##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032912_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2477453 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45802644893768 33.0 31.0 34.0 30.0 34.0 2 31.516057015006943 34.0 31.0 34.0 29.0 34.0 3 31.68620474333923 34.0 31.0 34.0 30.0 34.0 4 34.99006358546459 37.0 35.0 37.0 33.0 37.0 5 35.08067922983806 37.0 35.0 37.0 33.0 37.0 6 35.13476461511076 37.0 36.0 37.0 33.0 37.0 7 35.04709231618118 37.0 35.0 37.0 33.0 37.0 8 35.05933795716811 37.0 36.0 37.0 33.0 37.0 9 36.68789236364928 39.0 38.0 39.0 33.0 39.0 10-11 36.67296655072771 39.0 38.0 39.0 33.0 39.0 12-13 36.61114277445425 39.0 38.0 39.0 33.0 39.0 14-15 38.063119663622274 41.0 39.0 41.0 33.0 41.0 16-17 38.07454450195422 41.0 39.0 41.0 33.0 41.0 18-19 38.08015247917922 41.0 39.0 41.0 33.5 41.0 20-21 38.11170403636315 41.0 39.0 41.0 33.5 41.0 22-23 38.098069670746526 41.0 39.0 41.0 33.0 41.0 24-25 38.12504455180381 41.0 39.0 41.0 33.0 41.0 26-27 38.09762687728082 41.0 39.0 41.0 33.0 41.0 28-29 38.0739091720408 41.0 39.0 41.0 33.0 41.0 30-31 38.023784507718204 41.0 39.0 41.0 33.0 41.0 32-33 37.97102588828123 41.0 38.5 41.0 33.0 41.0 34-35 37.89594757196201 41.0 38.0 41.0 33.0 41.0 36-37 37.801932872187685 41.0 38.0 41.0 32.5 41.0 38-39 37.701419966392905 40.0 38.0 41.0 32.0 41.0 40-41 37.60829549541404 40.0 38.0 41.0 32.0 41.0 42-43 37.48273408214 40.0 38.0 41.0 32.0 41.0 44-45 37.34552966292398 40.0 38.0 41.0 31.0 41.0 46-47 37.20813956914621 40.0 37.5 41.0 31.0 41.0 48-49 37.05829051045569 40.0 37.0 41.0 31.0 41.0 50-51 36.90520163248304 40.0 37.0 41.0 30.5 41.0 52-53 36.735920721805826 40.0 36.0 41.0 30.0 41.0 54-55 36.554699725887836 40.0 36.0 41.0 30.0 41.0 56-57 36.357728481630126 39.5 35.0 41.0 30.0 41.0 58-59 36.13649138853492 39.0 35.0 41.0 30.0 41.0 60-61 35.880537794258856 39.0 35.0 41.0 29.5 41.0 62-63 35.61675458626259 38.5 35.0 40.5 29.0 41.0 64-65 35.33148963875399 38.0 35.0 40.0 29.0 41.0 66-67 35.029719635448174 37.0 34.5 40.0 29.0 41.0 68-69 34.71586342909431 37.0 34.0 40.0 28.0 41.0 70-71 34.38279394200415 36.0 34.0 39.0 28.0 41.0 72-73 34.09560100635613 36.0 34.0 39.0 28.0 41.0 74-75 33.82779289859384 35.0 34.0 39.0 28.0 40.5 76-77 33.5158880915198 35.0 34.0 37.5 27.5 39.5 78-79 33.16641566964136 35.0 34.0 37.0 27.0 39.0 80-81 32.86060139183266 35.0 33.5 36.5 27.0 39.0 82-83 32.561847792874374 35.0 33.0 36.0 26.0 38.0 84-85 32.40945781817052 35.0 33.0 36.0 26.5 37.0 86-87 32.19748568388583 35.0 33.0 35.5 26.0 37.0 88-89 31.980829101500614 35.0 33.0 35.0 26.0 36.5 90-91 31.792422701863565 35.0 33.0 35.0 25.5 36.0 92-93 31.61199586833736 35.0 33.0 35.0 25.0 36.0 94-95 31.43278944141423 35.0 33.0 35.0 25.0 36.0 96-97 31.26256259957303 35.0 33.0 35.0 24.0 35.5 98-99 31.085773978356 35.0 33.0 35.0 24.0 35.0 100 30.956189279877357 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26976305199405104 1101 2 0.36423055204712895 1101 3 0.33075734689305847 1101 4 0.3066169690977816 1101 5 0.2597499498425506 1101 6 0.23976632117796015 1101 7 0.25661891793273384 1101 8 0.260615232068119 1101 9 0.24505387440085968 1101 10-11 0.3052043060936711 1101 12-13 0.09161812485402976 1101 14-15 0.08701425686262354 1101 16-17 0.13274054364231347 1101 18-19 0.21397683730387485 1101 20-21 0.1104742731472399 1101 22-23 0.19450154662101937 1101 24-25 0.18647555401130944 1101 26-27 0.21974142128773622 1101 28-29 0.15318637241741584 1101 30-31 0.20178802382399397 1101 32-33 0.17093898129189 1101 34-35 0.17336731840670438 1101 36-37 0.16595718634339107 1101 38-39 0.21270941858796988 1101 40-41 0.21380203192305913 1101 42-43 0.23413542713841196 1101 44-45 0.17091600029324638 1101 46-47 0.13875485567846368 1101 48-49 0.12722123024614262 1101 50-51 0.20334290907030805 1101 52-53 0.11804505352802153 1101 54-55 0.14368027564919572 1101 56-57 0.182268911972443 1101 58-59 0.1843205960542562 1101 60-61 0.1649135608622032 1101 62-63 0.20096242326120262 1101 64-65 0.18603221295490613 1101 66-67 0.1066531729278779 1101 68-69 0.1439603787553949 1101 70-71 0.15209652286149122 1101 72-73 0.14856860565417662 1101 74-75 0.1562952461777556 1101 76-77 0.21221001134143336 1101 78-79 0.29243583279624374 1101 80-81 0.24517690485528476 1101 82-83 0.15169357028877073 1101 84-85 0.14199263072133306 1101 86-87 0.17802553494335882 1101 88-89 0.1861746332218921 1101 90-91 0.11342818912447683 1101 92-93 0.17932350817527976 1101 94-95 0.2617821064948309 1101 96-97 0.1990519219256619 1101 98-99 0.22972209793303833 1101 100 0.2719919399770028 1102 1 0.3082356806821167 1102 2 0.3203819963231105 1102 3 0.31342564334084244 1102 4 0.36550456513145946 1102 5 0.32291219533616555 1102 6 0.31400637696662415 1102 7 0.3936122068388599 1102 8 0.36017291887590375 1102 9 0.3790395119547796 1102 10-11 0.38160147121478616 1102 12-13 0.39368747674179616 1102 14-15 0.4865035790538883 1102 16-17 0.4649778883527702 1102 18-19 0.47133925596173754 1102 20-21 0.4143385390209531 1102 22-23 0.39561026790886444 1102 24-25 0.42331311263053806 1102 26-27 0.41602093658457306 1102 28-29 0.4334910944731334 1102 30-31 0.47663768043381083 1102 32-33 0.4641573680436508 1102 34-35 0.477460380475506 1102 36-37 0.4986799394197945 1102 38-39 0.5382642689497743 1102 40-41 0.5688051857062248 1102 42-43 0.5094199532286225 1102 44-45 0.5186938564258909 1102 46-47 0.4996612647143479 1102 48-49 0.5381231338774981 1102 50-51 0.486638827957357 1102 52-53 0.4727314577842492 1102 54-55 0.5003002819959335 1102 56-57 0.5496155029483134 1102 58-59 0.5047661217957895 1102 60-61 0.6253218288671718 1102 62-63 0.5348779332142328 1102 64-65 0.5777567642753851 1102 66-67 0.6011599094870803 1102 68-69 0.5814774699095295 1102 70-71 0.5661917788664041 1102 72-73 0.5709257941250385 1102 74-75 0.564240037342465 1102 76-77 0.49523305942388873 1102 78-79 0.5135197547704706 1102 80-81 0.46273740460259205 1102 82-83 0.46161388339479714 1102 84-85 0.4857634192454796 1102 86-87 0.5211220798857497 1102 88-89 0.4553410158521878 1102 90-91 0.413061880147751 1102 92-93 0.44355843172052545 1102 94-95 0.4285212514082275 1102 96-97 0.40300766709998115 1102 98-99 0.4465491961782817 1102 100 0.4791947106049008 1103 1 0.2584842730592243 1103 2 0.29283939409988236 1103 3 0.29204547919821167 1103 4 0.3055319781393777 1103 5 0.24534298816365663 1103 6 0.23453259084602252 1103 7 0.28818342501011784 1103 8 0.23768769643870513 1103 9 0.31831085651860747 1103 10-11 0.3283985859880829 1103 12-13 0.2925170876691894 1103 14-15 0.3145730964904274 1103 16-17 0.3635910767844237 1103 18-19 0.34933673420894706 1103 20-21 0.37375051627944345 1103 22-23 0.3424884934730912 1103 24-25 0.3385471523290846 1103 26-27 0.3733750324508165 1103 28-29 0.39382364871597275 1103 30-31 0.39843605303421725 1103 32-33 0.3741076953074014 1103 34-35 0.312684037382958 1103 36-37 0.3135370883754973 1103 38-39 0.33771097748302736 1103 40-41 0.39315671865443846 1103 42-43 0.3941352192857437 1103 44-45 0.32311889221678314 1103 46-47 0.3975338597870248 1103 48-49 0.3972239323309097 1103 50-51 0.37104329636913036 1103 52-53 0.30596119190246895 1103 54-55 0.26627247563118317 1103 56-57 0.281347431784269 1103 58-59 0.2576925899849485 1103 60-61 0.30937913699517594 1103 62-63 0.29474936041348343 1103 64-65 0.28154259519597247 1103 66-67 0.27007595219526337 1103 68-69 0.3420325452038213 1103 70-71 0.30837609856489223 1103 72-73 0.2949246018573959 1103 74-75 0.2798518825672147 1103 76-77 0.2721734292708007 1103 78-79 0.28884240648348225 1103 80-81 0.27235398840512914 1103 82-83 0.30705582285748534 1103 84-85 0.30710386654404687 1103 86-87 0.3117181069816368 1103 88-89 0.27497691682993874 1103 90-91 0.28734960695684464 1103 92-93 0.3311003021170684 1103 94-95 0.33799025016259776 1103 96-97 0.3132822088316374 1103 98-99 0.3572889052466799 1103 100 0.3817263735438239 1104 1 0.07158248521666266 1104 2 0.08041093927518617 1104 3 0.129434666820039 1104 4 0.10790373930456809 1104 5 0.19045587796296815 1104 6 0.14796873312397452 1104 7 0.15224689171853356 1104 8 0.14041293982165826 1104 9 0.19147635397712293 1104 10-11 0.22122908390137042 1104 12-13 0.24427695119990034 1104 14-15 0.22670363651968017 1104 16-17 0.22510071960066114 1104 18-19 0.21324667002375008 1104 20-21 0.1593057062501586 1104 22-23 0.19208062313896335 1104 24-25 0.25412974199945637 1104 26-27 0.26101083505917444 1104 28-29 0.20367265185601724 1104 30-31 0.20773409917485708 1104 32-33 0.26166525833650667 1104 34-35 0.2141250165842763 1104 36-37 0.1703740998903882 1104 38-39 0.1773950576339658 1104 40-41 0.18536441035320195 1104 42-43 0.24574628388796782 1104 44-45 0.1463656217434064 1104 46-47 0.08805910202014644 1104 48-49 0.0819807837668094 1104 50-51 0.16337223546107538 1104 52-53 0.09697793200304261 1104 54-55 0.06883513694734233 1104 56-57 0.14239998298420176 1104 58-59 0.07750370644716753 1104 60-61 0.12643314670174277 1104 62-63 0.08212171344284513 1104 64-65 0.11097161505563236 1104 66-67 0.1695911984837295 1104 68-69 0.1868247890854704 1104 70-71 0.1720773709328256 1104 72-73 0.16959083984635726 1104 74-75 0.1581416118000405 1104 76-77 0.051387675404633626 1104 78-79 0.06024563020474716 1104 80-81 0.07809727472690753 1104 82-83 0.003942425661790594 1104 84-85 0.01846269087035779 1104 86-87 0.07117872044774032 1104 88-89 0.09434712027937664 1104 90-91 0.14032767142390412 1104 92-93 0.09194582615714708 1104 94-95 0.1544086139610883 1104 96-97 0.10409426564293156 1104 98-99 0.18863223349278613 1104 100 0.23596835916483982 1105 1 0.08742600244589482 1105 2 0.03953544027009315 1105 3 0.13112083177341205 1105 4 0.12337949393410241 1105 5 0.06551208884646087 1105 6 0.13193429813750157 1105 7 0.07301803288042663 1105 8 0.0921555508054368 1105 9 0.08196330052295764 1105 10-11 0.11391894421549154 1105 12-13 0.13470056962919585 1105 14-15 0.17627934172887905 1105 16-17 0.13727255592145582 1105 18-19 0.17180516700198467 1105 20-21 0.14752764588122602 1105 22-23 0.23598108081743874 1105 24-25 0.18780380740533076 1105 26-27 0.18945706392368322 1105 28-29 0.2358432991591215 1105 30-31 0.20314166191619165 1105 32-33 0.22534112665509554 1105 34-35 0.2398261080178017 1105 36-37 0.21713240037914971 1105 38-39 0.16748273548311943 1105 40-41 0.20670616512936135 1105 42-43 0.3131063982174851 1105 44-45 0.25897945528939914 1105 46-47 0.19510314873561896 1105 48-49 0.25987168895176893 1105 50-51 0.17062660763208015 1105 52-53 0.23028591494293948 1105 54-55 0.20667377274195076 1105 56-57 0.19403533825314412 1105 58-59 0.18321071855698534 1105 60-61 0.21432646941242695 1105 62-63 0.30027924866659816 1105 64-65 0.27148419369253673 1105 66-67 0.3075498489572581 1105 68-69 0.2790559800783967 1105 70-71 0.2361197238006838 1105 72-73 0.24776053234526785 1105 74-75 0.31329926685896936 1105 76-77 0.2931738499577463 1105 78-79 0.2984798656861045 1105 80-81 0.27872430206131327 1105 82-83 0.2544229690313884 1105 84-85 0.2516348414623266 1105 86-87 0.21809829941198444 1105 88-89 0.2887661971780169 1105 90-91 0.24188346087385781 1105 92-93 0.23404995524368388 1105 94-95 0.29092733958972516 1105 96-97 0.291354321548301 1105 98-99 0.31340431150871595 1105 100 0.28889529926872015 1106 1 -0.06817772061926064 1106 2 -0.036145247778311074 1106 3 -0.01663957221547463 1106 4 0.006316218054053024 1106 5 -0.03826931823725488 1106 6 -0.0857730571957589 1106 7 8.193148574022757E-4 1106 8 -0.033577104061478735 1106 9 -0.08728547951251642 1106 10-11 0.010164196689224525 1106 12-13 0.03498687009399504 1106 14-15 0.13589436226445883 1106 16-17 -0.03689471927858534 1106 18-19 0.052850994394873396 1106 20-21 0.09776545686110438 1106 22-23 0.03912999955987573 1106 24-25 0.03992362859311527 1106 26-27 0.09250204902910752 1106 28-29 0.09329966828194358 1106 30-31 0.09300175719512538 1106 32-33 0.13387488963230965 1106 34-35 0.11427891094184872 1106 36-37 0.16481978036284062 1106 38-39 0.10672830864428562 1106 40-41 0.14354580982313792 1106 42-43 0.19935393027656545 1106 44-45 0.0717659972357012 1106 46-47 0.08285018430874658 1106 48-49 0.129051308686023 1106 50-51 0.07469817888458152 1106 52-53 0.0955365341014982 1106 54-55 0.12782330194961844 1106 56-57 0.05791975168492769 1106 58-59 0.15622622360104543 1106 60-61 0.15247436427330996 1106 62-63 0.06272868244077756 1106 64-65 -0.009583270442639957 1106 66-67 0.07137746594055727 1106 68-69 0.08760597061232289 1106 70-71 0.04996569337787804 1106 72-73 0.03857766709966626 1106 74-75 0.02153320640746159 1106 76-77 -0.024397116625081594 1106 78-79 0.06389005581898033 1106 80-81 0.0721494370011797 1106 82-83 0.026772371509565573 1106 84-85 0.034165876664523864 1106 86-87 0.012672499915034052 1106 88-89 -0.0012994471525722417 1106 90-91 -0.0034783556852069353 1106 92-93 -0.0506609817431638 1106 94-95 -0.08231766947066177 1106 96-97 -0.07031928046700386 1106 98-99 -0.18886831917141222 1106 100 -0.2096500161833923 1107 1 0.031149163515610923 1107 2 0.05546720563417651 1107 3 0.06500789552571717 1107 4 0.08954778939254027 1107 5 0.12434686217625313 1107 6 0.06764506925569691 1107 7 0.08801986032220555 1107 8 0.03900047343912405 1107 9 0.020985181761020044 1107 10-11 0.06701454760149517 1107 12-13 0.11641851012824134 1107 14-15 0.14589882236197127 1107 16-17 0.14763489423089027 1107 18-19 0.11667704033900606 1107 20-21 0.0887719521985062 1107 22-23 0.1130051065765798 1107 24-25 0.06573477678627171 1107 26-27 0.11277857507454314 1107 28-29 0.08591333668650947 1107 30-31 0.1406843007836116 1107 32-33 0.09329364152272746 1107 34-35 0.1459871282870182 1107 36-37 0.1327157934457901 1107 38-39 0.04798882447763475 1107 40-41 0.1037104745724804 1107 42-43 0.13446104951496807 1107 44-45 0.03924470924156509 1107 46-47 0.11324959373031618 1107 48-49 0.08537465037563408 1107 50-51 0.042640330907254054 1107 52-53 -0.01999639628509442 1107 54-55 0.04595744322705286 1107 56-57 0.07941383308427419 1107 58-59 0.10957411633314251 1107 60-61 0.17087764936582062 1107 62-63 0.15313588421661706 1107 64-65 0.15734843880002103 1107 66-67 0.10497635450476395 1107 68-69 0.025067713741464104 1107 70-71 0.08789856634712123 1107 72-73 0.05028209079868162 1107 74-75 0.06056828738129383 1107 76-77 -7.355303683311831E-4 1107 78-79 -0.0032292851118995713 1107 80-81 -0.03275410698204695 1107 82-83 -0.12537385817007873 1107 84-85 -0.06287200340499055 1107 86-87 -0.05521531068973928 1107 88-89 0.01754164521245727 1107 90-91 -0.0015394949068934238 1107 92-93 0.06813961088588272 1107 94-95 -0.010642877224054104 1107 96-97 -0.10022295261395087 1107 98-99 -0.06744088290723127 1107 100 0.02691047965641502 1108 1 -0.12889632528929695 1108 2 -0.10370699145379447 1108 3 -0.12198757956330297 1108 4 -0.133375651990022 1108 5 -0.10814687245996168 1108 6 -0.15225528416142708 1108 7 -0.14894125846802808 1108 8 -0.15131990570527165 1108 9 -0.152387621366735 1108 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-0.0545897409032321 2214 50-51 0.0637938702150862 2214 52-53 0.08097044210597915 2214 54-55 0.08866863501334876 2214 56-57 0.13616377107706512 2214 58-59 0.07179016679830852 2214 60-61 0.13965603642029123 2214 62-63 0.1193106007607696 2214 64-65 4.928121007452546E-4 2214 66-67 0.07968989703538654 2214 68-69 0.05544460693584341 2214 70-71 0.049424401627561565 2214 72-73 -0.04517234694213812 2214 74-75 -0.020513592139899117 2214 76-77 -0.035595741650958246 2214 78-79 0.010516249974699576 2214 80-81 0.044155201365150276 2214 82-83 0.05731499522594419 2214 84-85 0.049756138227358804 2214 86-87 0.06594359994247867 2214 88-89 0.07781564594864321 2214 90-91 0.05083360360477229 2214 92-93 0.0071248413235949215 2214 94-95 0.09514867431459706 2214 96-97 0.11570839554107692 2214 98-99 0.05377016116764466 2214 100 0.05738863811364325 2215 1 0.026768197820540962 2215 2 -0.05074371882041717 2215 3 -0.08232669599163955 2215 4 -0.009947539016785356 2215 5 -0.05736535541191756 2215 6 -0.008319111332546925 2215 7 0.005530209274105857 2215 8 -0.06400068377951129 2215 9 -0.017562159354412188 2215 10-11 -0.028637882945965032 2215 12-13 -0.01954722377360696 2215 14-15 -0.12599971519326658 2215 16-17 -0.07518840857982667 2215 18-19 -0.05589641197815354 2215 20-21 -0.0883207107246804 2215 22-23 -0.07276762810968762 2215 24-25 -0.06654043931848719 2215 26-27 -0.16892276931838524 2215 28-29 -0.12678111876056875 2215 30-31 -0.12356447792356562 2215 32-33 -0.19234255689755742 2215 34-35 -0.1327894819167028 2215 36-37 -0.12260189801953203 2215 38-39 -0.17062686243648528 2215 40-41 -0.1477307674570838 2215 42-43 -0.14656056484386681 2215 44-45 -0.04236747495036042 2215 46-47 -0.04548476634331422 2215 48-49 0.03690784857067797 2215 50-51 -0.061036517730094886 2215 52-53 -0.04304306559239279 2215 54-55 -0.046329578061104826 2215 56-57 -0.08592583776857765 2215 58-59 -0.05032336496885392 2215 60-61 0.021452297734448678 2215 62-63 0.0548145502308941 2215 64-65 0.039192222050637326 2215 66-67 -0.02295781606460423 2215 68-69 0.08755689879548356 2215 70-71 0.019338410394432515 2215 72-73 -0.07964265115709424 2215 74-75 -0.035023719266881415 2215 76-77 -0.04482882238352204 2215 78-79 -0.07939586246303065 2215 80-81 -0.11676553937018497 2215 82-83 -0.12008995127569477 2215 84-85 -0.16060773816115415 2215 86-87 -0.08105545918652268 2215 88-89 -2.2034054365605016E-4 2215 90-91 -0.08647142093810345 2215 92-93 -0.1346091328717378 2215 94-95 -0.15602464287562867 2215 96-97 -0.1671092578245421 2215 98-99 -0.16320344338938142 2215 100 -0.16950043392498415 2216 1 0.050355510222633626 2216 2 0.049101912459274644 2216 3 -0.02166654521487743 2216 4 -0.105553062081583 2216 5 -0.06304333057323674 2216 6 -0.048205365959688606 2216 7 -0.014414400435306618 2216 8 -0.08879671894079877 2216 9 -0.16530793416993106 2216 10-11 -0.2142181340125049 2216 12-13 -0.1778985430346367 2216 14-15 -0.2519923288709336 2216 16-17 -0.20631535725329542 2216 18-19 -0.22236498874986665 2216 20-21 -0.13577815954995032 2216 22-23 -0.14224290274489704 2216 24-25 -0.18277016506100807 2216 26-27 -0.16281256174021053 2216 28-29 -0.18981772110931416 2216 30-31 -0.18776322159310155 2216 32-33 -0.29875198037385786 2216 34-35 -0.23981740654827632 2216 36-37 -0.275606757142036 2216 38-39 -0.2341823033280832 2216 40-41 -0.151937322212369 2216 42-43 -0.1320028675747551 2216 44-45 -0.09278626602804962 2216 46-47 -0.07580637497085974 2216 48-49 -0.10038076392107342 2216 50-51 -0.08354866553636242 2216 52-53 -0.044287536897343216 2216 54-55 -0.10573502330012019 2216 56-57 -0.11423754570238742 2216 58-59 -0.08975335526201178 2216 60-61 -0.11125040920586571 2216 62-63 -0.09885179952615175 2216 64-65 -0.1254611325113686 2216 66-67 -0.19211682724956347 2216 68-69 -0.1816771444249099 2216 70-71 -0.2890612150155434 2216 72-73 -0.22652609920981348 2216 74-75 -0.16990543405677272 2216 76-77 -0.20564579353524692 2216 78-79 -0.16103057447200797 2216 80-81 -0.11260690591108613 2216 82-83 -0.19335628221771373 2216 84-85 -0.18676523986805194 2216 86-87 -0.23042536968687344 2216 88-89 -0.2541489318257426 2216 90-91 -0.2571299895423813 2216 92-93 -0.30398808028517266 2216 94-95 -0.265824106077293 2216 96-97 -0.2960279783458901 2216 98-99 -0.2909165484812668 2216 100 -0.28008590901146846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40406.0 3 6216.0 4 4079.0 5 3538.0 6 2823.0 7 2992.0 8 3415.0 9 3792.0 10 4051.0 11 4156.0 12 4636.0 13 4937.0 14 5260.0 15 5468.0 16 5380.0 17 5193.0 18 5190.0 19 5026.0 20 5189.0 21 5514.0 22 6599.0 23 9060.0 24 13953.0 25 18685.0 26 19720.0 27 17396.0 28 18278.0 29 22819.0 30 27411.0 31 33233.0 32 43086.0 33 58096.0 34 85552.0 35 135708.0 36 258312.0 37 514092.0 38 759573.0 39 308069.0 40 550.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41821393672119 18.78723759224945 12.897169003780668 23.897379467248694 2 15.746984350130482 19.443286391804218 40.12769550217741 24.68203375588789 3 18.81582535312546 24.85890130666015 28.98325317777567 27.342020162438725 4 12.505413371512414 16.035537572030158 37.62446714649068 33.834581909966744 5 13.509594913738287 38.0399891087165 34.68966709677002 13.760748880775186 6 32.12562894256853 36.87046661428424 17.45348703333017 13.550417409817058 7 28.92328413065003 32.01639164791891 21.33657212733098 17.72375209410008 8 26.207316127280837 36.262305042239504 19.399872667455224 18.13050616302444 9 25.73882531259392 17.153520862755997 20.010131630400707 37.09752219424937 10-11 24.709205618850152 26.795459755778573 27.717364848555846 20.777969776815425 12-13 25.080722599055605 24.00457613212612 27.998229011013336 22.916472257804934 14-15 22.37198175550912 25.907831469121025 24.874661026295026 26.845525749074827 16-17 22.489457246379594 29.342555116297998 25.483237733716475 22.684749903605937 18-19 22.541267450566615 28.341907596701066 27.181609906733044 21.935215045999268 20-21 23.394395443902226 27.579099424590076 26.824779002718397 22.2017261287893 22-23 23.203001902850602 27.23964667289976 26.83118315861081 22.726168265638833 24-25 22.909258590103455 27.330099077774626 26.874449980296745 22.886192351825173 26-27 22.73085419430001 27.363076457591433 26.979941195634893 22.926128152473655 28-29 22.777634042188787 27.538197143694454 26.946040178776105 22.738128635340658 30-31 22.89390366446674 27.676020137651047 26.693718850517833 22.73635734736438 32-33 22.725577019444344 27.477503043322738 26.991480312171092 22.80543962506183 34-35 22.909006814592196 27.24584641112956 27.04811417168571 22.797032602592534 36-37 23.030231423836746 27.48000779592451 26.7086555968578 22.781105183380948 38-39 22.879823526572302 27.711595529754547 26.776877731519882 22.63170321215327 40-41 22.74049657551539 27.45041011005613 27.035392063597858 22.773701250830623 42-43 22.72800905867577 27.447759306815783 26.953963716012293 22.870267918496157 44-45 22.71419219402767 27.513902301426498 26.997420012406074 22.774485492139753 46-47 22.852076281654156 27.524692197170758 26.88867508993258 22.734556431242506 48-49 22.796825778380185 27.422939679891243 26.95682561678051 22.823408924948062 50-51 22.683766218944054 27.47121934704667 26.964175659600187 22.88083877440909 52-53 22.767668397885206 27.422061330425052 26.994283160989184 22.815987110700554 54-55 22.749299204698083 27.64447331725802 26.870401804119908 22.73582567392399 56-57 22.607815820023195 27.482507495633314 26.960201787720916 22.949474896622576 58-59 22.64715085071799 27.118684809445675 27.191686312500973 23.042478027335367 60-61 22.837295106719534 27.462188581099955 26.89192429252748 22.80859201965303 62-63 22.600248290227277 27.50226333550005 26.908988663729144 22.98849971054353 64-65 22.652734862858427 27.268276099862504 27.06660711937555 23.012381917903515 66-67 22.536964320341283 27.26128724710749 27.063881617219316 23.13786681533191 68-69 22.720069937118403 27.40028597785419 26.968511531524726 22.911132553502682 70-71 22.745413850725264 27.217673154264403 27.014050772486474 23.022862222523855 72-73 22.622497973574973 27.285083310155432 27.099155152956968 22.993263563312627 74-75 22.608588161011152 27.41738711359759 26.98406863289666 22.9899560924946 76-77 22.673461172324906 27.328528379799643 26.89611891906522 23.101891528810228 78-79 22.64891049867669 27.324780850453145 27.003906203448587 23.02240244742158 80-81 22.6261556746118 27.27119399982387 27.101322052166243 23.001328273398094 82-83 22.560608707859213 27.317320481577273 27.11929248012701 23.002778330436506 84-85 22.584372107829214 27.239436494544588 27.019434579728074 23.15675681789812 86-87 22.69395318473441 27.314035389281898 26.97932322740006 23.01268819858363 88-89 22.517066325077593 27.251019899502683 26.986324722105408 23.245589053314315 90-91 22.52430263112716 27.17328033511677 27.045369624185284 23.257047409570784 92-93 22.685732197916682 27.29823107191201 26.9470993917757 23.06893733839561 94-95 22.453343889867345 27.08875971497358 27.130002908432836 23.327893486726236 96-97 22.62118372260238 27.005368438911287 27.134893641086126 23.238554197400205 98-99 22.493919134946832 27.168091207219604 27.08277833174146 23.255211326092105 100 22.54649656468923 27.014036523479234 26.990002072214033 23.4494648396175 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2689.0 1 1696.0 2 651.5 3 500.5 4 372.5 5 311.0 6 269.0 7 259.0 8 271.0 9 292.5 10 289.5 11 279.0 12 275.5 13 279.0 14 306.0 15 345.0 16 413.0 17 548.0 18 744.0 19 990.0 20 1419.5 21 2081.0 22 2982.5 23 4435.5 24 6386.0 25 8963.0 26 12350.0 27 16334.0 28 21386.5 29 27314.5 30 33000.5 31 38079.5 32 43429.5 33 49249.0 34 54731.5 35 59676.0 36 64708.5 37 70025.5 38 74775.0 39 77529.5 40 79850.0 41 82817.0 42 84953.5 43 86162.0 44 85492.5 45 84956.0 46 85690.5 47 84985.5 48 84516.5 49 84039.5 50 81729.5 51 79153.0 52 74917.0 53 71626.5 54 69724.5 55 66663.5 56 62648.0 57 57413.5 58 53069.5 59 50148.5 60 45967.5 61 40248.5 62 35539.0 63 31200.5 64 26307.0 65 21630.5 66 17841.0 67 14686.0 68 11833.5 69 9400.0 70 7235.5 71 5691.5 72 4694.0 73 3746.5 74 3024.0 75 2355.0 76 1658.5 77 1288.5 78 986.0 79 670.5 80 487.0 81 331.5 82 224.5 83 150.5 84 104.0 85 75.0 86 51.5 87 40.5 88 34.5 89 27.5 90 15.5 91 11.0 92 12.0 93 9.5 94 5.5 95 5.0 96 4.0 97 3.0 98 1.5 99 1.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07927496505483657 2 0.08206008348089752 3 0.08306918435990511 4 0.08480483787179817 5 0.08391682909827149 6 0.08347282471150815 7 0.08258481593798148 8 0.08254445190282116 9 0.08222153962153873 10-11 0.08216099356879827 12-13 0.0807280703206075 14-15 0.07935569312515717 16-17 0.07820531812308851 18-19 0.07768058566600457 20-21 0.07723658127924121 22-23 0.07897223479113429 24-25 0.07965842338885945 26-27 0.07897223479113429 28-29 0.07437073478285966 30-31 0.0803446119865846 32-33 0.07877041461533275 34-35 0.07600547820685195 36-37 0.07267544530612689 38-39 0.07424964267737874 40-41 0.0766109387342565 42-43 0.08218117558637843 44-45 0.08288754620168375 46-47 0.08583412076838591 48-49 0.0888210593702484 50-51 0.08970906814377508 52-53 0.08966870410861479 54-55 0.08999161638989721 56-57 0.08793305059672171 58-59 0.08724686199899655 60-61 0.08514793217066076 62-63 0.08573321068048516 64-65 0.08514793217066076 66-67 0.08448192559051575 68-69 0.08421955936197377 70-71 0.08224172163911889 72-73 0.08212062953363797 74-75 0.08161607909413418 76-77 0.08159589707655401 78-79 0.08199953742815706 80-81 0.08310954839506542 82-83 0.08256463392040131 84-85 0.08153535102381357 86-87 0.0824435418149204 88-89 0.07927496505483657 90-91 0.0766109387342565 92-93 0.07612657031233287 94-95 0.07640911855845499 96-97 0.07560183785524892 98-99 0.07505692338058481 100 0.0742698246949589 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2477453.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.57085460449766 #Duplication Level Percentage of deduplicated Percentage of total 1 78.4699814415104 49.88403781035887 2 12.790628987085986 16.26222431276233 3 3.8747650409365515 7.3896637513190395 4 1.6549332775036254 4.20822091057311 5 0.8956956348715868 2.847006848715243 6 0.5350399957590585 2.040776986679409 7 0.357149343444114 1.5893002288924298 8 0.2551748108674644 1.2977344640308617 9 0.18119888572849308 1.0367071217228718 >10 0.9307517639069309 10.347729226511598 >50 0.043128859788446515 1.8476913605247767 >100 0.011361207895355743 1.09470232353447 >500 6.358356732464434E-5 0.02890074125245632 >1k 1.2716713464928708E-4 0.12530391312253628 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 4.036403516030375E-5 0.0 0.0 0.0 5 0.0 4.036403516030375E-5 0.0 0.0 0.0 6 0.0 4.036403516030375E-5 0.0 0.0 0.0 7 0.0 4.036403516030375E-5 0.0 0.0 0.0 8 0.0 4.036403516030375E-5 0.0 0.0 0.0 9 0.0 4.036403516030375E-5 0.0 0.0 0.0 10-11 0.0 4.036403516030375E-5 0.0 0.0 0.0 12-13 0.0 4.036403516030375E-5 0.0 0.0 0.0 14-15 0.0 4.036403516030375E-5 0.0 0.0 0.0 16-17 0.0 4.036403516030375E-5 0.0 0.0 0.0 18-19 0.0 4.036403516030375E-5 0.0 0.0 0.0 20-21 0.0 4.036403516030375E-5 0.0 0.0 0.0 22-23 0.0 4.036403516030375E-5 0.0 0.0 0.0 24-25 0.0 1.2109210548091125E-4 0.0 6.0546052740455625E-5 0.0 26-27 0.0 1.61456140641215E-4 0.0 2.220021933816706E-4 0.0 28-29 0.0 1.61456140641215E-4 0.0 0.0013320131602900238 0.0 30-31 0.0 1.61456140641215E-4 0.0 0.004702410096175387 0.0 32-33 0.0 1.61456140641215E-4 0.0 0.01313849344467887 0.0 34-35 0.0 1.8163815822136686E-4 0.0 0.024662425482945588 0.0 36-37 0.0 2.421842109618225E-4 0.0 0.0457929978893646 0.0 38-39 0.0 2.8254824612212624E-4 0.0 0.0816968071644548 0.0 40-41 0.0 3.027302637022781E-4 0.0 0.1260165177704683 0.0 42-43 0.0 3.2291228128243E-4 0.0 0.17291952662674126 0.0 44-45 0.0 3.2291228128243E-4 0.0 0.22291038417277745 0.0 46-47 0.0 3.2291228128243E-4 0.0 0.2884010312203703 0.0 48-49 0.0 3.2291228128243E-4 0.0 0.3652945182007489 0.0 50-51 0.0 3.2291228128243E-4 0.0 0.4494737135275624 0.0 52-53 0.0 3.2291228128243E-4 0.0 0.5370031237726811 0.0 54-55 0.0 3.2291228128243E-4 0.0 0.6291340340260744 0.0 56-57 0.0 3.2291228128243E-4 0.0 0.739368214048864 0.0 58-59 0.0 3.2291228128243E-4 0.0 0.8647792712919277 0.0 60-61 0.0 3.2291228128243E-4 0.0 0.999958425043785 0.0 62-63 0.0 3.2291228128243E-4 0.0 1.1442396687242906 0.0 64-65 0.0 3.2291228128243E-4 0.0 1.2892272830201017 0.0 66-67 0.0 3.632763164427337E-4 0.0 1.4484634017275 0.0 68-69 0.0 3.632763164427337E-4 0.0 1.6364992595217749 0.0 70-71 0.0 3.8345833402288556E-4 0.0 1.8472197050761407 0.0 72-73 0.0 4.2382236918318936E-4 0.0 2.0735206682023835 0.0 74-75 0.0 4.440043867633412E-4 0.0 2.297097058955306 0.0 76-77 0.0 4.440043867633412E-4 0.0 2.5300984519181595 0.0 78-79 0.0 4.440043867633412E-4 0.0 2.796077261606981 0.0 80-81 0.0 4.440043867633412E-4 0.0 3.0932574704747173 0.0 82-83 0.0 4.440043867633412E-4 0.0 3.395907006106675 0.0 84-85 0.0 4.440043867633412E-4 0.0 3.6912304693570372 0.0 86-87 0.0 4.440043867633412E-4 0.0 4.004132469919712 0.0 88 0.0 4.440043867633412E-4 0.0 4.264056674334488 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4325 0.0 29.776926 1 GTACATG 8965 0.0 28.311237 1 TCAACGC 4545 0.0 27.094604 4 TACATGG 8970 0.0 26.828285 2 ATCAACG 4655 0.0 26.454344 3 CAACGCA 4685 0.0 26.384739 5 AACGCAG 4835 0.0 25.565666 6 ACATGGG 9370 0.0 24.729923 3 TATCAAC 5325 0.0 23.390615 2 GGTATCA 3025 0.0 22.995972 1 CGTTATA 105 0.002335703 22.381855 2 CATGGGG 6415 0.0 22.053892 4 GGACCGT 265 9.058567E-10 21.28302 6 ACGCAGA 5855 0.0 20.951323 7 ACCGCGT 290 2.7866918E-9 19.447884 8 CGCAGAG 6410 0.0 19.28354 8 TATACCG 125 0.0064124647 18.800379 5 GAGTACA 6700 0.0 18.309694 1 AGTACAT 6630 0.0 17.156046 2 GTATAGG 1090 0.0 16.817192 1 >>END_MODULE