##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032911_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1029031 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.34220057510415 33.0 31.0 34.0 28.0 34.0 2 31.460599340544647 34.0 31.0 34.0 28.0 34.0 3 31.516956243300736 34.0 31.0 34.0 28.0 34.0 4 34.816516703578415 37.0 35.0 37.0 32.0 37.0 5 34.895214041170775 37.0 35.0 37.0 32.0 37.0 6 34.90830985655437 37.0 35.0 37.0 32.0 37.0 7 34.86304008334054 37.0 35.0 37.0 32.0 37.0 8 34.857771048685606 37.0 35.0 37.0 32.0 37.0 9 36.43530175475763 39.0 38.0 39.0 33.0 39.0 10-11 36.436423198134946 39.0 38.0 39.0 32.5 39.0 12-13 36.37759941148518 39.0 38.0 39.0 32.0 39.0 14-15 37.81838642373262 41.0 39.0 41.0 33.0 41.0 16-17 37.83459536204449 41.0 39.0 41.0 33.0 41.0 18-19 37.84041588640187 41.0 39.0 41.0 33.0 41.0 20-21 37.890694740974766 41.0 39.0 41.0 33.0 41.0 22-23 37.8847376804003 41.0 39.0 41.0 32.5 41.0 24-25 37.92620338940226 41.0 39.0 41.0 32.5 41.0 26-27 37.92167680079609 41.0 39.0 41.0 32.0 41.0 28-29 37.91323439235553 41.0 39.0 41.0 32.0 41.0 30-31 37.873227823068504 41.0 39.0 41.0 32.0 41.0 32-33 37.82465202700405 41.0 38.0 41.0 32.0 41.0 34-35 37.749665947867456 41.0 38.0 41.0 32.0 41.0 36-37 37.64970540246115 41.0 38.0 41.0 31.0 41.0 38-39 37.54331453571369 40.0 38.0 41.0 31.0 41.0 40-41 37.4511564763355 40.0 38.0 41.0 31.0 41.0 42-43 37.32325848298059 40.0 38.0 41.0 30.5 41.0 44-45 37.17472262740384 40.0 38.0 41.0 30.0 41.0 46-47 37.03615002852197 40.0 37.0 41.0 30.0 41.0 48-49 36.89457411875833 40.0 37.0 41.0 30.0 41.0 50-51 36.74876315679508 40.0 37.0 41.0 30.0 41.0 52-53 36.58461018181182 40.0 36.0 41.0 30.0 41.0 54-55 36.409500782775254 40.0 36.0 41.0 30.0 41.0 56-57 36.224273612748306 39.5 35.0 41.0 30.0 41.0 58-59 36.01238738191561 39.0 35.0 41.0 29.5 41.0 60-61 35.764642173073504 39.0 35.0 41.0 29.0 41.0 62-63 35.50005442012923 38.5 35.0 40.5 29.0 41.0 64-65 35.216806879481766 38.0 35.0 40.0 28.5 41.0 66-67 34.92214325904662 37.0 34.5 40.0 28.0 41.0 68-69 34.61070317609479 37.0 34.0 40.0 28.0 41.0 70-71 34.28131222480178 36.5 34.0 39.0 28.0 41.0 72-73 33.99914677011674 36.0 34.0 39.0 27.5 41.0 74-75 33.737947642004954 35.5 34.0 39.0 27.0 40.5 76-77 33.426466743956205 35.0 34.0 37.5 27.0 40.0 78-79 33.07228693790566 35.0 34.0 37.0 26.5 39.0 80-81 32.762700054711665 35.0 33.0 37.0 26.0 39.0 82-83 32.45920822599125 35.0 33.0 36.0 26.0 38.5 84-85 32.303883945187266 35.0 33.0 36.0 26.0 37.0 86-87 32.07812592623546 35.0 33.0 36.0 26.0 37.0 88-89 31.8464691539905 35.0 33.0 35.0 25.0 37.0 90-91 31.658191541362697 35.0 33.0 35.0 25.0 36.0 92-93 31.47136092109956 35.0 33.0 35.0 25.0 36.0 94-95 31.293196220522027 35.0 33.0 35.0 24.0 36.0 96-97 31.115297789862502 35.0 33.0 35.0 23.5 36.0 98-99 30.928801950572918 35.0 33.0 35.0 22.0 35.0 100 30.80029950506836 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40655491797669896 1101 2 0.46256406307754006 1101 3 0.47429958088305924 1101 4 0.4334595244813215 1101 5 0.43052831118095014 1101 6 0.4147409582362016 1101 7 0.3269021128744143 1101 8 0.45457157783592805 1101 9 0.4690558547037895 1101 10-11 0.4302222035211969 1101 12-13 0.14877523697339967 1101 14-15 0.23026066654477972 1101 16-17 0.19727562737105586 1101 18-19 0.2974298664534203 1101 20-21 0.32218912502249 1101 22-23 0.22409221723645345 1101 24-25 0.22539473593153758 1101 26-27 0.1564237026949442 1101 28-29 0.2924259412696131 1101 30-31 0.38460590331630584 1101 32-33 0.31378942532872856 1101 34-35 0.3544912452932465 1101 36-37 0.2950869083114256 1101 38-39 0.2772145458739388 1101 40-41 0.36698302424920115 1101 42-43 0.19901130841863335 1101 44-45 0.07607742522732508 1101 46-47 0.18097396379204866 1101 48-49 0.16087679653669085 1101 50-51 0.33265538515080806 1101 52-53 0.23487894413104726 1101 54-55 0.21531631541877516 1101 56-57 0.31093616742757746 1101 58-59 0.11823408672909608 1101 60-61 0.15809783660237997 1101 62-63 0.17450862700424352 1101 64-65 0.124506721162156 1101 66-67 0.1479263298931386 1101 68-69 0.14929118600262825 1101 70-71 0.2301481089453361 1101 72-73 0.1723182518680204 1101 74-75 0.08007253721551422 1101 76-77 0.1589885942741276 1101 78-79 0.07987138281802686 1101 80-81 0.10416971695588018 1101 82-83 0.13067542060051807 1101 84-85 0.10451581662798759 1101 86-87 0.1375694350280412 1101 88-89 0.038222141412866506 1101 90-91 0.08564934195845808 1101 92-93 0.23121647327857175 1101 94-95 0.1860216592160313 1101 96-97 0.13419777583948544 1101 98-99 0.19683864363781822 1101 100 0.10384645607846821 1102 1 0.2066408976005647 1102 2 0.2693662944041648 1102 3 0.2598928143902519 1102 4 0.2947982168659138 1102 5 0.2728846708243964 1102 6 0.32661132582499164 1102 7 0.14782209484976505 1102 8 0.23317611818161055 1102 9 0.24592940768226157 1102 10-11 0.21414608565660131 1102 12-13 0.27411980290578697 1102 14-15 0.4265423067873826 1102 16-17 0.2899756465351899 1102 18-19 0.25309727033493346 1102 20-21 0.4090306169243476 1102 22-23 0.409140308851363 1102 24-25 0.3875453142222014 1102 26-27 0.37712562791338655 1102 28-29 0.3698858131288958 1102 30-31 0.4606447806088809 1102 32-33 0.48741077313702164 1102 34-35 0.4803198350720308 1102 36-37 0.4352191926242881 1102 38-39 0.5368414150243481 1102 40-41 0.4635409905721275 1102 42-43 0.5399925120297766 1102 44-45 0.5213705587702577 1102 46-47 0.5418353656742738 1102 48-49 0.5274172561651085 1102 50-51 0.5459252524003446 1102 52-53 0.6140511571740532 1102 54-55 0.48311228473544077 1102 56-57 0.5489062713800479 1102 58-59 0.47461004558318365 1102 60-61 0.6308267573509241 1102 62-63 0.60129283368682 1102 64-65 0.5896735786069058 1102 66-67 0.6979897787119498 1102 68-69 0.6554224136984317 1102 70-71 0.6324169869112382 1102 72-73 0.585717088553352 1102 74-75 0.41365742137610084 1102 76-77 0.4266379838705916 1102 78-79 0.49023555314055756 1102 80-81 0.43311480912500144 1102 82-83 0.3964451713113988 1102 84-85 0.3993976982350844 1102 86-87 0.46461934982534103 1102 88-89 0.3734416743259743 1102 90-91 0.2044923462729784 1102 92-93 0.19788616131756243 1102 94-95 0.2847309289589717 1102 96-97 0.2874321117504799 1102 98-99 0.1926921985844956 1102 100 0.08645319253020389 1103 1 0.061708551995465655 1103 2 0.14304346167363846 1103 3 0.1626563002188206 1103 4 0.04063117683062245 1103 5 0.07428817579569369 1103 6 0.03463000377510639 1103 7 0.09210433592806311 1103 8 0.07296405136717254 1103 9 0.07282652238799159 1103 10-11 0.04128543864405998 1103 12-13 0.09170083702692011 1103 14-15 0.08676914422876081 1103 16-17 0.15972628881127804 1103 18-19 0.13826918566181945 1103 20-21 0.02840485439072893 1103 22-23 -0.08293845010086187 1103 24-25 0.1140723262523764 1103 26-27 0.15900974939096102 1103 28-29 0.17563286712734794 1103 30-31 0.1436900210697587 1103 32-33 0.1416722467138527 1103 34-35 0.2221922976014099 1103 36-37 0.20672399455510515 1103 38-39 0.19304079485495862 1103 40-41 0.05113843978332966 1103 42-43 0.09065330064339605 1103 44-45 0.14430599439050695 1103 46-47 0.12285890119122911 1103 48-49 0.1465728883528712 1103 50-51 0.25982460928724294 1103 52-53 0.20881753016783478 1103 54-55 0.294561785439015 1103 56-57 0.3108281367329795 1103 58-59 0.1970293462629229 1103 60-61 0.2952789788039709 1103 62-63 0.3059413469247545 1103 64-65 0.2844523589273038 1103 66-67 0.31766385990510315 1103 68-69 0.4103587572198819 1103 70-71 0.37126171709620337 1103 72-73 0.325526094500006 1103 74-75 0.34921366841582113 1103 76-77 0.3795280539719883 1103 78-79 0.30765534125099236 1103 80-81 0.19881327760793255 1103 82-83 0.24691719131280365 1103 84-85 0.33308324537356526 1103 86-87 0.20672568061451102 1103 88-89 0.25913376189806314 1103 90-91 0.2808018362038389 1103 92-93 0.17617210883851087 1103 94-95 0.17868176640428501 1103 96-97 0.16911151233874477 1103 98-99 0.2876134169839659 1103 100 0.3035927326122696 1104 1 0.0156680021800355 1104 2 0.05391687798631395 1104 3 0.09405272753151195 1104 4 0.004261553947614516 1104 5 0.1345262622005663 1104 6 0.07412480056228787 1104 7 0.09968238412038488 1104 8 0.0919288809874601 1104 9 0.16240154696644993 1104 10-11 0.09280025991694885 1104 12-13 0.1583383650834591 1104 14-15 0.23158030748535907 1104 16-17 0.2608464635502017 1104 18-19 0.20910661540453646 1104 20-21 0.09853437821792 1104 22-23 0.14266720016161116 1104 24-25 0.28084526769072937 1104 26-27 0.19873270845160107 1104 28-29 0.16517450210481144 1104 30-31 0.08264107747963578 1104 32-33 0.20802178946181016 1104 34-35 0.2875603252034793 1104 36-37 0.2140972995691115 1104 38-39 0.1400438462934943 1104 40-41 0.1980019041399217 1104 42-43 0.22582368035735811 1104 44-45 0.08359767110496819 1104 46-47 0.16260875743044778 1104 48-49 0.2512636635481016 1104 50-51 0.14589326201684116 1104 52-53 0.16530508326657412 1104 54-55 0.2264064217706121 1104 56-57 0.23874253878088325 1104 58-59 0.2410094794503408 1104 60-61 0.2081722341045733 1104 62-63 0.2910651203206598 1104 64-65 0.23479552982642105 1104 66-67 0.27122982291684394 1104 68-69 0.15814692802624108 1104 70-71 0.17136188589918788 1104 72-73 0.21915818747149274 1104 74-75 0.30575918957184456 1104 76-77 0.21205230780714857 1104 78-79 0.3161346767979296 1104 80-81 0.2383437827183954 1104 82-83 0.20348984151962668 1104 84-85 0.25449386326668133 1104 86-87 0.3091325143528536 1104 88-89 0.26484547051814644 1104 90-91 0.19570032254192782 1104 92-93 0.09999025608665235 1104 94-95 0.1630913733344812 1104 96-97 0.2174802912068401 1104 98-99 0.39345172784115334 1104 100 0.17543880655168564 1105 1 -0.1275985247843039 1105 2 0.09702444877778049 1105 3 0.022087223259845246 1105 4 0.03677010623747634 1105 5 0.06541167047166141 1105 6 0.021564039005497193 1105 7 0.06874324418522093 1105 8 0.10919689452783388 1105 9 0.07492102469467454 1105 10-11 0.12423932820988881 1105 12-13 0.09130968133012374 1105 14-15 0.1581716602695522 1105 16-17 0.11887598909959962 1105 18-19 0.174894321599794 1105 20-21 0.18483347552596285 1105 22-23 0.1463124458048668 1105 24-25 0.11462972312031638 1105 26-27 0.193471930775317 1105 28-29 0.1530329370805461 1105 30-31 0.07680649040747056 1105 32-33 0.06575847744203855 1105 34-35 0.03501747420138912 1105 36-37 0.1481320550004881 1105 38-39 0.08540702756621954 1105 40-41 0.043967327814094403 1105 42-43 0.052309808632749366 1105 44-45 0.05410480846101251 1105 46-47 0.04148191488214081 1105 48-49 0.07639978376374756 1105 50-51 0.08767903387219889 1105 52-53 0.008289742876485207 1105 54-55 0.0795118880719059 1105 56-57 0.0778576230500363 1105 58-59 -0.019736405599729778 1105 60-61 -0.006136731919106353 1105 62-63 0.11451384240258022 1105 64-65 -0.03362726672758498 1105 66-67 0.029103526640447797 1105 68-69 0.017881090618260487 1105 70-71 -0.07022821314618 1105 72-73 -0.023987667130626278 1105 74-75 -0.024363900715265174 1105 76-77 -0.07882136905062254 1105 78-79 -0.12954552129279762 1105 80-81 -0.034359636406499305 1105 82-83 -0.08603203622292455 1105 84-85 -0.017558954959092432 1105 86-87 -0.05781087038377564 1105 88-89 -0.006531000386157615 1105 90-91 0.01895516286933585 1105 92-93 -0.010690927084592516 1105 94-95 0.008346195751332885 1105 96-97 0.02488876827237263 1105 98-99 0.015170290340034143 1105 100 -0.03241228509662264 1106 1 -0.21338119089793395 1106 2 -0.16535158930373584 1106 3 -0.16411017703427078 1106 4 -0.2288699752270773 1106 5 -0.27129232617198085 1106 6 -0.14421477838880747 1106 7 -0.08657121742098184 1106 8 -0.21253393696456868 1106 9 -0.21610586823161526 1106 10-11 -0.1720418467417062 1106 12-13 -0.1679774127610969 1106 14-15 -0.09454920659379695 1106 16-17 -0.15388118921702443 1106 18-19 -0.20930278483940157 1106 20-21 -0.2174363587060526 1106 22-23 -0.10042535282302367 1106 24-25 -0.20128512136411558 1106 26-27 -0.09829458371957855 1106 28-29 -0.03774738642858466 1106 30-31 -0.14222789711955386 1106 32-33 -0.20684462207725574 1106 34-35 -0.21223153663873973 1106 36-37 -0.11759427591743332 1106 38-39 -0.09281966633620442 1106 40-41 -0.09303732835614653 1106 42-43 -0.08434724923802861 1106 44-45 -0.13300167840804988 1106 46-47 -0.25548600508876973 1106 48-49 -0.16886917367106236 1106 50-51 -0.21131379788806015 1106 52-53 -0.2552734170781079 1106 54-55 -0.14313934210968426 1106 56-57 -0.1709570970878218 1106 58-59 -0.15311360046410982 1106 60-61 -0.12068185706966261 1106 62-63 -0.2058086091969571 1106 64-65 -0.1177551568969264 1106 66-67 -0.2306772263822907 1106 68-69 -0.30672522830275284 1106 70-71 -0.23440058029493116 1106 72-73 -0.2103189924553277 1106 74-75 -0.22386647107899904 1106 76-77 -0.17675242236447986 1106 78-79 -0.10896939228095448 1106 80-81 -0.21185949446330454 1106 82-83 -0.19291612952662263 1106 84-85 -0.18060812884969124 1106 86-87 -0.20152716429243966 1106 88-89 -0.13428507762319342 1106 90-91 -0.22653223539206735 1106 92-93 -0.2036424397223051 1106 94-95 -0.13797340138137315 1106 96-97 -0.08637866094348112 1106 98-99 -0.07947418204518897 1106 100 0.03782987649671199 1107 1 0.021094144434822937 1107 2 0.021752296525054504 1107 3 0.007756328831021619 1107 4 0.017460558578846985 1107 5 -0.014355676003624751 1107 6 0.17258163335415588 1107 7 -0.01169696197762704 1107 8 0.031161271946189117 1107 9 0.15647772032651375 1107 10-11 0.06587086888908544 1107 12-13 -0.030165644040756945 1107 14-15 0.04475857526720972 1107 16-17 0.06908331293154646 1107 18-19 0.10922758035925995 1107 20-21 0.16225094004829543 1107 22-23 0.14241490182747896 1107 24-25 0.21479408223417096 1107 26-27 0.17257662909859306 1107 28-29 0.22452682117580025 1107 30-31 0.187829610001927 1107 32-33 0.01885765729895894 1107 34-35 0.12797126043538753 1107 36-37 0.05490137090387037 1107 38-39 0.0746821610788686 1107 40-41 -0.04007699957197275 1107 42-43 0.065427606534179 1107 44-45 -0.13938048608034848 1107 46-47 -0.07571812443863735 1107 48-49 -0.013862140004803791 1107 50-51 -0.08259916903313069 1107 52-53 -0.12755884844006715 1107 54-55 -0.08437414780151897 1107 56-57 -0.10713514836918137 1107 58-59 -0.127676149913043 1107 60-61 -0.04802203561271057 1107 62-63 -0.08897134197282952 1107 64-65 -0.15574155457256467 1107 66-67 -0.1872092107459693 1107 68-69 -0.016814190381168714 1107 70-71 0.031160047231878707 1107 72-73 0.013910900542619231 1107 74-75 -0.11482645361609656 1107 76-77 -0.1832974537190779 1107 78-79 -0.18635979337556563 1107 80-81 -0.15230562628139666 1107 82-83 -0.20770460100473542 1107 84-85 -0.11655576692616876 1107 86-87 -0.0760596471180861 1107 88-89 7.107155585828195E-4 1107 90-91 -0.08819837794884577 1107 92-93 -0.05237115263664904 1107 94-95 -0.11672562396972808 1107 96-97 -0.06085402136660534 1107 98-99 -0.10428709154088978 1107 100 -0.03746296990507858 1108 1 0.11092469102969105 1108 2 0.24503462890305983 1108 3 0.10601456373958129 1108 4 0.20622406922671388 1108 5 0.10114992103527953 1108 6 0.22402973061797127 1108 7 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2214 76-77 -0.20386686679315602 2214 78-79 -0.2270207699676945 2214 80-81 -0.2155064782270273 2214 82-83 -0.19545396873469656 2214 84-85 -0.23194487096002803 2214 86-87 -0.18034168438679288 2214 88-89 -0.25452107883588226 2214 90-91 -0.1969287213600701 2214 92-93 -0.22361759634006617 2214 94-95 -0.16505745579515008 2214 96-97 -0.2046837133653483 2214 98-99 -0.26704681327090896 2214 100 -0.23198723384597386 2215 1 -0.041088754576719566 2215 2 -0.11081285174341815 2215 3 0.0029716464504332407 2215 4 0.013721013407071325 2215 5 0.06493166760596836 2215 6 0.07453020596769022 2215 7 -0.01937166993366901 2215 8 -0.015503551444972175 2215 9 -0.022463317898413493 2215 10-11 -0.026186226569542725 2215 12-13 -0.02713460788951494 2215 14-15 -0.10936563846058789 2215 16-17 -0.09962650942277662 2215 18-19 0.011944453242371367 2215 20-21 0.051710053893600616 2215 22-23 0.07009963259405083 2215 24-25 0.055102024447485576 2215 26-27 -0.01383485911595983 2215 28-29 0.03355288048319238 2215 30-31 0.07014107747964005 2215 32-33 0.0032244921645130376 2215 34-35 0.0841819468251046 2215 36-37 0.10173243470424609 2215 38-39 -0.027726423976773162 2215 40-41 0.08577217441019513 2215 42-43 0.15068854522222352 2215 44-45 0.20508415759145748 2215 46-47 0.19841956824126328 2215 48-49 0.22815555543999722 2215 50-51 0.1387310998546738 2215 52-53 0.1332104886719847 2215 54-55 0.12660912447331185 2215 56-57 0.06286416040249776 2215 58-59 0.06303650647735992 2215 60-61 -0.04243587400353732 2215 62-63 -0.03967812292258088 2215 64-65 0.029660394691283898 2215 66-67 -0.04437828519126441 2215 68-69 -0.0654341530548379 2215 70-71 -0.007827303290000032 2215 72-73 0.030982511795812684 2215 74-75 0.019745676058327888 2215 76-77 -0.0928125570577123 2215 78-79 0.01660764977090423 2215 80-81 0.009154593529210331 2215 82-83 0.0038952469250403965 2215 84-85 -0.015100731327912342 2215 86-87 -0.03924586402553132 2215 88-89 -0.05116804299536781 2215 90-91 0.012659782001389175 2215 92-93 -0.03629352769713279 2215 94-95 -0.049543761800652675 2215 96-97 0.030926237152783642 2215 98-99 -0.074588812699389 2215 100 0.029222590335468368 2216 1 0.051378054337455126 2216 2 -0.06195260992264906 2216 3 -0.08891016957612052 2216 4 -0.10814657786841053 2216 5 0.030092881451395215 2216 6 -0.0984984806136282 2216 7 -0.052415766661972896 2216 8 -0.15236640113677424 2216 9 -0.16531300969547402 2216 10-11 -0.21318895298016116 2216 12-13 -0.16463164439970512 2216 14-15 -0.23037589213531362 2216 16-17 -0.22293880197849347 2216 18-19 -0.20141525515023062 2216 20-21 -0.19714929888023391 2216 22-23 -0.11163875052591266 2216 24-25 -0.10537154122344816 2216 26-27 -0.22134345323640048 2216 28-29 -0.26682674256090877 2216 30-31 -0.2228680500689677 2216 32-33 -0.33110812993126615 2216 34-35 -0.32034922908765395 2216 36-37 -0.2815033998144898 2216 38-39 -0.2818548616249501 2216 40-41 -0.28234126797956094 2216 42-43 -0.25625165155349805 2216 44-45 -0.2941554109244322 2216 46-47 -0.254094952858793 2216 48-49 -0.27304139809248085 2216 50-51 -0.1740510243748119 2216 52-53 -0.18047787074006294 2216 54-55 -0.19623167685433174 2216 56-57 -0.08936764377008899 2216 58-59 -0.1718505845610423 2216 60-61 -0.13569926897745432 2216 62-63 -0.15346872273291723 2216 64-65 -0.12035797894551337 2216 66-67 -0.17017012966731926 2216 68-69 -0.21729611369968893 2216 70-71 -0.14982410272101276 2216 72-73 -0.13360082153751307 2216 74-75 -0.11125865063678475 2216 76-77 -0.22244864050958313 2216 78-79 -0.17881405482842894 2216 80-81 -0.09309439888426141 2216 82-83 -0.1476170219227555 2216 84-85 -0.11227356205337458 2216 86-87 -0.02858382040295737 2216 88-89 -0.17541353849086505 2216 90-91 -0.20343582017973816 2216 92-93 -0.1474700331499541 2216 94-95 -0.12268832083336889 2216 96-97 -0.117525932121751 2216 98-99 -0.06875488666809204 2216 100 -0.1183200839177303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19173.0 3 2893.0 4 1870.0 5 1641.0 6 1290.0 7 1318.0 8 1483.0 9 1630.0 10 1672.0 11 1820.0 12 1974.0 13 2123.0 14 2415.0 15 2463.0 16 2451.0 17 2323.0 18 2253.0 19 2180.0 20 2258.0 21 2419.0 22 2825.0 23 4337.0 24 6806.0 25 9163.0 26 9321.0 27 7384.0 28 7328.0 29 9282.0 30 11368.0 31 13411.0 32 17159.0 33 23815.0 34 34071.0 35 53633.0 36 102079.0 37 207613.0 38 315672.0 39 135863.0 40 252.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.09014076836145 19.47571225445287 12.966366923617068 23.467780053568614 2 15.948690467043575 20.12699975004644 40.1523646871058 23.771945095804185 3 18.325054927313097 25.925983561196176 29.552499229218316 26.19646228227241 4 12.598465786573737 16.933440581083094 38.51007976470336 31.958013867639806 5 12.809310486357367 37.95398444232202 34.64773472194395 14.588970349376662 6 30.12032959337701 38.0857946499432 18.257088280605654 13.536787476074135 7 27.544009803730862 32.675115252193194 21.951701064016028 17.829173880059912 8 25.476741845032517 36.111875678993016 20.361669245664903 18.049713230309568 9 25.183771681557083 17.956715665964452 20.841430180377326 36.018082472101135 10-11 23.92728344343036 27.383911829011147 28.06277864662356 20.62602608093493 12-13 24.610189536584606 24.820023886589276 28.13725452055647 22.432532056269647 14-15 22.130903602095685 26.718695749007736 25.294857942029182 25.85554270686739 16-17 21.863349083876628 29.94835241731208 25.663125104245836 22.525173394565453 18-19 21.797805042789946 28.64074388713316 27.73393450140315 21.827516568673744 20-21 23.074252303000126 28.11172468255944 27.05718792792232 21.756835086518112 22-23 22.740643304002354 27.960163682914196 27.15191243012719 22.14728058295626 24-25 22.351125334381113 27.931673191788995 27.29979561478712 22.41740585904277 26-27 22.199172646669943 27.737586903950863 27.416594819097302 22.64664563028189 28-29 22.30336343792381 27.92107160866269 27.3231616676319 22.4524032857816 30-31 22.57144024228702 28.339567516888703 26.907990842266404 22.18100139855787 32-33 22.061252783344333 28.043029931984037 27.528399959347006 22.367317325324628 34-35 22.24191587648918 27.5237053245806 27.58171650863117 22.65266229029905 36-37 22.70600603141353 28.131457974118018 26.929940493035474 22.23259550143297 38-39 22.48536851502827 28.158825279938384 27.170409876082164 22.18539632895118 40-41 22.06667467394259 27.816304013249933 27.593348385066978 22.523672927740492 42-43 22.086935547716514 27.844448529304493 27.418358722683717 22.650257200295275 44-45 22.24366792520361 28.062067073989134 27.396083168478608 22.29818183232864 46-47 22.252859745309785 28.23359603793606 27.21822961802303 22.29531459873112 48-49 22.103611682819317 27.927951149101066 27.49696529936544 22.471471868714183 50-51 22.135664901584683 27.79094672461219 27.45561681250316 22.617771561299964 52-53 22.214165206433876 27.836049293373776 27.37820977648347 22.57157572370888 54-55 22.34481705448826 28.314413190246405 27.127215144933388 22.213554610331947 56-57 21.912881680038986 28.060808136023162 27.317217170385916 22.709093013551936 58-59 21.868256868419785 27.55586723267837 27.720243412916368 22.85563248598547 60-61 22.358463910892944 28.066884759275464 27.156955191160097 22.4176961386715 62-63 22.126370123323866 27.998587749897506 27.234348412410096 22.640693714368528 64-65 22.125095802582486 27.61487556363042 27.51343181384926 22.74659681993783 66-67 21.9078802756764 27.829417932193568 27.267444235441495 22.995257556688536 68-69 22.439128529752605 28.010091711442804 27.153181306018798 22.397598452785797 70-71 22.505876849250186 27.67123101147943 27.32309395080856 22.499798188461824 72-73 22.10026507888419 27.870435704875923 27.56144446838092 22.46785474785897 74-75 22.119976794183568 27.85660828416524 27.48046203500227 22.542952886648923 76-77 22.232974676834708 27.927987947741123 27.189553671552602 22.649483703871564 78-79 22.27052745422998 27.94733238408689 27.31826210094691 22.46387806073621 80-81 22.115933571619617 27.78632042210713 27.496097454227147 22.601648552046104 82-83 22.080284072977033 27.754935510240603 27.558667615262404 22.606112801519956 84-85 22.174992608384425 27.754008978867763 27.396050543089228 22.674947869658585 86-87 22.289179476456088 27.84022330611709 27.353686350219075 22.51691086720775 88-89 21.97830412735092 27.739702046461147 27.458534896432063 22.82345892975587 90-91 21.966689861516535 27.612374671223943 27.49173227599829 22.92920319126123 92-93 22.198427610567144 27.92226358724194 27.227292354732924 22.652016447457996 94-95 21.992903397412174 27.67086624358065 27.369866037889068 22.966364321118107 96-97 22.072144354841846 27.50281061693528 27.547255320721543 22.877789707501332 98-99 21.85292829054314 27.67401229366105 27.507516385793966 22.965543030001843 100 22.125100412151767 27.639648260851807 27.278650771884987 22.95660055511144 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1340.0 1 916.0 2 452.5 3 333.5 4 220.0 5 172.5 6 161.0 7 153.5 8 154.0 9 173.5 10 164.5 11 147.0 12 160.0 13 160.0 14 169.5 15 209.5 16 255.5 17 319.0 18 389.5 19 534.5 20 813.0 21 1104.0 22 1600.5 23 2474.5 24 3500.0 25 4708.5 26 6531.5 27 8806.5 28 11368.0 29 14086.5 30 16681.0 31 19354.5 32 21550.5 33 23651.5 34 25985.0 35 27917.0 36 29746.0 37 31352.5 38 32344.5 39 32788.5 40 33630.5 41 34578.5 42 34509.5 43 34322.5 44 34106.5 45 33667.5 46 33495.5 47 33142.0 48 32791.5 49 32666.0 50 32029.5 51 31145.0 52 29336.0 53 27607.0 54 26950.5 55 26023.0 56 24456.5 57 22623.5 58 21019.5 59 19666.5 60 17911.5 61 15729.5 62 13808.5 63 11883.0 64 9961.5 65 8062.0 66 6511.0 67 5283.0 68 4116.0 69 3214.5 70 2553.0 71 2060.5 72 1685.5 73 1404.5 74 1162.5 75 867.0 76 642.5 77 539.5 78 430.0 79 314.0 80 227.0 81 160.5 82 112.5 83 82.5 84 67.5 85 43.0 86 31.0 87 27.5 88 23.0 89 17.0 90 12.0 91 7.5 92 7.0 93 7.5 94 4.5 95 1.5 96 1.5 97 3.5 98 3.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07939508139210578 2 0.08163019384255674 3 0.082990697073266 4 0.08571170353468457 5 0.08328223347984658 6 0.08308787587545953 7 0.08269916066668545 8 0.082990697073266 9 0.0828935182710725 10-11 0.08231044545791139 12-13 0.08153301504036321 14-15 0.0800753330074604 16-17 0.07895777678223494 18-19 0.0787634191778479 20-21 0.07842329337017058 22-23 0.08002674360636367 24-25 0.0802211012107507 26-27 0.07895777678223494 28-29 0.07604241271642934 30-31 0.08056122701842802 32-33 0.07934649199100902 34-35 0.07589664451313906 36-37 0.0726897440407529 38-39 0.07473049888681682 40-41 0.07769445235371918 42-43 0.08201890905133082 44-45 0.0828935182710725 46-47 0.08551734593029753 48-49 0.08969603442461889 50-51 0.0912508952597152 52-53 0.09149384226519901 54-55 0.09115371645752168 56-57 0.08862706760049016 58-59 0.0876066901774582 60-61 0.08498286251823317 62-63 0.08595465054016838 64-65 0.08503145191932994 66-67 0.08444837910616881 68-69 0.08362235928752389 70-71 0.08201890905133082 72-73 0.08226185605681462 74-75 0.0816787832436535 76-77 0.08182455144694377 78-79 0.08231044545791139 80-81 0.08318505467765305 82-83 0.08206749845242757 84-85 0.08153301504036321 86-87 0.08221326665571785 88-89 0.07939508139210578 90-91 0.07633394912300989 92-93 0.07647971732630018 94-95 0.07701420073836454 96-97 0.07647971732630018 98-99 0.07555651870546173 100 0.07473049888681682 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1029031.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.70743147263775 #Duplication Level Percentage of deduplicated Percentage of total 1 86.55131825043624 67.25680631812234 2 9.049792628943104 14.06472281110357 3 2.096341701079487 4.887039872396012 4 0.8211915127529091 2.5525073281263366 5 0.4313470152123146 1.6759434312768884 6 0.26481394777850614 1.234680701999816 7 0.16939870380085956 0.9214476717011265 8 0.11774075245464413 0.731946516232485 9 0.09288835982643474 0.6496304270236555 >10 0.39177091078572146 5.081484935531054 >50 0.011395741795449778 0.5674028087491554 >100 0.0017504162836278479 0.22181014407928898 >500 2.5005885072311077E-4 0.15457703365824024 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.717880219351993E-5 0.0 10-11 0.0 0.0 0.0 9.717880219351993E-5 0.0 12-13 0.0 0.0 0.0 9.717880219351993E-5 0.0 14-15 0.0 0.0 0.0 9.717880219351993E-5 0.0 16-17 0.0 0.0 0.0 9.717880219351993E-5 0.0 18-19 0.0 0.0 0.0 1.9435760438703986E-4 0.0 20-21 0.0 0.0 0.0 2.9153640658055976E-4 0.0 22-23 0.0 0.0 0.0 2.9153640658055976E-4 0.0 24-25 0.0 0.0 0.0 4.858940109675996E-4 0.0 26-27 0.0 0.0 0.0 8.260198186449193E-4 0.0 28-29 0.0 0.0 0.0 0.0018949866427736385 0.0 30-31 0.0 0.0 0.0 0.005247655318450076 0.0 32-33 0.0 0.0 0.0 0.01253606548296407 0.0 34-35 0.0 0.0 0.0 0.021913819894638743 0.0 36-37 0.0 0.0 0.0 0.038677163273020926 0.0 38-39 0.0 0.0 0.0 0.07128065140894686 0.0 40-41 0.0 0.0 0.0 0.11525405940151462 0.0 42-43 0.0 0.0 0.0 0.1654955001355644 0.0 44-45 0.0 0.0 0.0 0.21617424547948508 0.0 46-47 0.0 0.0 0.0 0.27297525536159745 0.0 48-49 0.0 0.0 0.0 0.34474180078151195 0.0 50-51 0.0 0.0 0.0 0.42933594809097103 0.0 52-53 0.0 0.0 0.0 0.5113548571423019 0.0 54-55 0.0 0.0 0.0 0.6004192293526629 0.0 56-57 0.0 0.0 0.0 0.7067328389523736 0.0 58-59 0.0 0.0 0.0 0.8249994412218874 0.0 60-61 0.0 0.0 0.0 0.9585717048368805 0.0 62-63 0.0 0.0 0.0 1.0988007164021298 0.0 64-65 0.0 0.0 0.0 1.2540438529062778 0.0 66-67 0.0 4.8589401096759964E-5 0.0 1.424446882552615 0.0 68-69 0.0 9.717880219351993E-5 0.0 1.607240209478626 0.0 70-71 0.0 9.717880219351993E-5 0.0 1.812530429112437 0.0 72-73 0.0 1.9435760438703986E-4 0.0 2.038957038223338 0.0 74-75 0.0 1.9435760438703986E-4 0.0 2.260864833032241 0.0 76-77 0.0 1.9435760438703986E-4 0.0 2.4863196541212074 0.0 78-79 0.0 1.9435760438703986E-4 0.0 2.7449610361592605 0.0 80-81 0.0 1.9435760438703986E-4 0.0 3.033727846877305 0.0 82-83 0.0 1.9435760438703986E-4 0.0 3.3393065903748287 0.0 84-85 0.0 1.9435760438703986E-4 0.0 3.6369166720924833 0.0 86-87 0.0 1.9435760438703986E-4 0.0 3.952456242814842 0.0 88 0.0 1.9435760438703986E-4 0.0 4.204343698100446 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 995 0.0 43.46147 1 GTATCAA 1730 0.0 34.23451 1 TCAACGC 1795 0.0 31.685501 4 ATCAACG 1840 0.0 30.910583 3 CAACGCA 1910 0.0 29.777733 5 AACGCAG 1935 0.0 29.63593 6 CGATATG 40 4.7822087E-4 29.37497 64-65 GTACATG 3920 0.0 28.058825 1 TATCAAC 2120 0.0 27.714931 2 TACATGG 3895 0.0 27.273483 2 ACATGGG 3920 0.0 25.300905 3 ACGCAGA 2315 0.0 24.567043 7 CGCAGAG 2445 0.0 23.451918 8 TACCTGG 710 0.0 22.509209 2 CATGGGG 2755 0.0 22.179993 4 GTACACG 150 3.2884935E-5 21.935444 1 GCAGAGT 2760 0.0 20.60505 9 ACACCGT 185 7.1728937E-6 20.32628 6 GAGTACA 2880 0.0 19.422009 1 ATGGGGC 985 0.0 18.61093 5 >>END_MODULE