##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032913_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1306882 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.783643817881035 34.0 31.0 34.0 31.0 34.0 2 32.84101089463318 34.0 31.0 34.0 31.0 34.0 3 32.91406339669534 34.0 31.0 34.0 31.0 34.0 4 36.34605343099071 37.0 37.0 37.0 35.0 37.0 5 36.331647386680665 37.0 37.0 37.0 35.0 37.0 6 36.39544197563361 37.0 37.0 37.0 35.0 37.0 7 36.36184751186411 37.0 37.0 37.0 35.0 37.0 8 36.29814780523414 37.0 37.0 37.0 35.0 37.0 9 38.12808960564152 39.0 39.0 39.0 37.0 39.0 10-11 38.14008571546628 39.0 39.0 39.0 37.0 39.0 12-13 38.13820299001746 39.0 39.0 39.0 37.0 39.0 14-15 39.66851291853435 41.0 40.0 41.0 37.0 41.0 16-17 39.67794835340911 41.0 40.0 41.0 37.0 41.0 18-19 39.71192923309067 41.0 40.0 41.0 37.0 41.0 20-21 39.72191598017265 41.0 40.0 41.0 37.0 41.0 22-23 39.67024605128849 41.0 40.0 41.0 37.0 41.0 24-25 39.64303739740849 41.0 40.0 41.0 37.0 41.0 26-27 39.560194416940476 41.0 40.0 41.0 36.5 41.0 28-29 39.4869877311035 41.0 40.0 41.0 36.0 41.0 30-31 39.35127310652378 41.0 39.0 41.0 36.0 41.0 32-33 39.189160536299376 41.0 39.0 41.0 35.0 41.0 34-35 39.06897026663463 41.0 39.0 41.0 35.0 41.0 36-37 38.95482453656872 41.0 39.0 41.0 35.0 41.0 38-39 38.84334813701619 41.0 39.0 41.0 35.0 41.0 40-41 38.726076263962625 41.0 39.0 41.0 35.0 41.0 42-43 38.572573116777185 41.0 39.0 41.0 35.0 41.0 44-45 38.3773592413087 41.0 38.0 41.0 35.0 41.0 46-47 38.11030031785578 41.0 38.0 41.0 34.0 41.0 48-49 37.86572850494536 40.5 38.0 41.0 33.5 41.0 50-51 37.696345194133826 40.0 38.0 41.0 33.0 41.0 52-53 37.54124588141852 40.0 37.5 41.0 33.0 41.0 54-55 37.388939475790465 40.0 37.0 41.0 33.0 41.0 56-57 37.17876212236453 40.0 37.0 41.0 33.0 41.0 58-59 37.008965231750075 40.0 36.0 41.0 32.0 41.0 60-61 36.81400998712967 39.5 36.0 41.0 32.0 41.0 62-63 36.575147947557625 39.0 35.0 41.0 32.0 41.0 64-65 36.27731501390332 39.0 35.0 41.0 31.0 41.0 66-67 36.00185670932801 38.5 35.0 41.0 31.0 41.0 68-69 35.70614408951994 37.5 35.0 40.0 31.0 41.0 70-71 35.377422368660675 37.0 35.0 40.0 31.0 41.0 72-73 35.040580557387734 36.5 35.0 39.0 30.0 41.0 74-75 34.709697968140965 36.0 35.0 39.0 30.0 41.0 76-77 34.363628468369754 35.5 35.0 38.5 30.0 40.5 78-79 34.02543687953465 35.0 34.0 37.0 30.0 39.5 80-81 33.702405037333136 35.0 34.0 37.0 29.0 39.0 82-83 33.39840475268616 35.0 34.0 36.5 29.0 39.0 84-85 33.12420402148013 35.0 34.0 36.0 29.0 37.5 86-87 32.9766708088412 35.0 34.0 36.0 29.0 37.0 88-89 32.786758483168335 35.0 34.0 35.5 29.0 37.0 90-91 32.61238734637098 35.0 34.0 35.0 29.0 36.0 92-93 32.4437546771629 35.0 34.0 35.0 29.0 36.0 94-95 32.341014720533295 35.0 34.0 35.0 29.0 36.0 96-97 32.273537702715316 35.0 34.0 35.0 29.0 36.0 98-99 32.159835011883246 35.0 34.0 35.0 29.0 36.0 100 32.071480057113035 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24858626886214097 1101 2 0.2553592172878183 1101 3 0.24679640001605918 1101 4 0.15933907231308098 1101 5 0.1789963598422446 1101 6 0.1578852451387931 1101 7 0.15824994719503138 1101 8 0.05643309134907071 1101 9 0.16732683023750639 1101 10-11 0.15014876796877985 1101 12-13 0.2341942829354906 1101 14-15 0.31853729112931006 1101 16-17 0.2965466913264052 1101 18-19 0.2841638226092513 1101 20-21 0.2951140771752563 1101 22-23 0.3741074911405491 1101 24-25 0.4379246063000011 1101 26-27 0.3768158720212753 1101 28-29 0.32111070734379865 1101 30-31 0.3321311191384737 1101 32-33 0.28649377973798096 1101 34-35 0.24218177957356346 1101 36-37 0.2620721552668641 1101 38-39 0.2790668333969464 1101 40-41 0.36647408826594585 1101 42-43 0.3598528484508705 1101 44-45 0.1949382594936111 1101 46-47 0.25946989058942904 1101 48-49 0.26757133546099254 1101 50-51 0.25926588227678593 1101 52-53 0.33362455121140755 1101 54-55 0.2681274368816915 1101 56-57 0.3310976171048381 1101 58-59 0.2857937093744596 1101 60-61 0.24815598725248833 1101 62-63 0.2570316162460742 1101 64-65 0.29159310458226884 1101 66-67 0.3215881793330553 1101 68-69 0.2703827818243667 1101 70-71 0.3502443542665148 1101 72-73 0.35250833584576213 1101 74-75 0.2840192214357842 1101 76-77 0.20971782513679216 1101 78-79 0.12454507801659531 1101 80-81 0.19442218873607686 1101 82-83 0.1882430733636724 1101 84-85 0.20809449727233442 1101 86-87 0.14949411456053951 1101 88-89 0.11994332834706967 1101 90-91 0.14066700543853017 1101 92-93 0.20100658069857502 1101 94-95 0.2079819615759959 1101 96-97 0.1369362073644922 1101 98-99 0.21455359151561737 1101 100 0.20510249936435088 1102 1 0.21259904804669105 1102 2 0.2506543503102989 1102 3 0.28687809748933546 1102 4 0.18016946723021476 1102 5 0.14693196216304472 1102 6 0.14211682854649865 1102 7 0.1755908540898048 1102 8 0.16696685577682047 1102 9 0.1751625734222273 1102 10-11 0.20859424126735604 1102 12-13 0.21677610194665675 1102 14-15 0.25960326550012525 1102 16-17 0.26410132486113724 1102 18-19 0.2321338996792761 1102 20-21 0.2603913006135272 1102 22-23 0.30407090373259393 1102 24-25 0.24347627731102506 1102 26-27 0.3373012013469605 1102 28-29 0.3091873896533457 1102 30-31 0.3442346430172236 1102 32-33 0.40337872446374234 1102 34-35 0.3733469768820825 1102 36-37 0.305885666319611 1102 38-39 0.3625764216865761 1102 40-41 0.3433778368547493 1102 42-43 0.2787300543636704 1102 44-45 0.42875166251267416 1102 46-47 0.3878155587687715 1102 48-49 0.31004687933557307 1102 50-51 0.41376845617933355 1102 52-53 0.37910838307018224 1102 54-55 0.41332530957839 1102 56-57 0.4264937616227016 1102 58-59 0.3691388463881822 1102 60-61 0.4315227610125447 1102 62-63 0.4604005266723519 1102 64-65 0.4870288914606178 1102 66-67 0.5253698684637271 1102 68-69 0.3575409077280227 1102 70-71 0.35357914405397395 1102 72-73 0.38154824048802993 1102 74-75 0.4158567783860434 1102 76-77 0.3880698853091644 1102 78-79 0.3953367608633016 1102 80-81 0.4532339862545456 1102 82-83 0.41096126689640755 1102 84-85 0.33427123918045965 1102 86-87 0.3910278747869853 1102 88-89 0.3204646508954454 1102 90-91 0.37068947644499417 1102 92-93 0.45100538032430393 1102 94-95 0.36869107067621343 1102 96-97 0.35261270529655064 1102 98-99 0.39628560555759407 1102 100 0.3446631143641099 1103 1 0.13800073655449552 1103 2 0.2042519176482287 1103 3 0.16527824609086395 1103 4 0.0707054073584743 1103 5 0.09418243256256176 1103 6 0.08436575674650726 1103 7 0.11892792157163967 1103 8 -0.008399574266377385 1103 9 0.039912249789651355 1103 10-11 0.06149621356280477 1103 12-13 0.11520076521399147 1103 14-15 0.11733048767202803 1103 16-17 0.18076292730388843 1103 18-19 0.23572051682739215 1103 20-21 0.20644728302901427 1103 22-23 0.18645067236826662 1103 24-25 0.1966461566066826 1103 26-27 0.2291156191351149 1103 28-29 0.200504175778363 1103 30-31 0.18020137278602988 1103 32-33 0.2087474133419036 1103 34-35 0.1644038823240379 1103 36-37 0.0869928050407367 1103 38-39 0.07309640090988268 1103 40-41 0.14490765290302932 1103 42-43 0.1580864813224636 1103 44-45 0.05776582437597 1103 46-47 0.1562864077581949 1103 48-49 0.09825530215278633 1103 50-51 0.07992841380038129 1103 52-53 0.09354950487414015 1103 54-55 0.0809675559840386 1103 56-57 0.12706684066701968 1103 58-59 0.04366537967274553 1103 60-61 0.05811154182643463 1103 62-63 0.07941991710796259 1103 64-65 0.11810381025149042 1103 66-67 0.1751564257584448 1103 68-69 0.14786885158049756 1103 70-71 0.1599597222010587 1103 72-73 0.04132612936022895 1103 74-75 0.05650566026272941 1103 76-77 0.09900006666953232 1103 78-79 0.14231714569081078 1103 80-81 0.13449947274682472 1103 82-83 0.09462375567390069 1103 84-85 0.03270129154644508 1103 86-87 0.04949030760957385 1103 88-89 0.07320879139726344 1103 90-91 0.013668283527877634 1103 92-93 0.00827343982453499 1103 94-95 0.08406381857761858 1103 96-97 0.08230268306364508 1103 98-99 0.12186639559946144 1103 100 0.14690555271339178 1104 1 0.14155975438352186 1104 2 0.16793680575921144 1104 3 0.10792170004617674 1104 4 0.09007164959867708 1104 5 0.04493757636424078 1104 6 0.06744025410547749 1104 7 0.08246704406260363 1104 8 -0.029437113920742775 1104 9 0.06351626579939307 1104 10-11 0.10841160162760843 1104 12-13 0.1461122565520654 1104 14-15 0.15912905738860417 1104 16-17 0.13772089895859807 1104 18-19 0.1260727800333612 1104 20-21 0.07934939863338997 1104 22-23 0.10088875703027611 1104 24-25 0.17570542312743243 1104 26-27 0.1999127011522006 1104 28-29 0.18270268852395333 1104 30-31 0.24658620737543657 1104 32-33 0.31762570795091705 1104 34-35 0.25022005296105476 1104 36-37 0.2624178342216794 1104 38-39 0.3393253441758972 1104 40-41 0.4535591206292935 1104 42-43 0.4199922499690558 1104 44-45 0.37370640763874263 1104 46-47 0.3971540803930935 1104 48-49 0.5224641342141041 1104 50-51 0.4150427396677685 1104 52-53 0.44525162448428546 1104 54-55 0.39432107767029123 1104 56-57 0.4028270195025243 1104 58-59 0.4313535258122414 1104 60-61 0.4689116059188052 1104 62-63 0.3802028231403156 1104 64-65 0.2787016384550256 1104 66-67 0.3339981120552551 1104 68-69 0.3189662552833852 1104 70-71 0.2788922282681412 1104 72-73 0.3198135718189761 1104 74-75 0.35676920672914036 1104 76-77 0.4023196839401919 1104 78-79 0.4371094741908834 1104 80-81 0.3929413606675354 1104 82-83 0.37901053306308796 1104 84-85 0.35015238495549994 1104 86-87 0.3740677820366045 1104 88-89 0.43385491141690125 1104 90-91 0.4411482185008282 1104 92-93 0.38549066286707756 1104 94-95 0.4055226320009595 1104 96-97 0.3743065486343653 1104 98-99 0.31126739904553347 1104 100 0.3179537187927295 1105 1 0.09395533672794443 1105 2 0.062478760274245815 1105 3 0.09496597220830694 1105 4 0.03990466084262323 1105 5 0.0654875596599922 1105 6 0.05230438036595331 1105 7 0.0950048971748032 1105 8 0.005011769472872629 1105 9 0.040419824824468265 1105 10-11 0.04416067698577564 1105 12-13 0.08428103067519288 1105 14-15 0.14462939523333773 1105 16-17 0.1504308971216588 1105 18-19 0.15099916855373152 1105 20-21 0.1425091760962971 1105 22-23 0.18107048419567917 1105 24-25 0.19295833186853173 1105 26-27 0.18754672453385268 1105 28-29 0.18991022187068296 1105 30-31 0.17666174709447802 1105 32-33 0.22290645209019289 1105 34-35 0.29379906941930756 1105 36-37 0.2829057808454394 1105 38-39 0.2884883056508514 1105 40-41 0.3631375827858534 1105 42-43 0.3379735927756542 1105 44-45 0.2537704531172622 1105 46-47 0.41826131950875123 1105 48-49 0.45905083892276366 1105 50-51 0.48455147367922535 1105 52-53 0.4526009559384363 1105 54-55 0.3872459429352304 1105 56-57 0.4467889982736253 1105 58-59 0.4155967482386842 1105 60-61 0.4195355175914699 1105 62-63 0.4122744397783933 1105 64-65 0.36941160653970684 1105 66-67 0.3870665908730899 1105 68-69 0.38134077201502947 1105 70-71 0.29735095803639666 1105 72-73 0.3357113418288904 1105 74-75 0.31368487230474784 1105 76-77 0.4111643408908279 1105 78-79 0.3601587565528135 1105 80-81 0.39349646803125893 1105 82-83 0.3862384767434648 1105 84-85 0.4033572907403311 1105 86-87 0.41609991005022096 1105 88-89 0.397174319173466 1105 90-91 0.3948997452474998 1105 92-93 0.41868028238623367 1105 94-95 0.4613115934479879 1105 96-97 0.46127498029358094 1105 98-99 0.39102534323078686 1105 100 0.430522617096635 1106 1 0.10591824430943575 1106 2 0.12787105171875623 1106 3 0.07661484644380323 1106 4 0.09305878292607872 1106 5 0.06624031636330585 1106 6 0.03232619703241113 1106 7 -0.007408826897474796 1106 8 -0.010477961577471717 1106 9 0.07224601054394952 1106 10-11 0.04560414158681425 1106 12-13 0.0057180503714775455 1106 14-15 0.05989447385913138 1106 16-17 0.047358127916666604 1106 18-19 0.12154437072559432 1106 20-21 0.13578952026033875 1106 22-23 0.18947541623307984 1106 24-25 0.21424209356843704 1106 26-27 0.2288860390656211 1106 28-29 0.16665698511899762 1106 30-31 0.20255448386046737 1106 32-33 0.211350819590713 1106 34-35 0.07286676441087536 1106 36-37 0.15552060433063275 1106 38-39 0.19943473633799869 1106 40-41 0.21154009415380415 1106 42-43 0.24309458539312345 1106 44-45 0.23123499082046806 1106 46-47 0.15840526252394227 1106 48-49 0.11940946569843902 1106 50-51 0.11940957342306291 1106 52-53 0.11580872087406391 1106 54-55 0.1812176078246992 1106 56-57 0.1605458568370821 1106 58-59 0.10385027097663624 1106 60-61 0.11355830252571764 1106 62-63 0.15770989472125763 1106 64-65 0.024580348649557493 1106 66-67 0.03791515297500325 1106 68-69 0.14178233480740232 1106 70-71 0.1468739227334268 1106 72-73 0.06130726570751932 1106 74-75 0.11389512327811957 1106 76-77 0.0838379958361486 1106 78-79 0.09705522339679362 1106 80-81 0.12446433747443564 1106 82-83 0.1859786377945838 1106 84-85 0.24621498806063613 1106 86-87 0.1840943190187545 1106 88-89 0.04948983760527881 1106 90-91 0.060252407166785815 1106 92-93 0.018910297053238878 1106 94-95 0.060815662623980415 1106 96-97 0.10411490561031655 1106 98-99 0.09410572171318421 1106 100 0.09284791013041627 1107 1 0.09339229032098473 1107 2 0.09425779138318546 1107 3 0.07163138376113665 1107 4 0.07415096832612988 1107 5 0.05836900855939575 1107 6 0.09390914785987547 1107 7 0.04665761135429847 1107 8 0.0503372559214057 1107 9 0.0945590181188507 1107 10-11 0.050115657339318886 1107 12-13 0.048040370702587154 1107 14-15 0.13078411918266397 1107 16-17 0.15369661641971533 1107 18-19 0.1680318237412095 1107 20-21 0.13440002932468786 1107 22-23 0.1559687923991575 1107 24-25 0.16469558322939548 1107 26-27 0.1648170474758075 1107 28-29 0.17454289049505434 1107 30-31 0.16732472797145448 1107 32-33 0.1118118307125826 1107 34-35 0.13339188609029407 1107 36-37 0.09569097036846586 1107 38-39 0.07708506164117779 1107 40-41 -0.029531758560153776 1107 42-43 0.15445317895910193 1107 44-45 0.14256352328447264 1107 46-47 0.09373814160061755 1107 48-49 0.0292989002231252 1107 50-51 -0.01766348470397361 1107 52-53 -0.01361162190661247 1107 54-55 0.02122190792307066 1107 56-57 -0.05171100699083553 1107 58-59 -0.09665703176810325 1107 60-61 -0.09538120117662174 1107 62-63 -0.03584005668614765 1107 64-65 -0.07079090967106794 1107 66-67 -0.08542097121618752 1107 68-69 -0.030071170886145637 1107 70-71 -0.05810580396043008 1107 72-73 -0.10919546972905181 1107 74-75 -0.10972025989083534 1107 76-77 -0.07107675929081836 1107 78-79 -0.0732574961853345 1107 80-81 -0.053160776089725914 1107 82-83 -0.06417871908488593 1107 84-85 -0.12532736667125022 1107 86-87 -0.1526678355631077 1107 88-89 -0.08386999403275297 1107 90-91 -0.034231739375016446 1107 92-93 -0.0162921193150396 1107 94-95 -0.016654766158666234 1107 96-97 -0.03496531868756847 1107 98-99 -0.024824162436523523 1107 100 -0.019487335237450054 1108 1 0.10562406467544605 1108 2 0.10669743597297554 1108 3 0.07311688960185592 1108 4 -0.0017856092197661155 1108 5 0.010990161223794814 1108 6 0.05777493074276663 1108 7 0.009732625208322077 1108 8 0.08091238113448185 1108 9 0.126287248381594 1108 10-11 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-0.13587072136376577 2214 62-63 -0.24452254892386094 2214 64-65 -0.1661061092029925 2214 66-67 -0.2503846777879417 2214 68-69 -0.21951490169475107 2214 70-71 -0.19234059716916363 2214 72-73 -0.23650253359537743 2214 74-75 -0.17437121817752654 2214 76-77 -0.09576913811578436 2214 78-79 -0.18463991912840072 2214 80-81 -0.11047774260279652 2214 82-83 -0.09823748694214629 2214 84-85 -0.17762833768620823 2214 86-87 -0.14944757643625195 2214 88-89 -0.1757211118729458 2214 90-91 -0.21082861440960698 2214 92-93 -0.1693062414919595 2214 94-95 -0.20869666674634146 2214 96-97 -0.16143739606667395 2214 98-99 -0.24593542039819383 2214 100 -0.2212174663026616 2215 1 -0.17096188658705813 2215 2 -0.16215854803473206 2215 3 -0.15185798186041666 2215 4 -0.09417385960288982 2215 5 -0.09791523425752047 2215 6 -0.09879408348958663 2215 7 -0.12381185865588407 2215 8 -0.09395266622546927 2215 9 -0.12973120823257034 2215 10-11 -0.13343724900772003 2215 12-13 -0.1481049140106947 2215 14-15 -0.25251773390893106 2215 16-17 -0.2046960596867251 2215 18-19 -0.1760696817121854 2215 20-21 -0.2227023124799672 2215 22-23 -0.19191340523366307 2215 24-25 -0.2007112259247137 2215 26-27 -0.22925428046367102 2215 28-29 -0.1772318578654577 2215 30-31 -0.25371433337159743 2215 32-33 -0.26127381955738826 2215 34-35 -0.25914785643857385 2215 36-37 -0.1595703423966981 2215 38-39 -0.19949940571671476 2215 40-41 -0.12190093528757728 2215 42-43 -0.1289413508938182 2215 44-45 -0.13734638569287227 2215 46-47 0.0018214691420936902 2215 48-49 -0.0565791006544174 2215 50-51 0.04361873325140664 2215 52-53 0.1561985269909485 2215 54-55 0.09100474178703877 2215 56-57 0.1518146181231117 2215 58-59 0.15271095159021542 2215 60-61 0.16649508179021666 2215 62-63 0.1988982228300884 2215 64-65 0.14366696085060937 2215 66-67 0.08299216053374181 2215 68-69 0.07886542929421836 2215 70-71 0.09391788512392196 2215 72-73 0.056754872680627955 2215 74-75 0.08011975175344332 2215 76-77 0.0895166254854729 2215 78-79 0.12753451303713348 2215 80-81 0.20611466359676456 2215 82-83 0.034837855204301604 2215 84-85 -0.06945058894950051 2215 86-87 -0.17346676543910178 2215 88-89 -0.1857101780596082 2215 90-91 -0.1300783543705677 2215 92-93 -0.09245500986668276 2215 94-95 -0.07406967813898291 2215 96-97 -0.047847853269182394 2215 98-99 -0.06299137649534003 2215 100 -0.13683571937259487 2216 1 -0.48399195829874486 2216 2 -0.5031643257461553 2216 3 -0.47040469238567084 2216 4 -0.29677583645030836 2216 5 -0.2878163597794696 2216 6 -0.31854357418729506 2216 7 -0.3045806602984982 2216 8 -0.3242781059752886 2216 9 -0.4755406416829402 2216 10-11 -0.4584175632099061 2216 12-13 -0.4933004957230125 2216 14-15 -0.628646205713089 2216 16-17 -0.6832186736717105 2216 18-19 -0.6208888452845116 2216 20-21 -0.6311969650065734 2216 22-23 -0.6494674338188062 2216 24-25 -0.6734970653142867 2216 26-27 -0.7274787459170042 2216 28-29 -0.7506414219925972 2216 30-31 -0.8375215506145111 2216 32-33 -0.8975595409525781 2216 34-35 -0.8532139629931237 2216 36-37 -0.8393521450940398 2216 38-39 -0.8898662879840131 2216 40-41 -0.7890154990053446 2216 42-43 -0.9050908529896233 2216 44-45 -0.8533025977360111 2216 46-47 -0.7887513414199248 2216 48-49 -0.74613416951658 2216 50-51 -0.6861243600636939 2216 52-53 -0.6637618084341739 2216 54-55 -0.6985260860519347 2216 56-57 -0.8906025570431595 2216 58-59 -0.885520131983526 2216 60-61 -0.9873199295035278 2216 62-63 -0.8689078120609324 2216 64-65 -0.8252487429979354 2216 66-67 -0.8903146200496792 2216 68-69 -0.8005064833867266 2216 70-71 -0.860340327526032 2216 72-73 -0.8009422943303903 2216 74-75 -0.771373479221765 2216 76-77 -0.79039489076645 2216 78-79 -0.924996466508901 2216 80-81 -0.9893730344853111 2216 82-83 -0.8450527912925523 2216 84-85 -0.8073109132976057 2216 86-87 -0.791501886026154 2216 88-89 -0.9394518843386734 2216 90-91 -0.8475191284137367 2216 92-93 -0.9367127569605671 2216 94-95 -0.9497180452411875 2216 96-97 -0.8556849680415937 2216 98-99 -0.8677952529720727 2216 100 -0.9567606364153178 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 7.0 5 16.0 6 58.0 7 104.0 8 237.0 9 414.0 10 741.0 11 1263.0 12 2068.0 13 3438.0 14 3444.0 15 4007.0 16 5097.0 17 7589.0 18 9250.0 19 5018.0 20 3238.0 21 2928.0 22 2934.0 23 3226.0 24 3363.0 25 4020.0 26 5078.0 27 6361.0 28 8005.0 29 10225.0 30 12838.0 31 15806.0 32 20604.0 33 27126.0 34 37508.0 35 59443.0 36 110497.0 37 252896.0 38 449203.0 39 228026.0 40 799.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.134078212290504 19.717127599418156 13.365583194771666 22.783210993519674 2 16.160268976905265 20.470541244094704 40.04872681306136 23.32046296593867 3 18.264903074349707 26.459700140334448 30.02231271779003 25.253084067525815 4 12.458331930368026 17.27966047219221 38.775027625662325 31.48697997177744 5 12.206008045108927 37.4402107001326 35.14199073978426 15.211790514974203 6 29.556428224901786 38.23204876473586 18.2274728621429 13.984050148219454 7 26.545522438310716 32.75064428651008 22.86268280290648 17.841150472272723 8 25.22258876538811 35.199714939252175 20.818706728429827 18.758989566929888 9 25.25165108362047 16.561338588642013 21.815499487706877 36.37151084003064 10-11 23.744951724792294 26.576462144248676 28.77164885582631 20.90693727513272 12-13 25.015437518794748 24.077173055542165 29.197073031132902 21.71031639453018 14-15 22.1705597187805 25.657530017113285 27.10073882098541 25.07117144312081 16-17 20.91719068745304 29.295873690203095 27.28322067332781 22.503714949016054 18-19 20.712085544229595 27.381560426955964 30.558364409266503 21.34798961954794 20-21 22.026510427108185 26.824839580007986 30.14598869676069 21.00266129612314 22-23 21.559761844822663 26.791080122949406 30.26994082849292 21.379217203735003 24-25 21.23107224321799 26.7872186900527 30.394482418773915 21.587226647955394 26-27 21.10134005913646 26.646339783656053 30.499315890322066 21.753004266885416 28-29 21.284270980602972 26.769694036261093 30.393163308839142 21.55287167429679 30-31 21.567375976080083 27.26442735040773 29.933415908168215 21.234780765343977 32-33 21.086492675184097 26.932875879919244 30.480333737929094 21.500297706967565 34-35 21.200852640199614 26.51519674251644 30.475075199191753 21.8088754180922 36-37 21.792629324400668 27.105918249556403 29.72260453808504 21.378847887957892 38-39 21.61057138417186 27.2462928274028 29.78701986268285 21.356115925742486 40-41 21.334951772205876 27.03256093835769 29.955014442691265 21.677472846745168 42-43 21.315405613387597 27.09430047052608 29.78721041972151 21.803083496364813 44-45 21.5844832525696 27.228496752853797 29.575304836478345 21.611715158098256 46-47 21.602661066088835 27.613501907355275 29.041442411813307 21.74239461474258 48-49 21.55853647045665 27.527341686721513 29.006912554481325 21.90720928834051 50-51 21.65826547262462 27.31700590637137 28.880087637987696 22.14464098301631 52-53 21.72843771329032 27.412272806312664 28.675066395289246 22.184223085107767 54-55 21.84069627676404 27.99689430287671 28.438119329555526 21.72429009080372 56-57 21.469685782388375 27.650368568434857 28.592814600579658 22.28713104859711 58-59 21.37404141676936 27.01717006087908 29.092603754452583 22.516184767898977 60-61 21.828217104610637 27.587808669218877 28.54237934601167 22.041594880158815 62-63 21.556009375774245 27.509036302596567 28.649530847499683 22.285423474129505 64-65 21.564035620660473 27.226406056552925 28.91986422645656 22.28969409633004 66-67 21.454478257775573 27.37456585565174 28.627816916765948 22.54313896980674 68-69 22.000472881616613 27.516583376757332 28.521839402363337 21.961104339262718 70-71 22.06010183015758 27.23769246190551 28.584868411991287 22.117337295945617 72-73 21.695531807768415 27.402550498055678 28.79131398244065 22.110603711735262 74-75 21.676517084174392 27.454506221678777 28.591678514204037 22.277298179942797 76-77 21.806231783919117 27.499098999832427 28.320788197242138 22.373881019006323 78-79 21.880705373553237 27.691597252085497 28.397437565135952 22.030259809225317 80-81 21.731353859079533 27.380997016556236 28.53726320493328 22.35038591943095 82-83 21.524891344270323 27.440507162095983 28.62144955925563 22.41315193437806 84-85 21.596249699666842 27.434305469047704 28.434089688280963 22.535355143004494 86-87 21.758978691103643 27.49286081574936 28.309084592829294 22.439075900317707 88-89 21.337088341136923 27.446117729518537 28.450649908139784 22.766144021204752 90-91 21.29364544042091 27.256508915696575 28.50650843745851 22.943337206424 92-93 21.562538976625884 27.48086462877418 28.237824180314387 22.71877221428555 94-95 21.31032010415714 27.16927410231228 28.515083541113263 23.00532225241732 96-97 21.260231436881302 27.2449476959994 28.48086930771709 23.01395155940221 98-99 21.240632283557353 27.253990796414673 28.443271848567814 23.062105071460163 100 21.39665249043142 27.212556298120262 28.2348368100563 23.155954401392016 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 57.0 1 61.5 2 337.0 3 583.5 4 343.0 5 152.0 6 357.5 7 846.5 8 1414.5 9 1574.5 10 1155.5 11 786.5 12 788.5 13 918.0 14 1021.5 15 1103.0 16 1222.0 17 1407.0 18 1594.5 19 1777.0 20 2082.5 21 2492.5 22 3080.0 23 4042.5 24 5243.5 25 6824.5 26 9150.0 27 11841.5 28 14962.0 29 18708.5 30 22476.0 31 25743.0 32 28715.5 33 31771.0 34 34651.5 35 36965.5 36 39046.0 37 41269.0 38 42850.0 39 43418.0 40 43793.5 41 44259.0 42 44312.5 43 43972.5 44 43315.5 45 42673.5 46 42501.5 47 41906.0 48 41304.5 49 40519.0 50 39370.5 51 38168.5 52 35962.0 53 34073.5 54 33057.0 55 31828.0 56 30153.5 57 28180.0 58 26230.5 59 24164.0 60 21448.0 61 18311.0 62 15707.0 63 13444.0 64 10781.5 65 8410.5 66 6671.5 67 5151.0 68 3924.5 69 2929.0 70 2063.0 71 1498.5 72 1119.0 73 822.5 74 628.0 75 474.0 76 339.5 77 224.0 78 159.0 79 103.0 80 55.0 81 28.0 82 14.5 83 10.5 84 7.5 85 3.0 86 1.0 87 1.0 88 1.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.295540071712672E-4 2 3.0607200956168955E-4 3 3.0607200956168955E-4 4 0.009641268301193223 5 7.651800239042239E-5 6 0.002754648086055206 7 7.651800239042239E-4 8 0.14515465053463128 9 9.94734031075491E-4 10-11 0.0 12-13 7.651800239042239E-5 14-15 1.9129500597605598E-4 16-17 0.0 18-19 1.9129500597605598E-4 20-21 0.0 22-23 2.295540071712672E-4 24-25 3.8259001195211197E-4 26-27 0.005662332176891257 28-29 0.008378721261751251 30-31 0.01442364345059462 32-33 0.017446104545016307 34-35 0.026934336841428683 36-37 0.03948328923345796 38-39 0.043079635345807804 40-41 0.05241483163743934 42-43 0.06863664814420889 44-45 0.10926770741352318 46-47 0.11787598268244569 48-49 0.11504481659400008 50-51 0.11569521961431867 52-53 0.11041547744937953 54-55 0.11776120567886007 56-57 0.0987082230836449 58-59 0.090214724818308 60-61 0.09308414990794885 62-63 0.09002342981233195 64-65 0.0 66-67 7.651800239042239E-5 68-69 7.651800239042239E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 7.651800239042239E-5 78-79 0.0 80-81 2.295540071712672E-4 82-83 1.5303600478084478E-4 84-85 0.0 86-87 7.651800239042239E-4 88-89 3.8259001195211197E-4 90-91 1.147770035856336E-4 92-93 6.886620215138015E-4 94-95 8.034390250994351E-4 96-97 2.295540071712672E-4 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1306882.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.86540110631785 #Duplication Level Percentage of deduplicated Percentage of total 1 85.339509118135 64.7431608946358 2 9.969751866802055 15.12718448610798 3 2.3154462120028017 5.269867668410906 4 0.866852891664887 2.630565693053126 5 0.43238235156650523 1.640143026644293 6 0.2647006930506956 1.2048974550846807 7 0.18098716403632617 0.9611464656297583 8 0.11708705796262568 0.7106285293354627 9 0.0913403909745605 0.6236617858643634 >10 0.406543933731388 5.119895247162353 >50 0.011362817038234968 0.5695867780998701 >100 0.0033293031922301563 0.47086409938714757 >500 4.035427672661626E-4 0.21935600140926947 >1k 3.02657075449622E-4 0.7090418691749729 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4129 0.31594283187005406 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3240 0.24791832774496855 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1895 0.14500161452985044 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.651800239042239E-5 0.0 3 0.0 0.0 0.0 7.651800239042239E-5 0.0 4 0.0 0.0 0.0 7.651800239042239E-5 0.0 5 0.0 0.0 0.0 7.651800239042239E-5 0.0 6 0.0 0.0 0.0 7.651800239042239E-5 0.0 7 0.0 0.0 0.0 7.651800239042239E-5 0.0 8 0.0 0.0 0.0 7.651800239042239E-5 0.0 9 0.0 0.0 0.0 7.651800239042239E-5 0.0 10-11 0.0 0.0 0.0 7.651800239042239E-5 0.0 12-13 0.0 0.0 0.0 7.651800239042239E-5 0.0 14-15 0.0 0.0 0.0 7.651800239042239E-5 0.0 16-17 0.0 0.0 0.0 7.651800239042239E-5 0.0 18-19 0.0 0.0 0.0 1.1477700358563358E-4 0.0 20-21 0.0 0.0 0.0 1.5303600478084478E-4 0.0 22-23 0.0 0.0 0.0 1.9129500597605598E-4 0.0 24-25 0.0 0.0 0.0 3.0607200956168955E-4 0.0 26-27 0.0 0.0 0.0 4.973670155377456E-4 0.0 28-29 0.0 0.0 0.0 0.0013773240430276033 0.0 30-31 0.0 0.0 0.0 0.004782375149401399 0.0 32-33 0.0 0.0 0.0 0.012625470394419695 0.0 34-35 0.0 0.0 0.0 0.022878882714736298 0.0 36-37 0.0 3.8259001195211194E-5 0.0 0.041051908282461615 0.0 38-39 0.0 1.1477700358563358E-4 0.0 0.07452853432827142 0.0 40-41 0.0 1.5303600478084478E-4 0.0 0.12170188280196681 0.0 42-43 0.0 1.5303600478084478E-4 0.0 0.16994648330912815 0.0 44-45 0.0 1.5303600478084478E-4 0.0 0.21941537185453622 0.0 46-47 0.0 1.5303600478084478E-4 0.0 0.2781046796879902 0.0 48-49 0.0 1.5303600478084478E-4 0.0 0.34911338590630214 0.0 50-51 0.0 1.5303600478084478E-4 0.0 0.43133197947481106 0.0 52-53 0.0 1.5303600478084478E-4 0.0 0.5145070480732001 0.0 54-55 0.0 1.5303600478084478E-4 0.0 0.6069407949608305 0.0 56-57 7.651800239042239E-5 1.5303600478084478E-4 0.0 0.709398400161606 0.0 58-59 7.651800239042239E-5 1.5303600478084478E-4 0.0 0.8252466557807056 0.0 60-61 7.651800239042239E-5 1.5303600478084478E-4 0.0 0.9557863678587661 0.0 62-63 7.651800239042239E-5 1.5303600478084478E-4 0.0 1.0911084550862282 0.0 64-65 1.5303600478084478E-4 1.5303600478084478E-4 0.0 1.2352683715897839 0.0 66-67 1.5303600478084478E-4 1.5303600478084478E-4 0.0 1.396147471615647 0.0 68-69 1.5303600478084478E-4 1.5303600478084478E-4 0.0 1.5732483881482797 0.0 70-71 1.5303600478084478E-4 1.5303600478084478E-4 0.0 1.7718891223538162 0.0 72-73 1.5303600478084478E-4 1.5303600478084478E-4 0.0 1.9957425383469969 0.0 74-75 1.5303600478084478E-4 1.5303600478084478E-4 0.0 2.216267421236194 0.0 76-77 1.5303600478084478E-4 1.5303600478084478E-4 0.0 2.4429520033178207 0.0 78-79 1.5303600478084478E-4 1.5303600478084478E-4 0.0 2.692324173108207 0.0 80-81 1.5303600478084478E-4 1.5303600478084478E-4 0.0 2.969089787754365 0.0 82-83 1.5303600478084478E-4 1.5303600478084478E-4 0.0 3.277304301382986 0.0 84-85 1.5303600478084478E-4 1.5303600478084478E-4 0.0 3.5746149996709726 0.0 86-87 1.5303600478084478E-4 2.295540071712672E-4 0.0 3.8883388094717044 0.0 88 1.5303600478084478E-4 2.295540071712672E-4 0.0 4.137328389250139 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2185 0.0 50.53181 1 GTATCAA 4175 0.0 43.889088 1 ATAACGC 160 0.0 41.184933 3 TATAACG 165 0.0 39.9369 2 ATCAACG 5125 0.0 35.634293 3 TCAACGC 5175 0.0 35.199047 4 CAACGCA 5185 0.0 35.124428 5 TATCAAC 5345 0.0 34.255646 2 AACGCAG 5420 0.0 33.775154 6 GTACCGG 135 1.331864E-8 31.322554 1 ACGCAGA 6395 0.0 28.6257 7 CGCAGAG 6500 0.0 28.089808 8 TAACGCA 185 3.2196112E-10 27.986671 4 TACCTGG 1350 0.0 27.19513 2 GTACCTG 1385 0.0 27.138676 1 GTACATG 4155 0.0 26.573286 1 GTACACG 145 8.677998E-7 25.922115 1 TACATGG 4110 0.0 25.767424 2 GCAGAGT 7180 0.0 25.317371 9 ACATGGG 4060 0.0 24.925434 3 >>END_MODULE