FastQCFastQC Report
Wed 25 May 2016
SRR1032912_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032912_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477453
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43250.029.7769261
GTACATG89650.028.3112371
TCAACGC45450.027.0946044
TACATGG89700.026.8282852
ATCAACG46550.026.4543443
CAACGCA46850.026.3847395
AACGCAG48350.025.5656666
ACATGGG93700.024.7299233
TATCAAC53250.023.3906152
GGTATCA30250.022.9959721
CGTTATA1050.00233570322.3818552
CATGGGG64150.022.0538924
GGACCGT2659.058567E-1021.283026
ACGCAGA58550.020.9513237
ACCGCGT2902.7866918E-919.4478848
CGCAGAG64100.019.283548
TATACCG1250.006412464718.8003795
GAGTACA67000.018.3096941
AGTACAT66300.017.1560462
GTATAGG10900.016.8171921
Produced by FastQC (version 0.11.5)