##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032912_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2477453
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82492785937816	34.0	31.0	34.0	31.0	34.0
2	32.883293850579605	34.0	31.0	34.0	31.0	34.0
3	32.95923030628634	34.0	31.0	34.0	31.0	34.0
4	36.36666447355409	37.0	37.0	37.0	35.0	37.0
5	36.35032551576155	37.0	37.0	37.0	35.0	37.0
6	36.410134117579624	37.0	37.0	37.0	35.0	37.0
7	36.37657747694911	37.0	37.0	37.0	35.0	37.0
8	36.313159926747346	37.0	37.0	37.0	35.0	37.0
9	38.153925422601354	39.0	39.0	39.0	37.0	39.0
10-11	38.165981150802864	39.0	39.0	39.0	37.0	39.0
12-13	38.16072958800833	39.0	39.0	39.0	37.0	39.0
14-15	39.71010852678133	41.0	40.0	41.0	37.0	41.0
16-17	39.71789515280411	41.0	40.0	41.0	37.0	41.0
18-19	39.739631993018634	41.0	40.0	41.0	37.0	41.0
20-21	39.74606279110037	41.0	40.0	41.0	37.0	41.0
22-23	39.70850627640564	41.0	40.0	41.0	37.0	41.0
24-25	39.68648507156342	41.0	40.0	41.0	37.0	41.0
26-27	39.60853243230043	41.0	40.0	41.0	37.0	41.0
28-29	39.54497441525631	41.0	40.0	41.0	36.5	41.0
30-31	39.43468715652729	41.0	39.5	41.0	36.0	41.0
32-33	39.310626881720864	41.0	39.0	41.0	35.5	41.0
34-35	39.216023876134074	41.0	39.0	41.0	35.0	41.0
36-37	39.12651360086347	41.0	39.0	41.0	35.0	41.0
38-39	39.027765208865716	41.0	39.0	41.0	35.0	41.0
40-41	38.92073815325659	41.0	39.0	41.0	35.0	41.0
42-43	38.77372406257555	41.0	39.0	41.0	35.0	41.0
44-45	38.597754629452105	41.0	38.5	41.0	35.0	41.0
46-47	38.35899429777275	41.0	38.0	41.0	35.0	41.0
48-49	38.140317495427766	40.5	38.0	41.0	34.0	41.0
50-51	37.966913196738744	40.0	38.0	41.0	34.0	41.0
52-53	37.81342027477413	40.0	37.0	41.0	34.0	41.0
54-55	37.64878889730703	40.0	37.0	41.0	33.0	41.0
56-57	37.42614027390227	40.0	36.5	41.0	33.0	41.0
58-59	37.24139469043409	40.0	36.0	41.0	33.0	41.0
60-61	37.03637687576717	39.0	35.5	41.0	33.0	41.0
62-63	36.7870789072487	39.0	35.0	41.0	32.5	41.0
64-65	36.49582131326002	39.0	35.0	41.0	32.0	41.0
66-67	36.21599138308578	38.0	35.0	40.5	32.0	41.0
68-69	35.919669515425724	37.0	35.0	40.0	31.5	41.0
70-71	35.59653704833149	37.0	35.0	39.5	31.0	41.0
72-73	35.26322779887248	36.0	35.0	39.0	31.0	41.0
74-75	34.926983882237124	36.0	35.0	39.0	31.0	41.0
76-77	34.59690113192863	35.0	35.0	38.0	31.0	40.0
78-79	34.26618809721113	35.0	34.5	37.0	30.5	39.0
80-81	33.95300395204268	35.0	34.0	37.0	30.0	39.0
82-83	33.6663694528211	35.0	34.0	36.0	30.0	38.5
84-85	33.40629973605958	35.0	34.0	36.0	30.0	37.0
86-87	33.28173329625224	35.0	34.0	36.0	30.0	37.0
88-89	33.11295774329523	35.0	34.0	35.0	30.0	36.5
90-91	32.95332807524502	35.0	34.0	35.0	30.0	36.0
92-93	32.80072033657147	35.0	34.0	35.0	29.5	36.0
94-95	32.709540604806634	35.0	34.0	35.0	29.5	36.0
96-97	32.654776901923064	35.0	34.0	35.0	30.0	36.0
98-99	32.55232894428269	35.0	34.0	35.0	30.0	35.5
100	32.471680794751705	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1934991626106637
1101	2	0.22394695407812293
1101	3	0.17890125441812188
1101	4	0.12540216707161989
1101	5	0.0850629152472635
1101	6	0.10106753825041892
1101	7	0.11654211272912818
1101	8	0.0075326711478211905
1101	9	0.10232053529175289
1101	10-11	0.14115076823600248
1101	12-13	0.17726761192729867
1101	14-15	0.26380072990095016
1101	16-17	0.20868841141871286
1101	18-19	0.27510582547525786
1101	20-21	0.26163554647733633
1101	22-23	0.22469121398448522
1101	24-25	0.26938678578834185
1101	26-27	0.28896341460927033
1101	28-29	0.24856048626422478
1101	30-31	0.23407203280434175
1101	32-33	0.2541914342560858
1101	34-35	0.2949637041856832
1101	36-37	0.2844869105394707
1101	38-39	0.3233511012644712
1101	40-41	0.33689079619133366
1101	42-43	0.35328513328587974
1101	44-45	0.34058693787060434
1101	46-47	0.3646151359904479
1101	48-49	0.30882392850700313
1101	50-51	0.15461890007502177
1101	52-53	0.23310467630034992
1101	54-55	0.26189063093185183
1101	56-57	0.27652024068380854
1101	58-59	0.26986032335619115
1101	60-61	0.28013417650287664
1101	62-63	0.24169594333616828
1101	64-65	0.3076454603899066
1101	66-67	0.3160095472125519
1101	68-69	0.21458544705492955
1101	70-71	0.24224092194958047
1101	72-73	0.28903539936005274
1101	74-75	0.29298043184568456
1101	76-77	0.24768197708760908
1101	78-79	0.23102877438459046
1101	80-81	0.27316386866358044
1101	82-83	0.18374773189301408
1101	84-85	0.23957495709012022
1101	86-87	0.23189055864629893
1101	88-89	0.2168309381721727
1101	90-91	0.20237790163059088
1101	92-93	0.19906884106487155
1101	94-95	0.17665301439284775
1101	96-97	0.1880695216071473
1101	98-99	0.2048963936013024
1101	100	0.15994456979358773
1102	1	0.23116003493533555
1102	2	0.28382534733333387
1102	3	0.21512901632904402
1102	4	0.14317109075006584
1102	5	0.17300175144250574
1102	6	0.17346429592625867
1102	7	0.1312030908328623
1102	8	0.11082945193834348
1102	9	0.22904267720024762
1102	10-11	0.20551889504550758
1102	12-13	0.2313144446279196
1102	14-15	0.31928106478962803
1102	16-17	0.256882135977861
1102	18-19	0.30039309398561187
1102	20-21	0.31392644673552184
1102	22-23	0.35064547825192705
1102	24-25	0.3044709584981291
1102	26-27	0.29356225730796837
1102	28-29	0.3448507753544874
1102	30-31	0.30787153838517156
1102	32-33	0.35304333965042645
1102	34-35	0.33580382500888106
1102	36-37	0.2944900839113558
1102	38-39	0.2847425268919963
1102	40-41	0.2662290110325287
1102	42-43	0.16523424963892808
1102	44-45	0.19769646621350745
1102	46-47	0.2276228265200828
1102	48-49	0.2245164483601627
1102	50-51	0.24217203446536928
1102	52-53	0.22919222218691715
1102	54-55	0.1773674790292077
1102	56-57	0.24948977239598236
1102	58-59	0.22329148273389166
1102	60-61	0.24465666238920392
1102	62-63	0.18931885273052274
1102	64-65	0.252986284447978
1102	66-67	0.34786687084555723
1102	68-69	0.34848802845584004
1102	70-71	0.36598048606148126
1102	72-73	0.4069563314694804
1102	74-75	0.37665578414873124
1102	76-77	0.3851111149098969
1102	78-79	0.3653695553474918
1102	80-81	0.38199269855125806
1102	82-83	0.38927527558565345
1102	84-85	0.36732276413344067
1102	86-87	0.33336167116779336
1102	88-89	0.38544883677315056
1102	90-91	0.42197229769090683
1102	92-93	0.38433793124740134
1102	94-95	0.3726242033103304
1102	96-97	0.32829103080013056
1102	98-99	0.4353515569907742
1102	100	0.49139777822927755
1103	1	0.1816612599827181
1103	2	0.22082223554528468
1103	3	0.17589639759397357
1103	4	0.08717598179174502
1103	5	0.12494360781236935
1103	6	0.12920524757538487
1103	7	0.12964445245739142
1103	8	0.048424913393184
1103	9	0.11336374786268522
1103	10-11	0.15582412775876975
1103	12-13	0.18852560559472664
1103	14-15	0.25269169731657826
1103	16-17	0.24156065823682837
1103	18-19	0.2506031129978581
1103	20-21	0.21530830322735284
1103	22-23	0.21159718836317865
1103	24-25	0.19798766015526326
1103	26-27	0.2655446538269075
1103	28-29	0.23441576537572928
1103	30-31	0.21870790956156583
1103	32-33	0.3543552914474475
1103	34-35	0.30930796364124546
1103	36-37	0.31052669354127715
1103	38-39	0.2944131591445185
1103	40-41	0.3059782536681439
1103	42-43	0.35150106450049634
1103	44-45	0.33900587186352027
1103	46-47	0.37773237182701536
1103	48-49	0.3745583759306754
1103	50-51	0.3626551772950677
1103	52-53	0.3598501191304706
1103	54-55	0.3607304665896791
1103	56-57	0.39983403586578703
1103	58-59	0.3567472445730999
1103	60-61	0.338089737601166
1103	62-63	0.35816445712026024
1103	64-65	0.36967597316245104
1103	66-67	0.3247116458317194
1103	68-69	0.3473035364731487
1103	70-71	0.31988013127800485
1103	72-73	0.3110095840579348
1103	74-75	0.3087131471637363
1103	76-77	0.3440670726652044
1103	78-79	0.34293007207811854
1103	80-81	0.33994277577421883
1103	82-83	0.3545745698302838
1103	84-85	0.34514887296521835
1103	86-87	0.34135062668340055
1103	88-89	0.27239030436008704
1103	90-91	0.3088369234759085
1103	92-93	0.37446947709405976
1103	94-95	0.3242769119076243
1103	96-97	0.311704102749367
1103	98-99	0.33737406675989945
1103	100	0.35357804238821444
1104	1	0.086415941495666
1104	2	0.10996305366815307
1104	3	0.10044418167071711
1104	4	0.052768477632113786
1104	5	0.03656664089588446
1104	6	0.07691216822917113
1104	7	0.050450003922229314
1104	8	-0.05101836574956309
1104	9	-0.06787427573257077
1104	10-11	0.0531386785810426
1104	12-13	0.09753573892853495
1104	14-15	0.1453101412783937
1104	16-17	0.14185715786512532
1104	18-19	0.1799686198613486
1104	20-21	0.1402392511785422
1104	22-23	0.12378149737318012
1104	24-25	0.13770231381300135
1104	26-27	0.1968127128706314
1104	28-29	0.17164753776495445
1104	30-31	0.1933083496490653
1104	32-33	0.2793522805319384
1104	34-35	0.28897573399087406
1104	36-37	0.314220279782667
1104	38-39	0.291262643293976
1104	40-41	0.24924678266528844
1104	42-43	0.2866872938628262
1104	44-45	0.30439877017152384
1104	46-47	0.36967244215198036
1104	48-49	0.40909026001492066
1104	50-51	0.3802076722568444
1104	52-53	0.3961863413211475
1104	54-55	0.40148724343448094
1104	56-57	0.4209377527285767
1104	58-59	0.3823509718285223
1104	60-61	0.4010486810963414
1104	62-63	0.3721385324375248
1104	64-65	0.4152613445207365
1104	66-67	0.32688724419313786
1104	68-69	0.39903946746370167
1104	70-71	0.38305547741536117
1104	72-73	0.35676739205838004
1104	74-75	0.3738225182089181
1104	76-77	0.33689405477262824
1104	78-79	0.37067925168656046
1104	80-81	0.3543680461780241
1104	82-83	0.31675787643022346
1104	84-85	0.35422319002545777
1104	86-87	0.30144497705608586
1104	88-89	0.3238483245324062
1104	90-91	0.3329910625965624
1104	92-93	0.32639624927028166
1104	94-95	0.30639236861374997
1104	96-97	0.33292692951330594
1104	98-99	0.3098310077198647
1104	100	0.35070974919329956
1105	1	0.07580632795728803
1105	2	0.1531378946280597
1105	3	0.1104722822659241
1105	4	0.030927744685989467
1105	5	0.02623123379409975
1105	6	0.04872534052049815
1105	7	0.022898898598882056
1105	8	0.016191290452717055
1105	9	0.10264629933554659
1105	10-11	0.04847063530566942
1105	12-13	0.08234826365036696
1105	14-15	0.08485555597452077
1105	16-17	0.15645877626468518
1105	18-19	0.11114294787787315
1105	20-21	0.08300485034042282
1105	22-23	0.14582811849864896
1105	24-25	0.10026713986925984
1105	26-27	0.09065766510637019
1105	28-29	0.1079899642355926
1105	30-31	0.11452131571474666
1105	32-33	0.16011484295984957
1105	34-35	0.14536354130365936
1105	36-37	0.14988381735587808
1105	38-39	0.10186702256965674
1105	40-41	0.15936892766757893
1105	42-43	0.1340702666267788
1105	44-45	0.12793163469968505
1105	46-47	0.16101785803999746
1105	48-49	0.12881390961284467
1105	50-51	0.12717774139291294
1105	52-53	0.15455069669584276
1105	54-55	0.11476894448799868
1105	56-57	0.18589893051092332
1105	58-59	0.15268624394520458
1105	60-61	0.19326668950175474
1105	62-63	0.20069646865318447
1105	64-65	0.15780059951642045
1105	66-67	0.14916327536980845
1105	68-69	0.1369201568613363
1105	70-71	0.14468298990632888
1105	72-73	0.14485582568050148
1105	74-75	0.12620071515691933
1105	76-77	0.17145574055847135
1105	78-79	0.07528019013457765
1105	80-81	0.1236023640146584
1105	82-83	0.1468577661229915
1105	84-85	0.1824813587960321
1105	86-87	0.14457390872152587
1105	88-89	0.12170809985545361
1105	90-91	0.09649300117647641
1105	92-93	0.14411010713512695
1105	94-95	0.10296727245661685
1105	96-97	0.14387149996378668
1105	98-99	0.1274958616850128
1105	100	0.12830908656005136
1106	1	0.06320447106115523
1106	2	0.10782591985032042
1106	3	0.08512252046327262
1106	4	0.004187822191894952
1106	5	0.0355473968269564
1106	6	0.042810782470276365
1106	7	0.04326448226932911
1106	8	0.036898425554852565
1106	9	0.06959926646702286
1106	10-11	0.06555941163198753
1106	12-13	0.05892958109615165
1106	14-15	0.06976238275308333
1106	16-17	0.08121777772903016
1106	18-19	0.10266095853396706
1106	20-21	0.09499778748954668
1106	22-23	0.08659367280494479
1106	24-25	0.09164180710775582
1106	26-27	0.05841466903575565
1106	28-29	0.07721633478799106
1106	30-31	0.08333349720064831
1106	32-33	0.12387561786432144
1106	34-35	0.08307097391100626
1106	36-37	0.03900401332293768
1106	38-39	0.028751833962950002
1106	40-41	0.037570682561927526
1106	42-43	0.043255836465164066
1106	44-45	0.13040865532027368
1106	46-47	0.14208493580476755
1106	48-49	0.05206258919200479
1106	50-51	0.060233660780596665
1106	52-53	0.09460687495169395
1106	54-55	0.12539413042875225
1106	56-57	0.10293129675914514
1106	58-59	-0.007412125958083493
1106	60-61	-0.03725657192805443
1106	62-63	-0.03289921018028252
1106	64-65	-0.034002166122888866
1106	66-67	-0.02951448785468358
1106	68-69	0.019501420215263465
1106	70-71	0.06668292035966061
1106	72-73	0.02147037098049509
1106	74-75	0.030885069860396186
1106	76-77	0.060943374773884784
1106	78-79	-0.0016419060021064524
1106	80-81	-9.982255865708112E-4
1106	82-83	-0.002625077099494888
1106	84-85	-0.0243060099829151
1106	86-87	-0.06716827403939618
1106	88-89	-0.09225107624830997
1106	90-91	-0.06844813529340854
1106	92-93	-0.14971453479610375
1106	94-95	-0.05821409367184316
1106	96-97	-0.16371024635313347
1106	98-99	-0.13775243925116598
1106	100	-0.10340883213989116
1107	1	-0.024097580071043012
1107	2	-0.01096852092010181
1107	3	0.00811970598795142
1107	4	0.024361817260150076
1107	5	0.023288793196336144
1107	6	0.01595586892952383
1107	7	-0.035950950693354855
1107	8	0.030957780737537632
1107	9	-0.022639651215982326
1107	10-11	0.012075152700255387
1107	12-13	0.037808199798831765
1107	14-15	0.05155185589765665
1107	16-17	0.06253048182976073
1107	18-19	0.05353375347713296
1107	20-21	0.030542714000475257
1107	22-23	0.038348746806676104
1107	24-25	0.07637699522646813
1107	26-27	0.07238348936559902
1107	28-29	0.05739607159097204
1107	30-31	0.06768009291587873
1107	32-33	0.04117310636803495
1107	34-35	0.0734874034747861
1107	36-37	0.0719682351384563
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2216	16-17	-0.3613119668985547
2216	18-19	-0.28184853305769053
2216	20-21	-0.2338626789468563
2216	22-23	-0.3025263027075056
2216	24-25	-0.32170983543672804
2216	26-27	-0.3455803330952989
2216	28-29	-0.33497635162615325
2216	30-31	-0.3271862705063313
2216	32-33	-0.4435376242930289
2216	34-35	-0.41060328458632966
2216	36-37	-0.40535727966238255
2216	38-39	-0.3945540857330698
2216	40-41	-0.4034946387948395
2216	42-43	-0.43234975841328094
2216	44-45	-0.42914821660838953
2216	46-47	-0.45471593882229655
2216	48-49	-0.5268366251552905
2216	50-51	-0.44612598318396834
2216	52-53	-0.4642362889979168
2216	54-55	-0.46840843054000914
2216	56-57	-0.6211057682598735
2216	58-59	-0.6121586587962753
2216	60-61	-0.6132301386709003
2216	62-63	-0.5339717950062166
2216	64-65	-0.5672603871562742
2216	66-67	-0.5626853653576589
2216	68-69	-0.6057917921731573
2216	70-71	-0.6234653880141323
2216	72-73	-0.5484342473753188
2216	74-75	-0.5968971038425224
2216	76-77	-0.6493363471945628
2216	78-79	-0.6641147032751107
2216	80-81	-0.6559666611714263
2216	82-83	-0.6446492642009432
2216	84-85	-0.6825622944048106
2216	86-87	-0.6166610386393288
2216	88-89	-0.6240980014581012
2216	90-91	-0.6065713439277829
2216	92-93	-0.6219468503969736
2216	94-95	-0.5698350798289056
2216	96-97	-0.5907735373256457
2216	98-99	-0.5838866334122237
2216	100	-0.5709739610899867
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9.0
3	4.0
4	7.0
5	15.0
6	70.0
7	159.0
8	311.0
9	608.0
10	981.0
11	1492.0
12	2318.0
13	3635.0
14	4254.0
15	5549.0
16	7294.0
17	10878.0
18	13773.0
19	7815.0
20	5080.0
21	4886.0
22	4997.0
23	5350.0
24	6083.0
25	7081.0
26	8998.0
27	11316.0
28	14800.0
29	18933.0
30	23864.0
31	30734.0
32	38791.0
33	51361.0
34	72559.0
35	116673.0
36	232054.0
37	510826.0
38	849442.0
39	402957.0
40	1496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.229666199652144	18.87743754740278	13.193382359767487	23.699513893177592
2	16.029908244141946	19.606860942164587	39.75222043364048	24.61101038005299
3	18.959035331461518	24.8795133064132	29.341839922630186	26.819611439495095
4	12.6747673737965	16.124982338574572	37.408029388611915	33.79222089901702
5	13.06791968002467	37.74249316129022	34.86165765400669	14.327929504678416
6	31.98546032873704	36.95857687215423	17.144581328510995	13.911381470597734
7	28.594822620690547	32.18386304199794	21.300492324508735	17.920822012802777
8	26.39396188341895	35.59948647551785	19.292375875344195	18.714175765719006
9	26.069154033258712	16.157576452333995	20.249363654050615	37.52390586035668
10-11	24.898716544774008	26.198075200619346	27.739880433655053	21.1633278209516
12-13	25.658570569969353	23.398443318106647	28.378465106648008	22.564521005275992
14-15	22.642665903516075	25.100980888002134	25.807796648891664	26.448556559590124
16-17	22.062699070375906	28.80890172285811	26.292264676665916	22.836134530100065
18-19	22.02407717612868	27.27459686371067	28.96022119518053	21.741104764980122
20-21	22.897124587227285	26.432166422531527	28.864745365502394	21.805963624738794
22-23	22.616200746938127	26.235207809650863	28.83334561075462	22.315245832656394
24-25	22.29275414499783	26.398901448428298	28.883498476655777	22.424845929918096
26-27	22.107953274823256	26.386351397936618	29.04362582721308	22.462069500027045
28-29	22.223998394985703	26.538463323949895	29.00683887065284	22.23069941041156
30-31	22.38696790258223	26.67372200539021	28.667517936412327	22.27179215561523
32-33	22.192495450148648	26.51866932794618	28.9897997742434	22.299035447661772
34-35	22.29977557640647	26.37384000537793	28.91757656433863	22.408807853876965
36-37	22.523466718568162	26.572167175586696	28.535715525950884	22.368650579894254
38-39	22.43095571389257	26.821668143630774	28.538843348321034	22.208532794155627
40-41	22.3204600178185	26.618123500148215	28.672723557173885	22.388692924859395
42-43	22.3150464040122	26.61767747272633	28.512615686882786	22.554660436378676
44-45	22.355432106972188	26.749694751668386	28.371698413234007	22.52317472812542
46-47	22.524060756567582	26.89995615213794	28.072310580266844	22.503672511027638
48-49	22.612756146868147	26.92686396319305	27.855261549403775	22.60511834053503
50-51	22.50791825831657	26.98358558942656	27.804902197512426	22.703593954744438
52-53	22.614115680758157	26.98614908523994	27.704799651504587	22.69493558249732
54-55	22.610947001131763	27.192312984840928	27.55724131158393	22.639498702443383
56-57	22.468511319682076	27.014121975630083	27.706988097070756	22.810378607617082
58-59	22.48780301559594	26.66452399186376	27.946048229576228	22.901624762964072
60-61	22.626811338021483	26.945578547374076	27.728537172201605	22.699072942402836
62-63	22.3789138594862	26.957659521471445	27.814020350564316	22.849406268478038
64-65	22.427690858312953	26.771284863930816	27.896089249725424	22.904935028030803
66-67	22.299887142112137	26.813233919365544	27.836301167489825	23.050577771032497
68-69	22.513435692634705	26.93582654257937	27.722906158127543	22.827831606658382
70-71	22.57746616736345	26.74652692634874	27.715041963468522	22.960964942819288
72-73	22.42086933637086	26.9014588773228	27.741938999448223	22.935732786858114
74-75	22.441475176320196	27.07387385351004	27.549422733751154	22.935228236418613
76-77	22.48878887565844	26.966457446164405	27.464247512563322	23.080506165613837
78-79	22.478287176386395	27.004467895051892	27.54881727322375	22.968427655337962
80-81	22.466053225410295	26.968616249526477	27.55799373104019	23.007336794023033
82-83	22.365122349642714	27.001221821317007	27.61049773375759	23.02315809528269
84-85	22.40625755564283	26.95346793662685	27.409500805867964	23.230773701862358
86-87	22.454146636713652	26.977917285838522	27.449110178650464	23.118825898797365
88-89	22.211117587319833	27.01007570705937	27.43525181598454	23.343554889636252
90-91	22.24085248945488	26.909846818301077	27.48897051403661	23.36033017820743
92-93	22.351103410486463	27.078901241507086	27.348151765991606	23.22184358201485
94-95	22.07236935049628	26.850829286801243	27.496780938311073	23.580020424391407
96-97	22.190238138245107	26.813625717598455	27.49896819206058	23.497167952095857
98-99	22.069403954263503	26.93155570086611	27.443815774469943	23.55522457040044
100	22.10564640378647	26.79388872362059	27.362981255345716	23.73748361724723
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	59.0
1	58.5
2	272.0
3	486.5
4	298.5
5	173.5
6	455.5
7	1075.5
8	1908.0
9	2190.5
10	1617.0
11	1105.0
12	1087.5
13	1306.5
14	1514.5
15	1675.0
16	1850.0
17	2008.0
18	2302.5
19	2615.0
20	2926.0
21	3485.5
22	4324.5
23	5681.0
24	7558.0
25	9869.5
26	13005.0
27	16909.0
28	21851.0
29	27676.5
30	33262.5
31	38512.0
32	43562.0
33	48945.5
34	54355.0
35	58890.5
36	63921.0
37	69299.5
38	74097.5
39	76933.0
40	79276.0
41	82601.0
42	84636.0
43	85668.0
44	85466.5
45	85218.5
46	85457.0
47	84398.0
48	84137.5
49	83581.5
50	80960.0
51	77938.5
52	73877.5
53	71052.0
54	69151.0
55	66264.0
56	62112.5
57	56559.0
58	52137.0
59	49350.0
60	45440.5
61	39886.5
62	35037.0
63	30487.0
64	25357.5
65	20885.0
66	17428.0
67	14141.0
68	11279.5
69	9004.5
70	6892.0
71	5311.0
72	4325.0
73	3424.0
74	2747.0
75	2106.0
76	1463.5
77	1104.5
78	804.0
79	539.5
80	378.0
81	211.0
82	118.5
83	72.5
84	28.5
85	15.5
86	10.5
87	7.0
88	5.0
89	1.5
90	0.5
91	2.0
92	2.0
93	1.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.650964922442525E-4
2	3.632763164427337E-4
3	4.036403516030375E-5
4	0.012230302653572035
5	4.8436842192364494E-4
6	0.0037538552699082485
7	8.880087735266824E-4
8	0.14405924148712407
9	0.0010898289493282013
10-11	0.0
12-13	1.61456140641215E-4
14-15	1.61456140641215E-4
16-17	0.0
18-19	1.2109210548091124E-4
20-21	0.0
22-23	2.220021933816706E-4
24-25	3.632763164427337E-4
26-27	0.006417881590488296
28-29	0.00873881361220576
30-31	0.014894328974152084
32-33	0.018123451786976382
34-35	0.026781537328861536
36-37	0.039032022000013725
38-39	0.04397661630715093
40-41	0.055500548345417657
42-43	0.07285708346434827
44-45	0.11416967345091915
46-47	0.12060773705898759
48-49	0.11788316468566709
50-51	0.11909408574047621
52-53	0.11362475897625506
54-55	0.12087010328752958
56-57	0.10155591246332424
58-59	0.09499675674977487
60-61	0.09760023701761446
62-63	0.0963287699100649
64-65	0.0
66-67	4.036403516030375E-5
68-69	1.4127412306106312E-4
70-71	2.0182017580151874E-5
72-73	0.0
74-75	0.0
76-77	1.2109210548091124E-4
78-79	0.0
80-81	3.430942988625819E-4
82-83	1.61456140641215E-4
84-85	0.0
86-87	7.063706153053156E-4
88-89	3.632763164427337E-4
90-91	1.2109210548091124E-4
92-93	8.274627207862269E-4
94-95	9.283728086869862E-4
96-97	2.220021933816706E-4
98-99	2.0182017580151874E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2477453.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.192240501716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.02459975924792	47.133078330163144
2	13.555782358914406	16.590173885912186
3	4.172091715971611	7.658989190368554
4	1.7991594035237768	4.403783796854033
5	0.9365634300758038	2.8655207329155328
6	0.5796368415256441	2.1281566206175353
7	0.37997704067598315	1.6276152520722558
8	0.26667918767568005	1.3054957591241976
9	0.20496687904922928	1.1288144301899914
>10	1.0157857958163663	10.884541713542003
>50	0.04992151450084119	2.0629204480741703
>100	0.014109519384172174	1.4369113206484776
>500	4.623523154277718E-4	0.1769890087218915
>1k	1.981509923211283E-4	0.350623350223796
>5k	6.605033077370943E-5	0.24638616057224907
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6096	0.24605915833721165	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4671	0.18854040823377882	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2680	0.10817561422961405	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	4.036403516030375E-5	0.0
9	0.0	0.0	0.0	4.036403516030375E-5	0.0
10-11	0.0	0.0	0.0	4.036403516030375E-5	0.0
12-13	0.0	0.0	0.0	4.036403516030375E-5	0.0
14-15	0.0	0.0	0.0	4.036403516030375E-5	0.0
16-17	0.0	0.0	0.0	4.036403516030375E-5	0.0
18-19	0.0	0.0	0.0	6.0546052740455625E-5	0.0
20-21	0.0	0.0	0.0	8.07280703206075E-5	0.0
22-23	0.0	0.0	0.0	8.07280703206075E-5	0.0
24-25	0.0	0.0	0.0	1.4127412306106312E-4	0.0
26-27	0.0	0.0	0.0	3.8345833402288556E-4	0.0
28-29	0.0	0.0	0.0	0.0014531052657709349	0.0
30-31	0.0	0.0	0.0	0.004863866236816602	0.0
32-33	0.0	0.0	0.0	0.013259585550159781	0.0
34-35	0.0	0.0	0.0	0.024924791711487565	0.0
36-37	0.0	6.054605274045562E-5	0.0	0.046216820258547786	0.0
38-39	0.0	1.2109210548091124E-4	0.0	0.0823224497094395	0.0
40-41	0.0	1.2109210548091124E-4	0.0	0.12686416250883467	0.0
42-43	0.0	2.0182017580151873E-4	0.0	0.17415062969913053	0.0
44-45	0.0	2.0182017580151873E-4	0.0	0.2242222153154873	0.0
46-47	0.0	2.0182017580151873E-4	0.0	0.2898137724509809	0.0
48-49	0.0	2.0182017580151873E-4	0.0	0.36696962565990154	0.0
50-51	0.0	2.0182017580151873E-4	0.0	0.45151209730315767	0.0
52-53	0.0	2.0182017580151873E-4	0.0	0.5393442378119787	0.0
54-55	0.0	2.0182017580151873E-4	0.0	0.6316769682411735	0.0
56-57	0.0	2.0182017580151873E-4	0.0	0.7426780649320088	0.0
58-59	0.0	2.0182017580151873E-4	0.0	0.8684322164739351	0.0
60-61	0.0	2.0182017580151873E-4	0.0	1.0039342825070747	0.0
62-63	0.0	2.0182017580151873E-4	0.0	1.1485989845216036	0.0
64-65	0.0	2.0182017580151873E-4	0.0	1.2941516953096588	0.0
66-67	0.0	2.4218421096182247E-4	0.0	1.4548005552476677	0.0
68-69	0.0	2.4218421096182247E-4	0.0	1.6436638757627289	0.0
70-71	0.0	2.6236622854197434E-4	0.0	1.8553328761433616	0.0
72-73	0.0	2.8254824612212624E-4	0.0	2.0837529511155206	0.0
74-75	0.0	2.8254824612212624E-4	0.0	2.3092264515209777	0.0
76-77	0.0	2.8254824612212624E-4	0.0	2.54341858352106	0.0
78-79	0.0	2.8254824612212624E-4	0.0	2.81052758619437	0.0
80-81	0.0	2.8254824612212624E-4	0.0	3.109786542872862	0.0
82-83	0.0	2.8254824612212624E-4	0.0	3.4146359184210557	0.0
84-85	0.0	2.8254824612212624E-4	0.0	3.712441769833777	0.0
86-87	0.0	2.8254824612212624E-4	0.0	4.027301426101726	0.0
88	0.0	2.8254824612212624E-4	0.0	4.289203468239357	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4515	0.0	42.772865	1
GTATCAA	7750	0.0	39.287792	1
ATCAACG	9450	0.0	31.809893	3
TCAACGC	9485	0.0	31.642916	4
CAACGCA	9675	0.0	31.10995	5
TATCAAC	9810	0.0	30.78642	2
AACGCAG	10050	0.0	30.042114	6
GTACATG	9175	0.0	27.75732	1
TACATGG	9170	0.0	26.932901	2
ACGCAGA	11660	0.0	25.772411	7
CGCAGAG	11970	0.0	25.025873	8
ACATGGG	9475	0.0	24.824696	3
GCAGAGT	13265	0.0	22.41464	9
TACACCG	465	0.0	21.239126	5
CATGGGG	6460	0.0	20.754177	4
CGTATAC	205	7.501403E-7	20.652937	3
AGAGTAC	11340	0.0	20.399921	10-11
GAGTACT	7595	0.0	20.347141	12-13
AGTACTT	7695	0.0	19.991161	12-13
GAGTACA	6820	0.0	19.084454	1
>>END_MODULE