FastQCFastQC Report
Wed 25 May 2016
SRR1032910_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032910_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2517021
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25900.030.3059021
GTATCAA37100.030.2785111
GTACATG91850.029.628521
TACATGG89150.029.0496432
TCAACGC38100.027.8800774
ACATGGG92000.027.1279663
ATCAACG40300.026.5913473
CAACGCA40550.026.1945465
AACGCAG43150.024.7246256
TATCAAC47400.023.004892
GAGTACA64000.021.5178171
CATGGGA32850.020.746424
ACGCAGA51900.020.4656587
CATGGGG55900.020.2635614
CGCGCGT2351.267399E-719.9993927
GTATAGA9900.019.9399761
TATACCG1656.8635185E-519.9391825
AGTACAT66400.019.3951442
CGCAGAG54550.019.2991378
TACACCG4900.019.1834725
Produced by FastQC (version 0.11.5)