FastQCFastQC Report
Wed 25 May 2016
SRR1032911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029031
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32950.32020415322764817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25970.25237334929657124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14230.13828543552137884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20750.056.6119841
GTATCAA36450.040.2201351
ATCAACG44500.032.3721853
TCAACGC44950.032.0481034
TATCAAC46200.031.5855182
CAACGCA46750.030.8051625
AACGCAG47950.030.2305346
TAGCGCG650.00610472628.9705834
ACGCAGA54700.026.4974967
CGCAGAG55500.026.115558
GTACATG37250.025.354571
TACATGG37450.024.7618272
GCAGAGT59100.024.4806549
TCTGTCG2151.8517312E-924.0766268
ACATGGG38000.023.1669353
TACCTGG8900.022.7429472
TAACGCA1050.002313167622.4177114
GAGTACT41400.022.29249612-13
CAGAGTA58900.021.8490110-11
ACTGCGT1100.00303719521.3903928
Produced by FastQC (version 0.11.5)