##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032911_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1029031 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.79565338653549 34.0 31.0 34.0 31.0 34.0 2 32.854232768497745 34.0 31.0 34.0 31.0 34.0 3 32.924141255219716 34.0 31.0 34.0 31.0 34.0 4 36.34904487814264 37.0 37.0 37.0 35.0 37.0 5 36.333244576694 37.0 37.0 37.0 35.0 37.0 6 36.4006468221074 37.0 37.0 37.0 35.0 37.0 7 36.36605699925464 37.0 37.0 37.0 35.0 37.0 8 36.30182764173286 37.0 37.0 37.0 35.0 37.0 9 38.13433123006013 39.0 39.0 39.0 37.0 39.0 10-11 38.14580172997704 39.0 39.0 39.0 37.0 39.0 12-13 38.142431569116965 39.0 39.0 39.0 37.0 39.0 14-15 39.67949507837956 41.0 40.0 41.0 37.0 41.0 16-17 39.68679563589435 41.0 40.0 41.0 37.0 41.0 18-19 39.71928348125567 41.0 40.0 41.0 37.0 41.0 20-21 39.72384602601865 41.0 40.0 41.0 37.0 41.0 22-23 39.676138522551796 41.0 40.0 41.0 37.0 41.0 24-25 39.64954068439143 41.0 40.0 41.0 37.0 41.0 26-27 39.55892242313399 41.0 40.0 41.0 36.5 41.0 28-29 39.48710826010101 41.0 40.0 41.0 36.0 41.0 30-31 39.35224351841684 41.0 39.0 41.0 36.0 41.0 32-33 39.18716199997862 41.0 39.0 41.0 35.0 41.0 34-35 39.069378376356006 41.0 39.0 41.0 35.0 41.0 36-37 38.95463547745403 41.0 39.0 41.0 35.0 41.0 38-39 38.83737661936327 41.0 39.0 41.0 35.0 41.0 40-41 38.714889541714484 41.0 39.0 41.0 35.0 41.0 42-43 38.554325379896234 41.0 38.5 41.0 35.0 41.0 44-45 38.35473761237514 41.0 38.0 41.0 35.0 41.0 46-47 38.07610460714983 41.0 38.0 41.0 34.0 41.0 48-49 37.811014925692234 40.0 38.0 41.0 33.5 41.0 50-51 37.632581039832615 40.0 38.0 41.0 33.0 41.0 52-53 37.470677268226126 40.0 37.0 41.0 33.0 41.0 54-55 37.314653785940365 40.0 37.0 41.0 33.0 41.0 56-57 37.09684353532595 40.0 36.5 41.0 33.0 41.0 58-59 36.92482490809314 40.0 36.0 41.0 32.0 41.0 60-61 36.7262225336263 39.5 35.5 41.0 32.0 41.0 62-63 36.481985479543376 39.0 35.0 41.0 32.0 41.0 64-65 36.18923385204138 39.0 35.0 41.0 31.0 41.0 66-67 35.91466486432381 38.0 35.0 41.0 31.0 41.0 68-69 35.62376740836768 37.0 35.0 40.0 31.0 41.0 70-71 35.30642808622869 37.0 35.0 40.0 31.0 41.0 72-73 34.97433702191674 36.0 35.0 39.0 30.0 41.0 74-75 34.643499564153075 36.0 35.0 39.0 30.0 41.0 76-77 34.30766177112254 35.5 35.0 38.5 30.0 40.0 78-79 33.979713439148085 35.0 34.0 37.0 29.5 39.0 80-81 33.660905745307964 35.0 34.0 37.0 29.0 39.0 82-83 33.367703208163796 35.0 34.0 36.5 29.0 39.0 84-85 33.09924725299821 35.0 34.0 36.0 29.0 37.0 86-87 32.95691091910739 35.0 34.0 36.0 29.0 37.0 88-89 32.77813107671197 35.0 34.0 35.5 29.0 37.0 90-91 32.612421297317574 35.0 34.0 35.0 29.0 36.0 92-93 32.445770341223934 35.0 34.0 35.0 29.0 36.0 94-95 32.3510720279564 35.0 34.0 35.0 29.0 36.0 96-97 32.288014646789065 35.0 34.0 35.0 29.0 36.0 98-99 32.17274941182529 35.0 34.0 35.0 29.0 36.0 100 32.08598671954489 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33756083533951653 1101 2 0.3478634945907899 1101 3 0.3247247810067364 1101 4 0.17350181072204407 1101 5 0.16241507310211745 1101 6 0.1849563716560212 1101 7 0.13034228325174269 1101 8 0.14794072345753762 1101 9 0.19898128727488995 1101 10-11 0.25898251592858657 1101 12-13 0.22610147476267883 1101 14-15 0.34270510350785344 1101 16-17 0.31279375384549013 1101 18-19 0.35577206705511344 1101 20-21 0.2881297039289734 1101 22-23 0.3498082379328409 1101 24-25 0.3920787127792238 1101 26-27 0.34579833116480785 1101 28-29 0.39436546528319383 1101 30-31 0.3281239182978979 1101 32-33 0.2499916840374823 1101 34-35 0.24640506813221918 1101 36-37 0.2982713179283394 1101 38-39 0.323398376812456 1101 40-41 0.44854914857678096 1101 42-43 0.36176369910097605 1101 44-45 0.27462656399568175 1101 46-47 0.3580850920940506 1101 48-49 0.31134162047199254 1101 50-51 0.33214154088982184 1101 52-53 0.37903904950447753 1101 54-55 0.46804763158856844 1101 56-57 0.4062388913682611 1101 58-59 0.4078620939063953 1101 60-61 0.3601446638784367 1101 62-63 0.4053433382078637 1101 64-65 0.48579008144746183 1101 66-67 0.570133532017266 1101 68-69 0.6068450081469052 1101 70-71 0.5974319127840246 1101 72-73 0.5309516361752245 1101 74-75 0.5267642195390323 1101 76-77 0.4524060721354175 1101 78-79 0.46887793297506875 1101 80-81 0.3467736503102756 1101 82-83 0.39078232971179716 1101 84-85 0.38018204705945635 1101 86-87 0.38613354986951975 1101 88-89 0.2907025299183985 1101 90-91 0.2942496123612699 1101 92-93 0.34991561899391854 1101 94-95 0.2831961864077073 1101 96-97 0.3370743526180391 1101 98-99 0.3810176587743257 1101 100 0.3288957636641854 1102 1 0.1307834655908735 1102 2 0.1858323036694074 1102 3 0.22047345117129424 1102 4 0.10846651246080086 1102 5 0.0874765796101542 1102 6 0.10729242767744296 1102 7 0.18233457616256743 1102 8 -0.08947957705678533 1102 9 0.07843992641426922 1102 10-11 0.20754494697655446 1102 12-13 0.21686954213755172 1102 14-15 0.3155966604123748 1102 16-17 0.34108752446635293 1102 18-19 0.2766990413492749 1102 20-21 0.27362633984670737 1102 22-23 0.27033716800447394 1102 24-25 0.22604454882747405 1102 26-27 0.20147661198669198 1102 28-29 0.18347609412573007 1102 30-31 0.26489289726396237 1102 32-33 0.20196144614325817 1102 34-35 0.2563162915808306 1102 36-37 0.30682266485611365 1102 38-39 0.2543588106469912 1102 40-41 0.07855836807861039 1102 42-43 0.147252565775581 1102 44-45 0.21481970737965383 1102 46-47 0.2210558434834482 1102 48-49 0.26204137030236296 1102 50-51 0.23683312783542476 1102 52-53 0.2696843628380563 1102 54-55 0.3214743857541791 1102 56-57 0.33538106333787 1102 58-59 0.21218671360194463 1102 60-61 0.32896254764019517 1102 62-63 0.3410215154398415 1102 64-65 0.3596714399255134 1102 66-67 0.3597724693919844 1102 68-69 0.3740932458235875 1102 70-71 0.4105391438775712 1102 72-73 0.43962340637137487 1102 74-75 0.40287457386830994 1102 76-77 0.47813676945088446 1102 78-79 0.47761237506572485 1102 80-81 0.35909230322710783 1102 82-83 0.37457309881507683 1102 84-85 0.2786012650592369 1102 86-87 0.34253618312498446 1102 88-89 0.29047877571652947 1102 90-91 0.30478237525950647 1102 92-93 0.2868705418453317 1102 94-95 0.20296321270400597 1102 96-97 0.3301333108143325 1102 98-99 0.3031514554998509 1102 100 0.23891187189492058 1103 1 0.15020240882144975 1103 2 0.17629737162906167 1103 3 0.15066106767461207 1103 4 0.09438468909583264 1103 5 0.1433937419954745 1103 6 0.14095616352349083 1103 7 0.1604164428547037 1103 8 0.10558575342614773 1103 9 0.11231192810009105 1103 10-11 0.10961273627651735 1103 12-13 0.16745047089488452 1103 14-15 0.21277143336322268 1103 16-17 0.19785355478159516 1103 18-19 0.1571980226683678 1103 20-21 0.23031123018464683 1103 22-23 0.24393221058819137 1103 24-25 0.24649669741210545 1103 26-27 0.26825830901356085 1103 28-29 0.22769541233328283 1103 30-31 0.23913809360990257 1103 32-33 0.2684314829418142 1103 34-35 0.28520394520765535 1103 36-37 0.2813630275636356 1103 38-39 0.38899258279924 1103 40-41 0.3856547188475261 1103 42-43 0.21816166832213923 1103 44-45 0.17718931967038998 1103 46-47 0.2625805069839515 1103 48-49 0.19960765349073029 1103 50-51 0.06798270603555068 1103 52-53 0.012859594663048313 1103 54-55 0.1673716964169003 1103 56-57 0.2554451383175049 1103 58-59 0.15928842118488973 1103 60-61 0.17260659916408372 1103 62-63 0.1786329967466287 1103 64-65 0.16974677996996945 1103 66-67 0.11478241987099835 1103 68-69 0.12321587058369232 1103 70-71 0.25822246951376826 1103 72-73 0.26671524717552586 1103 74-75 0.18797401465237584 1103 76-77 0.1898451500091909 1103 78-79 0.14142872102924997 1103 80-81 0.14868004518228872 1103 82-83 0.14145814099506282 1103 84-85 0.07796593115937611 1103 86-87 0.043856381760370766 1103 88-89 0.05014295074254704 1103 90-91 0.021402235450608487 1103 92-93 0.11307368326226452 1103 94-95 0.12275821609632942 1103 96-97 0.11339110059679314 1103 98-99 0.1302531433886216 1103 100 0.1514761610486559 1104 1 0.08677051433258498 1104 2 0.1487050401074228 1104 3 0.16015994541302092 1104 4 0.1247311989550397 1104 5 0.12719700304650416 1104 6 0.11430801571885496 1104 7 0.1345719641962262 1104 8 0.052102105055745085 1104 9 0.021256631210889054 1104 10-11 0.1558761153041388 1104 12-13 0.1652467572292622 1104 14-15 0.15956162609541735 1104 16-17 0.1576469102509961 1104 18-19 0.15159483025152554 1104 20-21 0.16833387018909463 1104 22-23 0.19677867407294514 1104 24-25 0.17365496412082138 1104 26-27 0.25803367119798537 1104 28-29 0.2246147578971076 1104 30-31 0.2109929786940299 1104 32-33 0.24341177380233603 1104 34-35 0.1968587709324936 1104 36-37 0.17972438652039102 1104 38-39 0.2665733207094192 1104 40-41 0.20505222204736384 1104 42-43 0.2740978292600218 1104 44-45 0.36774052633347054 1104 46-47 0.3485292817710999 1104 48-49 0.44907944855278004 1104 50-51 0.43873364743679844 1104 52-53 0.5759728967090822 1104 54-55 0.487965428446806 1104 56-57 0.43993242244446407 1104 58-59 0.3671495853689848 1104 60-61 0.3419428880954385 1104 62-63 0.41013770839032304 1104 64-65 0.3278027168265183 1104 66-67 0.36520744166698904 1104 68-69 0.2807327045075283 1104 70-71 0.26348225915928225 1104 72-73 0.2728362102800119 1104 74-75 0.2671766988053008 1104 76-77 0.22065053500983822 1104 78-79 0.2402519694664349 1104 80-81 0.3162450621553319 1104 82-83 0.30657899280087975 1104 84-85 0.24483347646719977 1104 86-87 0.29978976023149784 1104 88-89 0.3955725172342355 1104 90-91 0.34254944649143226 1104 92-93 0.3163525884543503 1104 94-95 0.3119685056583634 1104 96-97 0.30826788369423497 1104 98-99 0.3401526963389614 1104 100 0.35640576463989504 1105 1 0.02862121194868905 1105 2 0.09684637229447901 1105 3 0.06822909806468402 1105 4 0.07728345299835127 1105 5 0.11370229561541123 1105 6 0.06400219299752763 1105 7 0.023441449984197504 1105 8 -0.07115385468132729 1105 9 -0.00964086668462727 1105 10-11 0.06147920845693022 1105 12-13 0.01342973145597881 1105 14-15 0.03787798917819174 1105 16-17 0.045195492630590195 1105 18-19 0.13143314271906092 1105 20-21 0.1307411830685723 1105 22-23 0.18619353625123836 1105 24-25 0.14507912187698224 1105 26-27 0.0662993921873607 1105 28-29 0.1210429455280817 1105 30-31 0.070899754256871 1105 32-33 0.1555719210271036 1105 34-35 0.20632808119702872 1105 36-37 0.1301799862553139 1105 38-39 0.02810324029102418 1105 40-41 0.021141273325277155 1105 42-43 0.09859924419384214 1105 44-45 -0.03319127027427271 1105 46-47 0.21329989234190094 1105 48-49 0.281200685097474 1105 50-51 0.29689884543798684 1105 52-53 0.28260920498555464 1105 54-55 0.26956703502356305 1105 56-57 0.30656282023787895 1105 58-59 0.3596945408612484 1105 60-61 0.4602465794607511 1105 62-63 0.39367902803999044 1105 64-65 0.25448429402997164 1105 66-67 0.33655928555860015 1105 68-69 0.1976675817306699 1105 70-71 0.1268453240134022 1105 72-73 0.12478622192187316 1105 74-75 0.2331504508874076 1105 76-77 0.3010700449770667 1105 78-79 0.28816964360180464 1105 80-81 0.30825314877077403 1105 82-83 0.14666460525229752 1105 84-85 0.12700866855789883 1105 86-87 0.12620670898940745 1105 88-89 0.1758599368606255 1105 90-91 0.13109161689244786 1105 92-93 0.23470172785219745 1105 94-95 0.21087081253780582 1105 96-97 0.19306428546379806 1105 98-99 0.20962818574487585 1105 100 0.18684027469667086 1106 1 0.1139097857164657 1106 2 0.13730516737661702 1106 3 0.025037625578470113 1106 4 0.012254399068169164 1106 5 0.07253400126119658 1106 6 0.06447461462646231 1106 7 0.0836687064584325 1106 8 -0.0439640395715557 1106 9 0.0638900429090441 1106 10-11 0.04494488485852344 1106 12-13 0.03191636994204572 1106 14-15 0.05714152286596885 1106 16-17 0.046648730907485 1106 18-19 0.11728628200412317 1106 20-21 0.050076706877980826 1106 22-23 0.07641206809483947 1106 24-25 0.08650252391082347 1106 26-27 0.07179465258486317 1106 28-29 0.05383143369652288 1106 30-31 0.10653811506650612 1106 32-33 0.010118285742663602 1106 34-35 -0.07173755593721864 1106 36-37 0.056433019613919555 1106 38-39 0.06253605911820159 1106 40-41 0.12168559839296478 1106 42-43 0.08023569537989772 1106 44-45 0.06374878115467197 1106 46-47 -0.020136203531087915 1106 48-49 -0.044507156364765876 1106 50-51 -0.13925077092531524 1106 52-53 -0.0954847774464298 1106 54-55 -0.04612481778372768 1106 56-57 -0.1833052527716248 1106 58-59 -0.26338949687873736 1106 60-61 -0.2121155850277887 1106 62-63 -0.20887113858614725 1106 64-65 -0.23490984040042662 1106 66-67 -0.22279854175265967 1106 68-69 -0.19746846919724703 1106 70-71 -0.23641786987754188 1106 72-73 -0.27119672062341493 1106 74-75 -0.2271498792443012 1106 76-77 -0.22130388671077128 1106 78-79 -0.21894875349327947 1106 80-81 -0.17333410988786113 1106 82-83 -0.2076680861944027 1106 84-85 -0.1490251221575818 1106 86-87 -0.10300684769100599 1106 88-89 -0.045458760937329146 1106 90-91 -0.03673616678698721 1106 92-93 0.007105023361461349 1106 94-95 -0.04742267918246057 1106 96-97 -0.07516436313729002 1106 98-99 -0.1047428368659169 1106 100 -0.03539509442715172 1107 1 0.0774493005356689 1107 2 0.024831742257141798 1107 3 0.042583737491547424 1107 4 0.010671615074485885 1107 5 0.0698388776576877 1107 6 0.05088608995677646 1107 7 0.01911955526555431 1107 8 0.062366845499511214 1107 9 -0.035901037967242644 1107 10-11 -0.04131663371086347 1107 12-13 -0.019430439148699463 1107 14-15 0.04427079178284998 1107 16-17 0.07228021363109605 1107 18-19 0.08980333927205209 1107 20-21 0.02289338494681914 1107 22-23 0.09525451564341836 1107 24-25 0.10660408971700264 1107 26-27 0.11544018256869037 1107 28-29 0.17620112687477985 1107 30-31 0.20619412613303467 1107 32-33 0.1447207874195371 1107 34-35 0.12755794837079293 1107 36-37 0.1039890924027489 1107 38-39 0.2206584800375495 1107 40-41 0.21856055134853136 1107 42-43 0.15576731844229386 1107 44-45 0.14782451057346435 1107 46-47 0.20879834239182316 1107 48-49 0.039979427815929114 1107 50-51 0.07941156960449547 1107 52-53 0.12425519435199561 1107 54-55 -0.017376902375069392 1107 56-57 0.0948315031108038 1107 58-59 0.13828302699474904 1107 60-61 0.10771153397917033 1107 62-63 0.10359091223355676 1107 64-65 0.029880721941616173 1107 66-67 0.02544366894285588 1107 68-69 -0.009140800711250563 1107 70-71 0.08836647841690137 1107 72-73 0.04933271957703056 1107 74-75 0.04983982868709802 1107 76-77 -0.006923426419618295 1107 78-79 -0.03052030536593975 1107 80-81 0.08745453889549992 1107 82-83 0.014388144663740832 1107 84-85 0.14257575199081174 1107 86-87 -0.015520082859673323 1107 88-89 -0.013136095470542841 1107 90-91 -0.09768004130839358 1107 92-93 -0.0013834660144453892 1107 94-95 -0.054912153773443606 1107 96-97 -0.0852026008568103 1107 98-99 -0.2551106616481782 1107 100 -0.3004961500625356 1108 1 0.05885729961032382 1108 2 0.03627804024323922 1108 3 0.03169328780063552 1108 4 0.06033489310144802 1108 5 0.010607297763328916 1108 6 -0.028021876987921246 1108 7 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-0.25171984863021635 2214 52-53 -0.425972913876123 2214 54-55 -0.47482747714660434 2214 56-57 -0.4219398421406524 2214 58-59 -0.32217252054327616 2214 60-61 -0.3827145740411666 2214 62-63 -0.35585271859760326 2214 64-65 -0.47327546903881057 2214 66-67 -0.42221472590960474 2214 68-69 -0.5258980915640024 2214 70-71 -0.5097065389877784 2214 72-73 -0.475377404002046 2214 74-75 -0.48688769530500053 2214 76-77 -0.47696015999233765 2214 78-79 -0.44991050101740626 2214 80-81 -0.5723231596557952 2214 82-83 -0.5173618094370838 2214 84-85 -0.5179003968050679 2214 86-87 -0.5674117693448224 2214 88-89 -0.599268922703196 2214 90-91 -0.5824114492958401 2214 92-93 -0.6045488012492086 2214 94-95 -0.5819521052697567 2214 96-97 -0.5552112256321138 2214 98-99 -0.6145762813866753 2214 100 -0.6885573119592436 2215 1 -0.12052678296470987 2215 2 -0.15737604097365931 2215 3 -0.1607860005329229 2215 4 -0.054998530774689414 2215 5 -0.06604624019674077 2215 6 -0.11880009238925027 2215 7 -0.15704965742539656 2215 8 -0.07843843548479867 2215 9 -0.11820282824857031 2215 10-11 -0.1679752360472122 2215 12-13 -0.1477262157437096 2215 14-15 -0.21618161714781792 2215 16-17 -0.19586660326252314 2215 18-19 -0.18022949407280464 2215 20-21 -0.264706670351444 2215 22-23 -0.20997808268380425 2215 24-25 -0.2537774683116183 2215 26-27 -0.23602038285606852 2215 28-29 -0.1490338907515465 2215 30-31 -0.1448178321167788 2215 32-33 -0.15503417214360837 2215 34-35 -0.19287095879724347 2215 36-37 -0.12385669456068626 2215 38-39 -0.18396721983112485 2215 40-41 -0.14521804822290818 2215 42-43 -0.06218595452376263 2215 44-45 -0.06509731150436693 2215 46-47 -0.041268015526206625 2215 48-49 0.01813350260683677 2215 50-51 0.05947689068005246 2215 52-53 -0.06848656275037257 2215 54-55 -0.1252102472288712 2215 56-57 -0.08777028025824052 2215 58-59 -0.061161225441829004 2215 60-61 0.06680775296029395 2215 62-63 -0.1427676970150884 2215 64-65 -0.24294052641672437 2215 66-67 -0.1462114772519314 2215 68-69 -0.14372675495194187 2215 70-71 -0.23462584894882355 2215 72-73 -0.15324487080106763 2215 74-75 -0.20816113903254063 2215 76-77 -0.1663089244496163 2215 78-79 -0.1500858683713986 2215 80-81 -0.12274142433115287 2215 82-83 -0.17585343963155253 2215 84-85 -0.27307192893820087 2215 86-87 -0.3567642938225575 2215 88-89 -0.35233288817116915 2215 90-91 -0.22245055350856546 2215 92-93 -0.15101227641051196 2215 94-95 -0.11607203488217976 2215 96-97 -0.09558346765711434 2215 98-99 -0.21809054690427843 2215 100 -0.20224288400875778 2216 1 -0.3405410077157782 2216 2 -0.31841207700969676 2216 3 -0.30601774543571736 2216 4 -0.19561315856036998 2216 5 -0.188328720045007 2216 6 -0.25624082259314207 2216 7 -0.22415847677105916 2216 8 -0.1521416123458721 2216 9 -0.28787388088015575 2216 10-11 -0.30909395344882284 2216 12-13 -0.2954707036526756 2216 14-15 -0.39748407091737903 2216 16-17 -0.4108097042582486 2216 18-19 -0.42136628876222915 2216 20-21 -0.36379924977297406 2216 22-23 -0.4008979499194609 2216 24-25 -0.33216977509207624 2216 26-27 -0.46901558200258364 2216 28-29 -0.36563239715267315 2216 30-31 -0.4315760706184406 2216 32-33 -0.536672092959158 2216 34-35 -0.48435566718984546 2216 36-37 -0.44511113979540085 2216 38-39 -0.47624021651201787 2216 40-41 -0.5146134963025659 2216 42-43 -0.5807326591231003 2216 44-45 -0.6620366192331488 2216 46-47 -0.7338216546916883 2216 48-49 -0.6151067771466003 2216 50-51 -0.5887897641326632 2216 52-53 -0.5151099624658784 2216 54-55 -0.47017379630426603 2216 56-57 -0.52608820344458 2216 58-59 -0.5464617233081199 2216 60-61 -0.5657444044602826 2216 62-63 -0.4583426140373632 2216 64-65 -0.5204428972085609 2216 66-67 -0.6332545071239082 2216 68-69 -0.5715580731122003 2216 70-71 -0.6657881296505792 2216 72-73 -0.6314978935606703 2216 74-75 -0.6569742613653986 2216 76-77 -0.7257547518559733 2216 78-79 -0.7145296213348971 2216 80-81 -0.8243340037526821 2216 82-83 -0.7650411470758343 2216 84-85 -0.7657417369040616 2216 86-87 -0.6836828571226974 2216 88-89 -0.6732308255822659 2216 90-91 -0.6099784103127419 2216 92-93 -0.6502636988856239 2216 94-95 -0.6234407522837913 2216 96-97 -0.5395509878088411 2216 98-99 -0.5996395630256615 2216 100 -0.6199396597318483 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 1.0 4 3.0 5 16.0 6 48.0 7 91.0 8 167.0 9 335.0 10 551.0 11 1008.0 12 1590.0 13 2569.0 14 2675.0 15 3164.0 16 3945.0 17 6041.0 18 7626.0 19 4092.0 20 2588.0 21 2394.0 22 2331.0 23 2318.0 24 2589.0 25 3279.0 26 3954.0 27 4811.0 28 6222.0 29 7888.0 30 10243.0 31 12856.0 32 16465.0 33 21352.0 34 30163.0 35 47829.0 36 91872.0 37 202638.0 38 346615.0 39 175987.0 40 711.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25749347928336 19.41327155334937 13.32723696536926 23.001998001998004 2 16.222882176189568 20.124330921996293 39.95372331948207 23.699063582332062 3 18.483249743204517 25.830953257877088 29.972041604269656 25.71375539464874 4 12.60486887017642 17.013735003459992 38.20298103613165 32.17841509023194 5 12.498833846440899 37.33013029822433 34.902587306029794 15.268448549304972 6 30.144373458451813 37.76003451901753 17.886332140587253 14.209259881943407 7 27.10521686991692 32.640640138694124 22.35840264638343 17.895740345005525 8 25.72836699419175 34.95665223687694 20.425321945218705 18.889658823712608 9 25.56539231501817 16.395016617752812 21.481215136732036 36.55837593049698 10-11 24.12211099568429 26.369905279821502 28.297398231928874 21.210585492565336 12-13 25.363024092117865 23.856410336146823 28.740915590855266 22.039649980880043 14-15 22.47565423326262 25.486307966053435 26.63447774552515 25.403560055158795 16-17 21.254121595948032 29.090863151838963 26.92810031962108 22.726914932591924 18-19 21.079258583686293 27.115085510099068 30.202360377073372 21.603295529141263 20-21 22.303506891434758 26.527043402968424 29.945016233718906 21.224433471877912 22-23 21.952129675519664 26.45379629359688 30.010689678629387 21.583384352254065 24-25 21.62762572198092 26.47939581730888 30.06875916217901 21.824219298531183 26-27 21.39171412924691 26.349640440888134 30.251509156985858 22.0071362728791 28-29 21.58691284054823 26.51384059925312 30.19473782675987 21.704508733438782 30-31 21.807759202590386 26.933373765589135 29.69379855697668 21.565068474843798 32-33 21.35601490203168 26.61522102768811 30.255245404348337 21.773518665931867 34-35 21.504787881910254 26.216994915153734 30.235831495329734 22.042385707606275 36-37 21.99999611134011 26.8940204076871 29.398317376865585 21.707666104107204 38-39 21.9219867361021 26.943659787775687 29.465093432071253 21.669260044050958 40-41 21.553382104714565 26.693677611057957 29.743298796661456 22.00964148756602 42-43 21.56302908361381 26.765978385916323 29.56415909194786 22.106833438522003 44-45 21.846518290799512 26.915554171946404 29.362223740949457 21.87570379630463 46-47 21.903219137266348 27.341481508506938 28.770257944470796 21.98504140975592 48-49 21.957316649016924 27.174700339487963 28.68724349298526 22.180739518509853 50-51 21.987544766165197 27.132844476918123 28.526980340344473 22.352630416572215 52-53 22.069985679485086 27.12638243557714 28.47467058924249 22.328961295695287 54-55 22.114612834839562 27.64678068537705 28.160575107287787 22.078031372495605 56-57 21.75722144132138 27.367596776311633 28.338594281211847 22.53658750115514 58-59 21.753428974677966 26.777420368318715 28.776881498175722 22.692269158827596 60-61 22.202200654048703 27.285668707407556 28.231092020100036 22.28103861844371 62-63 21.91241376459345 27.228332632183044 28.361569830921457 22.49768377230205 64-65 21.947395170796604 26.947195954252106 28.623384523887037 22.482024351064254 66-67 21.780063856221947 27.052065026255296 28.37962528807455 22.788245829448204 68-69 22.235039051377562 27.199962294551465 28.31256456329219 22.252434090778785 70-71 22.308317242143335 26.935340140384493 28.326308925581444 22.43003369189072 72-73 21.971398334938403 27.165994027390816 28.46702383115766 22.395583806513116 74-75 21.90905813333126 27.237031731794282 28.265086280199526 22.588823854674935 76-77 22.052002421700458 27.302340361641896 28.027587171984464 22.618070044673182 78-79 22.124843663601972 27.398931616248685 28.06047631218107 22.415748407968273 80-81 21.9233421102372 27.241815444014318 28.21244156491597 22.622400880832515 82-83 21.828890064755253 27.316638946345996 28.239305325362707 22.615165663536043 84-85 21.897591034672427 27.23270727509667 28.07772554957042 22.791976140660488 86-87 21.99438497061293 27.299849177473025 28.01800541095251 22.687760440961533 88-89 21.670519821151437 27.224698671657787 28.128319276364955 22.97646223082582 90-91 21.615852606752284 27.147715395914307 28.129076960378686 23.107355036954726 92-93 21.83234266728149 27.3270286383715 27.93901779506065 22.90161089928636 94-95 21.56711544404471 27.077611859073407 28.084688508850185 23.270584188031695 96-97 21.559190595003535 26.941456860968245 28.22786563041318 23.27148691361504 98-99 21.445272299862687 27.069301119208266 28.208188091515225 23.277238489413826 100 21.59322702620232 27.00666938119454 28.004987216128573 23.39511637647457 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 56.0 1 62.5 2 269.5 3 414.0 4 225.0 5 111.5 6 277.5 7 626.5 8 1044.5 9 1181.5 10 908.0 11 651.0 12 623.0 13 706.0 14 810.0 15 915.5 16 1030.0 17 1157.5 18 1261.5 19 1433.0 20 1660.5 21 1939.5 22 2436.5 23 3174.5 24 4142.5 25 5328.5 26 6915.0 27 8954.0 28 11444.0 29 14163.0 30 16525.5 31 18971.0 32 21367.0 33 23435.5 34 25546.0 35 27484.0 36 29583.0 37 31179.0 38 31979.0 39 32469.0 40 33186.5 41 34019.5 42 34126.5 43 34187.0 44 33783.0 45 33129.5 46 33296.0 47 33062.5 48 32632.5 49 32542.0 50 31822.0 51 30613.0 52 28994.0 53 27523.0 54 26537.5 55 25597.0 56 24040.5 57 22015.0 58 20512.5 59 19412.5 60 17904.0 61 15717.5 62 13677.5 63 11773.0 64 9706.0 65 7759.5 66 6301.0 67 5179.5 68 4036.0 69 3028.0 70 2268.0 71 1789.5 72 1496.0 73 1210.5 74 970.0 75 776.0 76 557.5 77 424.5 78 344.5 79 259.0 80 174.5 81 99.0 82 52.5 83 30.0 84 16.5 85 7.5 86 0.5 87 0.5 88 2.0 89 1.5 90 0.5 91 1.5 92 1.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.915364065805598E-4 2 2.915364065805598E-4 3 1.9435760438703983E-4 4 0.01234170787857703 5 6.802516153546395E-4 6 0.0032069004723861575 7 3.8871520877407966E-4 8 0.14839203094950493 9 0.0010689668241287192 10-11 0.0 12-13 1.457682032902799E-4 14-15 1.9435760438703983E-4 16-17 0.0 18-19 4.858940109675996E-5 20-21 0.0 22-23 9.717880219351991E-5 24-25 4.373046098708396E-4 26-27 0.006073675137094995 28-29 0.008114429983158913 30-31 0.014528230927931228 32-33 0.017297826790446547 34-35 0.027987495031733735 36-37 0.03877434207521445 38-39 0.0436818715859872 40-41 0.05432295042617763 42-43 0.06996873757933435 44-45 0.10918038426441963 46-47 0.11598290041796604 48-49 0.11267882114338636 50-51 0.11564277461028871 52-53 0.1102979404896451 54-55 0.11544841700590167 56-57 0.09873366302861623 58-59 0.09270857729261801 60-61 0.09455497453429489 62-63 0.09314588190248885 64-65 0.0 66-67 4.858940109675996E-5 68-69 1.9435760438703983E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 1.9435760438703983E-4 78-79 0.0 80-81 4.373046098708396E-4 82-83 2.4294700548379983E-4 84-85 0.0 86-87 6.802516153546395E-4 88-89 3.8871520877407966E-4 90-91 1.457682032902799E-4 92-93 9.231986208384393E-4 94-95 0.001263324428515759 96-97 3.4012580767731976E-4 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1029031.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.54331861509095 #Duplication Level Percentage of deduplicated Percentage of total 1 85.78033841413182 64.80131435729085 2 9.535120884309483 14.406293499935982 3 2.220475632897682 5.032262946391059 4 0.864031625867794 2.610872656257833 5 0.45171293653182854 1.706194714349114 6 0.27521413566173475 1.2474353482602776 7 0.18947112988212408 1.0019294553132605 8 0.13979381662001344 0.8448391063476217 9 0.09131152108695162 0.6208177797630163 >10 0.43267054373914227 5.509968774508122 >50 0.014834818179870743 0.7396945891682281 >100 0.004380370173881197 0.6374834350205018 >500 2.576683670725267E-4 0.12895644352398436 >1k 3.8650255060879E-4 0.7119368938701477 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3295 0.32020415322764817 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2597 0.25237334929657124 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1423 0.13828543552137884 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 9.717880219351993E-5 0.0 6 0.0 0.0 0.0 9.717880219351993E-5 0.0 7 0.0 0.0 0.0 9.717880219351993E-5 0.0 8 0.0 0.0 0.0 9.717880219351993E-5 0.0 9 0.0 0.0 0.0 1.9435760438703986E-4 0.0 10-11 0.0 0.0 0.0 1.9435760438703986E-4 0.0 12-13 0.0 0.0 0.0 1.9435760438703986E-4 0.0 14-15 0.0 0.0 0.0 1.9435760438703986E-4 0.0 16-17 0.0 0.0 0.0 2.9153640658055976E-4 0.0 18-19 0.0 0.0 0.0 2.9153640658055976E-4 0.0 20-21 0.0 0.0 0.0 2.9153640658055976E-4 0.0 22-23 0.0 0.0 0.0 2.9153640658055976E-4 0.0 24-25 0.0 0.0 0.0 5.344834120643595E-4 0.0 26-27 0.0 0.0 0.0 9.231986208384393E-4 0.0 28-29 0.0 0.0 0.0 0.0019921654449671583 0.0 30-31 0.0 0.0 0.0 0.005296244719546836 0.0 32-33 0.0 0.0 0.0 0.012487476081867309 0.0 34-35 0.0 0.0 0.0 0.021816641092445223 0.0 36-37 0.0 0.0 0.0 0.03853139506973065 0.0 38-39 0.0 9.717880219351993E-5 0.0 0.0714750090133339 0.0 40-41 0.0 9.717880219351993E-5 0.0 0.11559418520919196 0.0 42-43 0.0 9.717880219351993E-5 0.0 0.165981394146532 0.0 44-45 0.0 1.9435760438703986E-4 0.0 0.2166115500893559 0.0 46-47 0.0 2.9153640658055976E-4 0.0 0.27404422218572616 0.0 48-49 0.0 2.9153640658055976E-4 0.0 0.34547064179796333 0.0 50-51 0.0 2.9153640658055976E-4 0.0 0.43059927251948676 0.0 52-53 0.0 2.9153640658055976E-4 0.0 0.5128611285763014 0.0 54-55 0.0 2.9153640658055976E-4 0.0 0.6029944676107911 0.0 56-57 0.0 2.9153640658055976E-4 0.0 0.709502434814889 0.0 58-59 0.0 4.858940109675996E-4 0.0 0.8284978781008541 0.0 60-61 0.0 4.858940109675996E-4 0.0 0.963333466144363 0.0 62-63 0.0 4.858940109675996E-4 0.0 1.1044856763304507 0.0 64-65 0.0 4.858940109675996E-4 0.0 1.2603604750488566 0.0 66-67 0.0 4.858940109675996E-4 0.0 1.4308120940962907 0.0 68-69 0.0 4.858940109675996E-4 0.0 1.6154032288628817 0.0 70-71 0.0 4.858940109675996E-4 0.0 1.8233658655570144 0.0 72-73 0.0 4.858940109675996E-4 0.0 2.0525620705304313 0.0 74-75 0.0 4.858940109675996E-4 0.0 2.277628176410623 0.0 76-77 0.0 4.858940109675996E-4 0.0 2.5055124675544276 0.0 78-79 0.0 4.858940109675996E-4 0.0 2.7667776772517056 0.0 80-81 0.0 4.858940109675996E-4 0.0 3.0567106335960723 0.0 82-83 0.0 4.858940109675996E-4 0.0 3.3645730789451433 0.0 84-85 0.0 4.858940109675996E-4 0.0 3.663397895690217 0.0 86-87 0.0 4.858940109675996E-4 0.0 3.98068668485206 0.0 88 0.0 4.858940109675996E-4 0.0 4.2344691267804375 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2075 0.0 56.611984 1 GTATCAA 3645 0.0 40.220135 1 ATCAACG 4450 0.0 32.372185 3 TCAACGC 4495 0.0 32.048103 4 TATCAAC 4620 0.0 31.585518 2 CAACGCA 4675 0.0 30.805162 5 AACGCAG 4795 0.0 30.230534 6 TAGCGCG 65 0.006104726 28.970583 4 ACGCAGA 5470 0.0 26.497496 7 CGCAGAG 5550 0.0 26.11555 8 GTACATG 3725 0.0 25.35457 1 TACATGG 3745 0.0 24.761827 2 GCAGAGT 5910 0.0 24.480654 9 TCTGTCG 215 1.8517312E-9 24.076626 8 ACATGGG 3800 0.0 23.166935 3 TACCTGG 890 0.0 22.742947 2 TAACGCA 105 0.0023131676 22.417711 4 GAGTACT 4140 0.0 22.292496 12-13 CAGAGTA 5890 0.0 21.84901 10-11 ACTGCGT 110 0.003037195 21.390392 8 >>END_MODULE