA41750.043.8890881
ATAACGC1600.041.1849333
TATAACG1650.039.93692
ATCAACG51250.035.6342933
TCAACGC51750.035.1990474
CAACGCA51850.035.1244285
TATCAAC53450.034.2556462
AACGCAG54200.033.7751546
GTACCGG1351.331864E-831.3225541
ACGCAGA63950.028.62577
CGCAGAG65000.028.0898088
TAACGCA1853.2196112E-1027.9866714
TACCTGG13500.027.195132
GTACCTG13850.027.1386761
GTACATG41550.026.5732861
GTACACG1458.677998E-725.9221151
TACATGG41100.025.7674242
GCAGAGT71800.025.3173719
ACATGGG40600.024.9254343
Produced by FastQC (version 0.11.5)