FastQCFastQC Report
Wed 25 May 2016
SRR1032912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032912_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477453
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60960.24605915833721165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46710.18854040823377882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26800.10817561422961405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45150.042.7728651
GTATCAA77500.039.2877921
ATCAACG94500.031.8098933
TCAACGC94850.031.6429164
CAACGCA96750.031.109955
TATCAAC98100.030.786422
AACGCAG100500.030.0421146
GTACATG91750.027.757321
TACATGG91700.026.9329012
ACGCAGA116600.025.7724117
CGCAGAG119700.025.0258738
ACATGGG94750.024.8246963
GCAGAGT132650.022.414649
TACACCG4650.021.2391265
CATGGGG64600.020.7541774
CGTATAC2057.501403E-720.6529373
AGAGTAC113400.020.39992110-11
GAGTACT75950.020.34714112-13
AGTACTT76950.019.99116112-13
GAGTACA68200.019.0844541
Produced by FastQC (version 0.11.5)