FastQCFastQC Report
Wed 25 May 2016
SRR1032910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2517021
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57950.23023248514811756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48460.192529184301601No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25190.10007862469164938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42050.046.6959421
GTATCAA72750.040.356721
TCAACGC87900.032.9078144
CAACGCA89900.032.2771455
ATCAACG90000.032.1399653
AACGCAG92900.031.2348236
GTACATG90100.031.125651
TATCAAC95050.030.6303062
TACATGG90350.029.7769812
TATACCG1154.178597E-628.6266025
ACATGGG93650.027.7734723
ACGCAGA109850.026.3708637
CGCAGAG112600.025.601528
TATAACG2301.5279511E-1024.539532
TACACCG4450.023.2504985
GCAGAGT124250.023.1724829
CATGGGG59750.022.5133914
ACCGATC2957.2759576E-1222.3177158
ACACCGT5000.021.6335336
TAACGCA2458.434654E-921.117354
Produced by FastQC (version 0.11.5)