FastQCFastQC Report
Wed 25 May 2016
SRR1032911_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032911_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029031
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9950.043.461471
GTATCAA17300.034.234511
TCAACGC17950.031.6855014
ATCAACG18400.030.9105833
CAACGCA19100.029.7777335
AACGCAG19350.029.635936
CGATATG404.7822087E-429.3749764-65
GTACATG39200.028.0588251
TATCAAC21200.027.7149312
TACATGG38950.027.2734832
ACATGGG39200.025.3009053
ACGCAGA23150.024.5670437
CGCAGAG24450.023.4519188
TACCTGG7100.022.5092092
CATGGGG27550.022.1799934
GTACACG1503.2884935E-521.9354441
GCAGAGT27600.020.605059
ACACCGT1857.1728937E-620.326286
GAGTACA28800.019.4220091
ATGGGGC9850.018.610935
Produced by FastQC (version 0.11.5)